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OfflineCassimere
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Registered: 12/15/23
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Harvesting Mycellium
    #28598145 - 12/25/23 11:50 PM (1 month, 1 day ago)

I have a few bags of lion's mane ready to pop, but does anyone know how to harvest the mycellium? I have some on rice, and some on wheat.


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OfflineSilentraindrops
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Re: Harvesting Mycellium [Re: Cassimere]
    #28598176 - 12/26/23 12:25 AM (1 month, 1 day ago)

You probably won't find much info here about lion's mane it's considered a gourmet mushroom, there's another section on this site for .... also there are other websites that detail professionals - noob level lions mane. If you are looking to go from bag of mycelium to full grown lions mane i suggest youtube videos for the next stage and what's required. I just don't know this section of the forums is mainly for cubensis mushrooms . with some other exotics as well . but mainly cube.

are you trying to get the mycelium for agar?


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Edited by Silentraindrops (12/26/23 12:25 AM)


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OfflineLogicaL ChaosM
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Re: Harvesting Mycellium (moved) [Re: Cassimere]
    #28598223 - 12/26/23 02:44 AM (1 month, 1 day ago)

This thread was moved from Mushroom Cultivation.

Reason:
Proper forum here.


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OfflineStromriderM
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Re: Harvesting Mycellium (moved) [Re: LogicaL Chaos] * 1
    #28598296 - 12/26/23 06:22 AM (1 month, 1 day ago)

Why would you want to harvest the mycelium?

Mushrooms are made of mycelium, just harvest mushrooms


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InvisibleSonOfAHooker
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Registered: 03/11/19
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Re: Harvesting Mycellium (moved) [Re: Stromrider]
    #28598623 - 12/26/23 01:49 PM (1 month, 1 day ago)

I'd guess it's to ingest for the medicinal benefits like Stamets purports with the LM supplements he sells advertised as grown on rice with the mycelium specifically being harvested


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OfflineStromriderM
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Re: Harvesting Mycellium (moved) [Re: SonOfAHooker]
    #28598671 - 12/26/23 02:40 PM (1 month, 1 day ago)

He just spews that nonsense so he can make supplements without having to fruit mushrooms


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OfflineManitobaSlim
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Re: Harvesting Mycellium (moved) [Re: Stromrider]
    #28601037 - 12/28/23 03:09 PM (30 days, 7 hours ago)



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Onlinecybork
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Re: Harvesting Mycellium [Re: Cassimere]
    #28605554 - 01/01/24 08:49 AM (26 days, 13 hours ago)

Here are some suggestions regarding harvesting mycelium. I thought it was an interesting question, so I googled and found some info. Frankly I think that some suppliers might just dry the substrate and the mycelium, and just grind all to powder and put those in capsules... If you are really interested in only mycelium, possibly you can grow mycelium on big agar plates, and scrape of the mycelium. Or something. Not sure how cost effective that will be.

Of course these suggestions beneath were made with the intent of information about how to separate soil from mycelium. And you are looking for the opposite. But it can help you in your search, I think. Some of the processes will get you what you ask for.

PS: I'm aassuming you really want to harvest the mycelium. And to be sure: mycelium='the vegetative part of a fungus, consisting of a network of fine filaments (hyphae)').

I found these on
https://www.researchgate.net/post/How_could_you_extract_mycelium_from_the_soil#:~:text=Some%20common%20methods%20include%3A,for%20large%20amounts%20of%20soil:

"
All Answers (2)
Nicolò M. Villa
Swedish University of Agricultural Sciences
Dear Megan Shoemaker ,
There are several methods that can be used to separate mycelium from soil, depending on the specific goals of your project and the resources available to you. Some common methods include:
Filtration: This method involves using a filter paper or mesh to physically separate the mycelium from the soil. This method can be useful for small-scale experiments, but may not be efficient for large amounts of soil.
Centrifugation: This method uses a centrifuge to separate the mycelium from the soil by spinning the mixture at a high speed. The mycelium will collect at the bottom of the centrifuge tube, while the soil will be suspended in the liquid medium.
Sieving: This method involves passing the soil-mycelium mixture through a sieve to separate the mycelium from the soil particles.
Decantation: This method involves allowing the soil-mycelium mixture to settle and then carefully pouring off the liquid. The mycelium will be collected on the bottom of the container, while the soil will be suspended in the liquid.
Flotation: This method involves adding a reagent to the soil-mycelium mixture that causes the mycelium to float to the surface, where it can be collected.
Sedimentation: This method involves adding a reagent that causes the mycelium to aggregate, and then the agglomerated mycelium can be separated from the soil by sedimentation.
It's worth noting that some of these methods may not be effective depending on the type of soil and mycelium you're working with, so it's a good idea to try out a few different methods and see which one works best for your specific project.
Cite
1 Recommendation
Ravini Vidushika Gunawardhana
Sabaragamuwa University of Sri Lanka
There are several methods for extracting mycelium from soil in mycoremediation projects, including,
Physical separation: This method involves physically removing the mycelium from the soil using tools such as tweezers or a scalpel. This method is generally used for small-scale extraction and is not suitable for large amounts of soil.
Washing: This method involves washing the soil mycelium mixture with water or a solution to remove the soil and leave the mycelium behind. The mycelium can be collected by using a filter or centrifugation.
Enzymatic digestion: This method involves using enzymes to break down the soil and release the mycelium. Cellulase or other enzymes that can degrade plant cell walls can be used to digest the soil.
Solvent extraction: This method involves using a solvent to dissolve the soil and leave the mycelium behind. Common solvents used include ethanol, methanol, and chloroform.
Incubation: This method involves incubating the soil-mycelium mixture at the optimal conditions for mycelium growth, and then removing the mycelium by washing or centrifugation.
Selective media: This method involves growing the mycelium on selective media, which promotes the growth of the mycelium while inhibiting the growth of other microorganisms in the soil.
Magnetic separation: this method is based on the magnetic properties of the mycelium. By using magnetic beads or nanoparticles that bind to the mycelium, it is possible to separate it from the soil through a magnetic field.
Microfiltration: this method uses a membrane with a pore size that allows the mycelium to pass through and retain the soil particles.
It's important to consider the characteristics of your sample, the pollutants you want to test and choose the appropriate method accordingly. Additionally, testing for pollutants can be done using techniques such as microscopy, PCR, and mass spectrometry.
Cite
"


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Offlinetaku
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Re: Harvesting Mycellium [Re: cybork]
    #28614482 - 01/08/24 08:18 PM (19 days, 1 hour ago)

i'm pretty sure those who use myc on rice just dehydrate rice and myc together and then blend them up.  when you look at LM pills done this way they usually state that there is rice in the ingredients. 

and yeah, the research is looking like the positive neurological effects of LM is hosted in the myc and less so in the fruit.


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