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OfflineSan Pedro GirlS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tittlez]
    #28591388 - 12/20/23 01:06 PM (1 month, 7 days ago)

Quote:

tittlez said:
Quote:

LadysKnight said:
Cook agar in a pot on the stove. Pour into plates on countertop, let cool. Add lids. Stack plates, wrap in foil, PC for 30. Done

Ime, boilovers only happen if you manually depressurize the PC.




Amazing!! That sounds like a plan :chugbeer:



I was less than pleased with my 100mm x 15mm non pour plates. If yours work out awesome, be sure to tell us exactly what you did.


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OfflineNichrome
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 1
    #28591545 - 12/20/23 03:28 PM (1 month, 7 days ago)

Quote:

tryptkaloids said:
Quote:

Nichrome said:
What % of nutrition in your mix? Across the board it just looks like a minor media resource imbalance of some kind but honestly not too bad.

This was just a phenomenon I noticed pulling cultures from cold storage over time so I started employing the occurrence on purpose to help with my own goals.


These are just my crazy thoughts so take it as you will. Grain of salt and all that. :laugh2:

:popcorn:



These plates are 10g agar which I usually dial back a little bit
And 9g potato flakes to 500ml

They came from lme, I don't remember the recipe but I usually run it at 7g for 500ml

Nothing else.

I'll definitely try an lc in cold storage. Should I let it grow out a little before the fridge or put it in there right after I make it?

It'll give me the time to wait for real plates to come in and work the other cultures on some no pours





Fully mature LC. Teaming with metabolites, and all the nutrition stored up in tissue is best.

No GE for cold storage. Try to leave as small of a bubble as possible so the oxygen gets used up and is not an inspirational factor for metabolic processes. leaving the bottled LC to sit at room temp for a couple days before the cold storage helps to use up the remaining oxygen. The goal is to slow metabolism to a near halt so they enter an induced meditative state. Light, heat, oxygen, and nutrition are the factors to limit, nutrition being limited in the form of already having been consumed. In a distractionless space they have the room for thought, self reflection, and self evaluation. They come out a bit changed and more mature.

Just going from LC to agar alone will often break a tricky culture. I'd also suggest plating some at the time of bottling. You may see enough of a break at that point to reach your goals without waiting for the culture in cold storage to figure it out. Out of the cold they break more but you don't always need to go that far. These types of methods require some creative thinking and good intuition on behalf of the cultivator but these types of methods also give rise to more opportunity for observation of behavior.

I wish I had better terminology but I don't so I use terms like "meditative state" because that is the most similar term I know of, that commonly I think others can relate to, to describe what I am trying to convey...

Please let me know how this goes for you so I can expand my understanding as well.


--------------------
β€œBetter to be deprived of food for three days, than tea for one.”


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineShroomadillo
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #28591781 - 12/20/23 06:28 PM (1 month, 7 days ago)

These plates were made by taking a single grain from a growth kit and placing it on agar. Is what I see clean mycelium with some metabolites or contamination?




Edited by Shroomadillo (12/20/23 06:28 PM)


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OfflinePnin
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shroomadillo] * 1
    #28591795 - 12/20/23 06:42 PM (1 month, 7 days ago)

If I saw metabolites before a dish was fully colonized I personally would not trust it. What is it supposed to be?


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Edited by Pnin (12/20/23 06:42 PM)


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OfflineGenesisCorruptedS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shroomadillo]
    #28591812 - 12/20/23 06:59 PM (1 month, 7 days ago)

That dark yellow tar looking stuff appears to be bacteria.
Take a look in here about 2/3 down


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Offlinetryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!) [Re: GenesisCorrupted] * 4
    #28591821 - 12/20/23 07:03 PM (1 month, 7 days ago)

I would chuck those plates and find a print


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
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OfflineShroomadillo
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28591977 - 12/20/23 08:53 PM (1 month, 7 days ago)

Alright will toss those plates. In the meanwhile I alrdy cloned the biggest of my fruits, as well as swabbed a plate with some spores. At what point do you suggest making a transfer to another plate?




Edited by Shroomadillo (12/20/23 08:54 PM)


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OfflineNichrome
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shroomadillo]
    #28592148 - 12/21/23 12:30 AM (1 month, 7 days ago)

Quote:

Shroomadillo said:
These plates were made by taking a single grain from a growth kit and placing it on agar. Is what I see clean mycelium with some metabolites or contamination?






wet bubble.


--------------------
β€œBetter to be deprived of food for three days, than tea for one.”


