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Offlineonions_have_layers
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Registered: 12/12/23
Posts: 27
Last seen: 5 hours, 32 seconds
Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos]
    #28618058 - 01/11/24 08:11 PM (16 days, 2 hours ago)

I had way more condensation issues when I would seal my dishes. Now I use ziplock sandwich bags to store 4 plates at a time and only really deal with condensation when there are large temperature swings in my room




Damn I may have to try this


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InvisibleinskiM
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Registered: 02/28/06
Posts: 5,720
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SupaThaRipper] * 1
    #28618142 - 01/11/24 09:37 PM (16 days, 54 minutes ago)

Quote:

SupaThaRipper said:
Gotta get colder! Haha try starting your pour around 115ish. Plastic is just part of the condensation game. I know some guys cool for hours in front off their ffu with them unwrapped



I do this but it definitely doesn't take hours, I pour while still very hot with a good glove directly from the PC then leave the lids off to cool, I use a laser thermometer to check the temperature and within 15min they are gelled and cooled to the same temperature as the inside of the hood then I put the lids on and use straight away, I have no condensation at all.
Here's an image of 20 plates cooling.


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OfflineSupaThaRipper
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Registered: 09/02/13
Posts: 1,488
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Re: AGAR ENVY! (Anything and All things agar!) [Re: inski] * 1
    #28618170 - 01/11/24 10:21 PM (16 days, 11 minutes ago)

Very nice! 😁 I’m down to almost no condensation with my condiment cups. Probably 95% of them but my shits nearly solidified while finishing my pours lol. Once I get my ffu, this will be something worth playing around with


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OfflinemorningWood
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Registered: 12/25/23
Posts: 123
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Re: AGAR ENVY! (Anything and All things agar!) [Re: SupaThaRipper]
    #28618197 - 01/11/24 11:01 PM (15 days, 23 hours ago)

Testing vendor lc on agar. This doesn't look quite right to me. Should I transfer and see?? Where would you take from?


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OfflineShroomadillo
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Registered: 12/05/23
Posts: 15
Last seen: 4 days, 18 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit]
    #28618270 - 01/12/24 03:08 AM (15 days, 19 hours ago)

Should I transfer this one more time or send it sometime next week after it has grown some more?



Edited by Shroomadillo (01/12/24 08:54 AM)


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OfflineSupaThaRipper
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Registered: 09/02/13
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Re: AGAR ENVY! (Anything and All things agar!) [Re: morningWood] * 8
    #28618852 - 01/12/24 03:39 PM (15 days, 6 hours ago)

Looks clean to me.

And send that plate Shroomadillo lol.

On a side not. G2A always gets so rhizooo


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: AGAR ENVY! (Anything and All things agar!) [Re: SupaThaRipper] * 1
    #28618911 - 01/12/24 04:31 PM (15 days, 6 hours ago)

Quote:

SupaThaRipper said:
Looks clean to me.

And send that plate Shroomadillo lol.

On a side not. G2A always gets so rhizooo






:mygoditsfullofstars: I don't think I've ever seen rhizo like THAT before! Straight up ropes, bro!


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I :heart: 5318008


NOT a virgin!


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Onlinehuey.bluey
I'm a teapot Unread Journal User Gallery

Registered: 01/16/19
Posts: 1,447
Loc: Mississippi River
Last seen: 4 minutes, 58 seconds
Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl] * 1
    #28619388 - 01/13/24 05:29 AM (14 days, 17 hours ago)

TAT T5, Im going to send one of these 3.


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:mushroom2: huey.bluey's Trading List


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OfflineMistaK
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Registered: 04/30/23
Posts: 60
Last seen: 4 days, 43 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: huey.bluey] * 1
    #28620903 - 01/14/24 11:41 AM (13 days, 10 hours ago)

Nice plates man! I think I'd send the plate from the rightmost picture but it's a little unclear because of the wrap.


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Offline99.99
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Registered: 12/22/14
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MistaK]
    #28621202 - 01/14/24 03:40 PM (13 days, 6 hours ago)

So im trying something new
I have a really nice hepa filter so I’ve never needed to work with liquid cultures but I’m kind of curious how fast things will colonize using a liquid culture
Anyways, I made up my first liquid culture today, and I added food coloring to it because I have noticed the mycelium eat the food coloring and that gives me a good indication when everything is fully colonized and being able to keep things sterile from the heap filter. I don’t have to notice contamination.



