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ghiajake
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Re: The Official Baeocystis Thread [Re: wavyedge]
#28591568 - 12/20/23 03:50 PM (1 month, 7 days ago) |
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Quote:
wavyedge said: Well in my recent testing, rye grass seed soak agar performs slower for colonization than dilute grain soak agar.

On the left is grain (oats) cook water agar. Right is RGS cook water.
What was your Water:Soak Water ratio for each of those batches? The rye grass one seems quite darker than your oat grain one, implying that it is quite higher in nutrients. If you used the same ratio for both, then I'd have to say the grass seed leached out more nutrient during the soak than the oats did. Even if it's just that the grass seed contains more tannins than the oats, tannins are still a nutrient for basically all woodloving species. If it is a secondary decomposer it won't like the higher nutrient content.
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ghiajake
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Re: The Official Baeocystis Thread [Re: ghiajake]
#28591580 - 12/20/23 04:00 PM (1 month, 7 days ago) |
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Would be interesting to know the pH difference between your two agar types, if it was a tannin issue the rye seed one should have a lower pH than the oat one. Agar and grain pH is an issue that just doesn't get talked about enough when discussing cultures stalling out.
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ghiajake
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Re: The Official Baeocystis Thread [Re: ghiajake]
#28591585 - 12/20/23 04:12 PM (1 month, 7 days ago) |
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Has anyone in the group tried starving the culture? Since it apparently doesn't like high nutrients it would be nice to know the lower limit as well. From what I've seen so far all y'all's myc looks tomatose, finding the lower nutrient limit may cause the myc to flip rhizo.
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ghiajake
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Re: The Official Baeocystis Thread [Re: wavyedge]
#28591593 - 12/20/23 04:20 PM (1 month, 7 days ago) |
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Quote:
wavyedge said:

This may just be a trick of lighting, but does the bottom right plate in your pick have radial leading edges from the two 'noc points where I put the red circles? I've seen that kind of "ghost edge" with Hericium sp. often.
Edited by ghiajake (12/20/23 04:32 PM)
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wavyedge

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Re: The Official Baeocystis Thread [Re: ghiajake]
#28591602 - 12/20/23 04:27 PM (1 month, 7 days ago) |
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Nope, myc extends just a tiny bit off the transfers.
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wavyedge

