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OfflineNoncense
Saul Pamets
Male User Gallery


Registered: 12/23/21
Posts: 80
Loc: PNW
Last seen: 3 days, 5 hours
LAGM v. 2.024 * 9
    #28595514 - 12/23/23 04:49 PM (1 month, 4 days ago)

Hey Yall! Its that time of year again, Let all grow mushrooms 2024!

For the uninitiated "Let's All Grow Mushrooms" is a shroomery tradition where participants all grow mushrooms of any variety and species, updating their own grow logs along the way and watching other peoples grow logs take place. It's just one giant rootin tootin community grow-along! any and all are encouraged to participate!

If you're interested in taking place in this awesome tradition all you have to do is read the rules and reply to this thread! The spot where you reply to this thread will become your grow log that you update throughout the year. I will make you a link so it's easier for you and others to find your journals and post it on the first post of this thread. You can even put the link to your page in your signature so other people throughout the forum can see the progress you're making and know that you're super cool 🆒


HERE ARE THE RULES

- You must sign up. I guess you could just start posting in here without a page but that would be weird.
- Any and all species and varieties are welcome
- Whatever you're growing has to be started FROM SPORES, ON AGAR!
- We start January 1, 2024
- Don't be a dick! This is to have fun, show off a little and help others
- No Fun allowed this is a fun free zone.

how to sign up


- reply to this thread to reserve your spot. Your response will serve as your grow log for the year.
- In your response to this thread include what species and varieties you are going to grow
- If you're still confused just watch the first few happen and you'll catch on quickly.


*PRIZES*
Attention 2023 participants!
Last year we had quite a few folks volunteer prizes as well as myself and im not sure if people got them especially from me because I decided to have a mid life crisis and walk across the country for 6 months with a backpack on. If you want to cash in on a free swab feel free to shoot me a privnote and I will hook you up with something cool!

More Soon!

RockinRobot
Panaeolus

Pnin
Country Cock
Phobos
Lucid Gates

LandTrout
makarorae


EniQma
semperviva

KirkoftheIdaho
TBD

Gastronomicus
TBD

MojaveMike
Pan Cyan BVI
Australian Grasstree
MIB
Blue Springs Red Spore (BSRS)
Pan Jambo
P. Tampanensis Pollock
P. Cubensis Gandalf

BuzzPion
Golden Mammoth

shroom_sundance
AA+

Silentraindrops
B+
PF
PE

Nikoyo
Penis Envy
MVP

bigfootscreepyuncl
Pan. cyan
BVI, NecD, Bispo, Jambo, Wildcoast(?)

edixo
B+

AndImTheHighOne
Hanoi
Tosohatchee
Red boy revert
HBP
Natalensis


SeventhMushroom
Golden Teacher

Prim
TBD

ReverendMyc
Subtropicalis
Mexicana
Pan Cyan

Tiamo
natalensis
Pan. cyan

cyb3rtr0n
TBD

DirtBaggery
Thai Lipa

thirdeyewild
natalensis x tidalwave f2

EniQma
Jack Frost
Ghost
Wild Florida Cube
Natalensis
Pan Jambo
Thai Lipa Yai
Hillbilly
Pan BVI
GWM
Ps. Cyanescens
Wild ovoids


GogoBonkers
Liberty caps
PES Amazonian
B+
MVP

Vibetyme
TBD

mnj
Penis Envy
Albino A+
Pan Cam Thailand Suphanburi Goliath
Pan Cyan Hawaii

Noncense
TD
Steel Mag 5
Albino Thrape #1 (Nug) F1
A. Thrape #2 (FC) F1
A. Thrape #3 (LB) F1
Ps. Semperviva










Edited by Noncense (01/01/24 06:56 PM)


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OnlineRockinRobot
Stranger


Registered: 12/08/22
Posts: 861
Last seen: 9 minutes, 54 seconds
Re: LAGM v. 2.024 [Re: Noncense] * 3
    #28595519 - 12/23/23 04:51 PM (1 month, 4 days ago)

Count Me in


Got some Pan Prints coming so may as well.

Started 2 plates of Pans Cyan Texas Titan and Florida Blue Springs.




Well things are definitely moving now.

Spore plates for Texas Titans and Florida Blue Springs all germinated.




Transfer day. going to poke the first TTBVI plate for LC and also transfer to T2 for both TTBVI. Could probably spawn the first plate but would rather wait and spawn all 3 varieties at 1 time so will transfer again. The Texas Titans and Florida Blue Springs will get T1 plates made today as well. TTBVI not part of LAGM since I got impatient and put those spores to agar on 12/26/23 instead of 1/1/24 but thought I'd add them here anyway.




Have 8 jars of Rye Prepped, Knocked up a jar of LC from the TTBVI T1 when I did the Transfers to T2. 1 of the Florida Blue Springs and Texas Titan T1's look a little suspect.



Edited by RockinRobot (01/15/24 11:24 AM)


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OfflinePnin
Riz Gukgak


Registered: 07/18/23
Posts: 395
Last seen: 11 hours, 43 minutes
Re: LAGM v. 2.024 [Re: RockinRobot] * 1
    #28595572 - 12/23/23 05:38 PM (1 month, 4 days ago)

Alright, so far I think I'll be growing:

Country Cock
Phobos
Lucid Gates

I have a lot of things going at the moment but these I will dedicate for this thread.

Getting the SAB out today!

Streaked em. Made one plate of Phobos, and then one streak and swab plate each of the CC and the LG. My mail person hates swabs apparently because every single one was broken. :rockon:


1/5
Nats claimed. Might have more to offer in future. Will check on my plates and update tomorrow.

1/6
Seeing some contam and some action.



1/13
Finally clear enough in this flu that I was able to take some T1s. All of these look rough, the Phobos especially. I'm excited to see if I can clean them up alongside my other grows.




1/21
Transfer day. Two of these look like mold/contam to me but trucking on.





--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
>my end of 2023 grow journal<


Edited by Pnin (01/21/24 10:55 AM)


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OfflineLand TroutM
Stranger
I'm a teapot User Gallery


Registered: 01/08/18
Posts: 3,076
Last seen: 9 hours, 35 minutes
Re: LAGM v. 2.024 [Re: RockinRobot]
    #28595578 - 12/23/23 05:43 PM (1 month, 4 days ago)

I feel like it would be good to participate this year. As far as the rule of starting from spores on agar does that mean innoc day would be Jan 1 or after, or could I participate with my azurescens? X subaruginosa x allenii x cyanescens swinger party that I inoculated on 11/7? It’ll be an entertaining grow for sure.
Otherwise I’ll do a makarorae log here. Both will be slow AF.
Good to see you again nonsense! I hope your walkabout was epic! And happy shroomery birthday!


Edited by Land Trout (12/23/23 05:44 PM)


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OnlineEniQma
Registered: 11/28/23
Posts: 471
Last seen: 10 seconds
Re: LAGM v. 2.024 [Re: Land Trout]
    #28595582 - 12/23/23 05:44 PM (1 month, 4 days ago)

I’ll play.
But I have semp spores on agar inoculated last week that haven’t germinated yet
Does that count?


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OfflineKirkoftheIdaho
Practicer of Patience
Male

Registered: 11/19/23
Posts: 1
Last seen: 16 days, 13 hours
Re: LAGM v. 2.024 [Re: Noncense] * 1
    #28595607 - 12/23/23 06:02 PM (1 month, 4 days ago)

Yay something to look forward to!  I collected a print off of my first flush just this morning.


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OfflineGastronomicus
3-0-G
Male User Gallery


Registered: 03/31/05
Posts: 9,727
Last seen: 1 day, 15 hours
Re: LAGM v. 2.024 [Re: KirkoftheIdaho] * 4
    #28595619 - 12/23/23 06:09 PM (1 month, 4 days ago)

Yay I've been waiting for this!


1/3 Update

Finally got around to streaking plates. Running APE for the first time. Let's goooooo



1/12

Got some nice germination from a single point so took transfers tonight



1/24

Well I've been procrastinating working and posting but here's an update.

T1 transfers were looking really good, I was considering sending them but some small contams / sus parts started showing up so I took some T2's

Small rings on these two



Little nibble out of the side on this one



This pink boi




Still, the myc looks happy so hopefully I get something clean enough to put on grain with the T2's


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


Edited by Gastronomicus (01/24/24 10:38 AM)


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InvisibleMojaveMyc
Fractal Farmer
Male

Registered: 05/08/21
Posts: 655
Loc: Colorado
Re: LAGM v. 2.024 [Re: Gastronomicus] * 2
    #28595624 - 12/23/23 06:10 PM (1 month, 4 days ago)

Awww yeah! Claiming my spot. Actually going to keep up with it this year :lol: I’ll come back when I figure out what I’m gonna grow.
—————-
I’ll be running
Pan Cyan BVI, Australian Grasstree, MIB, & Blue Springs Red Spore (BSRS), TTBVI
Pan Jambo
P. Tampanensis Pollock
P. Cubensis Gandalf

Choosing these varieties because I only have one sample of each & am in need of more spores. I removed semperviva from the list because I realized I will run out of shelf space with the slowpokes I’m already running. Also why I chose fast stuff this year.
——————-
1/1:


Spores made it to agar today! I swabbed each variety to two plates I prepped last year (sorry, can’t help it lol). My agar recipe is 8g agar, .6g nutritional yeast, and 500ml boiled grain water. See you guys in a few days!

1/4:
We have germ on all of the pans :dancer: still waiting on Pollock & Gandalf. Here are some germ pics. Sorry for the low quality but I don’t have a memory card in my lil digital scope.

(Oops gotta change the date)



1/14:
Had a busy week or so, made my T1s on 1/9 but didn't have time to come make an update.

Germ plates:


Tamp Pollock germ plate is lookin moldy. We'll see.


Transferred.


Today, the T1s are looking good. I tried to select the thinnest, whispiest myc available from the Pan plates.

Gandalf:


MIB. I'll be taking the faster growth from plate #2.


BVI. Decent clean growth


Grasstree. Good growth on both of these plates, I'll be sending them straight to grain.


Blue Springs Red Spore. Feeling the one on the left in pic #1.


Jambo, undecided


TTBVI, nice & whispy


1/19: You can see last week’s temp swings in the myc. Kinda cool.

Slight scalloping on the Gandalf culture, gonna scrap it & go back to the germ plate


MIB: Might need another transfer. Scrapping the second plate


TTBVI: ready for grain


BVI: Taking 1-4 o’clock from pic 2 to grain


BSRS: taking the larger colony to grain.


Grasstree: Pic 1 is headed to grain


Jambo: going to grain




Edited by MojaveMyc (01/19/24 06:18 PM)


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OfflinePnin
Riz Gukgak


Registered: 07/18/23
Posts: 395
Last seen: 11 hours, 43 minutes
Re: LAGM v. 2.024 [Re: Land Trout]
    #28595644 - 12/23/23 06:33 PM (1 month, 4 days ago)

Quote:

Land Trout said:
I feel like it would be good to participate this year. As far as the rule of starting from spores on agar does that mean innoc day would be Jan 1 or after, or could I participate with my azurescens? X subaruginosa x allenii x cyanescens swinger party that I inoculated on 11/7? It’ll be an entertaining grow for sure.
Otherwise I’ll do a makarorae log here. Both will be slow AF.
Good to see you again nonsense! I hope your walkabout was epic! And happy shroomery birthday!




I am also hoping we can use spores on agar from before Jan 1 because I swiped almost all of my prints to agar right before I went on holiday...


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
>my end of 2023 grow journal<


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OfflineBuzzPion
Stranger


Registered: 12/06/23
Posts: 43
Last seen: 14 hours, 10 minutes
Re: LAGM v. 2.024 [Re: Noncense] * 1
    #28595695 - 12/23/23 07:19 PM (1 month, 4 days ago)

I’m in!

EDIT: Decided on P. cubensis "Golden Mammoth"

Update: 1-1-24 Inoculated 6 plates with a MSS. LME agar plates = 250ml H20, 5gr agar agar, 3.5gr LME. First time pouring plates, first time using the inoculation loop.





Edited by BuzzPion (01/01/24 07:47 AM)


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Offlineshroom_sundance
Some Guy
Male


Registered: 08/15/23
Posts: 16
Loc: rocky mountains
Last seen: 18 hours, 41 minutes
Re: LAGM v. 2.024 [Re: BuzzPion] * 1
    #28595735 - 12/23/23 07:50 PM (1 month, 4 days ago)

Reserving my spot.  going to use AA+ swabs that I just got.

1/2 Swabbed a couple plates, but of course forgot to take pictures.

1/16 Did my first plate transfer:




--------------------
LAGM v. 2.024


Edited by shroom_sundance (01/16/24 09:58 AM)


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OfflineSilentraindrops
mushlove student
I'm a teapot
Registered: 12/23/23
Posts: 222
Loc: pnw
Last seen: 7 hours, 38 minutes
Re: LAGM v. 2.024 [Re: Noncense] * 1
    #28595836 - 12/23/23 09:43 PM (1 month, 4 days ago)

To Op Change Pf , B+ , leucistic jedi mind fuck , ban hua thanon.
PF , B+ were started in December so may not be posted here at all. later
The LJMF and Ban Hua THanon are the ones i'm considering in the spirit of competition,

1/1/24 MSS to AGAR Jedi MF , Ban hua THanon.
Update: I failed at making good agar lol.... twice.... *this was 100% self inflicted* i let one this one cool to much had to microwave lol. both too stiff . this one has water on some still lol.
ANYWAYS.

1/7/2024. week update We have some colonization on all the JMF and maybe 1 on BHT






Update 1/12/2024 Transfer jmf + bht , will update pictures later.


Edited by Silentraindrops (01/13/24 12:22 AM)


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Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: LAGM v. 2.024 [Re: MojaveMyc]
    #28595841 - 12/23/23 09:54 PM (1 month, 4 days ago)

Quote:

MojaveMyc said:
Awww yeah! Claiming my spot. Actually going to keep up with it this year :lol: I’ll come back when I figure out what I’m gonna grow.

Does dried grain count? I have some stuff I’d like to include that I don’t have spores for haha





Spores to agar


--------------------



I :heart: 5318008


NOT a virgin!


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InvisibleMojaveMyc
Fractal Farmer
Male

Registered: 05/08/21
Posts: 655
Loc: Colorado
Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28595918 - 12/24/23 12:09 AM (1 month, 4 days ago)

Quote:

bigfootscreepyuncl said:
Quote:

MojaveMyc said:
Awww yeah! Claiming my spot. Actually going to keep up with it this year :lol: I’ll come back when I figure out what I’m gonna grow.

Does dried grain count? I have some stuff I’d like to include that I don’t have spores for haha





Spores to agar




Hey, it’s a fair question! Folks have been allowed sporeless varieties like enigma & ESS in previous LAGMs 🤷🏾‍♂️ Oh well, rules are rules :rockon:


--------------------


Edited by MojaveMyc (12/24/23 09:00 AM)


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OfflineNikoyo
FUN GUY
I'm a teapot


Registered: 01/29/17
Posts: 288
Last seen: 5 days, 14 hours
Re: LAGM v. 2.024 [Re: MojaveMyc] * 1
    #28596474 - 12/24/23 10:43 AM (1 month, 3 days ago)

Count me in! 2020 got pretty wonky and had to stop midway so ill finally finish this time around :drysmash: still currently waiting on some new stuff from the marketplace but heres what i plan on starting on the first while i wait for everything else to come in.

species: p cube
Varieties: pe, mvp. May add more to the list if they come in time.

1/1/24
4 plates of pe, mvp and atl7 (decided on one more to spice things up) using sterile swabs. See what we get.


1/9/24
Mvp and atl7 germ plates were looking pretty dang good so took some t1 transfers, pe germ plates arnt showing anything yet so back to watching paint dry on those:shrug: been pretty dang busy so skipping pictures on this update till theres more progress to show.

1/16/24
Mvp t1 plates are looking good already, will be taking t2 transfers tonight as well as some t2 transfers on the atl7 plates. Pe germ plates are finally showing some growth so will be taking t1 transfers. Hard to see in the pic of the pe plate with the condinsation but theres growth! Will proably do 2 more transfers on each till they are nice and uniform before sending to grain :dancer:


--------------------
Gettin back to work


Edited by Nikoyo (01/16/24 03:07 PM)


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Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: LAGM v. 2.024 [Re: MojaveMyc]
    #28596507 - 12/24/23 11:27 AM (1 month, 3 days ago)

Quote:

MojaveMyc said:
Quote:

bigfootscreepyuncl said:
Quote:

MojaveMyc said:
Awww yeah! Claiming my spot. Actually going to keep up with it this year :lol: I’ll come back when I figure out what I’m gonna grow.

Does dried grain count? I have some stuff I’d like to include that I don’t have spores for haha





Spores to agar




Hey, it’s a fair question! Folks have been allowed sporeless varieties like enigma & ESS in previous LAGMs 🤷🏾‍♂️ Oh well, rules are rules :rockon:





Yeah, fair. I'm the one who originally bent the rules and allowed sporeless varieties and clones and grains and whatnot to be used in LAGM and it turned into a shitshow..err, an even bigger shitshow :laughing:


granted, I'm not running the show this year so that could get overruled but my understanding is that this is a no-frills, authentic LAGM.

Also, ESS does in fact produce viable spores :thumbup:


--------------------



I :heart: 5318008


NOT a virgin!


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InvisibleMojaveMyc
Fractal Farmer
Male

Registered: 05/08/21
Posts: 655
Loc: Colorado
Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 2
    #28596551 - 12/24/23 12:21 PM (1 month, 3 days ago)

Heard! Yeah we don’t need any shitshows haha I rescind my request :lol: not trippin, just got some ESS (the ones making the rounds atm are sporeless so my bad) in the mail the other day & I’m just excited to run it. My spores are ready :dancer:

To atone & get back on track, I’d like to offer 5 mystery swabs/prints/wedges to first five growers to post a canopy :cheers: only one will be a cube.

Editing to sweeten the deal. Found more stuff. Let’s get back to having fun 😎


Edited by MojaveMyc (12/24/23 04:41 PM)


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OnlineRockinRobot
Stranger


Registered: 12/08/22
Posts: 861
Last seen: 9 minutes, 54 seconds
Re: LAGM v. 2.024 [Re: MojaveMyc]
    #28596587 - 12/24/23 01:08 PM (1 month, 3 days ago)

Quote:

MojaveMyc said:
Heard! Yeah we don’t need any shitshows haha I rescind my request :lol: not trippin just got some ESS (the ones making the rounds atm are sporeless so my bad) in the mail the other day & I’m just excited to run it

To atone, I’d like to offer 5 mystery swabs/prints to first five growers to post a canopy :cheers: only two will be a cube





Didn't I just send you semp print? Sounds like a good one for LAGM to me...


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Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: LAGM v. 2.024 [Re: RockinRobot] * 5
    #28599308 - 12/27/23 12:39 AM (1 month, 1 day ago)

Fuggit - I was hoping for this thing to make page 2 so I could hide my moldy tubs from the casual lurker :lol:


WITNESS THE SHITNESS
THAT IS BIGFOOTS GROWLOG




This shitshow is brought to you from sponsors such as CocoBliss, Sterlite and your mother. Made possible by viewers like you!


For the year of 2024 I will be up to my elbows in shit...literally! I am taking it upon myself to expand my horizons and focus ONLY on a species that I haven't ever run, Panaleous cyanescense (fuck off if you're spell checking me, you're not my Latin teacher!) Been meaning to do this for a long time just never have. I've got plenty of prints - we'll see if they're still viable. I've got a box of aged horse turds in the attic - we'll see if it isn't moldy or infested with bugs that I will not deal with. Poured several sleeves of plates a week ago - we will wait and see if they sprout satellites until it's inoculation time.

THE STAGE IS SET!


Will Bigfoots Creepy Uncle finally slay beast that has been daunting him for years, or will he wind up with moldy boxes of horse shit??
STAY TUNED!!!



Species: Pan. cyan
Varieties: BVI, NecD, Bispo, Jambo, Wildcoast(?)


12/27/2023:

12/28/2023: I just checked the box o' equine nuggets I've had in my attic for like 3 years and it looks mighty fine, so I'm good to go on substrate :rockon:

1/1/2024 (1/1/2024 for all you non-Americans): Pretty hungover but extremely busy. No cult work done today..

1/3/2024: Finally got around to streaking some plates. The whole SAB session felt pretty sloppy so we'll see what comes of it, but I've got spores on agar! BVI, NecD, Bispo, Wild Coast and Jambo plated :rockon: I didn't take any pics because they're just plates and the lids condensed pretty bad while I was working anyway so there's nothing to even see.

All of my prints (except Bispo) are pretty old so I'm not very optimistic. Jambo is 3 years old and spent a week in my mailbox at -40F, Wild Coast is 3 years old and the way it was packaged is pretty much just asking for contaminations, NecD is 2 years old and the 'print' was just a pile of spores and BVI, also 2 years old was an extremely faint print but mostly a pile of spores.


1/5/2024: Just as I expected, my freshest print, Bispo, is the first to show germination :rockon: LAGM is officially underway!



1/6/2024: Suspect BVI and Wild Coast have germinated. Nothing yet on NecD or Jambo but I'll remain patient and optimistic.

1/7/2024: Germination confirmed on BVI and Wild Coast :rockon:


Bispo is growing out nicely. Shouldn't be too long before I'm making my first transfers!


1/8/2024: Spotted satellite growth on my Wild Coast plate today. I believe it's actually a spore clump that fell off my loop as I was streaking this plate though. Either way I will continue to monitor it and if I have to I will take transfers earlier than planned since there is plenty of good growth to work with already.

Satellite growth on WC plate.

I'll know better in a day or two but I believe I spotted germination on my NecD :rockon:


1/10/2024: :fuckyeah: we've got germination on Jambo! Now all fighters have entered the ring, time to get rockin' and rollin' :rockon: Jambo is showing mycelial germination and also a very small bacterial colony right on my streak line - this one may be a challenge to clean but I'll give it my best shot. Everything else is lookin clean and proper. Thankfully I've still got about 15 plates ready to roll because I need to start makin' some transfers...goddamn I hate overgrown germ plates :lol:



1/13/2024: Finally got around to taking T1's on all my varieties :rockon: everything went smoothly except for one of my transfer pieces of Wild Coast - I could not get the transfer piece off of my scalpel so I fumbled with that a fair amount then when I was wrapping that plate the stubborn transfer piece went rattling around the plate :facepalm: oh well. If that's the worst that happens I can live with that. Depending on how these T1's grow out I will likely be sending T2's to grain and/or LC.



1/14/2024: Ooooo GodDAMN these suckers move fast! ~14 hours after making T1s:



Need to pour some more plates and cook some LC broth cuz at this rate I'll need to be getting T2 done in a couple days at most :lol:


1/18/2024: Waiting to hear back from some experts on this species but my plates are fucked. I thought these cultures were moving suspiciously fast, even for a fast species.... Bigfoots gonna be pouring some plates and going back to spore. What a kick in the dick lol. I'll post pictures later when I'm not completely deflated just to keep a thorough log.


