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OfflineGreenfingersUK
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Wine cap culture
    #28601796 - 12/29/23 05:07 AM (30 days, 11 hours ago)

Hello everyone, I'm hoping to start a mushroom bed outdoors this coming spring, I have a syringe of wine cap culture, which is the best grain to shoot the culture into? I have a pressure cooker if needed.


Edited by GreenfingersUK (12/29/23 05:08 AM)


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Offlinewtfcrazymofo
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Re: Wine cap culture [Re: GreenfingersUK]
    #28601815 - 12/29/23 05:36 AM (30 days, 11 hours ago)

all of them. the industry standard is rye.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




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OfflineGreenfingersUK
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Re: Wine cap culture [Re: wtfcrazymofo]
    #28612460 - 01/06/24 11:49 PM (21 days, 17 hours ago)

Thanks, what would you do to make up enough spawn for putting in an outdoor bed of 3x3m
i have a few months yet before the weather is warm enough here.


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OfflineChtouxhu
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Re: Wine cap culture [Re: GreenfingersUK]
    #28614195 - 01/08/24 03:02 PM (20 days, 1 hour ago)

Usually a 1 kg (approximately 6 l) spawn bag is used to colonize a 1 x 1 m. You could probably get away with less since Stropharia rugosoannulata is an extremely aggressive fungus when grown outdoors.
I think several spawn bags of sterilized material would be your best option. Bags are usually difficult to use without a laminar flow hood (don't know if you have one) but since you have a liquid culture you don't need to open the bags, after sterilization you can just poke them with the syringe and rapidly cover the hole with tape (or better yet, use bags with self-healing injection ports). Obviously don't do this in open air, a still air box is at least mandatory. If you feel more comfortable with canning jars, they'll work too as long as you reach the required spawn quantity, but you'd need a whole lot of them.

Keep in mind that Stropharia rugosoannulata likes disturbance, and its spawn will need to be shaken and disrupted regularly (Paul Stamets, whose family has been growing this species for a long time, suggests shaking it once a week), otherwise it will stall.

As for spawn material, grains will work but they might attract mice and rats when used outdoors. This is why sawdust is usually preferred. Depending on how many rodents you have in your area this may or may not be a problem.


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OfflineGreenfingersUK
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Re: Wine cap culture [Re: Chtouxhu]
    #28615832 - 01/09/24 10:57 PM (18 days, 18 hours ago)

Great info, thank you :smile:


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OfflineGreenfingersUK
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Re: Wine cap culture [Re: Chtouxhu]
    #28621642 - 01/14/24 11:41 PM (13 days, 17 hours ago)

Quote:

Chtouxhu said:
Usually a 1 kg (approximately 6 l) spawn bag is used to colonize a 1 x 1 m. You could probably get away with less since Stropharia rugosoannulata is an extremely aggressive fungus when grown outdoors.
I think several spawn bags of sterilized material would be your best option. Bags are usually difficult to use without a laminar flow hood (don't know if you have one) but since you have a liquid culture you don't need to open the bags, after sterilization you can just poke them with the syringe and rapidly cover the hole with tape (or better yet, use bags with self-healing injection ports). Obviously don't do this in open air, a still air box is at least mandatory. If you feel more comfortable with canning jars, they'll work too as long as you reach the required spawn quantity, but you'd need a whole lot of them.

Keep in mind that Stropharia rugosoannulata likes disturbance, and its spawn will need to be shaken and disrupted regularly (Paul Stamets, whose family has been growing this species for a long time, suggests shaking it once a week), otherwise it will stall.

As for spawn material, grains will work but they might attract mice and rats when used outdoors. This is why sawdust is usually preferred. Depending on how many rodents you have in your area this may or may not be a problem.




ive done around 1kg of rye in a bag and inoculated last friday, i will keep you up to date on how well it does.


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OfflineChtouxhu
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Re: Wine cap culture [Re: GreenfingersUK]
    #28621691 - 01/15/24 02:16 AM (13 days, 14 hours ago)

Quote:

GreenfingersUK said:
ive done around 1kg of rye in a bag and inoculated last friday, i will keep you up to date on how well it does.




Great, by all means keep us updated!
Personally I would've waited until mid-February but wine cap mycelium is a tank so it will probably bear the cold fine. I'd strongly recommend laying a black tarp over the patch to trap heat during sunny days so that mycelium has a chance to recover and grow faster. Having lots of leaf litter and mulch in general helps too.
You're not going to get much growth in the dead of winter but all that matters is that grains recover after spawning them.


EDIT: I'm sorry I thought you said you spawned colonized rye outdoors to inoculate the patch, my bad :facepalm3:.

Keep us updated on how well it colonizes! Wine cap is generally slow-growing in sterile media so don't be alarmed if you see sluggish growth and stalling. As soon as you get a nice 20%-30% colonization, shake it.


Edited by Chtouxhu (01/15/24 02:26 AM)


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OfflineGreenfingersUK
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Re: Wine cap culture [Re: Chtouxhu]
    #28621881 - 01/15/24 08:37 AM (13 days, 8 hours ago)

Quote:

Chtouxhu said:
Quote:

GreenfingersUK said:
ive done around 1kg of rye in a bag and inoculated last friday, i will keep you up to date on how well it does.




Great, by all means keep us updated!
Personally I would've waited until mid-February but wine cap mycelium is a tank so it will probably bear the cold fine. I'd strongly recommend laying a black tarp over the patch to trap heat during sunny days so that mycelium has a chance to recover and grow faster. Having lots of leaf litter and mulch in general helps too.
You're not going to get much growth in the dead of winter but all that matters is that grains recover after spawning them.


EDIT: I'm sorry I thought you said you spawned colonized rye outdoors to inoculate the patch, my bad :facepalm3:.

Keep us updated on how well it colonizes! Wine cap is generally slow-growing in sterile media so don't be alarmed if you see sluggish growth and stalling. As soon as you get a nice 20%-30% colonization, shake it.




Thank you and i will keep this post updated as i go.
Cheers


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