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineBrokenHeart
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nichrome]
    #28594046 - 12/22/23 12:21 PM (1 month, 5 days ago)


APE
I have yet to pour my own.  What you have to do sometimes gets in the way of what you want to.  These are lme agar I bought.  I have 4 others that I used the swab on before depositing it here 5 days ago.  All growth looks more swispy than I expected.  All plates have similar formations.  Is there no way to tell mold from myc at this stage?  The formations look less organized than imagined.


Edited by BrokenHeart (12/22/23 12:23 PM)


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Offlinetryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!) [Re: BrokenHeart] * 1
    #28594099 - 12/22/23 01:18 PM (1 month, 5 days ago)

A big benefit to making your own agar is to be able to dial in nutrient levels and be able to promote different morphology


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
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Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinemistermiyagi
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 2
    #28594125 - 12/22/23 01:44 PM (1 month, 5 days ago)



Other than being a little overgrown, would you take this to grain? It looks kind of uneven to me but several plates from the same variety are pretty similar.


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Offlinetryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mistermiyagi] * 5
    #28594133 - 12/22/23 02:09 PM (1 month, 5 days ago)

It's hard for me to trust an overgrown plate without seeing it prior to being overgrown


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinemistermiyagi
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28594168 - 12/22/23 02:47 PM (1 month, 5 days ago)

F
Quote:

tryptkaloids said:
It's hard for me to trust an overgrown plate without seeing it prior to being overgrown




Fair enough, thanks. I’m going to do another transfer or 2


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InvisibleMRFRY
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Re: AGAR ENVY! (Anything and All things agar!) [Re: mistermiyagi]
    #28594182 - 12/22/23 02:58 PM (1 month, 5 days ago)

Is it worth continuing to transfer these plates? This is my first effort at germinating spores on agar and my first time working with PE. I searched the forums but couldn't find pics/information that would allow me to confidently identify what I have. I'm prepared to be told that I'm clueless and that I've been playing with some other mould.

I streaked two MEA plates with a PE swab and embedded the cotton in a third plate. I saw one site of germination on one of the streaked plates after 2 weeks. It's now 4 weeks and I haven't yet seen any more evidence of germination in any of the 3 plates. I'll try different, softer agar next time.

Plate 1 shows the original site of germination (which was located on streak path). I made 4 transfers with a sterile inoculation loop. The dark speck at the top/top-left in the back-lit pic of plate 1 is white when top-lit. The growth in all plates looks white when lit from above.

Plate 2 is one of the transfers and looks the weirdest with the pronounced spiky growth emanating from the agar chunk in the centre and the trail of mycelium running to the edge of the plate. I can't recall whether I dropped agar from the loop before depositing it in the centre but the underlying agar isn't scraped/dented so I'm guessing not.

Some of the other plates show spiky growth from the transferred agar chunk too. All plates are wrapped with cling film, which I've used in the exact same way with no problem on regular cube plates when working with clones.






Edited by MRFRY (12/22/23 03:26 PM)


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Offlinetryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MRFRY]
    #28594242 - 12/22/23 04:02 PM (1 month, 5 days ago)

7-9 oclock on plate 3 is the most promising


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleMRFRY
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28594359 - 12/22/23 05:50 PM (1 month, 5 days ago)

Thanks, I'll take a transfer or two from there. That section does look the most vigorous. The spiky bits and wispy bits were making me especially suspicious because they didn't look like what I'd seen in my meagre experience with clones on agar.

I'll do more transfers and let the older plates grow out to see what happens.

Just occurred to me that I should have oriented pics of same plates the same way to make pointing out locations easier. I'll do that if I need more help in future


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OfflineRoscoeReturnsS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MRFRY] * 4
    #28597566 - 12/25/23 01:12 PM (1 month, 2 days ago)



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OfflineBajazly
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Re: AGAR ENVY! (Anything and All things agar!) [Re: RoscoeReturns]
    #28597570 - 12/25/23 01:25 PM (1 month, 2 days ago)

Mighty fine lookin there Roscoe, wish I could get anything close to looking like this.


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OfflineSupaThaRipper
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bajazly]
    #28597572 - 12/25/23 01:28 PM (1 month, 2 days ago)

A few things you could try. Lowers nutrients, adding yeast, and sometimes it just takes a few transfers.


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Invisibleoxo
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Re: AGAR ENVY! (Anything and All things agar!) [Re: RoscoeReturns]
    #28597586 - 12/25/23 01:45 PM (1 month, 2 days ago)

Quote:

RoscoeReturns said:




Is there a significance to the mycelium meeting, or just maximizing petri dish usage?


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