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Offlinenogoodnamesleft
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Registered: 09/19/20
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Loc: Melmak
Last seen: 13 days, 1 hour
Re: AGAR ENVY! (Anything and All things agar!) [Re: 99.99] * 1
    #28621514 - 01/14/24 08:52 PM (13 days, 1 hour ago)



Am I doing it right


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OfflineSupaThaRipper
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Registered: 09/02/13
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Re: AGAR ENVY! (Anything and All things agar!) [Re: nogoodnamesleft] * 2
    #28621563 - 01/14/24 09:39 PM (13 days, 52 minutes ago)

No. Needs more colorful fun stuff, like this:



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OfflineMycobeachgirl
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Registered: 08/07/23
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Last seen: 3 days, 19 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: SupaThaRipper]
    #28622473 - 01/15/24 06:02 PM (12 days, 4 hours ago)

Second generation plate pin question:

Hi All!
So I had a plate pin that everyone said to clone
I tried microsurgery and it didn’t go so well
I wound up just putting a part of the cap and a part of the stem on agar
And what do you know?
They grew!
And there’s another pin!

So my next question is:
Do I do the same thing again?
(Try to clone the pin and a piece to new agar?)
If you’d take a piece to new agar where would you take from?
Thanks for any feedback!, as always!!




Edited by Mycobeachgirl (01/15/24 06:03 PM)


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycobeachgirl] * 1
    #28622530 - 01/15/24 06:49 PM (12 days, 3 hours ago)

Quote:

Mycobeachgirl said:
Second generation plate pin question:

Hi All!
So I had a plate pin that everyone said to clone
I tried microsurgery and it didn’t go so well
I wound up just putting a part of the cap and a part of the stem on agar
And what do you know?
They grew!
And there’s another pin!

So my next question is:
Do I do the same thing again?
(Try to clone the pin and a piece to new agar?)
If you’d take a piece to new agar where would you take from?
Thanks for any feedback!, as always!!








Hello! Yes, plate pins are wonderful little things to work with :smile: in the future, leave microsurgery to the surgeons. As you found out you can simply pluck the pin off the plate with your scalpel/tweezers, place it on a new plate and mycelium will grow from all around it! From there, simply take a clean transfer piece and put it to another plate or LC if you are absolutely certain it is clean.

If I were you I would take a transfer piece from the thick rhizo growth on the left side of your 3rd picture. Just a small piece to a new plate and let it grow out. That plate has a LOT going on with it - not all of it good. But that's my suggestion. Transfer from the obvious rhizo on the left side and go from there


--------------------



I :heart: 5318008


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OfflineMushBiskit
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Registered: 01/07/24
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Loc: Argentina
Last seen: 1 day, 11 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #28623163 - 01/16/24 09:16 AM (11 days, 13 hours ago)

hello everyone, I'm trying to grow for the first time and i bought a liquid mycelium syringe of mckennaii and i put it in some PDA to see if it was clean and this is the result after 4 days.


this is the under side of the "plate", it has a faint pink/orange color that is not visible from the top side.



this is one of five poured cups and all look the same, 10 days before this i did another 5 cups with the same results, i throw those and started over thinking it was my technique. i use a SAB to inoculate and i left one cup open the entire process to check if the contams were inside the SAB and that one is clean, not a single spot of contamination on that cup. the agar was left 7 days after poured to make sure it was clean before i do anything with them.
the cups are stored in a modified monotub with temp control at 26°C (78.8°F)

So the question is: is this contamination? should i try some water agar maybe? or perhaps lowering temp would help?

PS: english is not my fist language, have mercy with my grammar.
PS 2: im from Argentina, so if there are some Argentinians message me, im having a really hard time finding basic materials for the hobbie.

thank to everyone for sharing your knoledge


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OfflineMycobeachgirl
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Registered: 08/07/23
Posts: 79
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MushBiskit] * 1
    #28625102 - 01/17/24 06:57 PM (10 days, 3 hours ago)

Hi again
I’m back with a photo dump of my current situation
Any advice on any plates is greatly appreciated!
I love the knowledge all of you share - thank you!
I have no idea what I’m doing, but I’m trying!





