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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 777
Loc: PNW
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Koh Samui - Not pinning
#28608519 - 01/03/24 02:38 PM (1 year, 14 days ago) |
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I've got a 100% colonized unmodified mono of KSSS that I'm beginning to fear is stalling out.
I started from MSS on agar and performed 3 or 4 iterations isolating before using the agar to inoculate 3 quart jars of rye berry. It colonized both the agar plates and the rye very aggressively and appeared promising.
I spawned the jars to CVG at a 2:1 sub/spawn ratio with a 1/4" casing layer on 12/17. I allowed it to colonize for 8 days until it was around 80% before introducing fruiting conditions. Which basically was to just flip the lid and fan it. This was that day (12/28):

In the 2-3 days after fruiting, it filled out and looked fantastic. This is from 12/31:

Since then, there's been very little change really. I don't mist hardly at all (once a day maybe) and I fan with the cover 3-4 times a day for about 20 seconds. The temperature is consistently around 70°F and I bring it closer to the window during daylight hours. Something tells me that it's still not getting enough FAE due to how infrequently it needs misting. It's covered about 50% with these small bright-white thick knots that I assumed would sprout pins, but no go yet.

Thoughts/advice? Does Koh Samui have a reputation for fruiting slowly?
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Way
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
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Patience. It hasn't been long enough to think it has stalled or something is wrong.
The fact that you haven't needed to mist means your tub is fairly dialed in. You shouldn't have to be misting to keep surface conditions.
I suggest you stop fanning, it doesn't do anything and every time you open the tub you are messing up the microclimate. It's an old thing people used to do that they thought was useful and it really isn't.
Let it ride.
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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 777
Loc: PNW
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Re: Koh Samui - Not pinning [Re: Way]
#28609026 - 01/03/24 11:51 PM (1 year, 13 days ago) |
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Quote:
Patience. It hasn't been long enough to think it has stalled or something is wrong.
Yeah, you're probably right. Helicopter parent over here . Well, that and I've got another tub that I'm sub-consciously comparing it to, even though I know better (smaller, modified tub, with different sub-species)
Quote:
I suggest you stop fanning, it doesn't do anything and every time you open the tub you are messing up the microclimate. It's an old thing people used to do that they thought was useful and it really isn't.
But wait, it's an unmodified tub. Don't you need to fan to gas-off CO from the surface?
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Silentraindrops
mushlove student

Registered: 12/23/23
Posts: 223
Loc: pnw
Last seen: 10 months, 11 days
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Quote:
mycosispeon said:
Quote:
Patience. It hasn't been long enough to think it has stalled or something is wrong.
Yeah, you're probably right. Helicopter parent over here . Well, that and I've got another tub that I'm sub-consciously comparing it to, even though I know better (smaller, modified tub, with different sub-species)
Quote:
I suggest you stop fanning, it doesn't do anything and every time you open the tub you are messing up the microclimate. It's an old thing people used to do that they thought was useful and it really isn't.
But wait, it's an unmodified tub. Don't you need to fan to gas-off CO from the surface?
Enough gas exchange happens passively. Fanning is far to much in most cases. Oysters on the other hand.....
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starvinghooker
Voyager



