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3.A.M
Boop!



Registered: 10/17/22
Posts: 848
Loc: Oz
Last seen: 4 hours, 12 minutes
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Literally did that today (: took a swab though and am giving it a week or two to dry out before putting to agar, considering it’s an F2 JF/PE cross I’ll be guaranteed to know if it’s a clone or spores though (unless I’m missing something in my reasoning, it’s late here and I’ve got a migraine) so if anyone has any thoughts or information that’ll shoot this down before I potentially waste my time I’d be happy to hear it. Only issue I’ve had with it so far is it’s fairly useless with smaller varieties or those with smaller gills.
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San Pedro Girl
Shoebox Ninja🥷




Registered: 07/17/12
Posts: 2,385
Loc: Fuck off pig!🐷
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Re: Cultivation General Discussion [Re: 3.A.M]
#28605671 - 01/01/24 10:49 AM (26 days, 20 hours ago) |
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One thing I discovered today and wanted to share while playing with pre poured agar plates vs my usual poured in the hood…
I get the same level of sterilization with my 100mm/≈17ml plates in the PC for 24min as I did with my 400ml media bottle in there for 36min. Time and energy saver if anyone cares.
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RockinRobot
Stranger


Registered: 12/08/22
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Quote:
San Pedro Girl said: One thing I discovered today and wanted to share while playing with pre poured agar plates vs my usual poured in the hood…
I get the same level of sterilization with my 100mm/≈17ml plates in the PC for 24min as I did with my 400ml media bottle in there for 36min. Time and energy saver if anyone cares.
IMO saving 10 min isn't worth the difference. I would still let it go your original time. I always run agar 30min regardless of quantity. Same with LC.
You can't really over sterilize something.
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San Pedro Girl
Shoebox Ninja🥷




Registered: 07/17/12
Posts: 2,385
Loc: Fuck off pig!🐷
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Re: Cultivation General Discussion [Re: RockinRobot]
#28605692 - 01/01/24 11:07 AM (26 days, 20 hours ago) |
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Quote:
RockinRobot said:
Quote:
San Pedro Girl said: One thing I discovered today and wanted to share while playing with pre poured agar plates vs my usual poured in the hood…
I get the same level of sterilization with my 100mm/≈17ml plates in the PC for 24min as I did with my 400ml media bottle in there for 36min. Time and energy saver if anyone cares.
IMO saving 10 min isn't worth the difference. I would still let it go your original time. I always run agar 30min regardless of quantity. Same with LC.
You can't really over sterilize something.
The best way to sterilize your agar is to calculate your time based on volume. For example, my plates are more sterile at 24min than your bottle of agar is at 30min. And my 400ml media bottle is more sterile at 36min than your 400ml bottle is at 30min. Once you hit a certain factor, it’s pointless to keep going. It’s just a waste of energy and time.
If you want to sterilize all your agar for the same time, without worrying about volume/time/energy, I would definitely suggest increasing from 30min to 45min.
This may be interesting for you.🙂 https://www.shroomery.org/forums/showflat.php/Number/28166056#28166056
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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has anyone else vacuum sealed their dry mushrooms but looked 3-4 days later and see that the bag now has more air in it, even though the seal is still good?
i've tried sucking out all the air from the bag using a vacuum sealer. i've sealed it with multiple wide seals using a very nice impulse sealer. i've even tried using an oxygen absorber. none of it helps. after 3-4 days, the bag still takes on air. but the seal is not bad. i can submerge the bag underwater and even squeeze it but it does not leak.
so what's producing the air? the only thing i can think of is some kind of microbe. but they've been dried for 24+ hours in a dehydrator at 160F so everything should be dead. wtf mate
-------------------- (Yes, the egg is real)
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A.k.a
Stranger



Registered: 10/27/19
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Re: Cultivation General Discussion [Re: FriedEgg] 1
#28607450 - 01/02/24 05:22 PM (25 days, 14 hours ago) |
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Yep I’m pretty sure mine was from stiff shrooms poking micro holes into the bag as they got crushed.
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LAGM2020     
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tryptkaloids
Learner