Edited by bigfootscreepyuncl (01/18/24 12:49 PM)


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Offlineedixo
Fun Guy


Registered: 12/21/23
Posts: 91
Loc: Noosphere
Last seen: 2 hours, 11 minutes
Re: LAGM v. 2.024 [Re: Noncense] * 4
    #28599318 - 12/27/23 01:21 AM (1 month, 1 day ago)

Ah fuck, I am gonna have to do this.

Species: P. Cubensis
Variety: B+ from 2yo MSS
Method: Inoculation loop to 10 5 plates of agar
Spawn: Either brown rice or rye
Substrate: 1:4-1:5 spawn to pure coir and a fat pseudocasing
Tub tek: 15.5L EZ dialed in tubs with 3 IKEA® GLIS® boxes in each, filled with compressed substrate



01/01/2024

Happy New Year!

Got 5 plates inoculated with the 2yo MSS. This is the one I've previously had the best success with, so here's hoping I'll get some viable mycelium!



08/01/2024

Liftoff! We have germination on plate 1! Now we wait for more.



09/01/2024

Really nice growth on plate 1 already, so did a small transfer to some homemade agar to see if it work. If it does, I'll try to put it to grain.



12/01/2024

Plate 1 has some very even, solid growth going on, so did 3 transfers of that to some store-bought PCA. The homemade agar -seems- to be working, but it's slow.



15/01

Plate 1 is so far the only one with growth, but it's going so strong, and so are the transfers. I decided to put some to grain directly from the plate instead of waiting for the transfers to grow out.



26/01

Grain transfers are slowly coming in. However, something else has happened - I accidentally dropped one of the transfer plates while looking at it, which caused the transfer wedge to split into three. Two stayed in the middle, and another went to the edge of the plate. Some bacterial contamination sprung up right next to it, and lo and behold; the myc beat it. It's slowly surrounding the bacteria, and the myc growing from it is markedly thicker and more even than the other wedge pieces.

I think I'm gonna isolate it.



27/01

Not even 24 hours after transferring that contam resistant strain, and it looks like this. Exciting!



--------------------
~ LAGM 2024 ~


Edited by edixo (01/27/24 01:48 AM)


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Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: LAGM v. 2.024 [Re: edixo] * 1
    #28599333 - 12/27/23 01:54 AM (1 month, 1 day ago)

Quote:

edixo said:
Ah fuck, I am gonna have to do this.

Species: P. Cubensis
Variety: B+ from 2yo MSS
Spawn: Either brown rice or rye
Substrate: 1:4-1:5 spawn to pure coir and a fat pseudocasing
Tub tek: 15.5L EZ dialed in tubs with 3 IKEA® GLIS® boxes in each, filled with compressed substrate







I'm just making sure you understand what LAGM is all about here, incase you don't know..LAGM is all about spores to agar, not cakes (the brown rice made me think you were doing PF TEK)...

Originally LAGM was started to show cultivators new and seasoned alike that agar is nothing to be afraid of and that it's actually pretty damn easy!

Just wanted to make sure you're running agar not cakes!

If you were planning to run cakes because you've never played with agar hit me up and I'd be happy to give you some good links to agar TEKs or if you're desperate I'm sure we could work a way out for you to get some ready to use plates...

Happy LAGMing, I look forward to seeing what kind of weirdo B+ you end up getting :rockon:


--------------------



I :heart: 5318008


NOT a virgin!


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Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28599334 - 12/27/23 01:55 AM (1 month, 1 day ago)

Goddamnit here's page 2 that I was waiting for :rofl:


--------------------



I :heart: 5318008


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Offlineedixo
Fun Guy


Registered: 12/21/23
Posts: 91
Loc: Noosphere
Last seen: 2 hours, 11 minutes
Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28599335 - 12/27/23 02:07 AM (1 month, 1 day ago)

Quote:

bigfootscreepyuncl said:
Quote:

edixo said:
Ah fuck, I am gonna have to do this.

Species: P. Cubensis
Variety: B+ from 2yo MSS
Spawn: Either brown rice or rye
Substrate: 1:4-1:5 spawn to pure coir and a fat pseudocasing
Tub tek: 15.5L EZ dialed in tubs with 3 IKEA® GLIS® boxes in each, filled with compressed substrate







I'm just making sure you understand what LAGM is all about here, incase you don't know..LAGM is all about spores to agar, not cakes (the brown rice made me think you were doing PF TEK)...

Originally LAGM was started to show cultivators new and seasoned alike that agar is nothing to be afraid of and that it's actually pretty damn easy!

Just wanted to make sure you're running agar not cakes!

If you were planning to run cakes because you've never played with agar hit me up and I'd be happy to give you some good links to agar TEKs or if you're desperate I'm sure we could work a way out for you to get some ready to use plates...

Happy LAGMing, I look forward to seeing what kind of weirdo B+ you end up getting :rockon:



Wait, so it's ONLY to agar, not beyond that?

I was planning on doing like 10 plates with a noc loop I'm gonna build.


--------------------
~ LAGM 2024 ~


Edited by edixo (12/27/23 02:13 AM)


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Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: LAGM v. 2.024 [Re: edixo] * 1
    #28599337 - 12/27/23 02:23 AM (1 month, 1 day ago)

Quote:

edixo said:
Quote:

bigfootscreepyuncl said:
Quote:

edixo said:
Ah fuck, I am gonna have to do this.

Species: P. Cubensis
Variety: B+ from 2yo MSS
Spawn: Either brown rice or rye
Substrate: 1:4-1:5 spawn to pure coir and a fat pseudocasing
Tub tek: 15.5L EZ dialed in tubs with 3 IKEA® GLIS® boxes in each, filled with compressed substrate







I'm just making sure you understand what LAGM is all about here, incase you don't know..LAGM is all about spores to agar, not cakes (the brown rice made me think you were doing PF TEK)...

Originally LAGM was started to show cultivators new and seasoned alike that agar is nothing to be afraid of and that it's actually pretty damn easy!

Just wanted to make sure you're running agar not cakes!

If you were planning to run cakes because you've never played with agar hit me up and I'd be happy to give you some good links to agar TEKs or if you're desperate I'm sure we could work a way out for you to get some ready to use plates...

Happy LAGMing, I look forward to seeing what kind of weirdo B+ you end up getting :rockon:



Wait, so it's ONLY to agar, not beyond that?

I was planning on doing like 10 plates with a noc loop I'm gonna build.





No no no no it's absolutely beyond the plate. I'm hammered so I may not have explained it well at all...in fact I'm sure I didn't..

The whole thing is that you have to go spore to agar to grain to whatever..but your grow has to start spore to agar. You said MSS and brown rice so I was worried you were just shooting spores into cakes. My bad!

Fuck yeah, noc some plates and let 'er rip!! My inoculation loop is DIY to and it works like a champ!



Even kept a picture of it for this exact drunken moment. Much foresight. Such wow!


--------------------



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Offlineedixo
Fun Guy


Registered: 12/21/23
Posts: 91
Loc: Noosphere
Last seen: 2 hours, 11 minutes
Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28599339 - 12/27/23 02:28 AM (1 month, 1 day ago)

Quote:

bigfootscreepyuncl said:
Quote:

edixo said:
Quote:

bigfootscreepyuncl said:
Quote:

edixo said:
Ah fuck, I am gonna have to do this.

Species: P. Cubensis
Variety: B+ from 2yo MSS
Spawn: Either brown rice or rye
Substrate: 1:4-1:5 spawn to pure coir and a fat pseudocasing
Tub tek: 15.5L EZ dialed in tubs with 3 IKEA® GLIS® boxes in each, filled with compressed substrate







I'm just making sure you understand what LAGM is all about here, incase you don't know..LAGM is all about spores to agar, not cakes (the brown rice made me think you were doing PF TEK)...

Originally LAGM was started to show cultivators new and seasoned alike that agar is nothing to be afraid of and that it's actually pretty damn easy!

Just wanted to make sure you're running agar not cakes!

If you were planning to run cakes because you've never played with agar hit me up and I'd be happy to give you some good links to agar TEKs or if you're desperate I'm sure we could work a way out for you to get some ready to use plates...

Happy LAGMing, I look forward to seeing what kind of weirdo B+ you end up getting :rockon:



Wait, so it's ONLY to agar, not beyond that?

I was planning on doing like 10 plates with a noc loop I'm gonna build.





No no no no it's absolutely beyond the plate. I'm hammered so I may not have explained it well at all...in fact I'm sure I didn't..

The whole thing is that you have to go spore to agar to grain to whatever..but your grow has to start spore to agar. You said MSS and brown rice so I was worried you were just shooting spores into cakes. My bad!

Fuck yeah, noc some plates and let 'er rip!! My inoculation loop is DIY to and it works like a champ!



Even kept a picture of it for this exact drunken moment. Much foresight. Such wow!




Oh fuck no. I hope I will never have to shoot spores into grain again.

I've edited my post to make it clear I am gonna do spores to agar. :P


--------------------
~ LAGM 2024 ~


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OfflineAndImTheHighOne
Stranger

Registered: 12/06/23
Posts: 44
Last seen: 12 hours, 8 minutes
Re: LAGM v. 2.024 [Re: edixo] * 3
    #28599451 - 12/27/23 06:50 AM (1 month, 1 day ago)

Might as well sign myself up for this since I'm new and I'll be doing pretty much everything from spore to start 2024. All will be done with vendor-supplied MSS unless otherwise noted

I'll be growing (in no particular order):

Hanoi, Tosohatchee, Red boy revert (from swab), HBP (swab), Golden Koh F2, and Natalensis

I'm still waiting for my first PF jars to finish consolidating, but I've been using the last two weeks to brush up on my agar skills. So even though I'm feeling very good about this upcoming year, I might not be growing mushrooms from agar at all, stay tuned!

Edit: Going to be switching up a few of the varieties I'll initially be putting to agar. I got a little too excited and already used my only redboy swab (oops) and the HBP hasn't arrived yet, so those two are out for now. I'll still do Hanoi and Tosohatchee, and I would really love to grow Nats but all of this is super new to me. Fingers crossed.

The SAB will be all set up for when I get home tonight LOL. Depending on what condition I'm in I might get some of this going after midnight. I will probably pick at least one more strain of cube to culture, but I'll let the inspiration come to me in the moment.


How's my setup? Lol


**UPDATE 1/1** Happy New Year!! Let's Grow Some Mushrooms!

OK, so I put drops to all of my germ plates this afternoon. 11 plates total, 4 cube varieties cultured, and P. natalensis. All from MSS. Went with the aforementioned Hanoi and Tosohatchee, also threw in some Golden Koh F2 and Ecuador. Now we wait!!



**UPDATE 1/4** Could... Could It Be?

Well, something is happening with one of my plates. When I first glanced at this my heart sort of sank because I was pretty damn sure it was bacterially contaminated. Oh well. We were bound to lose a few.

But when I went back to take a picture for this post, I realized I might have been too quick to judge. I remembered that while I did try my best to shake up the Golden Koh syringe I was using, I still wound up shooting a large, visible pool of spores in the single drop I placed on the plate. I did my best to spread them out without my hand hovering over my work and just sort of crossed my fingers that everything I did was sterile because I'd hate to lose all those spores!



So still TBD what I'm working with here, but I'm a lot more hopeful about it than when I first checked.

That was the only plate showing any activity so far. Staying patient!


**UPDATE 1/6** MYC CHECK, MYC CHECK!

Very excited to make an update this morning because we have action all over, ladies and gents. The spots I saw on the Golden Koh plate have now turned into little balls of cube mycelium.



A little hard to tell from the lighting in these pics (I tried a bunch of different setups and these were the best ones,) but those spots are nice little white, fluffy guys.

Given my lack of experience, I had a hard time at first distinguishing spores germinating on agar from bacteria. Now that I have a better idea of what I'm looking at, I can pretty confidently say that the Hanoi, Tosohatchee, and Nat plates are all showing signs of germination.

I have no clue what is going on with my Ecuador syringe. I inoculated three plates with it on 1/1 because I'd already put a couple drops to agar weeks ago and nothing happened. Literally nothing at all. Well, nothing is happening again. On all three plates. Bad syringe? I will probably use it on a few PF jars and see if anything happens :shrug:


**UPDATE 1/9/24** First Transfer and Other Things


FIRST TRANSFER!!!

A slightly overdue update today. I could have easily made my first transfer on the 7th, but I had a busy weekend and was not well-prepared to do so. The Golden Koh plates are still my superstars of the bunch, but the Nats are also taking off.



I can and probably should take a transfer from both Nat plates today. As you can see, I did take the first transfers from the best looking sections of my more advanced G. Koh plate. I'm probably going to wait until the bottom one grows out a bit more before I pick which section(s) to take. I'm open to suggestions!



The Tosohatchee and Hanoi plates are also growing nicely(ish). My Tosohatchee genetics seem to express as a lot of very thin, whispy mycelium, but it looks healthy for the most part, and much better on my T2 and T3 plates that were started last year. I can probably make transfers from any of these plates today.

Unfortunately nothing to report on the three Ecuador plates (I won't take pictures of those,) and I'm soon going to give up hope on them. I saw what I thought might be germination while examining them closely last night, but I think it was just a very faint mark from where my inoculation loop touched the agar while streaking the plate.


**UPDATE 1/16/24** (!) All T1s Complete (!), First Plates Contam, Bad Ecuador, and My Plan Moving Forward

Alright, alright, alright we've got a lot to cover as this is my first update in A WEEK! For anyone who is following along, I certainly don't want to make you wait too long between updates and lose your interest. But what is LAGM without a bit of suspense, right?!

So without further ado, I present my eight T1 transfers:



Organized by row, from top to bottom, we have: Tosohatchee, Hanoi, P. natalensis, and Golden Koh F2

Despite having quite a busy week, I didn't completely neglect my cult work. I took the remaining six transfers from my six different germ plates on 1/13 (Yes, the plates say the 12th. I was tired and mislabeled them. I haven't bothered to fix it. Sue me.Please don't) Feeling pretty good about these T1s. No satellite contams have appeared... yet. I'm seeing enough good growth that I could easily transfer away from anything that might pop up. I especially am liking the way my Nats plate on the left is looking already. Not even really sure what to make of the weird patterns on the golden Koh plates. I believe I did those back when I still did transfers like a moron and would put the myc side down on the new plate, suffocating the myc in agar. I don't do that anymore lol. I am planning to take my T2s from those plates today or tomorrow, and it looks like I'll have to be careful to take only small chunks to try to ensure I'm grabbing clean culture.

Not all the news is good. If you saw my earlier update and thought the specks on my other Golden Koh germ plate looked kinda sus, you were correct to have your doubts. It was a little difficult to tell at first, but it looks like this now:



Fortunately, I already have the two T1s from the other germ plate I took back on the 8th, so I probably won't even bother with this one. It is kinda awesome looking though, isn't it?

Well, there's nothing awesome about my Ecuador plates. I decided to re-inoculate them on the 11th because, why the hell not? The plates were just sitting there, still fresh and clean, and I didn't want to throw them out. I also figured it would be nice to check the syringe in question a few more times on plates I didn't care about. Well, something is growing on one of those plates now, but it sure as hell ain't Ecuador var. cubensis mycelium. :facepalm::



Got me at first too because when it first started out, it looked like it could've been germination. I suppose it was, just not the helpful kind that I am still so obviously learning to identify.

With T2 transfers on deck, I figure we can see how those look before making any concrete plans on how to proceed with actually growing these bad boys. Part of me is still loving the entire process of the PF Tek. I love the simplicity of the tek; I'm not in this for massive yields. It also doesn't require me to make my house smell like cooked fucking birdseed because that's the most readily available grain for my spawn at the moment. So we'll see I guess, but if my T2s look anywhere near the way they are starting, I'll probably consider doing some combination of sending a bit of that agar to grain, while also taking some clean T3s (*inshallah*) and preparing some of those plates to make LI for inoculating PF jars. I plan to work my way up to the monos at some point fam, but so far I only have experience with PF Tek, shoeboxes, and I'm currently attempting to fruit some bacterial spawn straight out of the jar. Baby steps, y'all! Thanks for reading!


**UPDATE 1/22/24** No Pics, Just Pain

Well, that was terrible... By far my sloppiest SAB session since the first time I tried using agar, maybe even more so. Really pretty unhappy with myself. I should have stopped when I first felt myself rushing and getting worked up by mistakes that normally wouldn't bother me so much. I decided to power through, and ended up making even more, bigger mistakes. Shocking!

So I'm going to wait a couple of days for my next picture update. I'll be holding my breath that the rest of my T2s aren't completely fucked.

Edit: Okay, I figured it could be of some value to type out exactly what went wrong this evening. It's helpful for me to write these things out, so that I won't make any of the same mistakes again. Also, for any other beginners following along that may have the same or similar processes as I do, this may serve as a warning or a reminder of potential pitfalls to avoid.

Alright, so first I'll explain my new process for doing transfers, before I get into how I messed it up. I just recently started using a long stainless steel straw to cut perfect circular pieces from my transfer plates. This was my second time using it, and I really enjoyed it the first time for a couple of reasons:

1) I absolutely love the perfect circles; they look very nice and it's easy to tell early on if the myc is growing out uniformly on your new plate

2) There's no need to hold the transfer plate to keep it from sliding around while you cut. Just push down with the straw, scoop up with a scalpel, and move it to the new plate.

The downsides:

1) You have to re-sterilize two different tools, and the straw takes a while to get red hot. Uses a lot of butane.

2) Depending on the size of the circles you cut, it may be difficult to get the circle off the scalpel and onto the new dish.

3) The end of the straw is much blunter than a scalpel blade. So if your myc is super thick/fluffy, it's hard to cut through with the straw and some will just push down the sides where the straw enters. Not the end of the world, but frustrating and confusing at first to say the least.

Downsides #2 and #3 were my biggest sticking point this time, and unless I get better at that part, I almost prefer cutting squares and triangles.

When I make transfers, here's everything I have in my SAB:
–#3 scalpel with a #10 blade (I hear #11s are the way to go and I will have to get some of those)
–Stainless steel drinking straw
–A ~1.5" section of clingwrap
–Surgical tray with all the previous things on it
–A stack of transfer plates
–A stack of fresh agar plates placed back in their original sleeve
–Two metal racks (a bigger one that holds my tray and transfer plates, and which I also do my work on; and a smaller one that I keep the stack of fresh plates on)

The SAB is wiped down somewhat half-assedly. Then, before going into the SAB, everything else is wiped with Lysol/Clorox disinfecting wipes or 70% iso soaked paper towels — whichever is closest to me when I start that part. Everything I'll be using inside the SAB is then covered each with a single paper towel to avoid it getting wet, then I give the entire box a good spray of tap water in attempt to clean the air of contaminants and wait ~5 minutes for the droplets to settle before starting my work.

I take a plate from my transfer pile, hold it up inside my SAB to the light to confirm which sections I had previously identified as my transfer pieces, then remove a single new dish from the sleeve, take the cling wrap off the transfer dish and place it gently back on the rack. I am right-handed, so my transfer dish is to the right of my new plate so that I never have to be over the new plate while it is open. Then, I take my straw outside the SAB and flame sterilize it until glowing orange (up to 30s) and carefully re-enter my SAB with it. I then turn my tranfer plate to a spot with no myc, slightly open the plate with one hand while still holding the lid and with the other hand push the end of the straw into the agar to cool it. With the dish still open, I turn it to where I intend to take a transfer and cut a circle, then close the dish. I then take my scalpel from the SAB and flame sterilize it, carefully re-enter my SAB, turn the transfer plate back to the open spot, open the plate just enough cool my scalpel in the middle of the spot where I did the straw and then scoop the little circle onto my blade, close the transfer dish and open my fresh agar plate, and slide the piece onto the surface. This part can be pretty tricky, as the circle is no bigger than my blade and hardly any of it hangs over the side to catch onto the fresh agar. So a couple of times I've had to scrape the transfer piece myc side down and then try to flip it over. It gets the myc all wet and gooey and I hate doing it, so I try to slide it off right side up, if at all possible. I also hate carving up my beautiful, fresh agar plate, so it's sometimes a lose-lose situation.

After one decent (but pretty ugly) transfer and then 3 absolute dogshit transfers in a row that wouldn't come off the scalpel, I was fit to be tied. Again, I should've stopped right there because usually I can roll with those kinda punches, but just couldn't tonight. It's also hot as fuck in my house right now with my dehydrator running and trying to keep my A/C off while I do sterile work, so I was sweating my goddamn balls off. I kept having to stop and take my hands out of the SAB to wipe sweat out of my eyes and then re-sanitize my gloves before resuming work. It was annoying. So now I'm sweating and rushing to get through the six remaining transfers I had to do, with a new process that I don't even really have the hang of yet, and it was messy yall. Filleted a few new plates and chopped up half my transfer pieces. My Nats were so fluffy and beautiful and it looks like the straw just stripped all the myc off the surface of the circle and down the sides, so that's cool.

Lesson learned is don't rush!! And if you feel like you should stop while you're ahead, do that! Also don't try to use a blunt object to cut super thick mycelium. Just use a scalpel (duh!)

Hopefully the Myco Gods were smiling upon me, and I can get a few decent transfer from all this mess. Goodnight everyone, and thank you for coming to my TED Talk.






Edited by AndImTheHighOne (01/22/24 11:18 PM)


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OfflineSeventhMushroom
just a tiny agar pin


Registered: 12/30/22
Posts: 55
Last seen: 1 day, 22 hours
Re: LAGM v. 2.024 [Re: Noncense] * 5
    #28599638 - 12/27/23 09:39 AM (1 month, 22 hours ago)

Reserving my spot for this year.

Just going to do only one spore sprint of golden teachers. Technically will be the first time I actually use a spore print instead of something else. Wish me luck

Species: P. Cubensis
Variety: Golden Teachers

Day 0

Kicking off the new year! Started off getting everything ready, then realized.. I have no "swab" like utensil. Nor do I have any sterile water to mix the spores with. So, I'm just going to be using scraping the spores directly from the print, with my inoculation loop. This seems less than ideal, since it's solid metal, and likely wont grab many spores, but I mean, technically just one spore needs to come along for the ride, right?

It's also a chance to test out my new flow hood I made!! More on that later, in another thread, but I built it to meet all the requirements for a flow hood from my 'research' on the topic. I used it to pour agar plates for the first time a week or so ago, and seems to have worked splendidly! I don't see any contamination on any of the plates. time will tell if this is repeatable :laugh: (my success rate with a SAB has not been great..)

(plates are LMEA with 2% agar, and 0.1% LME iirc? maybe should have documented that better somewhere.. hm.)