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Re: The Official Baeocystis Thread [Re: ghiajake]
#28591609 - 12/20/23 04:31 PM (1 month, 7 days ago) |
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Quote:
ghiajake said:
Quote:
wavyedge said: Guerilla grow is planting somewhere not on your property.
Not really. A guerilla grow is just a stealthy grow, whether it's on your property or not. All of my beds are "guerilla" because they are just mulched landscaped beds surrounding my house that would still be mulched if I wasn't growing fungi in them. Not one single person who didn't know they were spawned would suspect they are.
https://en.m.wikipedia.org/wiki/Guerrilla_gardening
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ghiajake
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Re: The Official Baeocystis Thread [Re: wavyedge] 2
#28591711 - 12/20/23 05:27 PM (1 month, 7 days ago) |
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Quote:
wavyedge said:
Quote:
ghiajake said:
Quote:
wavyedge said: Guerilla grow is planting somewhere not on your property.
Not really. A guerilla grow is just a stealthy grow, whether it's on your property or not. All of my beds are "guerilla" because they are just mulched landscaped beds surrounding my house that would still be mulched if I wasn't growing fungi in them. Not one single person who didn't know they were spawned would suspect they are.
https://en.m.wikipedia.org/wiki/Guerrilla_gardening
I guess that depends on whether you consider growing psilocybe mushrooms to be "gardening", which I do not. They are not "food, plants, or flowers" to use your link's definition, and since fungi are more related to us than plants you can't appropriately use plant terms at all for cultivating them.
I get that that is more my own Apsie issue than other people care to get into, so let's just look at the relative definition of the word "guerilla" when used as an adjective. Copied from Cambridge's website:
guerrilla adjective [ before noun ] UK /ɡəˈrɪl.ə/ US /ɡəˈrɪl.ə/ using unusual methods to create or get attention for your ideas, art, products, etc., usually ones that cost little money and involve using public spaces:
Yes, it does mention public spaces. But it does not mention using other people's private property like your wiki definition does. That's just illegal trespassing. Notice it says "usually" (not "always"), but begins by saying "using unusual methods". That's a bit of an oxymoron since if people "usually" use public spaces, then the "unusual method" would be to use your own spaces. Besides, building a mushroom bed that mimics a natural bed somewhere that's not on you property is not "guerilla" because it is the usual method they grow anyways, not the "unusual method".
Technically "guerilla gardening" uses "guerilla" as an adverb since "gardening" is a verb, which it does not have a definition for and is not a correct way to use the adjective. Sure, "guerilla garden", "guerilla grow", and "guerilla warfare" are correct usages since they precede nouns, but it defaults back to the standard definition I listed above when used as an adjective.
Anyways, I'm sure nobody cares about about the correct usage and definitions of terms like I do. The point is that "guerilla" boils down to "unusual methods" no matter how it's used, and I argue that mimicking natural processes in areas that they would normally grow anyways if they were local to your region does not fall under the definitions of "guerilla" or "gardening". It's simply just "wild propagation", technically no different than if an animal (or human) ate a fruit and puked/shat it out in a new area for the spores to germinate and colonize.
Edited by ghiajake (12/20/23 05:41 PM)
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Nichrome
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Re: The Official Baeocystis Thread [Re: ghiajake] 1
#28592144 - 12/21/23 12:24 AM (1 month, 7 days ago) |
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Thank you for the etymology lesson on those terms. I learned some stuff and broadened my understanding of my born language and words I use regularly. I love both the origins of elements of language as well as their evolution through time and into the future. Through our interactions we create an evolving vernacular.
If the term guerrilla grow is used in context to a certain vernacular, and is used properly pertaining to that vernacular, then the usage is valid. It would fly in a modern college paper. So would the term fly if contextualized in the same vernacular. Guerrilla growing is well defined and well used in the modern American counterculture vernacular.
In that sense I have done a lot of guerrilla grows and enjoy it very much. My mulch, public places, natural spaces, etc. I was spreading cyanescens around Olympia Wa parks and trails as far back as 01. I try to bring spawn to the huge city mulch piles every year still. That mulch is eventually sold by the city to bag mulch companies that show up with dozens of semi trucks. Then it gets sold worldwide. Doing my part.
So back to the topic at hand, my brf LC is doing splendidly. It will be ready to fill bank vials and play with grains and substrates soon enough. This Wavy spore culture is the fastest baeo I have seen yet.
In the 20's American jazz vernacular, I'm a viper and I'm going to get busy smokin' tea.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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kirkeng
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Re: The Official Baeocystis Thread [Re: Nichrome] 5
#28592696 - 12/21/23 12:31 PM (1 month, 6 days ago) |
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T1 s are taking well it seems, bottom right is a tad funky comparatively. Agar mix is MYA at like 1.4%, I’m not noticing a major drop off in speed. Definitely slower than say cyans but is growing at about the speed of my caerulipes or papuana.

Been gathering some rotted leaves and wood around the yard to use down the line for tests.
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ghiajake
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Re: The Official Baeocystis Thread [Re: Nichrome]
#28592704 - 12/21/23 12:41 PM (1 month, 6 days ago) |
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Quote:
Nichrome said: Thank you for the etymology lesson on those terms. I learned some stuff and broadened my understanding of my born language and words I use regularly. I love both the origins of elements of language as well as their evolution through time and into the future. Through our interactions we create an evolving vernacular.
If the term guerrilla grow is used in context to a certain vernacular, and is used properly pertaining to that vernacular, then the usage is valid. It would fly in a modern college paper. So would the term fly if contextualized in the same vernacular. Guerrilla growing is well defined and well used in the modern American counterculture vernacular.
In that sense I have done a lot of guerrilla grows and enjoy it very much. My mulch, public places, natural spaces, etc. I was spreading cyanescens around Olympia Wa parks and trails as far back as 01. I try to bring spawn to the huge city mulch piles every year still. That mulch is eventually sold by the city to bag mulch companies that show up with dozens of semi trucks. Then it gets sold worldwide. Doing my part.
So back to the topic at hand, my brf LC is doing splendidly. It will be ready to fill bank vials and play with grains and substrates soon enough. This Wavy spore culture is the fastest baeo I have seen yet.
In the 20's American jazz vernacular, I'm a viper and I'm going to get busy smokin' tea. 
I'd argue that "American counterculture vernacular" is just a fancier PC term for the word "slang", but like I said in my reply I totally understand that's more of me being an Apsie than me expecting others to agree with me.
But as you said, back to the main topic... I mentioned in one of my replies to wavy that it would be interesting to know the pH differences between his two types of agar, since that's a topic not usually discussed when talking about cultures stalling. Since you're having good results with BRF LC, have you thought to check your LC's pH?
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YoshiTrainer
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Re: The Official Baeocystis Thread [Re: ghiajake] 1
#28594624 - 12/22/23 10:57 PM (1 month, 5 days ago) |
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Nice thread, very cool species!
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wavyedge