Registered: 04/16/23
Posts: 540
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Yeah, fanning is not necessary. Just keep an eye on surface conditions and play the waiting game. If it just fully colonized 5 days ago, it could be another week or two before you see pins. Some can take even longer depending on genetics, temperature, and other factors.
Hoping the best for your grow!
-------------------- Grab life by the balls and yank on 'em til everything you want comes gushing out in thick, creamy ribbons
Noob like me? Start here!
Hitchhiker's Guide to the Shroomery
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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Hello and welcome to the forum! Good on you for taking the leap and learning agar for your first go!! I'm just going to echo everyone else and tell you to stop fanning. It is unnecessary and usually just leads to dried out substrates. As a beginner it is very easy to 'love your project to death'. I'll also say that unmodified tubs are inferior to PastyWhites EZ dial monotub. Once I started using it I never looked back.
Now for the proverbial raincloud on your picnic...my money is on mold. Sorry homie. I've been wrong before and I'm sure it will happen again, hopefully here, but I don't like the looks of those thick white patches. Notice how the unhealthy looking mycelial masses only appeared where the healthy looking mycelium wouldn't colonize the substrate? For me that's strikes 1 and 2.
Fingers crossed. I hope you prove me wrong!
Do you have any pictures of your agar plates or spawn from this tub?
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I 5318008
NOT a virgin!
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 777
Loc: PNW
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Quote:
bigfootscreepyuncl said: Hello and welcome to the forum! Good on you for taking the leap and learning agar for your first go!!
Thanks! It's actually not my first go, more like my first attempt at trying to do things the right way. I actually got into the hobby in early Apr 2022. Started with UB TEK originally and did that through the summer. I moved in Sept and decided to take a few steps to improve my TEK the biggest two being picking up a PC and building a proper SAB (a necessity for agar work).
Quote:
bigfootscreepyuncl said: I'm just going to echo everyone else and tell you to stop fanning. It is unnecessary and usually just leads to dried out substrates.
Okay, I'll put my faith in the mush gods and stop fanning.
Quote:
bigfootscreepyuncl said: I'll also say that unmodified tubs are inferior to PastyWhites EZ dial monotub. Once I started using it I never looked back.
I'll definitely check that out later. I've got a few smaller, modified tubs that I inherited, but I believe they were done incorrectly as the filter patches are all at the same level:

Quote:
bigfootscreepyuncl said: Do you have any pictures of your agar plates or spawn from this tub?
I don't have pix of the actual agar plates, but I did a transfer of each into three NEW dishes:
 KSSS D-090 Taken 12/12/23
 KSSS D-092 Taken 12/12/23
 KSSS D-093 Taken 12/12/23
Here are the three spawn jars the day I spawned to bulk:
 KSSS J-005-007 12 days after Noc; 5 day after BnS; right before spawning
This is my first post, but believe me, I've been lurking for months I'd been looking for information on reddit before I (thankfully) discovered this community.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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Quote:
mycosispeon said:
Quote:
bigfootscreepyuncl said: Hello and welcome to the forum! Good on you for taking the leap and learning agar for your first go!!
Thanks! It's actually not my first go, more like my first attempt at trying to do things the right way. I actually got into the hobby in early Apr 2022. Started with UB TEK originally and did that through the summer. I moved in Sept and decided to take a few steps to improve my TEK the biggest two being picking up a PC and building a proper SAB (a necessity for agar work).
Definitely made the right decisions on equipment. I still use a SAB for all my sterile work, simply because they work lol.
Quote:
bigfootscreepyuncl said: I'm just going to echo everyone else and tell you to stop fanning. It is unnecessary and usually just leads to dried out substrates.
Okay, I'll put my faith in the mush gods and stop fanning.
A properly dialed in monotub will have more than enough passive air flow (FAE or Fresh Air Exchange) by design, based on how humid air interacts with less humid air.
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bigfootscreepyuncl said: I'll also say that unmodified tubs are inferior to PastyWhites EZ dial monotub. Once I started using it I never looked back.
I'll definitely check that out later. I've got a few smaller, modified tubs that I inherited, but I believe they were done incorrectly as the filter patches are all at the same level:

I understand using what you have on hand, but I would suggest your next update be to the EZ dial tubs I linked above. Those monotubs with the giant holes and filter patches/polyfil are oldschool and very difficult to dial in surface conditions with. Second to clean spawn, surface conditions are king! Let the tub do all the work for you.
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bigfootscreepyuncl said: Do you have any pictures of your agar plates or spawn from this tub?
I don't have pix of the actual agar plates, but I did a transfer of each into three NEW dishes:
 KSSS D-090 Taken 12/12/23
That one individual 'fan' of rhizo growth is an indicator that it is running from a contaminant, likely a finely intertwined mold kinda 'hitch hiking' or 'piggybacking' your KSSS mycelium.
 KSSS D-092 Taken 12/12/23
Same as with the other plate. The distinct sectors indicate a contaminant meshed into the mushroom mycelium. On the top edge, from about 9-1 o'clock looks very suspicious, as does the small sector around 4 o'clock between the two rhizo sectors. Uneven growth like that is an indicator that the culture is running from something.
 KSSS D-093 Taken 12/12/23
this is your best looking plate by far but I still would have taken this another transfer or two to try and clean it and get it to be more uniform. Rhizo growth doesn't always mean clean culture!
Here are the three spawn jars the day I spawned to bulk:
 KSSS J-005-007 12 days after Noc; 5 day after BnS; right before spawning
Your grains look very wet. Oats? Whatever grain you use should be dry to the touch and should easily fall out of your hand without sticking and freely roll around in the jar (not stick to the jar). Unfortunately your jars also all look a little bacterial. An indicator of bacterial spawn is how some of the grains get smashed up against the glass and won't colonize (bottom portion of the left jar, for example). Another indicator of bacterial spawn is how thick and off-white your mycelium looks. If it ever appears creamy, thick or is pressing grains against the glass bacteria is a safe bet. Personally I am not a fan of the 'tiger drop'. IMO your hand spends too much time above the plate cutting it into pieces like that, then your hand spends too much time directly above an open grain jar waiting for the agar to fall in.
This is my first post, but believe me, I've been lurking for months I'd been looking for information on reddit before I (thankfully) discovered this community.
We're glad to have you! It's rare anymore to see a beginner who has actually done their homework, and you seem like the kind of person who will learn from mistakes and take constructive criticism. It looks like you are right on the edge of breaking through and being highly successful in this hobby. Dial in your grain prep and focus on your sterile technique when inoculating your grain jars. I drop one big wedge, like 1/4 plate into a jar and only ever hold my sterile scalpel over the jar for a split second. Keep up the good work, I'm excited to see how far you can take it!
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I 5318008
NOT a virgin!
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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Quote:
mycosispeon said: Here are the three spawn jars the day I spawned to bulk:
 KSSS J-005-007 12 days after Noc; 5 day after BnS; right before spawning
Hold on I missed this in my first response..For one, what is BnS? But more importantly, you spawned these jars just like that? The left two, maybe if I was just desperate to get a project going but the one on the right (shouldn't have been spawned in the first place) needed some more time.
When you're dealing with a stubborn jar like that you can shake/break it up and allow it to recolonize to cover all of the grains.
Since I'm on the topic, if your jars are difficult to break up then that is another indicator that something is off. It could just mean you have a lot of extra starches on the outside of the grains but it could mean bacteria. A clean, properly prepared jar should only take a few thumps on your palm to be completely broken up.
You're definitely on the right track though so don't take this as if I'm coming down on you just to rain on your parade!
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I 5318008
NOT a virgin!
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LadysKnight
Hello Ladies


