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InvisibleFloret
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Registered: 01/02/23
Posts: 260
Loc: underneath the water
Re: LAGM v. 2.024 [Re: Vibetyme]
    #28630624 - 01/22/24 02:59 AM (6 days, 4 hours ago)

Quote:

CaptainPuffy said:
I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.




I have a towel with a raised grate on the bottom of my SAB. I wipe down my jars and plates with iso, place them in my SAB and spray down the walls with a fine mist of soapy water. I unscrew everything and prep it all to get ready to work then leave and let the air settle. I wash my hands before entering the room then spritz then with soapy water before starting my session. SAB sessions don't dictate when I shower.

I know I technically don't need to spritz my hands before, spray the walls of the box, or wipe anything with iso but it doesn't take long and gives me peace of mind so I continue to do it.

Quote:

Vibetyme said:
For all my shoebox growers out there who have had issues with fungus gnats. I think I came across a permanent solution.




I mix mosquito bits into my pseudo casing. Others spray with BT (same thing, check that it targets gnats). It's food safe.


--------------------
LAGM 2024


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OfflineTiamo
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Re: LAGM v. 2.024 [Re: CaptainPuffy]
    #28630643 - 01/22/24 03:59 AM (6 days, 3 hours ago)

Quote:

CaptainPuffy said:
I'd love to hear how others are using their SAB. Do you shower immediately before? Do you use ISO, Soapy Water, or a Dry SAB? Any suggestions are welcome.




I just spray a little soapy water, wash my hands, put on gloves and disinfect them then go to work immediately. Sometimes I even forego the gloves.


--------------------


If you have used a Miraculix Psilocybin QTest, could you please share your results?

Shipping free Ps. natalensis spore prints to any address in The Netherlands, just :pm:

:mushroom2: Mush love :mushroom2:


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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
Loc: In a bear hole
Re: LAGM v. 2.024 [Re: Noncense]
    #28630834 - 01/22/24 09:33 AM (5 days, 22 hours ago)

I know it's a bit late, but I'm a newcomer to the hobby and was hesitant to post here.

Background

I realized I had made far too many plates after my second agar attempt and decided to get a bit more out of the GT MSS I used to get started in this hobby back in November. Even though I emptied the syringe as much as I could, I figured there would still be a sufficient number of spores attached to the plunger, so I sterilized some water and sucked around 20mls back into the barrel. This is the spore solution that I will use :smile:

For LAGM 2024, the plan is to:
1) Germinate spores on normal agar
2) Leave the cultures until they pin
3) Clone all fully-formed fruits to normal agar
4) T1s to water agar, using a fairly large donor sample (I have a hunch that this will help the mycelium stretch out a bit to aid in obtaining a clean sample)
5) T2s to soft agar (at this point, I'm assuming the culture from 3 is clean)
6) Soft agar to grain
7) Spawn to bulk in [TYPE OF CONTAINER TO BE DETERMINED]
8) Take prints from interesting fruits from each culture
9) Repeat steps 1 to 8 until 2025


LAGM 2024

1) Germinate spores on normal agar

I put far too much spore solution on the first couple of sauce containers, but I got the technique down such that I would suspend a drop of solution from the needle tip and then apply to the surface of the agar. I put 4 drops on the 90mm petri--one in each quadrant.

Photo 1 (240122): 4 sauce containers with soft LME agar ("S"), 4 sauce containers with normal LME agar ("H"), and 1 90mm petri dish with normal grain water agar--all with GT from MSS on 12/29.

Photo 2 (240122): 90mm petri dish with 4 inoculation points. Notice the distinct boundaries between established colonies.



2) Leave the cultures until they pin
3) Clone all fully-formed fruits to normal agar
4) T1s to water agar, using a fairly large donor sample (I have a hunch that this will help the mycelium stretch out a bit to aid in obtaining a clean sample)
5) T2s to soft agar (at this point, I'm assuming the culture from 3 is clean)
6) Soft agar to grain
7) Spawn to bulk in [TYPE OF CONTAINER TO BE DETERMINED]
8) Take prints from interesting fruits from each culture
9) Repeat steps 1 to 8 until 2025


--------------------
LAGM 2.024

Serial system ID data


Edited by OctopusDisco (01/22/24 10:40 AM)


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Offlinemnj
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Re: LAGM v. 2.024 [Re: OctopusDisco]
    #28630853 - 01/22/24 09:58 AM (5 days, 21 hours ago)

Hey OctopusDisco Welcome Welcome

Glad you're jumping in.  I know from your log that you have a scientific mind.  It'll be cool to watch your progress.