Here's one of the spore prints I'll be starting with :3


I started using the loop to scrape some spores off the print, then wipe it over the plates, but I wasn't sure I was really getting any spores. I guess time will tell. after a few plates, I tried *sanding* the surface of the loop to get more surface area for the spores. After that, I could visibly see spore clumps sticking to the loop, so I have better confidence on that.

Overall process was simple. Flame the loop. Run the print. Wipe on the plate. Repeat. Total of 5 plates, using two golden teacher spore prints. (I also used some LC to inoculate some jars I had, unrelated to LAGM in the same sitting)

And, my work is complete!



Day 11

First sign of life! 3 of the 6 plates have something fungus-like growing! I can't get a nice picture right now, sorry.

And, it seems the 3 plates that have germinated are the 3 plates that I made after sanding my inoculation loop to get a more "grippy" surface texture to grab spores (since, well, no swabs were available). So, I guess maybe that worked?

(picture taken on Day 20)


Day 17

I made a risky move today, and took some transfers directly to some LC vials. Either it works, or it won't. While I wouldn't advise others try it, I think it's going to be fine.. The plates themselves look rather clean, each one has a single germination spot of white fluffy mycellium. I'm pretty sure there's no mold at least. And, if there's bacteria, it will show up quickly in the vials in my experience. Call me lazy (: I didn't have any plates ready, but I some extra sterilized LC vials around...sooo...

The plates have also been very slow to grow, I'm assuming because it's been pretty cold where they are.

As for the transfer procedure, it was pretty straightforward: I use a pair of tweezers to remove the stopper from a vial under laminar flow, and place the stopper down upside-down. Take a tiny spec of myc from the plate with a flamed scalpel, and carefully wipe the myc off the blade into *the stopper*, the little well in the middle. It seemed easier than trying to shake it off directly into the vial, or scrape it off the side of the glass vial. The inside of the stopper should be as sterile as can be. Then, some tweezers to put the stopper back on.

(need to find out how to rotate this...)



Day 20

My LC vials seems to be okay! I'm getting the nice, fuzzy growth I'm used to seeing, and I don't see any signs of cloudiness that I get from bacteria. Usually by day 3, the liquid gets noticeably cloudy from contamination in my (limited) experience. I also moved the vials from the cold closet, to a warmer space inside, and over the last 24 hours, there has been significantly more growth. Go figure.

Here's a close-up of one of the vials, after swirling the contents around. You can see the little bit of agar, and the fuzzy "halo" around it as it's grown.





Edited by SeventhMushroom (01/20/24 03:22 PM)


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Offlineprim
school cafeteria bully


Registered: 09/18/23
Posts: 47
Last seen: 5 days, 19 hours
Re: LAGM v. 2.024 [Re: SeventhMushroom] * 1
    #28599671 - 12/27/23 10:23 AM (1 month, 21 hours ago)

this is such a neat idea and i look forward to participating.
i try to grow something new every time and so to be determined.
reserving my spot!


Edited by prim (12/27/23 10:25 AM)


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: LAGM v. 2.024 [Re: prim] * 6
    #28599744 - 12/27/23 12:10 PM (1 month, 19 hours ago)

Count me in please.
Let's go with:
  • Subtropicalis (RockinRobot's Bell Caps) print
  • Mexicana Chicon Nindo print
  • Pan Cyan Bunnell print
    Added
  • Subtropicalis (0t0lerance) swab
  • Subtropicalis (Hindsight) swab
  • Subtropicalis Xica swab


I have several other things starting up now as well, but these are the ones I will do from spores on 2024-01-01 (the correct date format:nerd:).


2023-12-30
First update - Pre-Prep work. Making up some fresh agar plates. 16g LME, 18g Living Jin Agar Agar, 1000ml distilled water in pp pitcher in Instant Pot for 50 minutes after 10 minute vent. Pouring into 100mm Celtreat grip ring plates in front of my ffu.

2024-01-01
Put spores to grain after finishing the final season of Letterkenny and a Doctor Who episode. The original three prints and added three swabs of different lines of subtropicalis that arrived in time to play. I also have several other pan varieties on plates that I started before the first, so they are not going to be featured here.


I track everything with a serial number in sharpie and a spreadsheet for all notes.


2024-01-04
Got some action on Pan Bunnell and the Subtrop Bell Cap!
Not all good. Bacterial brain blob.


But some good myc on both pan bunnell plates.


And stoked to see the subtrop bell cap already germing on one plate.


There might be some action on some of the rest, but too early to be certain.


2024-01-08
Transferred the most aggressive two plates to some new plates - Subtrop Bell Cap & Pan Bunnell
Mexicana Chicon Nindo from print & Pan Cyan Bunnell from print both showing some signs of life
Still waiting on the swabs to get started.


Also did some other things that I started before the 1st.


2024-01-10
Definitely seeing germ on Mex Chicon Nindo and Subtrop Hindsight. Hints of germ on the final two as well.

2024-01-12
Out of town for a while and missing them already. They grow up so fast.

2024-01-18
Home for a few days so checking in.


Here is the whole gang including both germs plates and the last 4 are t1s. Looks about time to get t1s from one of the Mexican Chicon Nindo and both of the Subtropicalis Hindsight. Still no action on the subtrop 0t0lerance and xica but I am patient.


The subtropicalis bell cap t1 plates already look really nice and may get a t2 and sent to grain soon. The pan bunnell are not as pretty but may get the same treatment because in my VERY limited experience with pans, sometimes the ugly plates out perform the pretty ones.

2024-01-19

t2 from the pan bunnells and t1 from one Mex Chicon Nindo and two Subtropicalis Hindsight. Going to do t2 transfers from the subtropicalis when I put them these plates to grain in a couple of days if the look worthy. t1 is usually too early for that, but I don't mind wasting a couple of jars on a chance.

Also started some Golden Teachers from prints to plates and prints to swabs to pucks for another couple of projects.
Its a grow off!
Plates vs pucks.
Used a print to do plates using a loop and two swabs dipped in sterile water and wiped on print to pucks. Lets see what happens.


2024-01-24
Home again so update time. Started prepping some grain for the subtropicalis bell cap t1s and 15 other non-lagm plates that are about ready.
Grabbed update pix of transfer plates.


pan bunnell t1 and t2.


mexicana chicon nindo and subtropicalis t1s


golden teacher nothing to see yet

Plan to get a t2 and put these t1 subtrop bell caps to grain tomorrow when the jars finish cooling.


2024-01-23
I actually executed the plan. Don't tell my wife that I can do that.


subtropicalis bell cap went to t2s and 2x1 pint millet grain jars.


Edited by ReverendMyc (01/25/24 09:28 PM)


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OfflineTiamo
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Re: LAGM v. 2.024 [Re: ReverendMyc] * 2
    #28599833 - 12/27/23 01:36 PM (1 month, 18 hours ago)

Reverend, I always knew you were a man of culture. ISO 8601 for the win.

I want to do a Ps. natalensis en Pan. cyan grow for LAGM 2024. Starting from my own prints, which I have yet to grab since I am working witb other people's prints now.

Taking it from spores to clone and testing the potency of the dried fruits along the way.

Let's have some fun. :rockon:


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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Re: LAGM v. 2.024 [Re: Noncense] * 1
    #28600060 - 12/27/23 05:06 PM (1 month, 14 hours ago)

I'll be starting some germ plates for this on my days off, either Sun 1/7 or Tues 1/9.  I don't want to rush things on a work or class day.




Edited by cyb3rtr0n (01/03/24 07:01 PM)


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Re: LAGM v. 2.024 [Re: cyb3rtr0n]
    #28600070 - 12/27/23 05:15 PM (1 month, 14 hours ago)

might as well give it try! Right now the only thing I have on swab/print is Thai Lipa but lets run it!


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Re: LAGM v. 2.024 [Re: Tiamo]
    #28600074 - 12/27/23 05:21 PM (1 month, 14 hours ago)

Quote:

Tiamo said:ISO 8601 for the win.



:highfive:
Team 8601!

Quote:

2024-01-21
Ima slide in all :ninja: like. Here be some nats:

nats froma da spors, producing biblically accurate angel

I R new to the species, so let's all gather round and watch 'ol stubb make a fool of himself. :borfase:




Quote:

2024-01-26

:jesusmagic:




--------------------
:mushroomgrow:
🆃🄴🅰🄼  🅲🄻🅸🄽🅶🅆🆁🄰🅿

You wake up. The room is spinning very gently round your head. Or at least it would be if you could see it which you can't.
It is pitch black.

> TURN ON LIGHT


Edited by stubb (01/26/24 11:18 AM)


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OfflineSeventhMushroom
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Re: LAGM v. 2.024 [Re: stubb] * 2
    #28600128 - 12/27/23 05:53 PM (1 month, 13 hours ago)

🆃🄴🅰🄼  🅸🅂🅾🄋


--------------------
LAGM 2024


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Re: LAGM v. 2.024 [Re: SeventhMushroom] * 1
    #28600169 - 12/27/23 06:38 PM (1 month, 13 hours ago)

I think I'll join this time

I have a nat x tw hybrid that I back crossed with jack frost. I'm going to grow out the f2's if I can find the swabs.

Jan 2nd update. Found the swabs, there were two , looked pretty light. I'll give them a week, if no growth I'll dig up another interesting cross. Maybe brainfreeze...

Jan 14: germination! Going to cook up some agar tonight for the transfer.


--------------------


Edited by thirdeyewild (01/14/24 07:40 PM)


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Re: LAGM v. 2.024 [Re: thirdeyewild]
    #28600199 - 12/27/23 07:10 PM (1 month, 12 hours ago)

What’s the objective? First canopy?


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Re: LAGM v. 2.024 [Re: EniQma]
    #28600225 - 12/27/23 07:32 PM (1 month, 12 hours ago)

Still not in a position to grow right now being in a shared house and all that jazz but love following the lagm threads so I'm excited to see what all you fine ladies and gentlemen and whoever else can get done this year!


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: EniQma]
    #28600243 - 12/27/23 07:51 PM (1 month, 11 hours ago)

Quote:

EniQma said:
What’s the objective? First canopy?





Not really any specific objectives necessarily..just a bunch of people growing fungus together, documenting their progress along the way!

In the past there have been prizes for first fruit, first canopy, biggest blah blah blah but that is 100% up to anyone who wants to put up a prize.

LAGM got started some time ago in order to show beginners that agar isn't scary or very difficult. The idea was that there would be a community of people who understood agar that would help beginners out as they needed it to move on from shooting spores into grain jars. Very noble origins indeed! Now it's mostly a dick measuring contest amongst assholes :rofl: (who are still very willing to help beginners if they need it :smile: )

Go back to page 1 and you can see my example of how I started my grow log. Your grow log will be the post I'm responding to but you continuously update/edit it throughout the year, or however long it takes you to accomplish your goal.

So hurry up and join it's super fun!!


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28600246 - 12/27/23 07:56 PM (1 month, 11 hours ago)

How do I join and is agar a requirement? For finding clone material my go to procedure is dropping spores from a print to a small amount of oats in a quart jar then adding a top layer of coir once colonized. I do use agar but not for speed and leisure


Edited by EniQma (12/27/23 07:56 PM)


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InvisibleWyoMX
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Re: LAGM v. 2.024 [Re: EniQma]
    #28600251 - 12/27/23 08:01 PM (1 month, 11 hours ago)

Quote:

Noncense said:

.....
HERE ARE THE RULES

- You must sign up. I guess you could just start posting in here without a page but that would be weird.
- Any and all species and varieties are welcome
- Whatever you're growing has to be started FROM SPORES, ON AGAR!
- We start January 1, 2024
- Don't be a dick! This is to have fun, show off a little and help others
- No Fun allowed this is a fun free zone.

how to sign up


- reply to this thread to reserve your spot. Your response will serve as your grow log for the year.
- In your response to this thread include what species and varieties you are going to grow
- If you're still confused just watch the first few happen and you'll catch on quickly.....





Yeee spores to agar is how everyone should start.


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Re: LAGM v. 2.024 [Re: WyoMX] * 2
    #28600275 - 12/27/23 08:22 PM (1 month, 11 hours ago)

Ok, so I’m going to make this the post I add to right?

I will be starting germ plates on 1/1
I’ll be doing no pours in small recycled glass jars with polyfill filters.

I have these to choose from, maybe someone could help me pick 3 since I already have a number of projects underway.

Jack Frost
Ghost
Wild Florida Cube
Natalensis
Pan Jambo
Thai Lipa Yai
Hillbilly
Pan BVI
GWM
Ps. Cyanescens
Wild ovoids I found

Seriously though, my interest is yours so lmk which 3 I should do. I wanna see some diversity around here!

I hope this isn’t going to get me disqualified!


So it’s 12/28/23 and I got my agar cooked.

I usually just wing it but this time I made a mental note to share with you fine folks. Could be soft, could be hard, time will tell…
I brought 250g water (ghetto I know) to a boil and slowly added 2.5g agar agar powder and 2.5g brown rice flour while whisking with a fork.


That was enough to make 9 of these plates(actually jars) and make them thicc. Just the way I like it 😉
Too bad I could only fit 8 in my presto

Prompting me to do a second batch.

I let the cooker vent for 10 min without the weight. Timer begins when the stopcock?(I think it’s called) was fully erect.

And once reaching 15psi I cooked for 20min

And two pro tips for any noobies,
1. Take them out hot to solidify on a flat surface.
2. Use a piece of paper towel between polyfill and foil to prevent issues with bacteria(that could occur prior to inoculation)


It’s 1/1/24 Happy New Year!
To kick it off I chose…
1. Natalensis
2. Ghost
3. Wild Ovoid

First I let my filter run 10 min. I keep it covered so nothing settles on the surface anyway, but it’s just a precautionary measure.

Then I wipe the desk and grates down with 70% iso


I proceed to do the same with my jars making sure to get up under the tub of the lid with the paper towel


Then the prints come out, and instead of using loop I just use my sterilized qtips.


It’s hard to take pictures while doing the work because I like to be fast and thorough, but I keep them as close to the flow as possible without touching and never cross anything in the path of flow from the fan to the media.


1/9/24
First germination noticed
Wild ovoid

And ghost

Still nothing on the nats

1/11/24
T1 ovoids and ghost



The ghost looked kinda baccy to me so I did a josex poke. It’s funny cause I was anticipating the ovoid to be bacterial since I found it wild.


Edited by EniQma (01/12/24 08:05 PM)


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OfflineCocaineBuffet
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Re: LAGM v. 2.024 [Re: EniQma]
    #28600309 - 12/27/23 09:20 PM (1 month, 10 hours ago)

Quote:

EniQma said:
Pan Jambo
Pan BVI
Wild ovoids I found




:smirk:


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Re: LAGM v. 2.024 [Re: EniQma]
    #28601507 - 12/28/23 08:16 PM (30 days, 11 hours ago)

Quote:

EniQma said:

Ghost
Ghost
Ghost






--------------------



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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28601575 - 12/28/23 09:14 PM (30 days, 10 hours ago)

Boom! First update! What was the prize for that?! :lol:


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28601576 - 12/28/23 09:16 PM (30 days, 10 hours ago)

Might as well give it a shot!

First focus will be working with some South African Transkei spores.

TOC:
Update #1 - SAT spores to agar


UPDATE #1 - SAT spores to agar


These aren't my best agar plates lol. If shit goes south then I have plenty of the original print to reset with. Hopefully I will have something in a couple weeks to transfer over to another set of agar plates.


--------------------
LAGM 2024


Edited by YellowBelly (01/03/24 08:24 PM)


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OnlineEniQma
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28601619 - 12/28/23 10:08 PM (30 days, 9 hours ago)

Quote:

bigfootscreepyuncl said:
Boom! First update! What was the prize for that?! :lol:



Damnit you beat me to it. Is there a silver medal?


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: EniQma]
    #28601707 - 12/29/23 12:42 AM (30 days, 6 hours ago)

Quote:

EniQma said:
Quote:

bigfootscreepyuncl said:
Boom! First update! What was the prize for that?! :lol:



Damnit you beat me to it. Is there a silver medal?





Bro you said erect cock in a non-phallic way. You just won LAGM


edit: also my update was verifying that my box of shit wasn't a moldy box of shit lol. I'll concede this victory


Edited by bigfootscreepyuncl (12/29/23 12:43 AM)


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OnlineEniQma
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28601791 - 12/29/23 04:55 AM (30 days, 2 hours ago)

Quote:

bigfootscreepyuncl said:
Quote:

EniQma said:
Quote:

bigfootscreepyuncl said:
Boom! First update! What was the prize for that?! :lol:



Damnit you beat me to it. Is there a silver medal?





Bro you said erect cock in a non-phallic way. You just won LAGM


edit: also my update was verifying that my box of shit wasn't a moldy box of shit lol. I'll concede this victory



:lolsy: Been growing phalli since 2012!


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OnlineSupaThaRipper
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Re: LAGM v. 2.024 [Re: Noncense] * 1
    #28601846 - 12/29/23 06:32 AM (30 days, 1 hour ago)

I’m doing a million projects right now but I’ll highlight this one for LAGM!

1-3-24
Psilocybe Zapotecorum var. Jalisco - Domestic F1



Starting with 2 swabs. I’ll smash one swab in water agar dish. Then with the other swab, I’ll streak a nutrient dish, and then smash another plate with that same swab.

Agar Recipe I’m using for nutrient:

500ml h20
10g agar
2.5g dextrose
5g sorghum syrup
.4g yeast
.10-15g peptone






1-7-24
I have growth, going to make a transfer and keep this germ plate on the backup as well.



1-15-24
Nutrient host plate and donor plates came up with trich. (Or some other bullshit contaminate. I don’t really care what kind it is. I just can’t have it.



Water plate is looking ok. Took some transfers and transplanted the original swab to brf pucks to give me better odds of success.




1-20-21

Having a lot of fun with these trich loaded swabs. Both transfers came up heavy with trich. Went back to the second donor plate and found it with trich as well. Managed to find what appears to be the cleanest spot and took 2 more transfers. 🤞

It appeared to be a clean dish when looking at it by itself. However once stacked amongst other plates, I seen trich everywhere from an angle.




Edited by SupaThaRipper (01/21/24 08:14 PM)


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OfflineGogoBonkers
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Re: LAGM v. 2.024 [Re: SupaThaRipper] * 2
    #28602933 - 12/30/23 04:09 AM (29 days, 3 hours ago)

(The guidelines for the grow are now at the bottom of the post, looks better like that)


Allright here we go. I'll always update to the top:



28th January:


Made transfers to four T2 plates with the liberty caps yesterday. Two new plates from each T1.
Had no black plates at hand, just pale ones.
Next batch will probably be black again






21th January:



Healthy grow on the two liberty transfers, shown on the bottom two pics, not ready for next transfer yet.
All three cube plates are still empty, now i really wonder. It's such a bummer and funny at the same time, but i'll let them sit, there might still happen a miracle.





15th January:


today i made transfers of the two liberty plates, still nothing on the cube plates, i smell epic fail :smile:






12th January:


Still not much to see on the cube plates, neither contams. Amazonian i already had six plates refuse starting, so i am not surprised about that one. I guess the low temp these days in here(<65f) slows things a bit down as well. The hopes to get at least one cube going are high.

The mycelial growth on liberty #1 really seems to be the right one, mostly given by the fact it's growing slowly and looks good. Could still be mold. Transfer it soon, see what happens and report of course.
Plate #2 also shows weak myc now and a second kind of yellow bacteria.
For the liberty caps i decided to set off the restrictions i set above. After studying the official semilanceata thread i see that i better have more than just two jars to get something going. But plate#2 i'll still clean up and not just take #1 now.



9th January:

There is some mycelial growth on the clean liberty plate, still not sure if it's the right myc. I'll keep an eye on it and report back. The other plates remain the same.




7th January:

First tiny update, got some white stuff on one of the liberty plates, which were expected to show contams any way. I think it's just bacteria in my eyes, but that's actually a good sign regarding the other plates showing no bacteria. So far long starting off times were normal with any cube mycelium for me, so no reason to get nervous yet. See you in a couple of days.






1st January:

B+ and Amazonian had to be prepared, i have them in vials. So i made the two syringes first, before really starting out.

Showered, stoned, teeth brushed, mask and gloves on, music running, mobile wiped and in flight mode, so actually no reason to stand up again. But no, the clingwrap is still in the kitchen, damnit! :smile:

The inoculations worked pretty ok. I wanted to add a short video, but now i see it's only possible from websites :frown:




a small set of rules for myself (edit 1/12 set off for liberty caps, for several reason):

-Only five plates:
2xLiberty caps
1xPES Amazonian
1xB+
1xMVP
-If plates show no growth they're out
-Plates will be transfered until clean looking and relatively uniform, but always only one receiving plate
-Water agar is an option for heavy bacterial infections, but not the default
-The donor plates for transfers won't be kept
-Only one jar will be inoculated per sending plate, the sending plate will be kept at room temp, hoping for plins and as a backup if the jar contams
-g2g only allowed for clones, plins and their transfers
-If one plate throws plins, jackpot!, every fruit can go into it's own jar
-Only one shake for jars at around 30%
-If jar contams in any way or stalls completely, it's a safe toss and the next jar will be inoculated after transfering at least once from the sending plate
-If a jar (not the liberty caps) makes it to 100% clean, it will be s2b into 5l unmodified shoeboxes with coir or cv in a ratio between 1:2 and 1:3
-If fruits show up, clones are allowed without any limits, g2g unlimited included, but plate rules and restrictions like above
-No clones from clones, only continue from prints after growing out a clone or plin

In theory it is possible to go game over with these rules, but as further it gets as more unlikely it will be. Try best to keep it going.

So far i had two major problems in my grows. First long colonization times, also with clean looking jars. Second rosecomb. I am curious how these problems will affect this project, but as a possible solution to the colonization time issue i am trying wbs these days instead of my normal rye. So i am still not sure which grain i will use exactly. The rosecomb is, if my research is correct, no problem as long as i don't take sporeprints from infected fruits.

Normally i don't use food coloring for plates, but because my agar pictures suck always ass i decided that black dye will make it easier to get relatively good pics.

The agar recipe i use:
500ml dest h2o
4g instant potato flakes
5g dextrose
7g agar agar powder
1g nutritional yeast

See you soon!


Edited by GogoBonkers (01/27/24 11:36 PM)


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: GogoBonkers] * 2
    #28603838 - 12/30/23 08:37 PM (28 days, 11 hours ago)

Quote:

GogoBonkers said:
I hope i got things right, so updates happen by editing THIS post, starting with spores to agar at the 1st of january. I assume it's ok if the plates are prepared before.




Yep, you got it! Just keep editing the post I'm replying to. Plates can definitely be prepped ahead of time you don't need to pour plates on 1/1/24.