Registered: 10/09/15
Posts: 2,331
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Excellent observations. Seconded.
-------------------- Don't follow leaders
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 777
Loc: PNW
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Quote:
bigfootscreepyuncl said: A properly dialed in monotub will have more than enough passive air flow (FAE or Fresh Air Exchange) by design, based on how humid air interacts with less humid air.
In other words, my unmodified tub is unlikely to be "dialed in" because it has no modifications, yeah?
Quote:
bigfootscreepyuncl said: Same as with the other plate. The distinct sectors indicate a contaminant meshed into the mushroom mycelium. On the top edge, from about 9-1 o'clock looks very suspicious, as does the small sector around 4 o'clock between the two rhizo sectors. Uneven growth like that is an indicator that the culture is running from something.
Couldn't the agar dishes simply be exhibiting multiple strains, some rhizomorphic and some tomentose? For example, couldn't the second dish (below) just be 5+ different strains of cubes, 3 of which are rhizomorphic?
 KSSS D-092 Taken 12/12/23
Quote:
bigfootscreepyuncl said: Your grains look very wet. Oats? Whatever grain you use should be dry to the touch and should easily fall out of your hand without sticking and freely roll around in the jar (not stick to the jar). Unfortunately your jars also all look a little bacterial. An indicator of bacterial spawn is how some of the grains get smashed up against the glass and won't colonize (bottom portion of the left jar, for example). Another indicator of bacterial spawn is how thick and off-white your mycelium looks. If it ever appears creamy, thick or is pressing grains against the glass bacteria is a safe bet. Personally I am not a fan of the 'tiger drop'. IMO your hand spends too much time above the plate cutting it into pieces like that, then your hand spends too much time directly above an open grain jar waiting for the agar to fall in.
It's rye berry and I used SpitballJedi's write up to prepare it. It was dry (tested using toilet paper) so I don't suspect the grain prep (plus grains from the same iteration were used to colonize and then spawn my other tub which is currently fruiting nicely), so it would have either been the culture itself or the 'tiger drop' like you said. I also did a BnS which I think was a mistake. Well, and also I had the jars in my incubation box for a few days and the temperature got up to 82°F for a day before I caught it.
Quote:
bigfootscreepyuncl said: We're glad to have you! It's rare anymore to see a beginner who has actually done their homework, and you seem like the kind of person who will learn from mistakes and take constructive criticism. It looks like you are right on the edge of breaking through and being highly successful in this hobby. Dial in your grain prep and focus on your sterile technique when inoculating your grain jars. I drop one big wedge, like 1/4 plate into a jar and only ever hold my sterile scalpel over the jar for a split second. Keep up the good work, I'm excited to see how far you can take it!
I can't tell you how much I appreciate you taking the time to reply and help me course correct. With all of the information out there (even just on Shroomery) it's easy to get overwhelmed. I stopped referring to sub-Reddits a few months ago. I've spent a ton of time reading on the non-forum side of the site, so I get all of my information here and in a few books I've picked up (Stamet's "The Mushroom Cultivator" being a big one).
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 777
Loc: PNW
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Quote:
bigfootscreepyuncl said:
Quote:
mycosispeon said: Here are the three spawn jars the day I spawned to bulk:
 KSSS J-005-007 12 days after Noc; 5 day after BnS; right before spawning
Hold on I missed this in my first response..For one, what is BnS? But more importantly, you spawned these jars just like that? The left two, maybe if I was just desperate to get a project going but the one on the right (shouldn't have been spawned in the first place) needed some more time.
When you're dealing with a stubborn jar like that you can shake/break it up and allow it to recolonize to cover all of the grains.
Since I'm on the topic, if your jars are difficult to break up then that is another indicator that something is off. It could just mean you have a lot of extra starches on the outside of the grains but it could mean bacteria. A clean, properly prepared jar should only take a few thumps on your palm to be completely broken up.
You're definitely on the right track though so don't take this as if I'm coming down on you just to rain on your parade!
"BnS" == Break and Shake. Yeah, you're probably right on that... I probably pulled the trigger too soon. At this point, I'm thinking my most likely point of failure was the 'tiger drop'. Otherwise, the culture itself.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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Quote:
mycosispeon said:
Quote:
bigfootscreepyuncl said: A properly dialed in monotub will have more than enough passive air flow (FAE or Fresh Air Exchange) by design, based on how humid air interacts with less humid air.
In other words, my unmodified tub is unlikely to be "dialed in" because it has no modifications, yeah?
Correct. Unmodified tubs are good at retaining humidity, which is good, but a slow controlled evaporation is a major pinning trigger and unmodified tubs aren't as good at providing FAE. Unmodified tubs can and do work, but in my experience an EZ dial monotub outperforms an unmodified tub. When I spawn a (ez dial) tub I latch the lid closed and open it to harvest. Truly set and forget.
Quote:
bigfootscreepyuncl said: Same as with the other plate. The distinct sectors indicate a contaminant meshed into the mushroom mycelium. On the top edge, from about 9-1 o'clock looks very suspicious, as does the small sector around 4 o'clock between the two rhizo sectors. Uneven growth like that is an indicator that the culture is running from something.
Couldn't the agar dishes simply be exhibiting multiple strains, some rhizomorphic and some tomentose? For example, couldn't the second dish (below) just be 5+ different strains of cubes, 3 of which are rhizomorphic?
I'm not saying that's impossible or even that it's not the case, I'm just saying it's improbable and more often than not when you have severely sectored plates like you have the end result is usually lacking luster as a best case scenario. Uniform growth, regardless of rhizomorphic or tomentose is something you should strive for. In the future if you encounter this again run an experiment - take a transfer from the tomentose and the rhizomorphic sectors and run them each independently. Science! Keep in mind that on your plates there are 1,000s of 'strains'. Again, I'm not saying you're wrong. All I'm saying is that many people have been in similar situations because they ran sectored plates.
 KSSS D-092 Taken 12/12/23
Quote:
bigfootscreepyuncl said: Your grains look very wet. Oats? Whatever grain you use should be dry to the touch and should easily fall out of your hand without sticking and freely roll around in the jar (not stick to the jar). Unfortunately your jars also all look a little bacterial. An indicator of bacterial spawn is how some of the grains get smashed up against the glass and won't colonize (bottom portion of the left jar, for example). Another indicator of bacterial spawn is how thick and off-white your mycelium looks. If it ever appears creamy, thick or is pressing grains against the glass bacteria is a safe bet. Personally I am not a fan of the 'tiger drop'. IMO your hand spends too much time above the plate cutting it into pieces like that, then your hand spends too much time directly above an open grain jar waiting for the agar to fall in.
It's rye berry and I used SpitballJedi's write up to prepare it. It was dry (tested using toilet paper) so I don't suspect the grain prep (plus grains from the same iteration were used to colonize and then spawn my other tub which is currently fruiting nicely), so it would have either been the culture itself or the 'tiger drop' like you said. I also did a BnS which I think was a mistake. Well, and also I had the jars in my incubation box for a few days and the temperature got up to 82°F for a day before I caught it.
Breaking up spawn isn't a mistake, it's actually recommended. I will typically bust mine up around 30% colonization and sometimes again at about 80%. The speed at which your mycelium recovers from BnS will tell you a lot about how clean your spawn is. If I shake at 80% I want my entire jar to be recovered and 'fuzzy' again within 24 hours. It's just an indicator of how healthy your mycelium is.
Unless your house temperature is in the low 60s I would also recommend you ditch the incubation chamber. Cubensis do just fine anywhere from 65-80, they're just a little slower at the lower temperatures. More often than not incubation chambers create a better environment for contaminants than for mushroom mycelium. By increasing the temperatures you're challenging molds and bacteria to a drag race but you're giving them a Porsche and you're in a Geo Metro lol.
Quote:
bigfootscreepyuncl said: We're glad to have you! It's rare anymore to see a beginner who has actually done their homework, and you seem like the kind of person who will learn from mistakes and take constructive criticism. It looks like you are right on the edge of breaking through and being highly successful in this hobby. Dial in your grain prep and focus on your sterile technique when inoculating your grain jars. I drop one big wedge, like 1/4 plate into a jar and only ever hold my sterile scalpel over the jar for a split second. Keep up the good work, I'm excited to see how far you can take it!
I can't tell you how much I appreciate you taking the time to reply and help me course correct. With all of the information out there (even just on Shroomery) it's easy to get overwhelmed. I stopped referring to sub-Reddits a few months ago. I've spent a ton of time reading on the non-forum side of the site, so I get all of my information here and in a few books I've picked up (Stamet's "The Mushroom Cultivator" being a big one).
I've never been on Reddit but I've seen many links posted and it makes me want to pull my hair out..whatevers left of it lol. There is certainly a plethora of information on this forum - it's old, a little outdated and can be tricky to navigate at time but it's archived back to the 90's. Another good thing about this forum is that while it may not seem like it is, this is a very 'scholarly' forum. Methods are theorized, tested, repeated, and if they're valid they get adopted by the community and improved on over time (if needed). For this reason it is advised to use trusted and up-to-date TEKs. If you use the search function on this forum you can filter and refine your searches by whether or not it was written by a TC (Trusted Cultivator) and how recent you want it to have been 'published'. I usually do a 'key word' search, select for it to have been written by a TC and I don't usually look further back than 3 years. If there is something you want to know about, can't find something written about it and nobody can answer your question then you could write likely write a PhD dissertation on it and have it published.
Sorry for my long-winded responses, but I'm happy to help as are most of the other members on here.
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I 5318008
NOT a virgin!
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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If you'd like to have some fun, make some friends in the community, learn some things and have a group of people more than willing to help you come on over and join LAGM! Lets All Grow Mushrooms is an annual tradition on the shroomery that was started many years ago to show beginners and seasoned cultivators alike how easy agar is. In a nut shell it is one giant community grow-along. Each participant creates their own grow log and documents their process from start to finish. Every year it starts on New Years Day so you're only a few days behind.
It really is a lot of fun and a great way to get questions answered (usually faster and in more detail than if you're just posting on the main forum). I'm in there, a handful of TCs, many other seasoned cultivators and a lot of beginners as well. It's just a great way to learn, meet some people and show off a little while you're at it. Plus, once everyone starts harvesting there's usually a lot of sharing of spore prints/swabs.
The only requirement is that your grow starts from spores, on agar. That's it! Hop on over and feel free to join if it sounds like a good time to you. And if you need some new spores to get started on LAGM shoot me a message and I'll get you taken care of
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I 5318008
NOT a virgin!
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 777
Loc: PNW
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Quote:
bigfootscreepyuncl said: Breaking up spawn isn't a mistake, it's actually recommended. I will typically bust mine up around 30% colonization and sometimes again at about 80%. The speed at which your mycelium recovers from BnS will tell you a lot about how clean your spawn is. If I shake at 80% I want my entire jar to be recovered and 'fuzzy' again within 24 hours. It's just an indicator of how healthy your mycelium is.
Unless your house temperature is in the low 60s I would also recommend you ditch the incubation chamber. Cubensis do just fine anywhere from 65-80, they're just a little slower at the lower temperatures. More often than not incubation chambers create a better environment for contaminants than for mushroom mycelium. By increasing the temperatures you're challenging molds and bacteria to a drag race but you're giving them a Porsche and you're in a Geo Metro lol.
You don't even incubate your agar dishes?
Alsi, thanks for the search tips
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mycosispeon
Goofy's bastard child


