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InvisibleFloret
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Registered: 01/02/23
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: Baba Yaga]
    #28187202 - 02/14/23 02:15 PM (11 months, 6 days ago)

Can't wait to hear how they do after two years. What conditions do you store them in? This is pan cambo? Bispo? Cyan?


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LAGM 2024


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InvisibleBaba Yaga
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: Floret]
    #28187251 - 02/14/23 02:51 PM (11 months, 6 days ago)

It's cambo at room temp.


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InvisibleJHOVA
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: Baba Yaga]
    #28187320 - 02/14/23 03:44 PM (11 months, 6 days ago)

This reminds me i should try this tek out.


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InvisibleBaba Yaga
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: JHOVA]
    #28187370 - 02/14/23 04:36 PM (11 months, 6 days ago)

I used his with the semilanceata jars I got here at the moment as I am very unsure if the cultures I used to inoculate were contaminated with mold or not. It's just hard to tell the way the myc looks and the prints are quite dirty. Having the mycelium colonize the top first kind of separated the different types and mold got through first or different types myc were easier to tell apart.




I was running a few pan jars without the PF sub on top and they were doing fine but I still like this a lot.


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InvisibleFloret
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: Baba Yaga]
    #28187992 - 02/15/23 12:26 AM (11 months, 6 days ago)

You make pans look so easy! So many great teks Baba!

Do you toss the jars that show contam on the pf topping?


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InvisibleBaba Yaga
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: Floret] * 1
    #28188015 - 02/15/23 01:22 AM (11 months, 6 days ago)

Thanks buddy and yes, everything gets tossed of course. You will always fuck up in this hobby regardless how long you are doing it for, the trick is to recognize it and toss things as early as possible.


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Offlinefuktardles
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: Baba Yaga]
    #28578385 - 12/11/23 10:02 AM (1 month, 17 days ago)

* * please forgive replying a slightly older topic * *

first of, thank you Baba for posting this; it looks like it may possibly have some potential for giving a boost to some older spores i have that for some reason they seem to be taking a slower approach to life than when i originally grew them. i am in california and obtaining new cube spores is already quite difficult which is part of the reason why i'm using these older spore prints, the other part is that i really liked the way they turned out the last time i grew them, but now they seem to be taking the scenic route as opposed to getting to their destination in an expedient manner.

i have not yet had the opportunity to grow any Pans yet [even harder to get their spores here in cali, or so it seems from my attempts] which seems to have been the impetus for creating this tek but it looks like it should help me out with my cubesque-dilemna, too. But since i will be trying it on cubes instead of Pans, is there anything different that you might recommend?

one final note: i will to follow your tek as is as it seems it best accomplish what i'm want. But while typing this an idea popped up in my head--in regard to your Pans, by chance did you also happen to try mixing your PF-SUB into the grains (instead of leaving the pf-sub on top) and then inoculating with the LC? I'm wondering if the mycelia would then jump off on all or even half of the bits of vermiculite+ already scattered throughout your grain, thereby eliminating the need to wait a few days for the top growth and also the need to shake it because it would have been pre-shook?? If not, maybe the next time you use PF-TOPPING, maybe you may want to try an extra jar or 2 with PF-TOPPING [premixed] to compare.


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take it easy, peace out mf-ers & balls to the wall!
theo


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InvisibleBaba Yaga
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: fuktardles]
    #28578521 - 12/11/23 12:36 PM (1 month, 16 days ago)

Feel free to experiment. You could certainly give it a try and mix the pf sub with the grain and see how the recovery goes. Apart from speeding things up I just like having it colonize on top as it does act like a petri dish placed on top of the grain. That way I have another opportunity to check the health of the culture and especially molds that snug in during inoculation are easy to spot.


Now you are talking about spores. When you use a print or syringe (maybe an old one?) to inoculate then you will still run a high risk of failure if the spores are contaminated with bacteria. It might help with the initial germination and building strong first growth since bacteria is out run easily by mycelium on PF substrate but once you shake the jar it will disperse everything throughout the jar and will make it possible for bacteria to multiply as well.

If you have spores/a syringe which is problematic then you are better off to go the full PF-TEK rout and obtain a clean culture via sample tissue or by taking transfers from the PF substrate or the very least make some nice clean spore prints once you get fruits to make new syringes which might work better for what you like to use them.


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Offlinefuktardles
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: Baba Yaga]
    #28579850 - 12/12/23 11:14 AM (1 month, 16 days ago)

I was doing spores to agar, then agar to LC, and also agar to grain (oats) and LC to grain  and then finally grain to to substrate. Spore to agar always seems a little slow to me. But now it seems like all the steps after the agar are growing much slower than when i initially grew these.

By the way, these are RustyWhytes. The first time around they all seemed to grow at a moderate to quick speed, plus they were large and also more potent than what I had grown before.

Even though they seemed clean on agar, by the time they finished colonizing the grain jars, many would bacteria out or if they did make it grain the substrate would trich out. But now that i think about it,  my problem may have been from trying to grow in the miserable SoCal heat again. If our fucking governor :boo: hadn't vetoed the psychedelics bill, it probably would be easier to get fresh spores here, and i would move on.


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take it easy, peace out mf-ers & balls to the wall!
theo


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InvisibleBaba Yaga
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Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: fuktardles]
    #28579946 - 12/12/23 12:56 PM (1 month, 15 days ago)

Hey, you are already a member for a few years now and if you manage to make 30 more posts (ideally meaning full ones) then you will get access to the marketplace which might make your life easier.

I regards to your problem I would suggest you make a dedicated post to troubleshoot the problems you are having and reviewing your process. Include a few photos of your cultures and explain your work steps a bit and through this discussion you will most likely find a solution to your situation and will have gathered enough posts fr marketplace access.

I will stay logged out for a while and mostly be absent til the New Year, if you do not want to make your own thread then here are a couple of good places to ask questions.


Recognizing and dealing with contamination

Ask Quick Questions, Get Quick Answers


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Offlinefuktardles
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Registered: 08/21/18
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Loc: Southern California
Last seen: 3 days, 5 hours
Re: PF-TEK-TOPPING: A Workaround For Slow LC Recovery [Re: Baba Yaga]
    #28593959 - 12/22/23 10:59 AM (1 month, 6 days ago)

Thanks Baba. frustration set in and whilst doing some winter cleaning i found a bag with some prints. :grin: so, i tossed what i had and i'm gonna start from scratch, making sure to follow the teks. I appreciate the advice. being autistic gives me plenty of issues, but i will try and keep my head together enough to take pics, if i can manage. but at the least i will keep an eye on technique foremost. and go from there. wonderful holidays and fresh year to you and yourn.


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take it easy, peace out mf-ers & balls to the wall!
theo


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