Is anyone planning to put spores to agar right at the stroke of midnight? I'll probably get my plates streaked sometime on New Years Day depending how much celebrating I do the night before lol. All my Pan prints are pretty old so if they don't germ I'll have to switch over to some cubes or somethin. Time will tell!


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28603859 - 12/30/23 08:54 PM (28 days, 10 hours ago)

Probably start when I get up on the first, too. Just posted my first update about prepping plates. Also just picked up some new slow subtrop genetics at the mailbox, so just need to decide if I want to add more to the LAGM list or keep the list short.

So if I just got back from Central Europe and I am still jet lagged, can I start at 4pm local tomorrow???


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Re: LAGM v. 2.024 [Re: ReverendMyc]
    #28603888 - 12/30/23 09:19 PM (28 days, 10 hours ago)

Quote:

ReverendMyc said:
Probably start when I get up on the first, too. Just posted my first update about prepping plates. Also just picked up some new slow subtrop genetics at the mailbox, so just need to decide if I want to add more to the LAGM list or keep the list short.

So if I just got back from Central Europe and I am still jet lagged, can I start at 4pm local tomorrow???





Oh you can start literally any time you want so long as it's within 2024. No requirements to start on the 1st. There are always people joining weeks or months late :thumbup:


--------------------



I :heart: 5318008


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OfflineGuyOnaBuffalo
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28603933 - 12/30/23 09:53 PM (28 days, 9 hours ago)

I'll put my hat in the ring. I'll come back and update when I know what I want to start, like a couple others I already have a few peojects underway.


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InvisibleFloret
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Re: LAGM v. 2.024 [Re: GuyOnaBuffalo]
    #28604132 - 12/31/23 04:28 AM (28 days, 3 hours ago)

What's the rules after spores to agar? Strictly monos?  What about muda bottles, LC/LI to PF Tek, water tubs, faht locks, shoeboxes, marthas? SGFC :naughty:?


--------------------
LAGM 2024


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InvisibleMojaveMyc
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Re: LAGM v. 2.024 [Re: Floret] * 2
    #28604198 - 12/31/23 06:20 AM (28 days, 1 hour ago)

Grow any way you’d like to, as long as you’re coming from agar first :rockon:

Gathered my prints & poured my plates. Catch yall when the fun begins tomorrow :dancer:


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Re: LAGM v. 2.024 [Re: MojaveMyc]
    #28604578 - 12/31/23 12:23 PM (27 days, 19 hours ago)

I'm in, LAGM 2024 here I come. Let's see if I make it to the finish.

Mexicana A
Cubensis "gold top", a personal project of themushroombloke

2024-01-06
Prepped no pours, LME .1%

2024-01-07
Prints streaked to plates with sterilized swabs
3 mex, 2 cube

2024-01-10
One mex plate is very bacterial, I can see a wet orange streak where condensation rolled around. This isn't a problem when I let my plates sit for a couple weeks before use. No other activity for the mex.
One cube plate has bacteria, both cube plates have some growth. Now to let them grow out to see if it's myc or mold.

2024-01-14
Still no good activity on the mex.
Took T1 cube transfers, the black spots are ash from the scalpel.


2024-01-21
I gave up on the mex plates taken on the 7th and I streaked two more. This time I ripped off a bit of the swab and shoved it into the agar.
The two cube T1s looked clean enough to send to grain so I transferred each plate to its own half pint of oats. I also took another transfer as backup.



--------------------
LAGM 2024


Edited by Floret (01/22/24 02:40 AM)


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OfflineAndImTheHighOne
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Re: LAGM v. 2.024 [Re: Floret] * 1
    #28605306 - 12/31/23 11:44 PM (27 days, 7 hours ago)

I was maybe going to start tonight, but I'm not really in shape to be doing any sterile work at the moment. Will have to wait til morning lol!


--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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OnlineEniQma
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Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28605310 - 12/31/23 11:55 PM (27 days, 7 hours ago)

Quote:

AndImTheHighOne said:
I was maybe going to start tonight, but I'm not really in shape to be doing any sterile work at the moment. Will have to wait til morning lol!



Same pretty drunk atm


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OfflinePnin
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Re: LAGM v. 2.024 [Re: EniQma]
    #28605561 - 01/01/24 08:53 AM (26 days, 22 hours ago)

OP updated!
:kissit:


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
>my end of 2023 grow journal<


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OfflineVibetyme
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Re: LAGM v. 2.024 [Re: Pnin] * 2
    #28605737 - 01/01/24 11:51 AM (26 days, 19 hours ago)

Vibetyme's  LAGM 2024

Albino Riptide

Starry Night APE

Toque


1/10  My plates are finally nocced up. I dipped my swabs in  sterile water to hopefully knock off some surface contams and hydrate the spores. Then I stuck the tip down in the surface of first cup in five places, on the second cup I just stabbed it in and called it good. I'm stoked!





We have lift off on all them thangs! Super stoked about these varieties! 




Edited by Vibetyme (01/22/24 01:15 AM)


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OnlineEniQma
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Re: LAGM v. 2.024 [Re: EniQma] * 3
    #28605803 - 01/01/24 12:44 PM (26 days, 18 hours ago)

Quote:

EniQma said:
Ok, so I’m going to make this the post I add to right?

I will be starting germ plates on 1/1
I’ll be doing no pours in small recycled glass jars with polyfill filters.

I have these to choose from, maybe someone could help me pick 3 since I already have a number of projects underway.

Jack Frost
Ghost
Wild Florida Cube
Natalensis
Pan Jambo
Thai Lipa Yai
Hillbilly
Pan BVI
GWM
Ps. Cyanescens
Wild ovoids I found

Seriously though, my interest is yours so lmk which 3 I should do. I wanna see some diversity around here!

I hope this isn’t going to get me disqualified!


So it’s 12/28/23 and I got my agar cooked.

I usually just wing it but this time I made a mental note to share with you fine folks. Could be soft, could be hard, time will tell…
I brought 250g water (ghetto I know) to a boil and slowly added 2.5g agar agar powder and 2.5g brown rice flour while whisking with a fork.


That was enough to make 9 of these plates(actually jars) and make them thicc. Just the way I like it 😉
Too bad I could only fit 8 in my presto

Prompting me to do a second batch.

I let the cooker vent for 10 min without the weight. Timer begins when the stopcock?(I think it’s called) was fully erect.

And once reaching 15psi I cooked for 20min

And two pro tips for any noobies,
1. Take them out hot to solidify on a flat surface.
2. Use a piece of paper towel between polyfill and foil to prevent issues with bacteria(that could occur prior to inoculation)


It’s 1/1/24 Happy New Year!
To kick it off I chose…
1. Natalensis
2. Ghost
3. Wild Ovoid

First I let my filter run 10 min. I keep it covered so nothing settles on the surface anyway, but it’s just a precautionary measure.

Then I wipe the desk and grates down with 70% iso


I proceed to do the same with my jars making sure to get up under the rim of the lid with the paper towel


Then the prints come out, and instead of using loop I just use my sterilized qtips.


It’s hard to take pictures while doing the work because I like to be fast and thorough, but I keep them as close to the flow as possible without touching and never cross anything in the path of flow from the fan to the media.




Edited by EniQma (01/01/24 03:04 PM)


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: EniQma]
    #28605830 - 01/01/24 01:00 PM (26 days, 18 hours ago)

Very nice setup! I'm still scraping by in a SAB but it gets the job done. I doubt I'll get around to streaking any plates today but time will tell


--------------------



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InvisibleMojaveMyc
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28605862 - 01/01/24 01:27 PM (26 days, 18 hours ago)

First (tiny) update is in! Happy New Year mush fam! I’m looking forward to growing along with you guys :mushroom2:


--------------------


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OfflineAndImTheHighOne
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Re: LAGM v. 2.024 [Re: MojaveMyc]
    #28605871 - 01/01/24 01:34 PM (26 days, 18 hours ago)

Updated! Still very much a learning experience with this, so you'll have to excuse the lack of pictures and details of my actual processes initially. I'm still using my phone to essentially read TEKs as I go, and I'm not nearly as bad ass as rawdog-countertop-guy, so most of my pics will be of finished products.


--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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InvisibleReverendMyc

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Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28606088 - 01/01/24 04:15 PM (26 days, 15 hours ago)

Welcome to the grow along. The more you share about your process and pictures that you include, the more the fine folks around here will be willing to help or learn from your methods, miracles and mistakes. That is kind of what this thread is all about.

I have updated my OP with preliminary plating of the planned prints and added a some subgenre of subtropicalis.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Offlinemnj
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Re: LAGM v. 2.024 [Re: AndImTheHighOne] * 3
    #28606097 - 01/01/24 04:29 PM (26 days, 15 hours ago)

Hey Y'all, I'm in


Gonna give pans a good effort this year in addition to the usual cubey cubes.

I'd like to plan some small outdoor runs in pots and might tack on some novelty grows for funsies.

Gonna work with what I have on hand, but maybe more if I get the inspiration. Who's to say?

Penis Envy  abandoned for LAGM due to weak/weird growth on germ and t1 plates
Albino A+
Pan Cambo Thailand Suphanburi Goliath
Pan Cyan Hawaii

Let's boogie :awedance:


Update    Update


January 2, 2024

Got 'em streaked and labeled just before midnight.  Gotta love havin plates and syringes in the closet.

Gear: SAB, plates, syringes, inoc loop, torch, scotch, tunes



Check out that time stamp on the stove :rockon:




Update    Update


January 7, 2024

Hey y'all

I've been enjoying the spirit of LAGM and seeing everyone's updates.

When I started a week ago I overlooked the fact that one of the inspirations for LAGM was to demystify the process for newcomers, so from here on out I will include details and attach links when I can.  I've never written down what I do so I guess this will also serve as my personal reference for whenever I come back from taking breaks.  Everything I do has been gleaned from other members and so many awesome threads



My Agar Recipe:

The plates I used were pastywhyte's pasty plates using Pastywhyte's Easy Agar Tek.  Here's the recipe I had in my phone notes from 6 years ago.  I don't know how true this recipe is to the original but it works for me and I do it.  I think it makes about 6-8 pasty plates depending on the container I use (idk, it's been months since I've made it):

  • 1.5 - 1.75 grams agar powder (I stay on the lower end for softer agar, better for germination i think?)
  • 1.5 - 2 teaspoons instant potato flakes (no flavoring you freaks) (I stay on the lower end or even cut the amount in half if I want bolder/more-vigorous growth because I think I read that's a thing?)
  • 1/2 cup water
  • Drop of honey
  • ~3 drops of food coloring (I like blue) (apparently red food coloring is anti-fungal?! Might be an urban legend but the last time I used red things didn't go so well...coincidence?...readjusts tin foil hat) 

Recipe is easily doubled, just use twice as much of each ingredient.


My containers:

Lately, because I think Glad stopped making the 4 oz round containers (and the ones I ordered off amazon must have sat in a hot warehouse for years because none of the lids fit), I've been using wide mouth half-pint jars with a plastic (no rust!) Ball brand black/grey plastic lid with a 1/8" - 1/4" hole drilled in it (or whatever size my Dremel bit is; a hot nail will do just fine), covered with micropore tape (I'm sure polyfil or other alternative will work).  The high and straight walls of the jar are not ideal but I've been managing just fine in my still-air-box, just have find a good angle so I minimize the amount that the unsterile handle of my scalpel/inoculation loop hovers over the surface).


How I prep the agar for plates:

I add the water to an egg sauté pan (I like the low/angled walls for whisking) and add potato flakes, let 'em cook a few minutes to incorporate (keep it at a simmer so the water level doesn't evaporate too much, i try to keep the water to agar ratio pretty stable) then add drop of honey and then agar powder a little at a time, stirring with a whisk so it doesn't clump, finally i finish with the food coloring.  I spoon it into my containers a tablespoon at a time (the larger spoon in your spoon drawer) and then I add the remaining mixture by the spoonful or half-spoonful, depending on what's left in the pan, jar-by-jar until the depth looks good (some containers have a rise in the bottom-middle that I try to overshoot). 

Finish by putting on container lids, fold up half-a-paper-towel into a square and put it on the lid, then wrap the whole thing in foil.  I fold and roll the excess foil to the sides of the container, keeping the bottom as flat as possible, because that means the agar disc will be as level as possible when it cools after the pc cycle.


Prepping the PC

I prep the pc by loosely crumpling up foil and lining the bottom of the pc so that my trivet will sit several inches off the bottom, then add 1.5" - 2" of water, and put the trivet on top of the foil.  This helps keep the plates away from the boiling water bubbles.  Load the plates and put lid on pc.

I vent the pc for 10 mins then I put on the weight.  Once the pressure rises to 15 psi, I pc for 45 mins, then turn the burner off and allow the pc to return to 0 psi naturally.


How I store my containers:

This I learned from Josex in his Josex' Guide to No-pour Agar tek: After pc reaches 0 psi, I take the plates out of the pc while still hot and let them settle on a flat surface so the agar disk (hopefully) solidifies with a flat surface (in a short long rectangular plastic tote with a lid where they live in my closet until I need to use them.  I put the lid on the tote after they cool so condensation doesn't build up inside the tote.  A few paper towels laid in the tote will soak up ambient moisture.)

The plates I used to swipe spores on January 1 had been stored for several months.  The benefit of this is they had no condensation on the sides!  My visibility with no-pour plates was greatly improved!!!  Edit: these plates had better visibility but they were also dry as hell and not great for germination.


How I Inoculate:

For the inoculation process I used a collection of inoc loop info that I gathered and internalized from reading/searching on here, but  BOD's DIY Inoculation Loop TEK is a great place to start.  One tip I picked up from other members is to cool your hot and freshly-flamed loop in the agar of your receiving dish before shooting/swiping spores onto the loop.  A hot loop can cook and minimize the germination ability of your spores.  I was in a hurry and jammin tunes and drinkin scotch when I swiped plates on the 1st so I forgot this step, but I made two plates of each variety and I only sterilized my loop between varieties, so at least the second plate received a streak with a cooled-down loop :thumbsup: Edit: For the second round of plates I just dropped the spore solution directly from the syringe onto the agar


A few days ago I believed I saw germination (is that a white clump starting to form or is that just the light reflecting off a small indentation left by the inoc loop?!?)

Today I for-sure have germination on two plates.  The small white dots in the photos are dried agar/potato flake residue on the walls of the container from where the mix boiled up during the pc process.  The denser, bigger, whiter dots are spore germination :smile:


AA+




Pan Cyan Hawaii



:cheers:


Update    Update


January 11, 2024

Seeing some germination on a Penis Envy plate



Half my plates have growth but all growth is happening very slowly.  I think it's a combination of cold temps (low-mid 60s) and using old plates that are kinda dry. 

I made fresh plates a few days ago so I will noc some up this weekend (my pan cambo has not germinated and I wanna give that another go, plus I'd like to get more diversity with AA+) and I may do some transfers if the myc has moved a significant distance from the germination points (it's all going very slowly)


Update    Update


January 14, 2024

Woke up nice and early so I cleaned off the kitchen counter and brought out the SAB. 

Shot some of the pasty plates I made last weekend with mss of Pan Cambo Thai and AA+, two plates of each. 

Made T1 transfers of Penis Envy and AA+.  I wasn't impressed with either variety's germ plate, but thought I'd make the transfers and see what happens.

No pics of these plates because the condensation is ridiculous.

My Pan Cyan Hawaii germ plate is coming along with several germination points.  One has spread far enough for transfers but the other two are not yet ready so I'm gonna let it ride a little longer:



Gonna cook more plates today so I'll be ready for transfers whenever the donor plates are ready


Update    Update


January 18, 2024

My original AA+ plate had only germinated in one spot as a bright white fuzzy mass that reached as high as it did wide. Took transfers to a few plates anyway to see what was up (because the Oak Ridge I did last year also started out growing very vertical on the germ plates but performed super well!). Anyway, the two T1 plates are growing fast and fuzzy and I don't trust em. Today I put em in a ziploc and then a Tupperware and set em aside to see what happens. Either I've got two dope mold plates or I'll have plins in mid February. My gut says mold.

I've already noc'd up a few fresh AA+ plates bc I've never grown this variety and wanna give it a go.  Germination is happening on both so looking forward to making transfers soon.

I ditched my PE because it was a translucent top crawler and overall the agar it touched looked gross. Idk wtf it was but it wasn't worth my time.  It looked like myc in its growth patterns but was only visible from the way it reflected under a flashlight.  I already have a few PE clone cultures going from 2023 so I will use my time and space to focus on those. Might do some top fruited cakes if I want more options but that won't be part of this thread.


Update    Update


January 24, 2024

Did some T1's today of Pan Cambo, Pan Cyan and AA+.  Took transfers to multiple plates.  Some of the transfers came off the xacto knife clean and others I had to wiggle and waggle off the blade :shrug:

Here's a pic of some of them .  Germ plates on bottom, T1's on top.



You can see some non-LAGM cakes in the background that I shot with mss of Pan Cambo and Pan Cyan during the same SAB session.  I always feel like I'm missing out on a good culture when I make agar selections so I plan to top-fruit these cakes to look for clone candidates. 

Anyway, I know this isn't technically LAGM, but thanks to the LAGM process I know these two spore syringes are what I would call "clean" because they have not shown signs of contamination on agar, so I thought I'd go for it. :thumbup:

Cakes were made of store-bought black kow manure + oats + verm and pc'd 90 mins.

I was inspired by the following wonderful threads: V.L's EF-tek (Exotics Fanaticus cakes), MacMerdin's MacMerdin's Simple Pan-Cakes, MudaFuka's Updated Bottle Tek New and improved, and Bod's PF tek for cloning on the EZ.


Edited by mnj (01/24/24 08:47 AM)


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OfflineSeventhMushroom
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Registered: 12/30/22
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Re: LAGM v. 2.024 [Re: mnj] * 2
    #28606139 - 01/01/24 05:07 PM (26 days, 14 hours ago)

Updated post. Got to use my new flowhood, got to use a spore print for the first time, got some gourmet cultures started in jars while I was at it. Let's hope for some mushrooms :laugh:



--------------------
LAGM 2024


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OfflineGogoBonkers
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Re: LAGM v. 2.024 [Re: SeventhMushroom]
    #28606268 - 01/01/24 06:29 PM (26 days, 13 hours ago)

Updated Op

Happy new year 2024!


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OfflineNoncense
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Re: LAGM v. 2.024 [Re: GogoBonkers]
    #28606287 - 01/01/24 06:40 PM (26 days, 13 hours ago)

Main post updated, sorry it took me a few days just wrapping up my year and have been under the weather and busy myself. As a reminder, the rules are in the main body of the post, they are very literal. Spores to agar, after that go hog wild with your grow method.

Thanks everyone for participating, ill be claiming my own spot shortly.


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OfflineNoncense
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Re: LAGM v. 2.024 [Re: Noncense] * 2
    #28606311 - 01/01/24 06:53 PM (26 days, 12 hours ago)

Noncense LAGM 2024


MENU
Cubensis Vars.
TD
Steel Mag 5
Albino Thrape #1 (Nug) F1
A. Thrape #2 (FC) F1
A. Thrape #3 (LB) F1
MORE TBD
Exotics:
Ps. Semperviva

1/1/24
Spores swabs to agar standard 10 grams lme 10 grams agar recipe, green and blue color for funsies.


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OfflineWomble
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Re: LAGM v. 2.024 [Re: Noncense] * 1
    #28606602 - 01/02/24 02:59 AM (26 days, 4 hours ago)

I'm in, as luck would have it I recently pored my first agar plates ever and streaked a bunch of them on new years day. So this has been timed perfectly to participate in this.

I'll be trying to grow:
MVP
Redboy
GT

30/12/2023
Poured my first plates I have ever poured today. Recipe was 500ml water, 10g of Telephone brand Agar Agar powder and 10g of light malt extract powder. Followed guides on here and vented PC for 10 mins and PC'd for 20 mins. Allowed PC to depressurize after the cycle and allowed agar to cool to around 48c and poured my plates - got 19 in total.



01/01/2024
Smeared 10 plates in zig zag pattern with 5 varieties using my DIY inoculation loop (only 3 varieties as part of this LAGM). 2 plates per variety for some sort of redundancy.

Both MVP and Redboy spores were donated to me from a generous member in the marketplace and the GT spores came from my personal prints. The other 4 plates also came from my personal prints but I cannot be sure exactly what they are so i'm not including them as part of this.



03/01/2024
No noticeable growth on any plates - hoping that my agar mix was not too firm for a germination plate - fingers crossed they are successful as the MVP and Redboy prints I received were very light and I may only have one more attempt using them.

05/01/2024
I have visible germination on 5 out of 6 plates so far. Stoked about the MVP and Redboy as they are both new genetics I received. Will try and play around with lighting to get some decent pictures over the next few days.

Germination:
GT - 1 of 2
MVP - 2 of 2
Redboy - 2 of 2

08/01/2024
Germination pics, apologies for the picture quality but plates are hard to take photo's of any tips from anyone?:

MVP - both plates look like this:


Redboy - both plates look like this:


GT - the 1 plate where I thought I saw germination had heavy condensation and so I put a hot cup of water on the plate, it looks like a lot of condensation got washed around the plate and looks heavily bacterial?

I think I will have to pour some new plates and start these again from spores or chalk these up as a failure and just run with the other 2 strains.




Edited by Womble (01/08/24 03:56 AM)


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OfflineSirPsycho
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Re: LAGM v. 2.024 [Re: Noncense] * 6
    #28606727 - 01/02/24 07:45 AM (25 days, 23 hours ago)

I was planning to just lurk in the shadows but non still hasn't update the prizes so I'll just tell you funguys and fungirls.

I've put up 5 Ps tampanesis (F1 off a wild South Carolina find), 3 Ps subtropicalis/semperviva and 3 cubensis var ESS prints.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineMonkeyJesusFresco
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Re: LAGM v. 2.024 [Re: SirPsycho] * 2
    #28607867 - 01/02/24 11:45 PM (25 days, 7 hours ago)

ah what the hell, I'm in :shrug:

tba

guess I'll give it a go :shrug:


--------------------
LAGM v 2.024
- endo cabendo


Edited by MonkeyJesusFresco (01/05/24 01:38 AM)


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OfflineSirPsycho
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Re: LAGM v. 2.024 [Re: MonkeyJesusFresco] * 7
    #28608236 - 01/03/24 09:56 AM (24 days, 21 hours ago)

Ok, I guess I'll grow some mushrooms. This is a wild print sent to me by a buddy in Florida. Found in 2022



--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: SirPsycho]
    #28608958 - 01/03/24 09:28 PM (24 days, 10 hours ago)

Very minor update with no pictures. Got Pan. cyan BVI, Bispo, NecD, Wild Coast and Jambo all streaked..and now we wait!


--------------------



I :heart: 5318008


NOT a virgin!