Registered: 05/17/23
Posts: 777
Loc: PNW
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Quote:
bigfootscreepyuncl said: If you'd like to have some fun, make some friends in the community, learn some things and have a group of people more than willing to help you come on over and join LAGM! Lets All Grow Mushrooms is an annual tradition on the shroomery that was started many years ago to show beginners and seasoned cultivators alike how easy agar is. In a nut shell it is one giant community grow-along. Each participant creates their own grow log and documents their process from start to finish. Every year it starts on New Years Day so you're only a few days behind.
It really is a lot of fun and a great way to get questions answered (usually faster and in more detail than if you're just posting on the main forum). I'm in there, a handful of TCs, many other seasoned cultivators and a lot of beginners as well. It's just a great way to learn, meet some people and show off a little while you're at it. Plus, once everyone starts harvesting there's usually a lot of sharing of spore prints/swabs.
The only requirement is that your grow starts from spores, on agar. That's it! Hop on over and feel free to join if it sounds like a good time to you. And if you need some new spores to get started on LAGM shoot me a message and I'll get you taken care of 
Hell yes! That's totally up my alley. Unfortunately the only genetics I have to work with are MSS syringes and I'm beginning to think a few are suspect. I'll hit you up.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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Quote:
mycosispeon said:
Quote:
bigfootscreepyuncl said: Breaking up spawn isn't a mistake, it's actually recommended. I will typically bust mine up around 30% colonization and sometimes again at about 80%. The speed at which your mycelium recovers from BnS will tell you a lot about how clean your spawn is. If I shake at 80% I want my entire jar to be recovered and 'fuzzy' again within 24 hours. It's just an indicator of how healthy your mycelium is.
Unless your house temperature is in the low 60s I would also recommend you ditch the incubation chamber. Cubensis do just fine anywhere from 65-80, they're just a little slower at the lower temperatures. More often than not incubation chambers create a better environment for contaminants than for mushroom mycelium. By increasing the temperatures you're challenging molds and bacteria to a drag race but you're giving them a Porsche and you're in a Geo Metro lol.
You don't even incubate your agar dishes?
Alsi, thanks for the search tips
The only thing I ever 'incubate' or apply heat to is my veggie seeds. My agar plates literally just sit in my desk drawer at room temp (~72F). They don't even get light unless I'm looking at them lol.
Happy to help. See you around LAGM
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I 5318008
NOT a virgin!
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 777
Loc: PNW
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Quote:
Way said: Patience. It hasn't been long enough to think it has stalled or something is wrong.
You were right. It started pinning yesterday. There's over a hundred brown little babies coming to life.
 T-008 on 01/06/24; 20 days after NOC; 9 days in FC
Quote:
bigfootscreepyuncl said: Now for the proverbial raincloud on your picnic...my money is on mold.
You could still be right, but by god I hope you're wrong.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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Quote:
mycosispeon said:
Quote:
Way said: Patience. It hasn't been long enough to think it has stalled or something is wrong.
You were right. It started pinning yesterday. There's over a hundred brown little babies coming to life.
 T-008 on 01/06/24; 20 days after NOC; 9 days in FC
Quote:
bigfootscreepyuncl said: Now for the proverbial raincloud on your picnic...my money is on mold.
You could still be right, but by god I hope you're wrong. 