Registered: 02/08/15
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Re: Cultivation General Discussion [Re: FriedEgg] 3
#28607452 - 01/02/24 05:23 PM (25 days, 14 hours ago) |
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Those bags aren't air tight. Different bags have different permeability, but others have reported the same issue.
In a sealed jar is the preferred method these days
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Cultivation General Discussion [Re: tryptkaloids]
#28607460 - 01/02/24 05:29 PM (25 days, 14 hours ago) |
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Quote:
A.k.a said: Yep I’m pretty sure mine was from stiff shrooms poking micro holes into the bag as they got crushed.
Quote:
tryptkaloids said: Those bags aren't air tight. Different bags have different permeability, but others have reported the same issue.
In a sealed jar is the preferred method these days
if these vacuum sealer bags are not air tight, why won't water leak into the bag if i dunk it and squeeze or massage the bag underwater?
i'm going to do a test. i'm going to vacuum seal some mushrooms, dunk the bag underwater and keep it there underwater for 3-5 days and see what it looks like then. if it still puffs up with air then it has to be something inside the bag creating gas.
-------------------- (Yes, the egg is real)
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RockinRobot
Stranger


Registered: 12/08/22
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Re: Cultivation General Discussion [Re: FriedEgg] 6
#28607482 - 01/02/24 05:44 PM (25 days, 13 hours ago) |
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Quote:
FriedEgg said:
Quote:
A.k.a said: Yep I’m pretty sure mine was from stiff shrooms poking micro holes into the bag as they got crushed.
Quote:
tryptkaloids said: Those bags aren't air tight. Different bags have different permeability, but others have reported the same issue.
In a sealed jar is the preferred method these days
if these vacuum sealer bags are not air tight, why won't water leak into the bag if i dunk it and squeeze or massage the bag underwater?
i'm going to do a test. i'm going to vacuum seal some mushrooms, dunk the bag underwater and keep it there underwater for 3-5 days and see what it looks like then. if it still puffs up with air then it has to be something inside the bag creating gas.
Air permeable and water permeable are not the same.
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YoshiTrainer
Onion tied to belt



Registered: 04/30/22
Posts: 1,202
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Re: Cultivation General Discussion [Re: RockinRobot] 3
#28607503 - 01/02/24 06:02 PM (25 days, 13 hours ago) |
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I vacuum seal bags with a moisture absorbing pack inside. I put the moisture absorbing pack in the dehydrator for the last 1/2 hour of the mushroom run. Seemes to work fine for me!
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Way
The


Registered: 01/14/23
Posts: 4,336
Loc: A long way away
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Re: Cultivation General Discussion [Re: YoshiTrainer]
#28607534 - 01/02/24 06:24 PM (25 days, 13 hours ago) |
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Over a long enough period of time, air will slowly leak back into vacuum bags. It may not be noticeable in a shorter period, but anyone who has stored food in vacuum bags has likely seen it.
As stated, different bags let air in at different rates.
If you want it to be completely air tight, you have to use glass.
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That's the way she goes, boys. Sometimes she goes, sometimes she doesn't, cause that's the fuckin way she goes.
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CaptainPuffy
Marbles Lost


Registered: 06/08/11
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Re: Cultivation General Discussion [Re: Way]
#28607581 - 01/02/24 06:55 PM (25 days, 12 hours ago) |
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I live in climate zone 4a. I'm considering doing an experiment to attempt to grow woodlovers outdoors. I'm thinking of using milk crates so they can be stacked and move them inside before the freeze. It's my hope that milk crates will provide extra fae and easy watering.
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San Pedro Girl
Shoebox Ninja🥷




Registered: 07/17/12
Posts: 2,385
Loc: Fuck off pig!🐷
Last seen: 13 hours, 4 seconds
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Re: Cultivation General Discussion [Re: RockinRobot]
#28607590 - 01/02/24 06:59 PM (25 days, 12 hours ago) |
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Quote:
RockinRobot said:
Quote:
FriedEgg said:
Quote:
A.k.a said: Yep I’m pretty sure mine was from stiff shrooms poking micro holes into the bag as they got crushed.
Quote:
tryptkaloids said: Those bags aren't air tight. Different bags have different permeability, but others have reported the same issue.
In a sealed jar is the preferred method these days
if these vacuum sealer bags are not air tight, why won't water leak into the bag if i dunk it and squeeze or massage the bag underwater?
i'm going to do a test. i'm going to vacuum seal some mushrooms, dunk the bag underwater and keep it there underwater for 3-5 days and see what it looks like then. if it still puffs up with air then it has to be something inside the bag creating gas.
Air permeable and water permeable are not the same.
 Exactly, think about how a bicycle inner tube is water tight but still leaks air over time. Extreme example, but illustrates the point. That’s why zip lock bags or shrink wrap work great on plates. They still exchange gasses.
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xVadisx
MountainsDogsAndFungi



Registered: 09/03/21
Posts: 1,122
Loc: Secret World HQ
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Glass is the way to go. Properly dried mushrooms into glass with desiccant and then vacuum sealed. Even this isn't perfect. The seal can break, etc.
Best method is to eat them. Or find friends to eat them.
-------------------- Thanks to everyone for sharing what they know . 165F for 24hrs. Better Holes Tek (Use a Step Drill Bit) Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY
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FriedEgg



Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Quote:
San Pedro Girl said:
Quote:
RockinRobot said:
Quote:
FriedEgg said:
Quote:
A.k.a said: Yep I’m pretty sure mine was from stiff shrooms poking micro holes into the bag as they got crushed.
Quote:
tryptkaloids said: Those bags aren't air tight. Different bags have different permeability, but others have reported the same issue.
In a sealed jar is the preferred method these days
if these vacuum sealer bags are not air tight, why won't water leak into the bag if i dunk it and squeeze or massage the bag underwater?
i'm going to do a test. i'm going to vacuum seal some mushrooms, dunk the bag underwater and keep it there underwater for 3-5 days and see what it looks like then. if it still puffs up with air then it has to be something inside the bag creating gas.
Air permeable and water permeable are not the same.
 Exactly, think about how a bicycle inner tube is water tight but still leaks air over time. Extreme example, but illustrates the point. That’s why zip lock bags or shrink wrap work great on plates. They still exchange gasses.
ok that's a really good point with the tire example
but this is not a really long time. this is a few days. it's just really odd. i wish there was a better way.
-------------------- (Yes, the egg is real)
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3.A.M
Boop!



Registered: 10/17/22
Posts: 848
Loc: Oz
Last seen: 4 hours, 12 minutes
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Re: Cultivation General Discussion [Re: FriedEgg] 1
#28607681 - 01/02/24 08:27 PM (25 days, 11 hours ago) |
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I’ve gotten so sick of my vac seal bags letting air in I’ve invested in some food grade buckets, will just keep rotating silica every month or so, glass jars don’t stack so well, are more expensive and don’t come large enough for my needs unfortunately.
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tedoro
ToadStool Tender



Registered: 02/06/15
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Re: Cultivation General Discussion [Re: 3.A.M]
#28607728 - 01/02/24 09:06 PM (25 days, 10 hours ago) |
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The company 'outofair' sells thicker rolls of impulse bags... I double seal each end and find the pokey shrooms cant pop that brands bags. My bags are tight for a long old time (easily a year). 5% might relax in time, I just redo them.
-------------------- -------------------- Deep pour soft agar plates-->bags of WBS-->Low Profile Monos Clean spawn thread | Put a thermometer on your PC
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A.k.a
Stranger



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Re: Cultivation General Discussion [Re: tedoro]
#28607970 - 01/03/24 04:19 AM (25 days, 3 hours ago) |
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I should add that 90% of my vacuum sealed ounces have stayed sealed for 6-12 months and counting.
The ones that let air in seemed to be the super dense shrooms so I assume they were just sturdy enough to mess up the bag instead of compacting slightly like normal.
I do keep my best stuff in jars though. Kinda bums me out when I cut open a vacuum bag and the shrooms crumble from being crushed.
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LAGM2020     
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Mr Piggy
Big Dick Retard



Registered: 09/29/11
Posts: 8,379
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Quote:
San Pedro Girl said:
Quote:
RockinRobot said:
Quote:
FriedEgg said:
Quote:
A.k.a said: Yep I’m pretty sure mine was from stiff shrooms poking micro holes into the bag as they got crushed.
Quote:
tryptkaloids said: Those bags aren't air tight. Different bags have different permeability, but others have reported the same issue.
In a sealed jar is the preferred method these days
if these vacuum sealer bags are not air tight, why won't water leak into the bag if i dunk it and squeeze or massage the bag underwater?
i'm going to do a test. i'm going to vacuum seal some mushrooms, dunk the bag underwater and keep it there underwater for 3-5 days and see what it looks like then. if it still puffs up with air then it has to be something inside the bag creating gas.
Air permeable and water permeable are not the same.
 Exactly, think about how a bicycle inner tube is water tight but still leaks air over time. Extreme example, but illustrates the point. That’s why zip lock bags or shrink wrap work great on plates. They still exchange gasses.
And as a person who has done a number of tube repairs, spraying with soapy water or holding it underwater to see where the air is leaking from is a totally valid way of using submersion to locate a perforation.
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RockinRobot
Stranger


Registered: 12/08/22
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Re: Cultivation General Discussion [Re: Mr Piggy] 1
#28608214 - 01/03/24 09:34 AM (24 days, 22 hours ago) |
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With an innertube you are working with a puncture not permeability. Innertubes will in fact also lose air over time due to permeability which you will NOT be able to see by holding it underwater and looking for bubbles.
Edited by RockinRobot (01/03/24 09:35 AM)
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