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OnlineEniQma
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28608960 - 01/03/24 09:30 PM (24 days, 10 hours ago)

:creepylurker:


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OfflineTri-Polar
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Re: LAGM v. 2.024 [Re: EniQma] * 2
    #28608967 - 01/03/24 09:36 PM (24 days, 10 hours ago)

Dammit im in, maybe this will finally be the year I actually stay consistent with documenting my LAGM

I will be growing some BAPER-R

January 7th
Put spores to agar, first time using an inoculation loop so I feel like I fucked it up, also was too busy to pour new plates so I used an old PDA plate from a batch I was pretty displeased with but I couldnt care less and probably neither could the spores so here we go babyyy


January 10th
Put more spores to agar this time with a swab, really hit me in that moment- you can make metric fuck tons of swabs from a single print if you're so inclined, neat.



January 17th

good news we got germination, bad news im sick af with covid

Win some lose some


Edited by Tri-Polar (01/17/24 01:48 PM)


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OfflineMonkeyJesusFresco
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Re: LAGM v. 2.024 [Re: Tri-Polar]
    #28609022 - 01/03/24 11:38 PM (24 days, 8 hours ago)

what's BAPER-R?


--------------------
LAGM v 2.024
- endo cabendo


Edited by MonkeyJesusFresco (01/03/24 11:38 PM)


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OnlineEniQma
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Re: LAGM v. 2.024 [Re: MonkeyJesusFresco] * 1
    #28609123 - 01/04/24 05:32 AM (24 days, 2 hours ago)

Black ape revert redspore….
Duh 🤦‍♂️


Edited by EniQma (01/04/24 06:25 AM)


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: EniQma] * 3
    #28609238 - 01/04/24 07:54 AM (23 days, 23 hours ago)

Well just as anyone would have guessed there is no action on any of my germ plates which were inoculated less than 12 hours ago :lol: the compulsive observations of my projects have officially begun!


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InvisibleMojaveMyc
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28609280 - 01/04/24 08:48 AM (23 days, 22 hours ago)

Hahahah same energy here. I’m finding it fun to be back in that mindset. Fingers crossed you see some action soon 🤞🏾

OP updated


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OfflineRoscoeReturnsS
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 3
    #28609353 - 01/04/24 10:35 AM (23 days, 21 hours ago)

Quote:

bigfootscreepyuncl said:
Well just as anyone would have guessed there is no action on any of my germ plates which were inoculated less than 12 hours ago :lol: the compulsive observations of my projects have officially begun!




Pics or it didn’t didn’t happen.


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OfflineGastronomicus
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Re: LAGM v. 2.024 [Re: RoscoeReturns] * 1
    #28609373 - 01/04/24 11:00 AM (23 days, 20 hours ago)

Day after Update


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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InvisibleReverendMyc

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Re: LAGM v. 2.024 [Re: Gastronomicus] * 2
    #28609459 - 01/04/24 12:57 PM (23 days, 18 hours ago)

OP updated with the following:
Got some action on Pan Bunnell and the Subtrop Bell Cap!
Not all good. Bacterial brain blob.


But some good myc on both pan bunnell plates.


And stoked to see the subtrop bell cap already germing on one plate.


There might be some action on some of the rest, but too early to be certain.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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OfflineCaptainPuffy
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Re: LAGM v. 2.024 [Re: ReverendMyc] * 1
    #28609583 - 01/04/24 03:36 PM (23 days, 16 hours ago)

This is serving as my grow log for the entry:

I am starting to see growth on my swabbed plates.

I have APE-R, PE6, and Pan Cyan BVI





Update 1/14

I forgot to take pictures, but I made my first transfers to home made agar.

My Pan Cyan mycelium turned yellow, so definitely dealing with some kind of bacterial infection. I took transfers from the outside edge as small as possible. I'm going to let those grow for a week and see how they look. I ordered additional Pan Cyan spores just in case I can't clean these up.

My PE6 and APE-R both looked nice. I made 4 transfers each from 5 plates.




Update 1/21

I usually shower immediately before doing my SAB work, but had a busy day so I only showered in the morning. It definitely shows in my agar work.

My clean procedure is this:
-Shower
-Brush Teeth and use mouth wash
-Put on clean clothes
-Wash hands with soap and water
-Put on Gloves and Mask
-Wipe inside of SAB with soap and water to clean
-Wet sides of SAB with soapy water
-Spray inside with lysol spray
-Let sit for 2 minutes
-Sanitize gloves with 70% Iso

APE-R did the worst, I didn't end up with a single sample that is 100% clean. There is still enough healthy mycelium to make another round of transfers. I'm going to take from the nicest growing ones and discard the rest.



PE6 did OK, some of the plates were extremely bacterial, but I did end up with one 100% clean plate. I plan to make a LC from that plate, and make multiple transfers to expand my culture collection.



Pan Cyan is the one I have the highest hopes for. All the cultures look really good with very little bacterial contamination. I'm going to make a LC using the whispyiest one, and transfer the rest to multiple plates.



All in all, I've learned that my clean procedure needs work. If anyone has any recommendations I'm all ears.


Edited by CaptainPuffy (01/21/24 07:57 PM)


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: CaptainPuffy]
    #28609606 - 01/04/24 03:53 PM (23 days, 15 hours ago)

Quote:

CaptainPuffy said:
Can I get in on this? I just put spores to agar a few days ago

I have APE-R, PE6, and I'm trying Pan Cyan

I'm also trying my hand at liberty caps and what lovers but I don't have the spores yet





Hell yeah you can get in on this action!


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28609685 - 01/04/24 05:01 PM (23 days, 14 hours ago)

Alright I decided to go with Albino Riptide, Starry Night Ape and Toque.

Mostly because they all they look cool as hell. 

I only have one swab of each, so some luck might be required.


--------------------
Vibetyme's LAGM 2.022

Vibetyme's LAGM_23

Vibetyme's LAGM_24

                 


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Re: LAGM v. 2.024 [Re: ReverendMyc] * 1
    #28609705 - 01/04/24 05:13 PM (23 days, 14 hours ago)

Good luck mate :cheers:


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
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Re: LAGM v. 2.024 [Re: mnj] * 1
    #28609826 - 01/04/24 07:04 PM (23 days, 12 hours ago)

Updated my post. Seeing some type of growth. I think it's good but I honestly don't have a clue yet lol



--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: Vibetyme] * 1
    #28609836 - 01/04/24 07:13 PM (23 days, 12 hours ago)

Quote:

Vibetyme said:
Alright I decided to go with Albino Riptide, Starry Night Ape and Toque.

Mostly because they all they look cool as hell. 

I only have one swab of each, so some luck might be required.





Starry Night you say? I wish you all the best luck Vibe! Because once you get a flush I'm gonna hit you with that :begger:    :lol:

I'm not sure but I might have an albino riptide swab I'd be willing to share with you if yours doesn't work out..either that or an albino tidal wave. IDK it's been a long time since I've gone through my cube spores lol


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28609874 - 01/04/24 07:52 PM (23 days, 11 hours ago)

It's a SN swab and while I hope for the best I honestly think it's gonna be hard getting more then one or two actual SN fruits coming from spore.

my apes have always been shit but I keep trying.

If I do get something cool you already know I'll definitely share with you homie. I'll hit you up if need be on that swab. Thanks for the offer!


--------------------
Vibetyme's LAGM 2.022

Vibetyme's LAGM_23

Vibetyme's LAGM_24

                 


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Re: LAGM v. 2.024 [Re: Vibetyme]
    #28609906 - 01/04/24 08:19 PM (23 days, 11 hours ago)

I just heard about this... Too late to throw my hat in?


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Re: LAGM v. 2.024 [Re: mycosispeon] * 2
    #28609921 - 01/04/24 08:39 PM (23 days, 11 hours ago)

Nope! There will be people joining over the next month or two. That's the beauty of LAGM, you can't be too late, only too early lol


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28609938 - 01/04/24 09:05 PM (23 days, 10 hours ago)

Quote:

bigfootscreepyuncl said:
Nope! There will be people joining over the next month or two. That's the beauty of LAGM, you can't be too late, only too early lol



Okay. I was hoping to obtain some better genetics, but I DID inoculate 4 dishes on 1/1.

x2 JMF, MSS syringe (syringe is sus, I'm honestly not hopeful)
x2 Enigma, LC syringe (not from spores so maybe doesn't qualify?)
(hopefully one or two more to come)


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28610145 - 01/05/24 05:32 AM (23 days, 2 hours ago)

Let's gooooooo

First time growing both of these so let's see how it goes
1. Ps. Cubensis - Na Mueng
2. Pan. Cyanescens - FL Red Spore F3

Method: Spore -> Agar -> LC -> Grain -> Bulk



Edited by stockw (01/07/24 06:22 AM)


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Re: LAGM v. 2.024 [Re: stockw] * 2
    #28610334 - 01/05/24 10:48 AM (22 days, 20 hours ago)

Still nothing to report on....Maybe if I check on my plates more frequently they'll germinate faster!


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InvisibleReverendMyc

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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 3
    #28610383 - 01/05/24 11:21 AM (22 days, 20 hours ago)

I know that you are joking, but it really works. You probably just aren't checking often enough. :shrug:
Try blinking less.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Offlineedixo
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Re: LAGM v. 2.024 [Re: ReverendMyc]
    #28610388 - 01/05/24 11:24 AM (22 days, 20 hours ago)

Imma have to go check my plates again


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~ LAGM 2024 ~


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: ReverendMyc]
    #28610405 - 01/05/24 11:36 AM (22 days, 20 hours ago)

Quote:

ReverendMyc said:Try blinking less.




:kittylaugh:


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OfflineWomble
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28610418 - 01/05/24 11:50 AM (22 days, 19 hours ago)

Personally, I'm only taking my eyes off of my plates to check this thread and then its straight back to turning and moving them around using different lights and my phone to see if there's been any germination or further growth.

On a serious note, happy to report I do have some germination.

Also not so happy top report I think I might also have the threads first mold germination, there's an award/prize for that too right? :laugh2:


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OfflineCaptainPuffy
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Re: LAGM v. 2.024 [Re: Womble] * 2
    #28610424 - 01/05/24 11:55 AM (22 days, 19 hours ago)

I drive truck and am gone for a week at a time. I bug my wife to send me pictures


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Re: LAGM v. 2.024 [Re: CaptainPuffy]
    #28610487 - 01/05/24 12:46 PM (22 days, 18 hours ago)

Hi everyone, count me in.

Strains:
-Shakti
-Yellow Umbo x Great Monster F2 (YUxGM)
-Black cap natalensis (post in transit, thank you SupaThaRipper🤗)

I have one question. I put YUxGM spores to agar few days before 1.1., should i start with new swab to agar or I'm allowed to use few days older culture?
Other strains will be put on agar soon.

My SAB, looks lame with foil and ducktape, but works.


Everytime I work in SAB, I open agar plate to check cleanliness of air. That is 3rd time and still no contaminations.



6/1/24
Shakti spores put on agar





8/1/24
YUxGM spores on agar.



11/1/24
Signs of germination from both strains (Shakti and YUxGM) not fluffy white mycelium, but visible differences on top face of agar.

27/1/24

Shakti, YUxGM T1 transfer today.



Edited by r0kovski (01/27/24 11:21 AM)


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Offlinemycosispeon
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Re: LAGM v. 2.024 [Re: r0kovski] * 1
    #28610566 - 01/05/24 02:07 PM (22 days, 17 hours ago)

:mushroom2::mushroom2: This post will serve as my Official Entry and Grow Log :mushroom2::mushroom2:

I'll be attempting a few different P. Cubensis subspecies:

  • JMF from MSS (sus)
  • TAT from Swab
  • Ghost from Swab


12/09/23

Poured agar dishes

I decided to do a 56/44 MEA mixture. My media bottles are 500ml, so the recipe was split in two batches.

1000ml distilled water
20g Telephone agar-agar
16g Light dry malt extract

Water heated in microwave; mixed with combined powder; PC for 20min @ 15psi; water bath to 120°F; poured in SAB, bottom-up in stacks of 10 (came out with 48); allowed to cool, and wrapped with cling wrap

01/01/24

Inoculated Agar

I've been having a hard time getting a good culture out of a suspect syringe I received a few weeks ago, so I decided to try one more time. In my SAB, I dispensed ~10 drops of solution directly onto the the surface of two agar dishes. The dishes were re-wrapped, labeled (D-106, D-107), and stored. In the past, I've just dumped all of the solution in one spot, but this time, I decided to put one drop in ~10 different spots.

01/05/24

Observations

I had a bitch of a time figuring out how to take images that looked like what I was seeing with my eye. With light behind? With light above? My labels didn't help matters. My camera clearly sucks balls. Here's what I ended up with:



Already there is cause for alarm... I see multiple points of germination, but also some kind of wet bacteria I think. Are these tossers?

01/09/24

Observations

Okay, I'm officially giving up on these dishes.



Spores coming in the mail compliments of bigfootscreepyuncl, I will have some new entries tonight.

01/09/24 (part DOH!)

Inoculated Agar

The disappointment of having to toss the JMF dishes was replaced with the excitement of receiving new genetics (a sincerely hardy thanks to bigfootscreepyuncl!!). So I setup the SAB and went to work. I decided to inoculate two plates each with spore swabs of both TAT and Ghost. It ended up being a bit of a hack job as I had a difficult time extracting fibers from the swab using my tweezers for the first in each set of dishes, but... mission accomplished (and :fingerscrossed:).

TAT

Ghost


01/12/24

Observations

So far, nothing on the dishes I inoculated with TAT. :frown: Fortunately, I've got germination on one of the dishes of Ghost. :smile:

TAT

Ghost


01/15/24

Observations

Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.

TAT

Ghost


01/17/24

Observations

The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.

TAT

Ghost


01/18/24

Isolation

T1s taken! Though all four of my germ plates have at least two different organisms growing on them, only the two that received the swab heads germinated good candidates for isolation. The TAT one only had a single sector that looked good, so I transferred it to a new dish. The Ghost had 3 or 4 interesting sectors, but all of them appeared to have another mold growing right behind and on top of the Cubensis mycelium, so I selected the two with the greatest margin and transferred to a new dish.

TAT

Ghost


01/22/24

Observations

T1s looking good so far. Will definitely have to do a T2 in the coming days. The germ plates really took off since.

TAT

Ghost


01/24/24

Isolation

The T1 dish of Ghost I made on the 18th had grown out to where the two original samples had merged and there were several great looking sectors, so I decided to make T2s. I decided to transfer samples into one new MEA agar dish with yeast added and into one without. Y'know... for science and stuff. The TAT T1 has been lagging behind a bit and like I'd posted in the AGAR thread, the germ plate for that one actually grew out to exhibit some fantastic looking rhizomorphic growth, so I decided to take another round of T1's from it. Again, I transferred a sample both to a dish with, and a dish without yeast added.

No pictures because condensation in the dishes made it difficult. They should clear up in a day or so of sitting upside-down at which point I'll post some pix.

01/27/24

Observations

The new T1s of TAT are really taking off. The original T1 of TAT has one "cord" that is breaking away from the rest. Is that just a rogue strain? T2s of Ghost are looking good. You can really see the difference in agar between the one with vs without yeast.

TAT

Ghost


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



Edited by mycosispeon (01/27/24 11:49 AM)


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28610579 - 01/05/24 02:22 PM (22 days, 17 hours ago)

I wouldn't use any of those. Sorry homie. You can clean bacterial cultures but it can be a painstaking, long process. I usually only do that to myself if it's a rare variety or a clone culture that is worth all the work.

Personally, I'd just start fresh with whatever the mailman drops off next week. Or, if you hate the thought of wasting syringes I'd give the josex poke method a try. I've cleaned up a couple highly bacterial syringes that way...it is such an ingenious TEK


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Offlinemycosispeon
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28610644 - 01/05/24 03:04 PM (22 days, 16 hours ago)

Quote:

bigfootscreepyuncl said:
I wouldn't use any of those. Sorry homie. You can clean bacterial cultures but it can be a painstaking, long process. I usually only do that to myself if it's a rare variety or a clone culture that is worth all the work.

Personally, I'd just start fresh with whatever the mailman drops off next week. Or, if you hate the thought of wasting syringes I'd give the josex poke method a try. I've cleaned up a couple highly bacterial syringes that way...it is such an ingenious TEK




It's all good, I'm not married to 'em. At least I know now that I can toss the suspect syringe. I can (can't) wait for the mailman to come :smirk:


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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OnlineRockinRobot
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28610698 - 01/05/24 03:40 PM (22 days, 16 hours ago)

Updated OP


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: ReverendMyc] * 1
    #28610879 - 01/05/24 06:31 PM (22 days, 13 hours ago)

Quote:

ReverendMyc said:Try blinking less




Holy shit it worked! We have liftoff on my bisporous plate and a minor update made to my grow-log. I'll try and post pictures tomorrow. It's just the faintest of growth and doesn't show up in any pictures, but it's there!

I'm going to need to pour some new plates soon!


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OfflinePnin
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28611342 - 01/06/24 07:14 AM (22 days, 25 minutes ago)

OP updated with some pics of plates. :fedora:


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
>my end of 2023 grow journal<


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: Pnin]
    #28611479 - 01/06/24 09:35 AM (21 days, 22 hours ago)

Very minor update just to add these germination pics of my bispo:



Not the prettiest pics or plates..I do love using yeast in my plates but damn I also miss having crystal clear plates :lol:


Also, I'm pretty certain my eyes are just playing tricks on me but I think I might have some action on my BVI plate


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Offliner0kovski
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28611524 - 01/06/24 10:08 AM (21 days, 21 hours ago)



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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: r0kovski]
    #28612296 - 01/06/24 08:30 PM (21 days, 11 hours ago)

I'm still not 100% sure but I believe my BVI has also sprouted and Wild Coast may be showing signs of life as well. Still nothing on NecD :frown:



I know it's only been 6 days but has anyone made a transfer yet? I'm getting EXCITED! :lol:


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OnlineEniQma
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28612317 - 01/06/24 08:40 PM (21 days, 11 hours ago)

No germination over here yet


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: EniQma]
    #28612817 - 01/07/24 09:51 AM (20 days, 21 hours ago)

Nothing worthy of a full update yet but I have confirmed germination of: Bispo, BVI and Wild Coast. I'm still hopeful but I believe my Jambo and NecD prints are no longer viable. Fingers crossed!

Unless things really start growing quickly it looks like I'll be making my first transfers next weekend


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Offlinemnj
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28612986 - 01/07/24 12:38 PM (20 days, 19 hours ago)

Hold out hope creepyuncl, it's only been a handful of days

Updated me log with methods + links + a few germ pics


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mnj] * 2
    #28613286 - 01/07/24 05:40 PM (20 days, 14 hours ago)

Minor update to add some pics. Confirmed germination on BVI, Wild Coast and Bispo is growing in nicely.



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OfflineCaptainPuffy
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28613350 - 01/07/24 06:58 PM (20 days, 12 hours ago)



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OfflineWomble
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Re: LAGM v. 2.024 [Re: CaptainPuffy]
    #28613603 - 01/08/24 04:03 AM (20 days, 3 hours ago)

Updated post with plate progress.

Any advice around when I should think about starting T1 transfers?


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Re: LAGM v. 2.024 [Re: Womble]
    #28613624 - 01/08/24 04:43 AM (20 days, 2 hours ago)

Updated OP

Put spores to agar, first time using an inoculation loop so I feel like I fucked it up, also was too busy to pour new plates so I used an old PDA plate from a batch I was pretty displeased with but I couldnt care less and probably neither could the spores so here we go babyyy


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Re: LAGM v. 2.024 [Re: Tri-Polar]
    #28613834 - 01/08/24 08:47 AM (19 days, 22 hours ago)

I don’t use a loop anymore. Sterile swabs all day ❤️


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Re: LAGM v. 2.024 [Re: SupaThaRipper]
    #28613857 - 01/08/24 09:05 AM (19 days, 22 hours ago)

Updated OP with new pics


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Offlineedixo
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Re: LAGM v. 2.024 [Re: edixo] * 1
    #28613877 - 01/08/24 09:26 AM (19 days, 22 hours ago)

Quote:

edixo said:
08/01/2024

Liftoff! We have germination on plate 1! Now we wait for more.






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~ LAGM 2024 ~


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: Womble]
    #28614060 - 01/08/24 12:15 PM (19 days, 19 hours ago)

Quote:

Womble said:
Updated post with plate progress.

Any advice around when I should think about starting T1 transfers?





It's hard to say. I usually recommend to take a transfer as soon as you're certain it's mushroom mycelium and are able to make the transfer without any risk of grabbing spores in the process. But, on the other hand I do sometimes like to let it grow out and better establish itself incase it wants to present different growth habits.

It's hard to see what's going on in your plate so it's hard for me to give you better advice :shrug: Whenever you choose to take transfers though make sure you take several of each. Frequently I will put 2 or 3 transfer pieces to the same plat and make 2 plates. You have to work a little faster doing this method as the plates will become overgrown quickly, but it gives you a wide variety or growth to select from and it increases you chances of being able to select clean mycelium.

Good work so far, keep it up!


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OfflineWomble
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28614077 - 01/08/24 12:48 PM (19 days, 18 hours ago)

Quote:

bigfootscreepyuncl said:
Quote:

Womble said:
Updated post with plate progress.

Any advice around when I should think about starting T1 transfers?





It's hard to say. I usually recommend to take a transfer as soon as you're certain it's mushroom mycelium and are able to make the transfer without any risk of grabbing spores in the process. But, on the other hand I do sometimes like to let it grow out and better establish itself incase it wants to present different growth habits.

It's hard to see what's going on in your plate so it's hard for me to give you better advice :shrug: Whenever you choose to take transfers though make sure you take several of each. Frequently I will put 2 or 3 transfer pieces to the same plat and make 2 plates. You have to work a little faster doing this method as the plates will become overgrown quickly, but it gives you a wide variety or growth to select from and it increases you chances of being able to select clean mycelium.

Good work so far, keep it up!




I appreciate that the pics are really not that helpful, I really struggled with getting clear photographs. I'll have to have a play around with that.

Thanks for the pointers, I feel quite confident in what looks good and what doesn't and I know it takes time and experience, as with everything else in this hobby, so I don't feel like I'm completely fumbling about in the dark but it's good to get a feel for what works for everyone else and take it from there.

I'll pour more plates and take transfers tomorrow I see how that goes.


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Re: LAGM v. 2.024 [Re: Womble] * 1
    #28614096 - 01/08/24 01:10 PM (19 days, 18 hours ago)

very minor update to report the appearance of satellite growth on my Wild Coast plate which I actually don't think is contamination though! Also I believe I have germination on NecD finally


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Re: LAGM v. 2.024 [Re: SupaThaRipper]
    #28614428 - 01/08/24 07:09 PM (19 days, 12 hours ago)

Quote:

SupaThaRipper said:
I don’t use a loop anymore. Sterile swabs all day ❤️




Yeah thats gonna be my go to from now on, was kicking myself for not using one like halfway through the process haha


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Re: LAGM v. 2.024 [Re: edixo] * 1
    #28614747 - 01/09/24 02:56 AM (19 days, 4 hours ago)

Quote:

edixo said:
09/01/2024

Really nice growth on plate 1 already, so did a small transfer to some homemade agar to see if it work. If it does, I'll try to put it to grain.