I hope I'm wrong to! I've been waiting for the update lol...I hope that thing goes 4 flushes and I have to eat every one of my words publicly
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I 5318008
NOT a virgin!
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Silentraindrops
mushlove student

Registered: 12/23/23
Posts: 223
Loc: pnw
Last seen: 10 months, 11 days
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Quote:
bigfootscreepyuncl said:
Quote:
mycosispeon said:
Quote:
Way said: Patience. It hasn't been long enough to think it has stalled or something is wrong.
You were right. It started pinning yesterday. There's over a hundred brown little babies coming to life.
 T-008 on 01/06/24; 20 days after NOC; 9 days in FC
Quote:
bigfootscreepyuncl said: Now for the proverbial raincloud on your picnic...my money is on mold.
You could still be right, but by god I hope you're wrong. 

I hope I'm wrong to! I've been waiting for the update lol...I hope that thing goes 4 flushes and I have to eat every one of my words publicly
GRATS, agreed make him eat his dirty words.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,736
Loc: Gamehenge
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Quote:
Silentraindrops said: GRATS, agreed make him eat his dirty words.
I'm never afraid or ashamed to admit when I'm wrong. That's how you learn. It appears I was wrong about the mold (which is a good thing, after all). Hopefully, despite my incorrect diagnosis, I was otherwise helpful. My concern of mold wasn't unfounded though based on several other pieces of information throughout the post, but also from my own experience.
Every time I've encountered trichoderma in my own tubs it first appears as dense, irregular patches of off-white mycelium, similar to the mycelial masses OP shared in his first post.
can you spot the mold outbreak? It looks fairly similar in my opinion.
how about in this one? 
My suspicion wasn't unfounded, but for OP's sake I am very glad that I was incorrect this time (for now ).
Words. Eaten.
Bigfoot
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I 5318008
NOT a virgin!
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 777
Loc: PNW
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Shhheeeaaaattt... There's still plenty of time for shit to go tits up here. To be continued...
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 777
Loc: PNW
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Thought I'd post an update here.
It's still truckin' along
 T-008 taken 01/09/24
However, Bigfoot I think you are right in that there are signs of bacterial contamination (browning on stipes, some malformed fruits)
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 777
Loc: PNW
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Well this is strange (or actually not considering my spawn was clearly not healthy)... a few of the fruits have partially torn veils, but are only about 1 1/2" tall.
 T-008 taken 01/13/24
This must be a result of bacterial contamination similar to my tub of AA+, right?
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 777
Loc: PNW
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Re: Koh Samui - Not pinning [Re: mycosispeon] 1
#28627745 - 01/19/24 08:16 PM (11 months, 22 days ago) |
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An update on this tub: It's largely been a disappointment. None of the fruits grew to > 1.5" tall.

The last 2-3 days, it appears to have stalled out, so I flushed out the remaining fruits that were big enough to dry. Somehow I still ended up with 228g wet. Had a shit ton of aborts though... I'd probably say 75% ish.
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mycosispeon
Goofy's bastard child

Registered: 05/17/23
Posts: 777
Loc: PNW
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Re: Koh Samui - Not pinning [Re: mycosispeon]
#28674434 - 02/24/24 04:42 PM (10 months, 17 days ago) |
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Recording for posterity 
I decided to ditch this grow after the second flush. 3 jars spawned at a 1:2, 51 days from spawning to disposal. Total dry weight: 36.7g.
While the grow was disappointing, I finally got around to trying these fruits out last night, and had an amazing time. 5.05g ground and lemon TEKed, was intense, cerebral, and lasted around 5.5-6 hours.
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