Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
OfflineWhoLady1953
Stranger
 User Gallery


Registered: 05/17/23
Posts: 17
Last seen: 6 days, 1 hour
what does agar contam look like... like this?
    #28611596 - 01/06/24 11:01 AM (22 days, 5 hours ago)




Does this look contaminated? I have not used agar much. This was a drizzle of Golden Oyster liquid culture on this, done on Christmas day. Is this telling me to ditch the liquid culture or should I wait longer?


Extras: Filter Print Post Top
OfflineMrmeowgi
Storyteller
Male User Gallery


Registered: 08/10/21
Posts: 20
Loc: United States
Last seen: 5 days, 1 hour
Re: what does agar contam look like... like this? [Re: WhoLady1953]
    #28611612 - 01/06/24 11:12 AM (22 days, 5 hours ago)

Yeah, that’s a whole lot of bacteria. Looks like there’s some myc in there as well.


Extras: Filter Print Post Top
OfflineWhoLady1953
Stranger
 User Gallery


Registered: 05/17/23
Posts: 17
Last seen: 6 days, 1 hour
Re: what does agar contam look like... like this? [Re: Mrmeowgi]
    #28612077 - 01/06/24 06:20 PM (21 days, 22 hours ago)

Ugh, I thought so. I will try another agar with the same liquid culture, in case I was less than sterile when I did the first one.
Thanks for the response.


Extras: Filter Print Post Top
InvisibleCrayolaHalls
Male


Registered: 03/23/10
Posts: 216
Loc: The Lot
Re: what does agar contam look like... like this? [Re: WhoLady1953]
    #28612393 - 01/06/24 10:03 PM (21 days, 18 hours ago)

Quote:

WhoLady1953 said:
Ugh, I thought so. I will try another agar with the same liquid culture, in case I was less than sterile when I did the first one.
Thanks for the response.




I am thinking maybe it was your plate or process. That plate is covered really well.  It helps to do really small amounts of LC in one (or very few) spots on a plate.  Any place you did not put drops that gets contams you'll know wasn't from the LC.

I do this by having small dots drawn on the bottom of my plates.  I put one drop of LC over each and an cautious to not have the drops roll around.  The dots remind me exactly where I should be seeing myc growth.


--------------------

.


Extras: Filter Print Post Top
OfflineWhoLady1953
Stranger
 User Gallery


Registered: 05/17/23
Posts: 17
Last seen: 6 days, 1 hour
Re: what does agar contam look like... like this? [Re: CrayolaHalls]
    #28612679 - 01/07/24 07:16 AM (21 days, 9 hours ago)

Crayolahalls that is an excellent tip! Will try post haste thanks!


Extras: Filter Print Post Top
Offlinesyri
Etard
Female

Registered: 09/27/23
Posts: 84
Last seen: 2 hours, 35 minutes
Re: what does agar contam look like... like this? [Re: WhoLady1953]
    #28612865 - 01/07/24 10:48 AM (21 days, 6 hours ago)

:bonghit:


Extras: Filter Print Post Top
Offlinewtfcrazymofo
foil hater
Other


Registered: 07/26/15
Posts: 1,197
Loc: Colonial alley
Last seen: 4 hours, 9 minutes
Re: what does agar contam look like... like this? [Re: syri]
    #28615930 - 01/10/24 02:50 AM (18 days, 14 hours ago)

Bigtime contaminated city

I also like to keep a blank uninoculated petri to see how I did.

It was hard for me to get one drop so I squirt some lc on a inoculation loop first then touch the agar with that.


--------------------
If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541

Bag sealers are to bulky (my hood isn't that big)
https://www.shroomery.org/forums/showflat.php/Number/28622922




Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Cultivating yeast cultures. Baby_Hitler 2,531 13 07/14/04 12:54 PM
by blackmold
* Culture Syringe wideweb 871 3 11/29/04 02:13 PM
by ragadinks
* Liquid and agar culture of H. ulmatius? Baby_Hitler 1,541 4 05/19/04 08:41 AM
by charlieXxXxX
* Maintaining clean viable cultures for mass distribution? Baby_Hitler 1,203 1 09/27/04 03:19 AM
by ragadinks
* Wine cap culture GreenfingersUK 184 7 01/15/24 08:37 AM
by GreenfingersUK
* Should A. bisporus be any color than white on peroxide agar? Baby_Hitler 1,961 8 08/16/03 11:35 AM
by Mycena
* Oysters, coffee grounds, and liquid culture psyconaut 5,268 10 11/07/02 12:51 PM
by psyconaut
* Legal issues with selling mushroom cultures.
( 1 2 all )
Baby_Hitler 7,438 23 02/05/09 05:35 AM
by Chakravanti

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
121 topic views. 4 members, 13 guests and 3 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 14 queries.