Have fun :mushroom2:


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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Offlinemycosispeon
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Re: LAGM v. 2.024 [Re: mnj]
    #28630962 - 01/22/24 11:45 AM (5 days, 19 hours ago)

OP updated. T1s are colonizing nicely


--------------------
LAGM 2.024

- I'm new so take everything I say with a grain of salt :wink:



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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
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Re: LAGM v. 2.024 [Re: mnj] * 1
    #28631110 - 01/22/24 02:16 PM (5 days, 17 hours ago)

Quote:

mnj said:
Hey OctopusDisco Welcome Welcome

Glad you're jumping in.  I know from your log that you have a scientific mind.  It'll be cool to watch your progress.

Have fun :mushroom2:




Thanks, friend! I'm super stoked to see how your Pans turn out. I also love the detailed process and technique notes in your log for LAGM. For posterity's sake, I will make a point to do the same.

Yew!

:sporedrop:


--------------------
LAGM 2.024

Serial system ID data


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OfflineAndImTheHighOne
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Re: LAGM v. 2.024 [Re: OctopusDisco] * 1
    #28631448 - 01/22/24 07:55 PM (5 days, 11 hours ago)

Well, that was terrible... By far my sloppiest SAB session since the first time I tried using agar, maybe even more so. Really pretty unhappy with myself. I should have stopped when I first felt myself rushing and getting worked up by mistakes that normally wouldn't bother me so much. I decided to power through, and ended up making even more, bigger mistakes. Shocking!

So I'm going to wait a couple of days for my next picture update. I'll be holding my breath that the rest of my T2s aren't completely fucked.


--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: LAGM v. 2.024 [Re: AndImTheHighOne] * 1
    #28631888 - 01/23/24 09:03 AM (4 days, 22 hours ago)

I up voted your post because I feel your pain, not because I approve of it. We have all been there and it is often not as bad as you think. But you can always get a redo if needed.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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OfflineGastronomicus
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Re: LAGM v. 2.024 [Re: ReverendMyc] * 4
    #28631947 - 01/23/24 09:50 AM (4 days, 21 hours ago)

Quote:

ReverendMyc said:
I up voted your post because I feel your pain, not because I approve of it. We have all been there and it is often not as bad as you think. But you can always get a redo if needed.




19 years on the shroomery and I jut learned you can upvote posts :flowstone:


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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Offlinemnj
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Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28632033 - 01/23/24 11:07 AM (4 days, 20 hours ago)

I feel your pain, too AndImTheHighOne.  Been there.  Sounds like you already know what your issues were so come back to the SAB when you're feelin good and ready to groove with it :thumbup:


Gastronomicus I think it took me over a decade to notice :lol:


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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InvisibleLyleChipperson


Registered: 09/29/23
Posts: 61
Re: LAGM v. 2.024 [Re: Gastronomicus]
    #28632037 - 01/23/24 11:14 AM (4 days, 20 hours ago)



I wonder if this Natalensis plate is grown enough to take T1s from. All my recent plates contaminated with the small irregular spots (seen better in the bottom picture) before they grew anything, and this plate is the only one that has visible mycelium, the other ones still have nothing or that small spot contamination only.

It seems to me that the area right in front of the swab tip is the best, there is none of the small spot contaminate there and I can probably get enough transfers out of there if I use the needle poke tek. The other bigger spots of growth should also be mycelium, the swab got loose a couple days after inoculation and rolled around for a bit before I could stop it in a stable position. The mycelium seems to be going into the agar, which wasn't the case with the cube plates I had going earlier, I wonder if Natalensis is known to do this.

The weather turned colder suddenly and that's probably affecting my plates as well. I have 2 shelves I can use for my projects, one of them gets too cold, some of the time it gets down to 57 and the average temps are around 60. The other one is higher in the room and all the warm air goes there, so it's close to too hot almost all the time with average temps of 77-80. Hopefully this will be over soon because I can't do anything else to control the temperature at the moment.


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: LAGM v. 2.024 [Re: ReverendMyc]
    #28632065 - 01/23/24 11:48 AM (4 days, 19 hours ago)

Did some things a few days ago then went back on the road.

Quote:

ReverendMyc said:
2024-01-19


t2 from the pan bunnells and t1 from one Mex Chicon Nindo and two Subtropicalis Hindsight. Going to do t2 transfers from the subtropicalis when I put them these plates to grain in a couple of days if the look worthy. t1 is usually too early for that, but I don't mind wasting a couple of jars on a chance.

Also started some Golden Teachers from prints to plates and prints to swabs to pucks for another couple of projects.

Its a grow off!
Plates vs pucks.
Used a print to do plates using a loop and two swabs dipped in sterile water and wiped on print to pucks. Lets see what happens.






