Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1
OfflineKinichi
Stranger
Male

Registered: 12/13/23
Posts: 9
Loc: Midwest
Last seen: 11 days, 58 minutes
Concentrating Liquid Myc
    #28607164 - 01/02/24 02:04 PM (25 days, 7 hours ago)

I have searched the posts and could not find anything that takes into account the concentration level of mycelium in a liquid cultures. There are many great recipes and techniques here, but there comes a point when any LC will slow or stop expanding in the media its growing. At this point there is a portion of the nutrient mixture that is not going to be consumed. This becomes the separator solution when shaking/mixing the LC.

MY THOUGHTS or QUESTIONS:
1. Some of the media will already be infused into the myco blob that is growing - IS ALL OF THE REMAINING NECESSARY OR BENEFICIAL TO INOCULATION?

2. If you pull out some of the unused media prior to shaking/mixing -
    WILL THE MYC STILL SEPARATE ENOUGH TO PULL THROUGH AN 18 GA SYRINGE?
    WILL THE INCREASED CONCENTRATION SPEED UP COLONIZATION?

My suspicion is that having a greater concentration of myco that can pass through the syringe might accomplish a couple things.

A. The nutrient solution will be used faster and the mycelium will search out new nutrient sources (Bulk Spawn or Cakes or whatever is being used).

B. The decrease in moisture added to the bulk could help reduce opportunistic contam that was waiting for a little more water to start growing. 

C. It might decrease total colonization time for the Bulk or Cake.

I am getting ready to run an experiment that will test this theory in a few ways over a few different Bulk set ups with some LC that i have that has just about stopped expanding. If you have any experience with this theory or any recomendations for possible vectors to address let me know.


Extras: Unfilter Print Post Top
OfflineHelloImBob
Old Guy
I'm a teapot
Registered: 03/30/08
Posts: 219
Last seen: 1 hour, 31 minutes
Re: Concentrating Liquid Myc [Re: Kinichi]
    #28620756 - 01/14/24 10:03 AM (13 days, 11 hours ago)

If your trying to experiment why wouldn't you start with the easiest possible method?

Get yourself some larger gauge needles such as 14ga for LC and before you shake your jar remove as much of the liquid as you can.

It may help in the future to get replies if your posts in advanced mycology sound like it's worth replying to. This isn't anything goes general cultivation and the people who would reply expect you to have a certain level of intelligence for it to be worth their time.

Sorry not trying to be rude or mean that's just how it is.


--------------------
Quote from Stipe-n-Cap

"You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."


Extras: Unfilter Print Post Top
OfflineKinichi
Stranger
Male

Registered: 12/13/23
Posts: 9
Loc: Midwest
Last seen: 11 days, 58 minutes
Re: Concentrating Liquid Myc [Re: HelloImBob]
    #28622599 - 01/15/24 07:35 PM (12 days, 1 hour ago)

No worries mate.

The forums used to be a place where you could bring up questions or theories about different approaches and discuss their possibilities. Definitely not that way anymore.

"If your trying to experiment why wouldn't you start with the easiest possible method?" 

Which method would that be?



All good


Extras: Unfilter Print Post Top
OfflineHelloImBob
Old Guy
I'm a teapot
Registered: 03/30/08
Posts: 219
Last seen: 1 hour, 31 minutes
Re: Concentrating Liquid Myc [Re: Kinichi]
    #28624652 - 01/17/24 02:21 PM (10 days, 7 hours ago)

Get yourself some larger gauge needles such as 14ga for LC and before you shake your jar remove as much of the liquid as you can. (Possible contam issues if not done correctly)

Or let the mycelium settle to the bottom of your sryinge and push out the extra liquid before using and repeat

Personally I don't see much benefit to concentrating since you can colonize way faster than you can fruit already with LC unless I'm missing something

4-7 days for a quart seems fast enough


--------------------
Quote from Stipe-n-Cap

"You appear to be talking about boosting tryptamine content in mycelium by amending LC with....whatever your amendments are. I have to say I'm a tad disappointed that you're addressing us with the shorthand of a 13 yo girl who's texting her besty for make-up tips, instead of proper English, which causes me to have doubts."


Extras: Unfilter Print Post Top
Jump to top Pages: 1

Shop: Kraken Kratom Red Vein Kratom   North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Malt to water ratio for liquid myc? Pugslee_Atoms420 782 4 03/27/03 06:32 AM
by Pugslee_Atoms420
* Liquid Myc Culture Recipie Alger 2,430 13 01/07/03 10:23 AM
by whiterasta
* Does anyone use brown sugar as liquid media? Why not? forgieboy 2,958 14 09/05/04 03:46 PM
by Mycena
* LIquid culture to dehydrater??? ruderallis 2,450 14 10/12/02 07:21 PM
by ruderallis
* Liquid Mycelium Culturing Erawk 2,094 9 02/12/02 07:06 AM
by kevman
* High Volume One Pot Liquid Tek? optigram 1,560 12 11/27/03 08:22 AM
by micro
* Re: Glucose liquid Myc. IT WORKED!! Anonymous 1,179 1 01/20/00 04:21 AM
by Anonymous
* Growing in Liquid Medium. Using knowledge from Reactor Des
( 1 2 3 all )
Randoscious_McCray 15,673 51 03/19/05 12:56 AM
by Randoscious_McCray

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
208 topic views. 1 members, 2 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Date | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.017 seconds spending 0.004 seconds on 12 queries.