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Re: The Official Baeocystis Thread [Re: YoshiTrainer] 2
#28597942 - 12/25/23 08:15 PM (1 month, 2 days ago) |
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Having some better progress on rye, this time from LC.
Better colonization of the rye grain than I saw with oats. 7 days growth, there's more than shows in the picture, and I could do much better with LC (I'm a noob in that dept.)
Edited by wavyedge (12/25/23 08:16 PM)
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Fungus Gnat


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Re: The Official Baeocystis Thread [Re: wavyedge] 1
#28599728 - 12/27/23 11:49 AM (1 month, 19 hours ago) |
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Has anyone experimented with different colonization temps? All of my attempts have been growing super slow, but I nocc'd another plate on 12/10, didn't see any growth on 12/21, then left for the holidays and had my house cooler at 58F. Came back and the plate has way more growth than any of my previous agar runs. It seems a little unlikely, but maybe it does a bit better in cooler weather.
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Land Trout
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Re: The Official Baeocystis Thread [Re: Fungus Gnat] 4
#28602748 - 12/29/23 09:30 PM (29 days, 10 hours ago) |
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I’ll try that. I put a makarorae jar in the garage a couple days ago to see if it would make a difference, I’ll put one of my baeo jars out there too.
 This one was knocked up on he 2nd, it was in the fridge for about a week.
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Nichrome
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Re: The Official Baeocystis Thread [Re: ghiajake] 2
#28602758 - 12/29/23 09:48 PM (29 days, 9 hours ago) |
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Looks like my BRF and well water LC brine is right about neutral out of the pc and cooled to room temperature.
The Baeo LC is now mature and grew about as fast as a cube LC in the same recipe made the same day. Lightning speed.
It will go to rye very soon.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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ghiajake
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Re: The Official Baeocystis Thread [Re: Nichrome] 1
#28603005 - 12/30/23 06:52 AM (29 days, 51 minutes ago) |
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Quote:
Nichrome said:
 
Looks like my BRF and well water LC brine is right about neutral out of the pc and cooled to room temperature.
The Baeo LC is now mature and grew about as fast as a cube LC in the same recipe made the same day. Lightning speed.
It will go to rye very soon.
Right on, thanks for checking.
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alaskappalachian
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Re: The Official Baeocystis Thread [Re: ghiajake] 4
#28615468 - 01/09/24 04:32 PM (18 days, 15 hours ago) |
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LC #1 kicking off quicker than I thought. (As fast as my melmac and yeti from the same session 3 days ago-ish). Shitty broth too (only had brown sugar (-w- inverse sugar)) and a pinch of yeast, so I was glad to see it do something. Making some brf lcs tonight for more baeo xfers, and I suspect they'll move almost twice as fast on it and colonize way thicker in the end. Made new agar with 50:50 WBR boil broth:H20 to thicken up these cultures too
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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Nichrome
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I put some on grain and it is moving insanely slow and thin, almost invisible but it is moving.
I have 4 jars I noc'd with wild caught axenic Pseudomonas fluorescns bacteria lc. I snatched the pseudomonas off a Cordyceps militaris fruit I found in July in NH last year. I'll let those go a while then add the Baeo lc. Also going to noc another 4 with microscopic slime mold I pulled off my kiwi vines. I equipped the culture in it's amoeba stage with the same P fluorescens culture and now several generations in when the spores from the slime germinate and are in their amoeba form, the same Pseudomonas is still also present. If you magnify the slime spores enough you can see the little bacteria packets. P fluorescens unlocks iron and does all sorts of other wonderful things. Well see how the baeos like grain with pseudomonas, and grain with pseudomonas equipped slime molds.
This type of slime mold is invisible to the naked eye but is common in decaying plant material such as wood mulch. I often see slime molds in cohabitation with wild psilocybe patches. I've seen them eat psilo patches too like conquering invaders, but typically the big puke looking ones not the little tiny ones. The micro ones seem to get along hence my inspiration for the fucking around.
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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kirkeng
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Re: The Official Baeocystis Thread [Re: Nichrome]
#28635085 - 01/25/24 10:58 PM (2 days, 8 hours ago) |
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I’m looking forward to you finding out! Very cool idea man.
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endemic_entheogens
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Re: The Official Baeocystis Thread [Re: Nichrome]
#28635094 - 01/25/24 11:07 PM (2 days, 8 hours ago) |
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Have you seen any other cultures shown improvement from having another organism thrown in with the grain spawn? Curious because I feel that is why contaminated spawn does better outdoors and why pasteurization is better than sterilization in some cases. Seems like a mountain of a project to actually find what organisms help tho.
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