[url=https://files.shroomery.org/files/24-003/554290859-DA9C6FD1-398F-4842-A072-8692105C98D2.jpg][image]https://www.shroomery.org/forums/thumbs/24-003/554290859-thumb_DA9C6FD1-398F-4842

-A072-8692105C98D2.jpg[/image][/url]


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OfflineSupaThaRipper
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Registered: 09/02/13
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycobeachgirl] * 1
    #28625258 - 01/17/24 08:24 PM (10 days, 2 hours ago)

Who doesn’t enjoy some good plate pins (is it “pins” or, more properly, “plins?”) 🤷‍♂️

Shakti


Iceberg


Toque


Ape 2


Cassini


TTBVI (stones, never really grow past this)


Hillbilly Pumpkin



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InvisibleHILLBILLY OUTLAWS
Above And Beyond!
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Registered: 04/21/22
Posts: 2,852
Loc: Luckenbach Texas
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28625267 - 01/17/24 08:29 PM (10 days, 2 hours ago)

That’s a plate pumpking. A plumkin you could say.
:curbyourenthusiasm:


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TEAM SPREAD THE LOVE!
Smellyhobbit said:
Embarrassment and bashfulness are leeches on your ability to learn.


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycobeachgirl] * 1
    #28625269 - 01/17/24 08:31 PM (10 days, 2 hours ago)

Quote:

Mycobeachgirl said:
Hi again
I’m back with a photo dump of my current situation
Any advice on any plates is greatly appreciated!
I love the knowledge all of you share - thank you!
I have no idea what I’m doing, but I’m trying!

Trying is more than a lot of people do so you're at least one step ahead. Let's get you across the finish line :rockon:



Ew :lol: take a transfer from the normal and uniform growth, there's a ray extending from the transfer piece straight up to 12 o'clock. Take a small transfer from there.



Same story, only 9 o'clock.



Is this an LC test plate? Doesn't look terrible. I would take a transfer from the leading edge of the band of mycelium oriented from like 1 o'clock going towards about 8 o'clock but I would focus my efforts on the right half of that plate...does that make any sense? I didn't think so :lol: I'm too lazy to edit a picture right now...you any good at Algenbra/Trig/Calc? lol what a stupid question...nobody is..I would transfer from (0,8), (1,9), (2,10)



I don't have krinkle brain yet so feel free to send me this plate, I'll clean it up and send you a clean one :smile: Or you could get the satisfaction of doing it yourself and take transfers from the leading edges of (8,-1), (9,-2)



Nice. Transfer from pretty much anywhere on the bigger colony in Quadrant 1. But, I like the leading edge from 9-12 o'clock the most. Like I said though I think anywhere will do for that one.

[url=https://files.shroomery.org/files/24-003/554290859-DA9C6FD1-398F-4842-A072-8692105C98D2.jpg][image]https://www.shroomery.org/forums/thumbs/24-003/554290859-thumb_DA9C6FD1-398F-4842


This is a crapshoot. Personally I think you're going to have a very hard time cleaning this one up. Try taking the smallest transfer you can from 6-7 o'clock and report back with what it looks like.



I would aim for about 10-12 o'clock, 3-4 and 5-7 o'clock.


Based on the growth patterns of your transfer plates and your (either) LC test or germ plates, it looks like you're still struggling with your sterile technique a little bit. Take the smallest transfer pieces you can. Move slow but deliberately - no wasted movements no extra movements. An old trap shooting coach of mind told me to pretend I was moving underwater when I was struggling to break doubles. Slow is smooth, smooth is fast. Doubles all day! I also sterilize my tools fairly frequently, wipe down my forearms with ISO as well as my gloved hands, obviously. I also wear a covid mask because I was noticing my breath would go into the armholes when I exhaled and caused me all sorts of stupid frustrations.


Hopefully I was some sort of help for you. If you've got any further questions just hollar!





--------------------



I :heart: 5318008


NOT a virgin!


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Offlinejack1
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Registered: 12/29/22
Posts: 19
Last seen: 1 day, 12 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: bigfootscreepyuncl]
    #28625769 - 01/18/24 10:48 AM (9 days, 11 hours ago)

Hi all. I’ve been growing out a number of strains on agar and a couple of them don’t look quite right to me. For example this plate



Maybe it’s just me but I feel like the strands should look more like this



Any thoughts?  Thanks.


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