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~ LAGM 2024 ~


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Re: LAGM v. 2.024 [Re: Tri-Polar]
    #28614810 - 01/09/24 05:17 AM (19 days, 2 hours ago)

Quote:

Tri-Polar said:
Quote:

SupaThaRipper said:
I don’t use a loop anymore. Sterile swabs all day ❤️




Yeah thats gonna be my go to from now on, was kicking myself for not using one like halfway through the process haha





Swabs:

I've never used one

What does everyone like about them?

When you're using a swab, what's your preferred source of spores, meaning swiping a fresh gill, swiping a print, or shooting spore solution onto the tip?


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Re: LAGM v. 2.024 [Re: mnj] * 5
    #28614821 - 01/09/24 05:31 AM (19 days, 2 hours ago)

Quote:

mnj said:

Swabs:

I've never used one

What does everyone like about them?

When you're using a swab, what's your preferred source of spores, meaning swiping a fresh gill, swiping a print, or shooting spore solution onto the tip?




I hate swabs, and wouldn’t use them if I didn’t have to. I only use them to grab spores from variants that won’t drop them.

I’ve tried using them instead of a loop to transfer from a print to agar. They work, but hold a lot of spores in the tip. I end up having to break it off and shove it in. A loop works well, is easy to flame sterilize, and is not yet another disposable thing to get tossed in the landfill. I don’t know why you would ever have to shoot spore solution onto a swab when you can just drop it onto a plate.


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OfflineSilentraindrops
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Re: LAGM v. 2.024 [Re: RoscoeReturns] * 1
    #28614841 - 01/09/24 06:01 AM (19 days, 1 hour ago)

Quote:

RoscoeReturns said:
Quote:

mnj said:

Swabs:

I've never used one

What does everyone like about them?

When you're using a swab, what's your preferred source of spores, meaning swiping a fresh gill, swiping a print, or shooting spore solution onto the tip?




I hate swabs, and wouldn’t use them if I didn’t have to. I only use them to grab spores from variants that won’t drop them.

I’ve tried using them instead of a loop to transfer from a print to agar. They work, but hold a lot of spores in the tip. I end up having to break it off and shove it in. A loop works well, is easy to flame sterilize, and is not yet another disposable thing to get tossed in the landfill. I don’t know why you would ever have to shoot spore solution onto a swab when you can just drop it onto a plate.



never used a swab everything you said is about why I don't go through the effort of a clean swab...
I like loops and MSS. I can see why you'd use them for gathering the spores tho i will try that one day soon :smile:.

Squeezing the side of a syringe for a drop is so easy .... I even like turning my print into a syringe :smile:. Idk if my prints are very clean but its fun lol. My b+ print was surprisingly clean I didn't get a ton of contams when i was testing all sorts of methods. idk.
i did loop , sprinkle/tap  , mss, hell i let the water run off the foil into the agar lol.... I didn't use a q tip... that's what would of done it :P.


--------------------


Edited by Silentraindrops (01/09/24 11:14 AM)


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Re: LAGM v. 2.024 [Re: Silentraindrops] * 1
    #28614891 - 01/09/24 07:09 AM (19 days, 31 minutes ago)

Quote:

Silentraindrops said:Idk if my prints are sterile but its fun lol




Prints, no matter how clean they are, will not ever be sterile. For one, if it were truly sterile your spores would no longer be viable. Plus, mushrooms are grown in open air and handled a significant amount in order to harvest the spores. Even if the fruits were grown in a positive pressure clean-room it is still highly likely that there would be some bacteria and/or mold present.

In this hobby there is a huge difference between clean and sterile and it's critical to know the difference.


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Re: LAGM v. 2.024 [Re: mnj]
    #28614972 - 01/09/24 08:41 AM (18 days, 22 hours ago)

I’ll swab gills or a print and both work extremly well. I still have my loop. Need to throw that thing in the trash 😂 useless pos lol. If I use a mss, i just squirt directly to agar. Loops are shit for grabbing spores compared to swabs.

To test my statement, take a spore print that you think has been wiped completely clear of all spores. Now use a swab on it, bet you’ll fill that swab right up.

@roscoe, yeah I definitely break my swabs off and leave them in the dish. You’re definitely right about that. They do hang on. I’ll usually swipe a dish, and then on a second dish, I’ll break the swab off. They both usually germinate though. Sometimes at the same time. Sometimes one before the other or vice versa


Edited by SupaThaRipper (01/09/24 08:47 AM)


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Re: LAGM v. 2.024 [Re: SupaThaRipper]
    #28614982 - 01/09/24 08:53 AM (18 days, 22 hours ago)

Thanks for the replies y'all.

Silenraindrops, I never thought of squeezing the sides of a syringe to get a small drop out, might have to give that a go. But would the syringe suck in ambient air to replace the loss of mss when I release my squeeze? I imagine that's why the plunger is used.

5 out of 8 plates I streaked with the loop have not germinated, but also haven't shown any contam, so I may open them back up and shoot a drop off mss in there.


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InvisibleReverendMyc

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Re: LAGM v. 2.024 [Re: ReverendMyc] * 2
    #28614985 - 01/09/24 08:53 AM (18 days, 22 hours ago)

Quote:

RoscoeReturns said:
I hate swabs, and wouldn’t use them if I didn’t have to. I only use them to grab spores from variants that won’t drop them.

I’ve tried using them instead of a loop to transfer from a print to agar. They work, but hold a lot of spores in the tip. I end up having to break it off and shove it in. A loop works well, is easy to flame sterilize, and is not yet another disposable thing to get tossed in the landfill. I don’t know why you would ever have to shoot spore solution onto a swab when you can just drop it onto a plate.



:whathesaid: x 100.

I use them to get spores from non-dropping varieties but then usually resort to making brf pucks to germinate them. Then transfer to agar to clean, so just extra steps. Plus, you basically get one or at most two uses out of a swab while a loop on a print can be used many times. They work from prints, but seem wasteful to me since most of the spores end up staying on the swab.
EDIT: plus more of a pita to ship than prints. Love to share, hate shipping and handling and special non-bendable postage and envelopes required with swabs.

Speaking of which, that is what I did last night with the remainder of a couple of swabs that also went to agar for LAGM. The remaining spores on the swabs got dipped in sterile water and shoved into bfr pucks. As well as a few transfers from two of the more aggressive LAGM plates.

Quote:

ReverendMyc said:
2024-01-08
Transferred the most aggressive two plates to some new plates.
Mexicana Chicon Nindo from print & Pan Cyan Bunnell from print
Still waiting on the swabs to get started.


Also did some other things that I started before the 1st.





OP updated.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




Edited by ReverendMyc (01/09/24 09:01 AM)


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Re: LAGM v. 2.024 [Re: ReverendMyc] * 2
    #28614999 - 01/09/24 09:08 AM (18 days, 22 hours ago)

I’ll usually hand out swabs in my giveaways. Can make dozens off one print vs taking dozens of prints. Saves a lot of time. I also started getting 3 or 4” swabs. Which have been fine st the post office for sending as regular, flexible mail. For the longer swabs though, you’re right. You need to pay $1 for the non flex mail, otherwise they’ll send them through the rollers and break the sticks. You can also swab to swab to get more spores as well. I’ve done hundreds of swabs and have only ever had one set not germinate. Same thing can happen with prints though. I’ve had prints not germinate as well. It’s also why I recommend breaking the swabs off in the dish and smashing them in the agar. This is the way to go. Swipe one plate, smash another.

Here’s a picture of an aggressive swab I had just for shits and giggles on 1% lol




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Re: LAGM v. 2.024 [Re: SupaThaRipper] * 1
    #28615029 - 01/09/24 09:31 AM (18 days, 22 hours ago)

Ya. Just banter. I know that swabs work fine, I just prefer prints and loops. I have success and failures with both depending a lot on age and species. I will have to look for the short flexible swabs in the future for PE varieties.

This is a Jack Frost from swab plate that I took transfers from last night.

If I do swabs (not from prints), I usually wet them in water that I sterilized in cryo tubes for the purpose. Then tear off little pieces and shove them into the agar, or a brf puck if they are stubborn. Works all the time most of the time.

Just sharing why I prefer one method over the other for those who don't have our level of experience that will read our witty repartee.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




Edited by ReverendMyc (01/09/24 09:33 AM)


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Re: LAGM v. 2.024 [Re: ReverendMyc] * 1
    #28615038 - 01/09/24 09:37 AM (18 days, 22 hours ago)

Oh dude I’m strictly here just to argue 🤣 no I know man it all comes down to preference and we all have our own methods for sure. Prints are definitely better when it comes to always having spores to go back to as well!

So water on the swab is the trick for pulling off fibers huh. I’ve tried doing it dry before and couldn’t understand how people make it work lol.


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Re: LAGM v. 2.024 [Re: SupaThaRipper]
    #28615042 - 01/09/24 09:40 AM (18 days, 22 hours ago)

Sad update :tongue:


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28615044 - 01/09/24 09:42 AM (18 days, 21 hours ago)

Dude don’t give up on those plates! Transfer to brf pucks!


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Re: LAGM v. 2.024 [Re: SupaThaRipper] * 1
    #28615047 - 01/09/24 09:45 AM (18 days, 21 hours ago)

Quote:

SupaThaRipper said:
Dude don’t give up on those plates! Transfer to brf pucks!



I actually don't NEED them anyway. I had some better looking ones from another MSS syringe I'd started earlier in December, so they don't qualify for LAGM, but I put it to grain last night


J-014 thru J-017 taken 01/08/24


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28615049 - 01/09/24 09:46 AM (18 days, 21 hours ago)

Ahhh well if they’re not a need, then fuck em 🤣


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OfflineSilentraindrops
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28615116 - 01/09/24 11:10 AM (18 days, 20 hours ago)

Quote:

bigfootscreepyuncl said:
Quote:

Silentraindrops said:Idk if my prints are sterile but its fun lol




In this hobby there is a huge difference between clean and sterile and it's critical to know the difference.




I know  I know , I'll change to very clean. Also the ones i grab for prints are grown in a "sealed chamber", I 100% know that I'm only lowering the odds but it's nice to think I'm giving them a good chance.
I've also been goofing off with some open air experiments with my agar. When I go looking for contams i cant get them, But when I .... bam.


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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28615118 - 01/09/24 11:12 AM (18 days, 20 hours ago)

You could have cleaned those up in a number of transfers using a few little tricks, but yeah, your other plates are much better. Good call :rockon: it's easy enough for work to pile up in this hobby so if you don't need to save a culture, then fuck it :lol:


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28615129 - 01/09/24 11:22 AM (18 days, 20 hours ago)

Quote:

bigfootscreepyuncl said:
You could have cleaned those up in a number of transfers using a few little tricks, but yeah, your other plates are much better. Good call :rockon: it's easy enough for work to pile up in this hobby so if you don't need to save a culture, then fuck it :lol:



Yeah I definitely have a good amount of irons in the fire

  • AA+ tub goin (albeit badly)
  • KSSS tub goin (albeit badly)
  • inoculated 4 grain jars with JMF last night
  • inoculated 4 grain jars with Mazatapec last night
  • have 2 plates of Enigma rockin
  • have several spore samples in the mail, of which I'll be selecting two for LAGM


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Re: LAGM v. 2.024 [Re: mycosispeon] * 3
    #28615177 - 01/09/24 12:08 PM (18 days, 19 hours ago)

An update. First Transfers!



--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28615289 - 01/09/24 01:39 PM (18 days, 18 hours ago)

Op updated don’t know how to link it. Just some germ pics

Nvm, thanks captain!
OP up hither


Edited by EniQma (01/09/24 01:57 PM)


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Re: LAGM v. 2.024 [Re: EniQma]
    #28615298 - 01/09/24 01:51 PM (18 days, 17 hours ago)

Quote:

EniQma said:
Op updated don’t know how to link it. Just some germ pics




Copy the link (right click on PC or Hold on Phone) the blue #xxxxxxxxx number on your OP. Choose copy link address.

You then press the globe with an infinity symbol at the top of the text box for your new post. It will open a window where you can paste the link you copied.


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Re: LAGM v. 2.024 [Re: CaptainPuffy] * 1
    #28616234 - 01/10/24 10:40 AM (17 days, 21 hours ago)



--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Re: LAGM v. 2.024 [Re: mycosispeon] * 2
    #28616246 - 01/10/24 10:51 AM (17 days, 20 hours ago)

Finally nocced up my plates! Better late then never!

Update


--------------------
Vibetyme's LAGM 2.022

Vibetyme's LAGM_23

Vibetyme's LAGM_24

                 


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Re: LAGM v. 2.024 [Re: Vibetyme] * 3
    #28616274 - 01/10/24 11:21 AM (17 days, 20 hours ago)

Minor update with no pics, just updated to say that we've got confirmed germination on my Jambos and now every one of my varieties has sprung to life :rockon:

Mycelium is growing from a clump of spores but there is also a very small colony of bacteria growing directly on my streak line so this may be a difficult culture to clean. Time will tell and I'll give 'er hell!


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28616947 - 01/10/24 09:32 PM (17 days, 10 hours ago)

Updated OP

Put more spores to agar this time with a swab, really hit me in that moment- you can make metric fuck tons of swabs from a single print if you're so inclined, neat.


--------------------
Intro to Shroomery:raver2:The Sexy TEKs

IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH.

hey fuck you dont look at me


Edited by Tri-Polar (01/10/24 09:33 PM)


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OfflineSeventhMushroom
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Re: LAGM v. 2.024 [Re: Tri-Polar] * 1
    #28617052 - 01/11/24 12:38 AM (17 days, 7 hours ago)

OP updated. I have life! My plates are a bit behind the curve, but 3/6 have some sort of germination today!


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Re: LAGM v. 2.024 [Re: SeventhMushroom]
    #28617155 - 01/11/24 04:08 AM (17 days, 3 hours ago)

11/1/24
Signs of germination from both strains (Shakti and YUxGM) not fluffy white mycelium, but visible differences on top face of agar.


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Re: LAGM v. 2.024 [Re: r0kovski]
    #28617174 - 01/11/24 04:33 AM (17 days, 3 hours ago)

Updated me log

The slow growth is agonizing :boo:


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Offlineedixo
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Re: LAGM v. 2.024 [Re: edixo] * 1
    #28618262 - 01/12/24 02:49 AM (16 days, 4 hours ago)

Quote:

edixo said:
12/01/2024

Plate 1 has some very even, solid growth going on, so did 3 transfers of that to some store-bought PCA. The homemade agar -seems- to be working, but it's slow.






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Re: LAGM v. 2.024 [Re: mycosispeon] * 1
    #28618647 - 01/12/24 12:01 PM (15 days, 19 hours ago)

OP updated

Quote:

mycosispeon said:
01/12/24

Observations

So far, nothing on the dishes I inoculated with TAT. :frown: Fortunately, I've got germination on one of the dishes of Ghost. :smile:





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LAGM 2.024

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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28618673 - 01/12/24 12:43 PM (15 days, 18 hours ago)

Quote:

mycosispeon said:
OP updated

Quote:

mycosispeon said:
01/12/24

Observations

So far, nothing on the dishes I inoculated with TAT. :frown: Fortunately, I've got germination on one of the dishes of Ghost. :smile:









Fuck yeah, be patient. I'm surprised you've got something so soon :rockon:


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28618675 - 01/12/24 12:45 PM (15 days, 18 hours ago)

Quote:

bigfootscreepyuncl said:
Quote:

mycosispeon said:
OP updated

Quote:

mycosispeon said:
01/12/24

Observations

So far, nothing on the dishes I inoculated with TAT. :frown: Fortunately, I've got germination on one of the dishes of Ghost. :smile:









Fuck yeah, be patient. I'm surprised you've got something so soon :rockon:



After my hack job of inoculating with swabs, I was a bit concerned


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Re: LAGM v. 2.024 [Re: mycosispeon] * 1
    #28618691 - 01/12/24 01:03 PM (15 days, 18 hours ago)

Time will tell. There's nothing you can do now anyway except sit back and observe. Even if it is mold watch it and see how it behaves so you can spot it again in the future.


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InvisibleReverendMyc

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Re: LAGM v. 2.024 [Re: ReverendMyc] * 1
    #28618719 - 01/12/24 01:42 PM (15 days, 17 hours ago)

Minor updates

Quote:

ReverendMyc said:
2024-01-10
Definitely seeing germ on Mex Chicon Nindo and Subtrop Hindsight. Hints of germ on the final two as well.

2024-01-12
Out of town for a while and missing them already. They grow up so fast.




--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
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Re: LAGM v. 2.024 [Re: ReverendMyc]
    #28619135 - 01/12/24 08:07 PM (15 days, 11 hours ago)



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Re: LAGM v. 2.024 [Re: ReverendMyc] * 1
    #28619218 - 01/12/24 09:26 PM (15 days, 10 hours ago)

T1 done Update


--------------------
Make my Funk the P Funk, I wants to get Funked up

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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: Gastronomicus]
    #28619949 - 01/13/24 03:43 PM (14 days, 15 hours ago)

We've got action! finally took T1's. Depending on how these grow out I'll probably send T2 to grain/LC.

I'll need to pour another sleeve or 5 pretty soon and after looking at some of the growth I have on my plates compared to other growth I've seen while studying this species I may change my agar recipe, or pour some pan specific plates just cuz I'm fancy


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28620127 - 01/13/24 07:08 PM (14 days, 12 hours ago)

Quote:

bigfootscreepyuncl said:
We've got action! finally took T1's. Depending on how these grow out I'll probably send T2 to grain/LC.

I'll need to pour another sleeve or 5 pretty soon and after looking at some of the growth I have on my plates compared to other growth I've seen while studying this species I may change my agar recipe, or pour some pan specific plates just cuz I'm fancy



Nice job! I think that'd make you first to grain. Do you use the metal straw trick for your transfers?


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28620150 - 01/13/24 07:28 PM (14 days, 12 hours ago)

OP updated with some nasty contam agar pics.


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28620164 - 01/13/24 07:47 PM (14 days, 11 hours ago)

Quote:

mycosispeon said:
Quote:

bigfootscreepyuncl said:
We've got action! finally took T1's. Depending on how these grow out I'll probably send T2 to grain/LC.

I'll need to pour another sleeve or 5 pretty soon and after looking at some of the growth I have on my plates compared to other growth I've seen while studying this species I may change my agar recipe, or pour some pan specific plates just cuz I'm fancy



Nice job! I think that'd make you first to grain. Do you use the metal straw trick for your transfers?





I do. It seems you either love it or hate it. For me personally I'm faster and cleaner than I am with a scalpel, the perfect circles are just an added bonus if you'd even want to call it that.

sometimes the transfer piece gets stuck in the straw which is always a pain in the ass and it seems to always happen on the best piece you're trying to transfer lol, but in my opinion it's worth the small amount of headache.


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28620505 - 01/14/24 03:45 AM (14 days, 3 hours ago)

Metal straw technique you say...might have to dig out my childhood pea shooter and get to work

Updated me log

January 14, 2024

Woke up nice and early so I cleaned off the kitchen counter and brought out the SAB.

Shot some of the pasty plates I made last weekend with mss of Pan Cambo Thai and AA+, two plates of each.

Made T1 transfers of Penis Envy and AA+.  I wasn't impressed with either variety's germ plate, but thought I'd make the transfers and see what happens.

No pics of these plates because the condensation is ridiculous.

My Pan Cyan Hawaii germ plate is coming along with several germination points.  One has spread far enough for transfers but the other two are not yet ready so I'm gonna let it ride a little longer:



Gonna cook more plates today so I'll be ready for transfers whenever the donor plates are ready


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


Edited by mnj (01/14/24 09:24 AM)


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InvisibleMojaveMyc
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Re: LAGM v. 2.024 [Re: mnj]
    #28620862 - 01/14/24 11:18 AM (13 days, 20 hours ago)

OP Updated. T1s are taking off. I'm pretty happy with how they look, will probably put a few of the pans to grain in the next day or two.


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: MojaveMyc]
    #28620987 - 01/14/24 12:47 PM (13 days, 18 hours ago)

Small update to include pictures ~14 hours after T1...I can not believe how fast these suckers are :rockon:


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28621132 - 01/14/24 02:30 PM (13 days, 17 hours ago)

Nice plates MojaveMyc

And holy shit creepyuncl those are moving fast


--------------------
LAGM 2024

:sagetrip:

Thank yoU
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28621186 - 01/14/24 03:24 PM (13 days, 16 hours ago)

Quote:

bigfootscreepyuncl said:
Small update to include pictures ~14 hours after T1...I can not believe how fast these suckers are :rockon:



Does that mean you are going agar => LC => grain?


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28621241 - 01/14/24 04:20 PM (13 days, 15 hours ago)

Quote:

mycosispeon said:
Quote:

bigfootscreepyuncl said:
Small update to include pictures ~14 hours after T1...I can not believe how fast these suckers are :rockon:



Does that mean you are going agar => LC => grain?





That's the tentative plan, but yes. Inoculate an LC broth with a tiny piece of agar (same as if I were transferring it to another agar plate), allow it to colonize then go to grain.

I've had shit success in the past with LC though so I'm debating whether I'll do any LC or just go agar to grain like I usually do.

In my opinion LC is much riskier as far as contaminants go but the payoff is jars/bags of grain finish colonizing in a fraction of the time. I'm rarely in a hurry for my grains to colonize though so I haven't spent much time perfecting that practice


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Re: LAGM v. 2.024 [Re: Noncense] * 1
    #28621433 - 01/14/24 07:41 PM (13 days, 11 hours ago)

Minor Update

Germination!


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: thirdeyewild]
    #28621445 - 01/14/24 07:50 PM (13 days, 11 hours ago)

Quote:

thirdeyewild said:
Minor Update

Germination!





Hell yeah, got some live action :rockon: You've got me curious though...what's that Brainfreeze you mentioned in your log?


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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28621478 - 01/14/24 08:25 PM (13 days, 11 hours ago)

Brainfreeze is a Tidalwave-jack frost cross I made. Apurplecrayon had a beautiful go at it.

Pictures

The tidalwave culture I used often makes mutations that look like little brains.