--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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OfflineAndImTheHighOne
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Re: LAGM v. 2.024 [Re: ReverendMyc] * 2
    #28632171 - 01/23/24 02:09 PM (4 days, 17 hours ago)

Thanks you guys! I really appreciate the encouraging words, Reverend and mnj. It is reassuring to know that it just goes like that sometimes.

I couldn't bring myself to even look at the plates last night. I just threw em on my little shelf, wrote out my postmortem in my journal, and went to bed. Getting a chance to scrutinize them this morning without all the added emotion, none were as bad as I remembered while doing them. The mind is a funny thing sometimes.

So, I'm still holding my breath, but I'm at peace with it all. If I have to start over on a few it won't be the end of the world. I have more MS solution of each of those strains except one, which I've already grown out on cakes and have my own spore print. So it's all good!


--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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OfflineSirPsycho
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Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28632948 - 01/24/24 07:31 AM (4 days, 9 minutes ago)

Ain't shit happening with my first inoculation attempt so I'm gonna try again with the same wild Florida print (left) and a wild Alabama print (right). Both cubes



--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Offlinemnj
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Re: LAGM v. 2.024 [Re: SirPsycho]
    #28633047 - 01/24/24 08:52 AM (3 days, 22 hours ago)

Was wondering how your plate was going SirPsycho.  Hope these get liftoff :rocket:

Updated me log

Took T1's and also made some "EF/Pan-Cakes/Bottle Tek/PF for EZ Cloning" cakes bc my pan syringes have proven themselves to be contam-free on my LAGM plates.


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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OfflineSirPsycho
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Re: LAGM v. 2.024 [Re: mnj]
    #28633096 - 01/24/24 09:37 AM (3 days, 22 hours ago)

Quote:

mnj said:
Was wondering how your plate was going SirPsycho.  Hope these get liftoff :rocket:

Updated me log

Took T1's and also made some "EF/Pan-Cakes/Bottle Tek/PF for EZ Cloning" cakes bc my pan syringes have proven themselves to be contam-free on my LAGM plates.



I think erythrozine (red#3) finally bit me in the ass. (shhh, don't tell rumfor69) All the plates from my last batch of agar looked weak AF... Except the two pan cyan cultures I got going.

Made fresh plates yesterday and used the plates with condensation for this inoculation


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Offlinemnj
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Re: LAGM v. 2.024 [Re: SirPsycho] * 1
    #28633127 - 01/24/24 10:10 AM (3 days, 21 hours ago)

Quote:

SirPsycho said:

I think erythrozine (red#3) finally bit me in the ass.




I think it bit me in the ass on a round of plates last year.  It had been years since I did agar so I had totally forgotten about the red dye thing.  Anyway, the blue plates did well but the red ones didn't, then it dawned on me...


--------------------
LAGM 2024

:sagetrip:

Thank yoU
please come again
FrienD


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Invisiblebigfootscreepyuncl
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Re: LAGM v. 2.024 [Re: mnj] * 3
    #28633142 - 01/24/24 10:23 AM (3 days, 21 hours ago)

Well I haven't done a goddamn thing for my LAGM lately but I'll be out of town for a while so I need to get my ass in gear. I was told by a few experts in the field that my plates are not actually moldy but Pan cyan mycelium just looks like shit, so... :shrug:

Guess I'll transfer what I'm still not entirely convinced isn't mold and see what comes of it lol.

This species has already humbled me..I definitely feel like a scared noob all over again, which isn't all too different from my default arrogant noob mentality :lol:


--------------------



I :heart: 5318008


NOT a virgin!


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OfflineGastronomicus
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Re: LAGM v. 2.024 [Re: mnj] * 3
    #28633156 - 01/24/24 10:38 AM (3 days, 21 hours ago)

Well I've been procrastinating working and posting but here's an update.

T1 transfers were looking really good, I was considering sending them but some small contams / sus parts started showing up so I took some T2's

Small rings on these two



Little nibble out of the side on this one



This pink boi




Still, the myc looks happy so hopefully I get something clean enough to put on grain with the T2's


--------------------
Make my Funk the P Funk, I wants to get Funked up

LAGM2024


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: LAGM v. 2.024 [Re: ReverendMyc]
    #28633627 - 01/24/24 06:03 PM (3 days, 13 hours ago)

Quote:

ReverendMyc said:
2024-01-24
Home again so update time. Started prepping some grain for the subtropicalis bell cap t1s and 15 other non-lagm plates that are about ready.
Grabbed update pix of transfer plates.


pan bunnell t1 and t2.


mexicana chicon nindo and subtropicalis t1s


golden teacher nothing to see yet

Plan to get a t2 and put these t1 subtrop bell caps to grain tomorrow when the jars finish cooling.





--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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