--------------------


Edited by thirdeyewild (01/14/24 08:28 PM)


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: thirdeyewild] * 1
    #28621492 - 01/14/24 08:33 PM (13 days, 11 hours ago)

Oooo that is a very pretty fruit! I love the paddy hat caps on it :smile:


I have so many varieties I want to run but so little time...especially if I want to do it properly and run several generations finding a good culture instead of just doing a one time run from spore...I think that's how everyone is though :lol:


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Offlineedixo
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Re: LAGM v. 2.024 [Re: edixo]
    #28621717 - 01/15/24 03:56 AM (13 days, 3 hours ago)

Quote:

edixo said:
15/01

Plate 1 is so far the only one with growth, but it's going so strong, and so are the transfers. I decided to put some to grain directly from the plate instead of waiting for the transfers to grow out.






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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: edixo]
    #28621824 - 01/15/24 07:38 AM (13 days, 2 minutes ago)

Quote:

edixo said:
Quote:

edixo said:
15/01

Plate 1 is so far the only one with growth, but it's going so strong, and so are the transfers. I decided to put some to grain directly from the plate instead of waiting for the transfers to grow out.










Daaaang you put that tiny little transfer wedge to grains?? Nice


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Offlineedixo
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28621909 - 01/15/24 08:59 AM (12 days, 22 hours ago)

Quote:

bigfootscreepyuncl said:
Quote:

edixo said:
Quote:

edixo said:
15/01

Plate 1 is so far the only one with growth, but it's going so strong, and so are the transfers. I decided to put some to grain directly from the plate instead of waiting for the transfers to grow out.










Daaaang you put that tiny little transfer wedge to grains?? Nice



Nah, that tiny wedge is currently a fuzzball on some homemade agar.


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Re: LAGM v. 2.024 [Re: edixo]
    #28622059 - 01/15/24 11:25 AM (12 days, 20 hours ago)

Updated Op with new pics of plates.


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Re: LAGM v. 2.024 [Re: RockinRobot]
    #28622068 - 01/15/24 11:32 AM (12 days, 20 hours ago)

OP updated with pix

Quote:

mycosispeon said:
01/15/24

Observations

Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.




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LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28622080 - 01/15/24 11:46 AM (12 days, 19 hours ago)

Quote:

mycosispeon said:
OP updated with pix

Quote:

mycosispeon said:
01/15/24

Observations

Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.








Very nice! Looks like you'll definitely have enough myc to work with but you'll likely need to do a couple transfers. I'm sure you're aware but you've got some major bacteria colonies on some of your plates (bacteria in isolated colonies is usually a non-issue at all to work around).

The spot(s) that have me the most concerned are on your Ghost swab plate are circled in blue, particularly the patch of mycelium forming below/behind the swab stick. I would be looking to transfer from the areas circled in red, and avoid/keep an eye on the areas circled in blue:



If you have the option, meaning, if the fuzz underneath the stick doesn't start changing colors I would wait a little long to let the red areas grow out a little further to before transferring. But, if you have to transfer sooner to avoid contaminants then that's the name of the game.

Looking pretty good :rockon:


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Offlinemycosispeon
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28622086 - 01/15/24 11:52 AM (12 days, 19 hours ago)

Quote:

bigfootscreepyuncl said:
Quote:

mycosispeon said:
OP updated with pix

Quote:

mycosispeon said:
01/15/24

Observations

Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.








Very nice! Looks like you'll definitely have enough myc to work with but you'll likely need to do a couple transfers. I'm sure you're aware but you've got some major bacteria colonies on some of your plates (bacteria in isolated colonies is usually a non-issue at all to work around).

The spot(s) that have me the most concerned are on your Ghost swab plate are circled in blue, particularly the patch of mycelium forming below/behind the swab stick. I would be looking to transfer from the areas circled in red, and avoid/keep an eye on the areas circled in blue:



If you have the option, meaning, if the fuzz underneath the stick doesn't start changing colors I would wait a little long to let the red areas grow out a little further to before transferring. But, if you have to transfer sooner to avoid contaminants then that's the name of the game.

Looking pretty good :rockon:



Yep I keyed in on those red areas too. I'll keep an eye on it. What do you think about the other 3 dishes? It looks like both of the Ghost dishes have some of that "wet" mold. The two TAT dishes don't have any of that, but whatever is growing looks a little fuzzy, like cottony.


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



Edited by mycosispeon (01/15/24 11:53 AM)


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28622145 - 01/15/24 12:49 PM (12 days, 18 hours ago)

Quote:

mycosispeon said:
Quote:

bigfootscreepyuncl said:
Quote:

mycosispeon said:
OP updated with pix

Quote:

mycosispeon said:
01/15/24

Observations

Well, I've got something... Germination on all four dishes now. The TAT is a little on the fuzzy size. The Ghost is too, but there's one sector in the second dish of that, that looks stronger.








Very nice! Looks like you'll definitely have enough myc to work with but you'll likely need to do a couple transfers. I'm sure you're aware but you've got some major bacteria colonies on some of your plates (bacteria in isolated colonies is usually a non-issue at all to work around).

The spot(s) that have me the most concerned are on your Ghost swab plate are circled in blue, particularly the patch of mycelium forming below/behind the swab stick. I would be looking to transfer from the areas circled in red, and avoid/keep an eye on the areas circled in blue:



If you have the option, meaning, if the fuzz underneath the stick doesn't start changing colors I would wait a little long to let the red areas grow out a little further to before transferring. But, if you have to transfer sooner to avoid contaminants then that's the name of the game.

Looking pretty good :rockon:



Yep I keyed in on those red areas too. I'll keep an eye on it. What do you think about the other 3 dishes? It looks like both of the Ghost dishes have some of that "wet" mold. The two TAT dishes don't have any of that, but whatever is growing looks a little fuzzy, like cottony.







The area in blue is a large, isolated bacteria colony. Is that what you were referring to as the wet mold? Those are easy to work around. Basically just don't touch them. Bacteria doesn't move very fast or sporulate so as long as it's away from your culture so is generally nothing to worry about. Bacteria within your culture is another story.



That blue circle is gonna be real nice in a few days. Be patient and transfer from there (assuming nothing weird happens between now and then).



I would transfer from any of these red areas. It all looks pretty clean for a germ plate. But, if it comes down to it I'd definitely use the mycelium from the swab plate. And why the fuck did you stab the plate so many times? :lol:

I'm just bustin your balls. Swabs are a MF to deal with if you're not used to them. That's why if I have 2 I'll just jam the first one in and if it doesn't work then on the second swab I'll tease out fibers and be a little more delicate.

Everything looks good amigo keep us posted!


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OfflineCaptainPuffy
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28622165 - 01/15/24 01:07 PM (12 days, 18 hours ago)

OP updated.

Dealing with bacteria on my Pan Cyan plates. Attempting to clean it up. Plan to do 1 transfer and then transfer again to water agar asap. I'm leaving for work tomorrow, so it will have to wait a week.


Edited by CaptainPuffy (01/15/24 01:08 PM)


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Offlinemycosispeon
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28622170 - 01/15/24 01:10 PM (12 days, 18 hours ago)

Quote:

bigfootscreepyuncl said:


I would transfer from any of these red areas. It all looks pretty clean for a germ plate. But, if it comes down to it I'd definitely use the mycelium from the swab plate. And why the fuck did you stab the plate so many times? :lol:

I'm just bustin your balls. Swabs are a MF to deal with if you're not used to them. That's why if I have 2 I'll just jam the first one in and if it doesn't work then on the second swab I'll tease out fibers and be a little more delicate.

Everything looks good amigo keep us posted!



LOL yeah yeah yeah. I hear ya. Hack job, like I said. I couldn't for the life of me get the gd fibers to come off of my tweezers!

It's all good. I'll see how they look tomorrow or Wed


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- I'm new so take everything I say with a grain of salt :wink:



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InvisibleLyleChipperson


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Posts: 61
Re: LAGM v. 2.024 [Re: mycosispeon] * 1
    #28623554 - 01/16/24 02:02 PM (11 days, 17 hours ago)

Can I join in? I got new spores last week for my second grow ever so I put them to agar.

1/11

Inoculated 6 plates each with Burmese PE spore print and Natalensis swab. Agar recipe was PDA from this forum. 5 days in and there is no growth yet.

1/18
There is visible growth on the Natalensis swab plate, all the others have only developed small contaminate blotches so far.



1/19

The BPE is still showing no signs of growth, so today I'll get more plates started with Pan Cyans and PF Redspore. I wasted 2 months trying to clean up dirty GT cultures unsuccessfully, so this time I'll get everything I have on agar and I'll pick the cleanest growth in the end. Hopefully this carpet bombing approach will give better results.


1/25

Took 10 transfers from the Natalensis swab plate, all the other plates still have nothing going on. I couldn't see the tiny contaminate well in the SAB so I may have transferred it along to some plates, but hopefully I will get enough clean ones out of the 10 to keep it going. This time I tried oven sterilizing my plates for 90 mins at 200 c and my agar for 1 hour in the PC to see if it will make a difference, since I didn't have that contaminate before, but it was present in my last 2 sessions and there have been no changes in the way I do it.


Edited by LyleChipperson (01/26/24 07:32 AM)


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OfflineGastronomicus
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Re: LAGM v. 2.024 [Re: LyleChipperson]
    #28623610 - 01/16/24 03:01 PM (11 days, 16 hours ago)

Of course! Welcome


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Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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OfflineNikoyo
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Re: LAGM v. 2.024 [Re: Gastronomicus] * 1
    #28623618 - 01/16/24 03:10 PM (11 days, 16 hours ago)

Small update on the op with some pics. Taking t2s on some of the cultures tonight; my mvp plates are far ahead of the rest :willynilly:


--------------------
Gettin back to work


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OfflineAndImTheHighOne
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Re: LAGM v. 2.024 [Re: Nikoyo]
    #28623671 - 01/16/24 03:55 PM (11 days, 15 hours ago)

I have typed out a nearly finished update twice today only to forget about it and have it deleted by a page refresh 😭 From now on I'm starting every update in my notes and only adding pictures at the end. Bear with me guys, I will re-type it once again and get an update out today.


--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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OfflineAndImTheHighOne
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Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28623925 - 01/16/24 07:39 PM (11 days, 12 hours ago)

Updated my OP.  Finally.

Quote:

AndImTheHighOne said:
**UPDATE 1/16/24** (!) All T1s Complete (!), First Plates Contam, Bad Ecuador, and My Plan Moving Forward

Alright, alright, alright we've got a lot to cover as this is my first update in A WEEK! For anyone who is following along, I certainly don't want to make you wait too long between updates and lose your interest. But what is LAGM without a bit of suspense, right?!

So without further ado, I present my eight T1 transfers:



Organized by row, from top to bottom, we have: Tosohatchee, Hanoi, P. natalensis, and Golden Koh F2

Continued...





--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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OfflineSeventhMushroom
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Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28624151 - 01/17/24 01:19 AM (11 days, 6 hours ago)

OP updated. Making a risky bet on some LC :P

I'm being very lazy here. I don't feel like making more agar plates, but happen to have some ready-to-go LC vials sitting around. So, "transfer" to LC, everything should be fine. nothing could possibly go wrong. And if it does, I mean, I guess that just means I need to do the thing I wasn't doing anyways. No real loss but time.



--------------------
LAGM 2024


Edited by SeventhMushroom (01/17/24 01:20 AM)


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OfflineTri-Polar
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Re: LAGM v. 2.024 [Re: SeventhMushroom]
    #28624628 - 01/17/24 01:49 PM (10 days, 17 hours ago)

Updated OP


good news we got germination, bad news im sick af with covid

Win some lose some


--------------------
Intro to Shroomery:raver2:The Sexy TEKs

IF I HAVE SEEN FURTHER IT IS BY STANDING UPON THE SHOULDERS OF GIANTS, BITCH.

hey fuck you dont look at me


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Offlinemycosispeon
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Re: LAGM v. 2.024 [Re: Tri-Polar] * 1
    #28624669 - 01/17/24 02:44 PM (10 days, 16 hours ago)

OP Updated...

The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Onlinefiddle_headS
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Re: LAGM v. 2.024 [Re: Noncense] * 2
    #28624718 - 01/17/24 03:29 PM (10 days, 16 hours ago)

Everyone is looking great so far. I´m excited for my second lagm to begin i just have to finish my current projects. alot of my germ plates will be f1 and f2 generations of my current grows. there are some i am germing like kodama which i have not grown previously. best of luck to everyone and cant wait to join in.


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28625161 - 01/17/24 07:35 PM (10 days, 12 hours ago)

Quote:

mycosispeon said:
OP Updated...

The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.





Those satellite colonies on your ghost plate have me a little concerned though. Did the swab roll around on the plate at all or did you streak it on the surface? If not I'd be looking into transferring as soon as you have time. If those satellites are in fact mold, once they start sporulating it will get a lot harder to clean a culture.

Lookin' good though!


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Offlinemycosispeon
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28625446 - 01/18/24 12:14 AM (10 days, 7 hours ago)

Quote:

bigfootscreepyuncl said:
Quote:

mycosispeon said:
OP Updated...

The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.





Those satellite colonies on your ghost plate have me a little concerned though. Did the swab roll around on the plate at all or did you streak it on the surface? If not I'd be looking into transferring as soon as you have time. If those satellites are in fact mold, once they start sporulating it will get a lot harder to clean a culture.

Lookin' good though!



Shit... No, the swab didn't roll around, nor did I streak. I'm pouring new agar dishes right now. Hopefully, if it IS mold, it doesn't sporulate overnight.


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28625502 - 01/18/24 04:07 AM (10 days, 3 hours ago)

Quote:

mycosispeon said:
Quote:

bigfootscreepyuncl said:
Quote:

mycosispeon said:
OP Updated...

The two dishes with the swab in them seem to be shaping up to be my transfer candidates. I think I should wait another day though.





Those satellite colonies on your ghost plate have me a little concerned though. Did the swab roll around on the plate at all or did you streak it on the surface? If not I'd be looking into transferring as soon as you have time. If those satellites are in fact mold, once they start sporulating it will get a lot harder to clean a culture.

Lookin' good though!



Shit... No, the swab didn't roll around, nor did I streak. I'm pouring new agar dishes right now. Hopefully, if it IS mold, it doesn't sporulate overnight.






don't let me get you worked up, I've already cried mold once :lol: but this time I'm more certain...the neat thing about agar is you can't really screw up on an irreparable level. You have as many 'do overs' as you want up until you drop a wedge into the grain jar. Nothing to lose any sleep over, just transfer away from it as soon as you get a chance :thumbup:

I didn't even have time look at my plates yesterday. I'm sure they're due for a transfer which works out pretty nicely. I've got 5 varieties and 5 poured plates left. Now I just need to find a little time to make some cuts. That won't be today though.


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28625901 - 01/18/24 12:59 PM (9 days, 18 hours ago)

Major update without any pics - Bigfoots going back to spore because I'm pretty certain I have only been growing (and transferring) mold at this point in time lol. I'm waiting to hear back from some experts on the species but I know what I'm looking at...





This isn't exactly how I wanted to spend my day recovering from an adderall relapse bender but it seems like a justified and fitting punishment if I'm being honest. It's just one of those days!


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OnlineEniQma
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28625957 - 01/18/24 02:03 PM (9 days, 17 hours ago)

Quote:

bigfootscreepyuncl said:
Major update without any pics - Bigfoots going back to spore because I'm pretty certain I have only been growing (and transferring) mold at this point in time lol. I'm waiting to hear back from some experts on the species but I know what I'm looking at...





This isn't exactly how I wanted to spend my day recovering from an adderall relapse bender but it seems like a justified and fitting punishment if I'm being honest. It's just one of those days!




Haha bro I knew you were speedin your tits off with all tho posting you were doing! I was also wondering how the hell you lucked out with such fast colonizers.

Don’t beat yourself down. Shit happens


Edited by EniQma (01/18/24 02:03 PM)


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: EniQma]
    #28626045 - 01/18/24 03:15 PM (9 days, 16 hours ago)

Quote:

EniQma said:Haha bro I knew you were speedin your tits off with all tho posting you were doing! I was also wondering how the hell you lucked out with such fast colonizers.

Don’t beat yourself down. Shit happens





Well, several years ago JCM went from agar to harvest in like 12 days and I'm working with one of those prints so I just thought I got lucky with a lightning quick culture (and I was expecting this speed to be linear across all varieties, which is not the case). His thread is the one I studied the most, especially the culture selection section.

What I think actually happened is I have been using yeast plates lately for my cubes which provides a thicker, more robust growth habit for the mycelium, and the yeast caused the otherwise thin and wispy growth (desired for this species) to express itself in a much more tomentose form (not desired for this species) so I avoided transferring from the growth I should have been targeting. Meanwhile, mold mycelium didn't give a single fuck about the yeast in the agar and expressed itself as it naturally does, thin and wispy cultures of mycelium, which is what I was looking for! So, I believe I used an excessively dense nutrient recipe for the species causing a case of mistaken identity. I hit the trifecta for growing mold accidentally on purpose lol.

Live and learn. I need to pour some plates anyway so I'm going to pour a couple sleeves of some 'weak' plates for pans, omitting yeast and pour a sleeve or two of my regular cube plates.

Also, since I'm starting over with my entire LAGM project, I think I'm going to take this opportunity and do the entire process from pouring plates to inoculating jars in front of my shitty flow-hood. This will be a good test to see if it's actually usable. I know it's turbulent so far from ideal but if it's usable I may as well use the damn thing!


--------------------



I :heart: 5318008


NOT a virgin!


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InvisibleReverendMyc

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Re: LAGM v. 2.024 [Re: Noncense]
    #28626189 - 01/18/24 04:59 PM (9 days, 14 hours ago)

2024-01-18
Home for a few days so checking in.


Here is the whole gang including both germs plates and the last 4 are t1s. Looks about time to get t1s from one of the Mexican Chicon Nindo and both of the Subtropicalis Hindsight. Still no action on the subtrop 0t0lerance and xica but I am patient.


The subtropicalis bell cap t1 plates already look really nice and may get a t2 and sent to grain soon. The pan bunnell are not as pretty but may get the same treatment because in my VERY limited experience with pans, sometimes the ugly plates out perform the pretty ones.

OP updated


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Offlinemnj
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28626227 - 01/18/24 05:22 PM (9 days, 14 hours ago)

In solidarity with bigfootscreepyuncl I'll turn LAGM into Cultivation Confessional and add my minor update:

My original AA+ plate had only germinated in one spot as a bright white fuzzy mass that reached as high as it did wide. Took transfers to a few plates anyway to see what was up (because the Oak Ridge I did last year also started out growing very vertical on the germ plates but performed super well!). Anyway, the two T1 plates are growing fast and fuzzy and I don't trust em. Today I put em in a ziploc and then a Tupperware and set em aside to see what happens. Either I've got two dope mold plates or I'll have plins in mid February. My gut says mold.

Also I ditched my PE because it was a translucent top crawler and overall the agar it touched looked gross. Idk wtf it was but it wasn't worth my time.

I've already noc'd up a few fresh AA+ plates bc I've never grown this variety and wanna give it a go.

The PE I'm ditching for LAGM bc I already have a few clone cultures going. Might do some top fruited cakes if I want more options but that won't be part of this thread.

Keep at it creepyuncl, I know how excited you are to run exotics :rockon:


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mnj]
    #28626298 - 01/18/24 06:07 PM (9 days, 13 hours ago)

Quote:

mnj said:
In solidarity with bigfootscreepyuncl I'll turn LAGM into Cultivation Confessional and add my minor update:

My original AA+ plate had only germinated in one spot as a bright white fuzzy mass that reached as high as it did wide. Took transfers to a few plates anyway to see what was up (because the Oak Ridge I did last year also started out growing very vertical on the germ plates but performed super well!). Anyway, the two T1 plates are growing fast and fuzzy and I don't trust em. Today I put em in a ziploc and then a Tupperware and set em aside to see what happens. Either I've got two dope mold plates or I'll have plins in mid February. My gut says mold.

Also I ditched my PE because it was a translucent top crawler and overall the agar it touched looked gross. Idk wtf it was but it wasn't worth my time.

I've already noc'd up a few fresh AA+ plates bc I've never grown this variety and wanna give it a go.

The PE I'm ditching for LAGM bc I already have a few clone cultures going. Might do some top fruited cakes if I want more options but that won't be part of this thread.

Keep at it creepyuncl, I know how excited you are to run exotics :rockon:





Oh yeah, Pans got me down but not out :rockon: this heavyweight match is going all 12 rounds boi!

It sucks you're experiencing difficulties but this is literally what LAGM was created for. Help beginners learn agar and show them that mistakes are inevitable and everyone, regardless of experience strikes out every now and then. Keep your stick on the ice mnj :rockon:

AA+ is a fun variety, not too picky about conditions and readily throws canopies. Be sure to harvest before spore drop because they drop a LOT of spores ALL at once. If your new AA+ plates go tits up hit me up I've got some clean prints I'd be happy to share and a bangin' PE culture to boot.



So far everyone except me and mnj are doing pretty good :lol: keep up the good work everyone!


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Offlinemnj
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28626323 - 01/18/24 06:23 PM (9 days, 13 hours ago)

Yah man we got this

Just checked the AA+ and Pan Cambo Thai plates I noc'd up on Sunday and I've got itty bitty germination happening :laugh:

Updates to follow within the next week

Edit: I'm gonna run with what I have but many thanks for the offer on prints. Solid.


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


Edited by mnj (01/18/24 06:26 PM)


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Offlinemycosispeon
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Re: LAGM v. 2.024 [Re: mycosispeon] * 1
    #28626369 - 01/18/24 06:51 PM (9 days, 12 hours ago)

OP updated. T1s taken!


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Invisibleoxo
oxo

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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28627583 - 01/19/24 06:15 PM (8 days, 13 hours ago)

Quote:

mycosispeon said:
OP updated. T1s taken!



What are the little blips on the T1 plates?


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InvisibleMojaveMyc
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Re: LAGM v. 2.024 [Re: oxo] * 1
    #28627616 - 01/19/24 06:31 PM (8 days, 13 hours ago)

OP Updated.



The pans are on grain. Gandalf needs a little love & I’m still unsure about the pollock plate. If it turns out to be mold I’ll be pulling out those lovely bell cap semps :cheers:


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Offlinemycosispeon
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Re: LAGM v. 2.024 [Re: oxo]
    #28627712 - 01/19/24 08:02 PM (8 days, 11 hours ago)

Quote:

oxo said:
Quote:

mycosispeon said:
OP updated. T1s taken!



What are the little blips on the T1 plates?



Condensation. I've had the dishes stored upside-down and when I went to use them, I let as much of it drip out without fucking with it too much. I've had them stored upside-down since and now all the remaining condensation has be re-absorbed.


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Invisibleoxo
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Re: LAGM v. 2.024 [Re: mycosispeon]
    #28627724 - 01/19/24 08:06 PM (8 days, 11 hours ago)

Quote:

mycosispeon said:
Quote:

oxo said:
Quote:

mycosispeon said:
OP updated. T1s taken!



What are the little blips on the T1 plates?



Condensation. I've had the dishes stored upside-down and when I went to use them, I let as much of it drip out without fucking with it too much. I've had them stored upside-down since and now all the remaining condensation has be re-absorbed.



Interesting, thanks.


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OfflineSeventhMushroom
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28627847 - 01/19/24 09:39 PM (8 days, 10 hours ago)

Quote:

bigfootscreepyuncl said:
Also, since I'm starting over with my entire LAGM project, I think I'm going to take this opportunity and do the entire process from pouring plates to inoculating jars in front of my shitty flow-hood. This will be a good test to see if it's actually usable. I know it's turbulent so far from ideal but if it's usable I may as well use the damn thing!




What's so shitty about your flow-hood?? And how do you know it's turbulent?


--------------------
LAGM 2024


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: SeventhMushroom]
    #28627890 - 01/19/24 10:20 PM (8 days, 9 hours ago)

Quote:

SeventhMushroom said:
Quote:

bigfootscreepyuncl said:
Also, since I'm starting over with my entire LAGM project, I think I'm going to take this opportunity and do the entire process from pouring plates to inoculating jars in front of my shitty flow-hood. This will be a good test to see if it's actually usable. I know it's turbulent so far from ideal but if it's usable I may as well use the damn thing!




What's so shitty about your flow-hood?? And how do you know it's turbulent?





Well, a friend of mine has a mushroom farm and he was upgrading so I traded a friend of mine a few ounces of PE for it. He took shit care of it once he got in his 3 new meyers 2x4's. All of the aluminum fins on the filter are bent and mangled. One quadrant of the filter has holes in it deep in the pleats so I can't find them to seal them, but I know they're there because every time I test the filter with plates that quadrant contaminates every single time. But, the other 3 quadrants don't.

I know it's turbulent because I took the blower out thinking I'd just get a new filter and build a new, better one. After running the numbers dozens of times the math just doesn't line up at all. The motor is WAY too powerful for the 2x4 filter I have. To have true laminar flow I'd probably need like a 3x6 or a 2x8 filter for the blower motor I have. It'll blow out a lighter from like 2 feet away lol.

BUT, I have been playing with it recently. I've done some agar transfers in front of it and they haven't contaminated yet and I inoculated 4 jars of grain and they're looking great. So, despite being able to use only 75% of the filter and knowing that it's turbulent I think it will still be usable for the little bit of work I'll do in front of it.


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I :heart: 5318008


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OfflineSeventhMushroom
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28628675 - 01/20/24 03:04 PM (7 days, 16 hours ago)

A hack you might try, if the blower is too powerful, rather than replace anything, just drill/poke a (small) hole in the side of the box to relieve the pressure before the filter. Make the hole larger until it is no longer turbulent. Wasted energy? sure, but if you can bleed off enough of the pressure, you can get the flow rate down to a more acceptable level. From my research, there should be a large acceptable range from ~40FPM - ~120FPM velocity, and lower is probably better. And hey, if it doesn't work you can always tape over the holes again. Just my 2 cents.


--------------------
LAGM 2024


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OfflineSeventhMushroom
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Re: LAGM v. 2.024 [Re: SeventhMushroom] * 1
    #28628712 - 01/20/24 03:25 PM (7 days, 16 hours ago)

On another note, OP updated with the status of my LC. Looks like it's doing okay so far!



--------------------
LAGM 2024


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Offlinemnj
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Re: LAGM v. 2.024 [Re: SeventhMushroom]
    #28629432 - 01/21/24 05:35 AM (7 days, 2 hours ago)

That's a happy update SeventhMushroom :cool::mushroom2:


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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OfflineCaptainPuffy
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Re: LAGM v. 2.024 [Re: mnj]
    #28630389 - 01/21/24 08:03 PM (6 days, 11 hours ago)

OP updated

Ended up with a couple decent transfers and a lot of plates showing bacteria. Still salvageable.

I know the consensus of the community is to use  soapy water, but I seem to be having a higher rate of bacterial contamination from just soapy water instead of 70% Iso.

I have to be extremely strict with my clean procedure with soapy water. I believe my point of failure this time was that I showered in the morning and made my transfers in the evening. In the past when I used 70% iso I didn't have to be so strict. Just wash my arms really well and make sure I showered at some point that day.

I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: CaptainPuffy]
    #28630410 - 01/21/24 08:21 PM (6 days, 11 hours ago)

Quote:

CaptainPuffy said:
I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.





Scrub my arms like I'm prepping for surgery, gloves, hat, covid mask. Iso forearms and gloved hands. Never spray any liquids on the floor of my SAB and I don't use one of those cooling sheets, I just use blank plates as a 'false bottom' to keep everything elevated off the SAB floor. I shower every day but it rarely aligns itself with my SAB session and I pay no attention to it.


--------------------



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OfflineCaptainPuffy
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Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28630423 - 01/21/24 08:34 PM (6 days, 11 hours ago)

Quote:

bigfootscreepyuncl said:
Quote:

CaptainPuffy said:
I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.





Scrub my arms like I'm prepping for surgery, gloves, hat, covid mask. Iso forearms and gloved hands. Never spray any liquids on the floor of my SAB and I don't use one of those cooling sheets, I just use blank plates as a 'false bottom' to keep everything elevated off the SAB floor. I shower every day but it rarely aligns itself with my SAB session and I pay no attention to it.




Thanks for the explanation! I definitely scrub/ISO my arms and wear gloves and a mask. I'll try running a dry SAB next time and see what happens


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OfflineVibetyme
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Re: LAGM v. 2.024 [Re: CaptainPuffy] * 2
    #28630567 - 01/22/24 12:24 AM (6 days, 7 hours ago)

For all my shoebox growers out there who have had issues with fungus gnats. I think I came across a permanent solution.

The wife loves her indoor plants and we had a fungus gnat problem during the summer.  I tried various things to get rid of them, like the vinegar & dish soap traps, a fan/uv light/sticky trap combo called the katchy, and various other sticky traps.
They all kinda worked but didn't really take care of the issue 100%.

I started thinking about a bag to put my tubs in that would let light and air in but keep fungus gnats the hell out.  I got on walmart.com and put my plan into motion. I ordered some of these fine mesh laundry bags.



The holes are so small the gnats can't get in.

The ones I ordered are a little bigger then my shoeboxes, but you could probably find some bigger ones if you search.
At $2.97 per bag they are a hell of a deal if you have ever dealt with those little fucks.

I'm also gonna make a post about this in the main mushroom cultivation thread cuz the people need to know about this.


--------------------
Vibetyme's LAGM 2.022

Vibetyme's LAGM_23

Vibetyme's LAGM_24

                 


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Re: LAGM v. 2.024 [Re: Vibetyme] * 1
    #28630589 - 01/22/24 01:17 AM (6 days, 6 hours ago)

We have lift off on all them thangs! Super stoked about these varieties! 
Toque ,Albino Riptide, and Starry Night Ape


--------------------
Vibetyme's LAGM 2.022

Vibetyme's LAGM_23

Vibetyme's LAGM_24

                 


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InvisibleFloret
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Re: LAGM v. 2.024 [Re: Vibetyme]
    #28630624 - 01/22/24 02:59 AM (6 days, 4 hours ago)

Quote:

CaptainPuffy said:
I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.




I have a towel with a raised grate on the bottom of my SAB. I wipe down my jars and plates with iso, place them in my SAB and spray down the walls with a fine mist of soapy water. I unscrew everything and prep it all to get ready to work then leave and let the air settle. I wash my hands before entering the room then spritz then with soapy water before starting my session. SAB sessions don't dictate when I shower.

I know I technically don't need to spritz my hands before, spray the walls of the box, or wipe anything with iso but it doesn't take long and gives me peace of mind so I continue to do it.

Quote:

Vibetyme said:
For all my shoebox growers out there who have had issues with fungus gnats. I think I came across a permanent solution.




I mix mosquito bits into my pseudo casing. Others spray with BT (same thing, check that it targets gnats). It's food safe.


--------------------
LAGM 2024


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Re: LAGM v. 2.024 [Re: CaptainPuffy]
    #28630643 - 01/22/24 03:59 AM (6 days, 3 hours ago)

Quote:

CaptainPuffy said:
I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.




I just spray a little soapy water, wash my hands, put on gloves and disinfect them then go to work immediately. Sometimes I even forego the gloves.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisibleOctopusDisco
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Re: LAGM v. 2.024 [Re: Noncense]
    #28630834 - 01/22/24 09:33 AM (5 days, 22 hours ago)

I know it's a bit late, but I'm a newcomer to the hobby and was hesitant to post here.

Background

I realized I had made far too many plates after my second agar attempt and decided to get a bit more out of the GT MSS I used to get started in this hobby back in November. Even though I emptied the syringe as much as I could, I figured there would still be a sufficient number of spores attached to the plunger, so I sterilized some water and sucked around 20mls back into the barrel. This is the spore solution that I will use :smile:

For LAGM 2024, the plan is to:
1) Germinate spores on normal agar
2) Leave the cultures until they pin
3) Clone all fully-formed fruits to normal agar
4) T1s to water agar, using a fairly large donor sample (I have a hunch that this will help the mycelium stretch out a bit to aid in obtaining a clean sample)
5) T2s to soft agar (at this point, I'm assuming the culture from 3 is clean)
6) Soft agar to grain
7) Spawn to bulk in [TYPE OF CONTAINER TO BE DETERMINED]
8) Take prints from interesting fruits from each culture
9) Repeat steps 1 to 8 until 2025


LAGM 2024

1) Germinate spores on normal agar

I put far too much spore solution on the first couple of sauce containers, but I got the technique down such that I would suspend a drop of solution from the needle tip and then apply to the surface of the agar. I put 4 drops on the 90mm petri--one in each quadrant.

Photo 1 (240122): 4 sauce containers with soft LME agar ("S"), 4 sauce containers with normal LME agar ("H"), and 1 90mm petri dish with normal grain water agar--all with GT from MSS on 12/29.

Photo 2 (240122): 90mm petri dish with 4 inoculation points. Notice the distinct boundaries between established colonies.



2) Leave the cultures until they pin
3) Clone all fully-formed fruits to normal agar
4) T1s to water agar, using a fairly large donor sample (I have a hunch that this will help the mycelium stretch out a bit to aid in obtaining a clean sample)
5) T2s to soft agar (at this point, I'm assuming the culture from 3 is clean)
6) Soft agar to grain
7) Spawn to bulk in [TYPE OF CONTAINER TO BE DETERMINED]
8) Take prints from interesting fruits from each culture
9) Repeat steps 1 to 8 until 2025


--------------------
LAGM 2.024

Serial system ID data


Edited by OctopusDisco (01/22/24 10:40 AM)


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Offlinemnj
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Re: LAGM v. 2.024 [Re: OctopusDisco]
    #28630853 - 01/22/24 09:58 AM (5 days, 21 hours ago)

Hey OctopusDisco Welcome Welcome

Glad you're jumping in.  I know from your log that you have a scientific mind.  It'll be cool to watch your progress.

Have fun :mushroom2:


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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Re: LAGM v. 2.024 [Re: mnj]
    #28630962 - 01/22/24 11:45 AM (5 days, 19 hours ago)

OP updated. T1s are colonizing nicely


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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InvisibleOctopusDisco
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Re: LAGM v. 2.024 [Re: mnj] * 1
    #28631110 - 01/22/24 02:16 PM (5 days, 17 hours ago)

Quote:

mnj said:
Hey OctopusDisco Welcome Welcome

Glad you're jumping in.  I know from your log that you have a scientific mind.  It'll be cool to watch your progress.

Have fun :mushroom2:




Thanks, friend! I'm super stoked to see how your Pans turn out. I also love the detailed process and technique notes in your log for LAGM. For posterity's sake, I will make a point to do the same.

Yew!

:sporedrop:


--------------------
LAGM 2.024

Serial system ID data


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OfflineAndImTheHighOne
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Re: LAGM v. 2.024 [Re: OctopusDisco] * 1
    #28631448 - 01/22/24 07:55 PM (5 days, 11 hours ago)

Well, that was terrible... By far my sloppiest SAB session since the first time I tried using agar, maybe even more so. Really pretty unhappy with myself. I should have stopped when I first felt myself rushing and getting worked up by mistakes that normally wouldn't bother me so much. I decided to power through, and ended up making even more, bigger mistakes. Shocking!

So I'm going to wait a couple of days for my next picture update. I'll be holding my breath that the rest of my T2s aren't completely fucked.


--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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Re: LAGM v. 2.024 [Re: AndImTheHighOne] * 1
    #28631888 - 01/23/24 09:03 AM (4 days, 22 hours ago)

I up voted your post because I feel your pain, not because I approve of it. We have all been there and it is often not as bad as you think. But you can always get a redo if needed.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Re: LAGM v. 2.024 [Re: ReverendMyc] * 4
    #28631947 - 01/23/24 09:50 AM (4 days, 21 hours ago)

Quote:

ReverendMyc said:
I up voted your post because I feel your pain, not because I approve of it. We have all been there and it is often not as bad as you think. But you can always get a redo if needed.




19 years on the shroomery and I jut learned you can upvote posts :flowstone:


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28632033 - 01/23/24 11:07 AM (4 days, 20 hours ago)

I feel your pain, too AndImTheHighOne.  Been there.  Sounds like you already know what your issues were so come back to the SAB when you're feelin good and ready to groove with it :thumbup:


Gastronomicus I think it took me over a decade to notice :lol:


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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Re: LAGM v. 2.024 [Re: Gastronomicus]
    #28632037 - 01/23/24 11:14 AM (4 days, 20 hours ago)



I wonder if this Natalensis plate is grown enough to take T1s from. All my recent plates contaminated with the small irregular spots (seen better in the bottom picture) before they grew anything, and this plate is the only one that has visible mycelium, the other ones still have nothing or that small spot contamination only.

It seems to me that the area right in front of the swab tip is the best, there is none of the small spot contaminate there and I can probably get enough transfers out of there if I use the needle poke tek. The other bigger spots of growth should also be mycelium, the swab got loose a couple days after inoculation and rolled around for a bit before I could stop it in a stable position. The mycelium seems to be going into the agar, which wasn't the case with the cube plates I had going earlier, I wonder if Natalensis is known to do this.

The weather turned colder suddenly and that's probably affecting my plates as well. I have 2 shelves I can use for my projects, one of them gets too cold, some of the time it gets down to 57 and the average temps are around 60. The other one is higher in the room and all the warm air goes there, so it's close to too hot almost all the time with average temps of 77-80. Hopefully this will be over soon because I can't do anything else to control the temperature at the moment.


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InvisibleReverendMyc

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Re: LAGM v. 2.024 [Re: ReverendMyc]
    #28632065 - 01/23/24 11:48 AM (4 days, 19 hours ago)

Did some things a few days ago then went back on the road.

Quote:

ReverendMyc said:
2024-01-19


t2 from the pan bunnells and t1 from one Mex Chicon Nindo and two Subtropicalis Hindsight. Going to do t2 transfers from the subtropicalis when I put them these plates to grain in a couple of days if the look worthy. t1 is usually too early for that, but I don't mind wasting a couple of jars on a chance.

Also started some Golden Teachers from prints to plates and prints to swabs to pucks for another couple of projects.

Its a grow off!
Plates vs pucks.
Used a print to do plates using a loop and two swabs dipped in sterile water and wiped on print to pucks. Lets see what happens.






--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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OfflineAndImTheHighOne
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Re: LAGM v. 2.024 [Re: ReverendMyc] * 2
    #28632171 - 01/23/24 02:09 PM (4 days, 17 hours ago)

Thanks you guys! I really appreciate the encouraging words, Reverend and mnj. It is reassuring to know that it just goes like that sometimes.

I couldn't bring myself to even look at the plates last night. I just threw em on my little shelf, wrote out my postmortem in my journal, and went to bed. Getting a chance to scrutinize them this morning without all the added emotion, none were as bad as I remembered while doing them. The mind is a funny thing sometimes.

So, I'm still holding my breath, but I'm at peace with it all. If I have to start over on a few it won't be the end of the world. I have more MS solution of each of those strains except one, which I've already grown out on cakes and have my own spore print. So it's all good!


--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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OfflineSirPsycho
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Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28632948 - 01/24/24 07:31 AM (4 days, 9 minutes ago)

Ain't shit happening with my first inoculation attempt so I'm gonna try again with the same wild Florida print (left) and a wild Alabama print (right). Both cubes



--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

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Re: LAGM v. 2.024 [Re: SirPsycho]
    #28633047 - 01/24/24 08:52 AM (3 days, 22 hours ago)

Was wondering how your plate was going SirPsycho.  Hope these get liftoff :rocket:

Updated me log

Took T1's and also made some "EF/Pan-Cakes/Bottle Tek/PF for EZ Cloning" cakes bc my pan syringes have proven themselves to be contam-free on my LAGM plates.


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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OfflineSirPsycho
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Re: LAGM v. 2.024 [Re: mnj]
    #28633096 - 01/24/24 09:37 AM (3 days, 22 hours ago)

Quote:

mnj said:
Was wondering how your plate was going SirPsycho.  Hope these get liftoff :rocket:

Updated me log

Took T1's and also made some "EF/Pan-Cakes/Bottle Tek/PF for EZ Cloning" cakes bc my pan syringes have proven themselves to be contam-free on my LAGM plates.



I think erythrozine (red#3) finally bit me in the ass. (shhh, don't tell rumfor69) All the plates from my last batch of agar looked weak AF... Except the two pan cyan cultures I got going.

Made fresh plates yesterday and used the plates with condensation for this inoculation


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Re: LAGM v. 2.024 [Re: SirPsycho] * 1
    #28633127 - 01/24/24 10:10 AM (3 days, 21 hours ago)

Quote:

SirPsycho said:

I think erythrozine (red#3) finally bit me in the ass.




I think it bit me in the ass on a round of plates last year.  It had been years since I did agar so I had totally forgotten about the red dye thing.  Anyway, the blue plates did well but the red ones didn't, then it dawned on me...


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mnj] * 3
    #28633142 - 01/24/24 10:23 AM (3 days, 21 hours ago)

Well I haven't done a goddamn thing for my LAGM lately but I'll be out of town for a while so I need to get my ass in gear. I was told by a few experts in the field that my plates are not actually moldy but Pan cyan mycelium just looks like shit, so... :shrug:

Guess I'll transfer what I'm still not entirely convinced isn't mold and see what comes of it lol.

This species has already humbled me..I definitely feel like a scared noob all over again, which isn't all too different from my default arrogant noob mentality :lol:


--------------------



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Re: LAGM v. 2.024 [Re: mnj] * 3
    #28633156 - 01/24/24 10:38 AM (3 days, 21 hours ago)

Well I've been procrastinating working and posting but here's an update.

T1 transfers were looking really good, I was considering sending them but some small contams / sus parts started showing up so I took some T2's

Small rings on these two



Little nibble out of the side on this one



This pink boi




Still, the myc looks happy so hopefully I get something clean enough to put on grain with the T2's


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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InvisibleReverendMyc

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Re: LAGM v. 2.024 [Re: ReverendMyc]
    #28633627 - 01/24/24 06:03 PM (3 days, 13 hours ago)

Quote:

ReverendMyc said:
2024-01-24
Home again so update time. Started prepping some grain for the subtropicalis bell cap t1s and 15 other non-lagm plates that are about ready.
Grabbed update pix of transfer plates.


pan bunnell t1 and t2.


mexicana chicon nindo and subtropicalis t1s


golden teacher nothing to see yet

Plan to get a t2 and put these t1 subtrop bell caps to grain tomorrow when the jars finish cooling.





--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Re: LAGM v. 2.024 [Re: ReverendMyc] * 1
    #28634258 - 01/25/24 09:59 AM (2 days, 21 hours ago)

OP updated

It was time to make T2s of the Ghost. The TAT is lagging a bit and I ended up deciding to make some new T1s.


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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InvisibleReverendMyc

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Re: LAGM v. 2.024 [Re: ReverendMyc] * 1
    #28635037 - 01/25/24 09:29 PM (2 days, 10 hours ago)

2024-01-23
I actually executed the plan. Don't tell my wife that I can do that.


subtropicalis bell cap went to t2s and 2x1 pint millet grain jars.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Onlinefiddle_headS
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Re: LAGM v. 2.024 [Re: Noncense] * 1
    #28635130 - 01/26/24 12:25 AM (2 days, 7 hours ago)

little bit late to the party but im joining up here, gonna do

ps. semperviva
ps. cubensis var gandalf
ps. cubensis var makilla gorilla
ps. cubensis var fp+
and a load of other unique cube varieties including toque
pleurotus ostreatus ¨black king pearl¨
ganoderma lucidum

gonna be swabbing these tonight to kick things off:


:raver2::rave::raver2:

ps: if i got a swab or a culture that came from a swab i was given, you already know who you are and what you mean to me.

looking forward to seeing what everyones got going


Edited by fiddle_head (01/26/24 12:29 AM)


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Offlineedixo
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Re: LAGM v. 2.024 [Re: edixo]
    #28635156 - 01/26/24 01:28 AM (2 days, 6 hours ago)

Quote:

edixo said:
26/01

Grain transfers are slowly coming in. However, something else has happened - I accidentally dropped one of the transfer plates while looking at it, which caused the transfer wedge to split into three. Two stayed in the middle, and another went to the edge of the plate. Some bacterial contamination sprung up right next to it, and lo and behold; the myc beat it. It's slowly surrounding the bacteria, and the myc growing from it is markedly thicker and more even than the other wedge pieces.

I think I'm gonna isolate it.






--------------------
~ LAGM 2024 ~


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Offlineedixo
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Re: LAGM v. 2.024 [Re: edixo]
    #28636391 - 01/27/24 01:48 AM (1 day, 5 hours ago)

Quote:

edixo said:
27/01

Not even 24 hours after transferring that contam resistant strain, and it looks like this. Exciting!






--------------------
~ LAGM 2024 ~


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Re: LAGM v. 2.024 [Re: edixo]
    #28636839 - 01/27/24 11:53 AM (19 hours, 47 minutes ago)

Updated OP

The original T1 of TAT has one "cord" that is breaking away from the rest. Is that just a rogue strain? Or two that joined together?

These pix are 4 days apart



--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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Re: LAGM v. 2.024 [Re: mycosispeon] * 1
    #28636957 - 01/27/24 01:43 PM (17 hours, 57 minutes ago)

This is such a great question. I'm leaning toward the possibility that they joined together from my visual interpretation, but I don't have much knowledge or experience of how mycelium acts, so take my opinion for what it's worth, which is probably not much.

:cheers:


--------------------
LAGM 2024

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Thank yoU
please come again
FrienD


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Re: LAGM v. 2.024 [Re: mnj]
    #28636968 - 01/27/24 02:01 PM (17 hours, 39 minutes ago)

:popcorn:


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