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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Baeocystis Thread [Re: alaskappalachian] * 2
    #28574247 - 12/08/23 11:15 AM (1 month, 19 days ago)

:thumbup: I think LC is the way to go with these, agar is agonizingly slow.


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Invisiblealaskappalachian
Entitiologist

Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: The Official Baeocystis Thread [Re: wavyedge]
    #28574257 - 12/08/23 11:26 AM (1 month, 19 days ago)

That was my train of thought :noargument:  Thought maybe a handful of 250ml GWLCs >> small jars of compost/chips then to bulk pasteurized sawdust/chips/compost mix... Woodlovers always seem to uniformly cruise on unfiltered GWLC...


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"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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Invisiblekirkeng
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Re: The Official Baeocystis Thread [Re: alaskappalachian] * 2
    #28574265 - 12/08/23 11:28 AM (1 month, 19 days ago)

Good to know, haven’t touched lc in a very long time but going to have to break my streak perhaps. Got germination on my plates a couple days ago!


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OfflineNichrome
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Registered: 12/17/18
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Re: The Official Baeocystis Thread [Re: kirkeng] * 3
    #28574486 - 12/08/23 02:24 PM (1 month, 19 days ago)

Pics hopefully tonight. Transferred pieces of "leather" from the top of an old ms lc (spores courtesy of NothingsChanged :hatsoff:) that's been sitting on the shelf for a couple years. The pieces are just starting to reach out into the agar. Also my Wavey MS is ready for T1's. Considering , as the growth looks clean, making an early lc from the germ plate.


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Baeocystis Thread [Re: wavyedge] * 4
    #28575989 - 12/09/23 03:23 PM (1 month, 18 days ago)

Quote:










After 10 days, these have barely progressed.



Whereas at least twice this much growth happened on dishes of low-nute grain water agar. They were taken at the same time.



I think it's time to start getting creative with substrates.


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Invisiblethe_chosen_one
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Registered: 09/11/06
Posts: 2,882
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Re: The Official Baeocystis Thread [Re: wavyedge] * 2
    #28576009 - 12/09/23 03:41 PM (1 month, 18 days ago)

I don't even know how I ended up here. :facepalm: I'm late. Too tired. It's cold. 
A little over an hour out from yelling at people on the drive home..
:crankeyclaus:


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"Luck favors the observant." - Workman



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OfflineTweeq
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Re: The Official Baeocystis Thread [Re: the_chosen_one] * 1
    #28576015 - 12/09/23 03:52 PM (1 month, 18 days ago)

Hey nice! A baeocystis thread :smile: Good one Nichrome!

No plans for them atm. However..think I might still have some spores from last year or the year before.


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OfflineNichrome
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Registered: 12/17/18
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Re: The Official Baeocystis Thread [Re: Tweeq] * 6
    #28576313 - 12/09/23 07:53 PM (1 month, 18 days ago)

So here's the old lc (note the dust layer :laugh2:) and the resulting transfers. These transfers are reaching out into the agar now and they look good.



Wavey's spore. Did 6 transfers from these as well as a no guts no glory germ plate lc (water and a little brf).


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineNichrome
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Re: The Official Baeocystis Thread [Re: Nichrome] * 2
    #28581493 - 12/13/23 03:59 PM (1 month, 14 days ago)

Wavey's spores BRF LC is taking off pretty fast. By no means fast fast, but fast for the critter. Wavey's spore cultures are overall faster to move and less picky about nutrition than other baeos I've worked with.

Seems like the growth on BRF LC is more robust and faster compared to sugar based LC like LME or BRS. I whipped up some agar and added some BRF and dropped the sugar by 1% and added some extra brewers yeast to the mix and the myc seems to move faster with more robust growth on those plates too vs the 1.5% LPME Y agar I started them out on. I'll be continuing in that direction with the brf. I like what I'm seeing.


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineLand TroutM
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Re: The Official Baeocystis Thread [Re: Nichrome] * 4
    #28583119 - 12/14/23 08:02 PM (1 month, 13 days ago)


That’s about how I remember it. Transfer from a plate that’s maybe two years old.


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Offlineghiajake
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Re: The Official Baeocystis Thread [Re: Land Trout] * 1
    #28586199 - 12/16/23 11:58 PM (1 month, 11 days ago)

How interesting... I just made up an ajar jar to 'noc with some beao spores I got from holo, then I saw this thread today... Turns out the print I have is of the slow weaker strain, so if anyone here's got a print (or partial) of wavy's strain I love to get some "under the scope". I've got an idea to try...


So what's the idea? Grass seed, the wonderful nutrient base who's good name has been unfairly besmirched for all times due to Violet/Anne's nonsense.

On the topic of natural habitat, while I've not hunted them myselves it seems that many of the photos of them show them growing in grassy areas. Y'all who have hunted them can maybe confirm or contradict this? If the general consensus seems to be that they are secondary decomposers, and Workman had success fruiting them on manure-based substrates, it seems to me that it may be either a grass/dung species that evolved to live off partially decomposed forest litter, or more likely a secondary forest litter species that learned to survive off grass-based nutrients due to deforestation (natural or anthropogenic).

It may just be me rambling because I made a big batch of dry-sift hash for the holidays, but these and ovoids seem to be species that are in the process of evolving due to catastrophic changes in climate. I'd blame Meltwater Pulse 1a and b for the evolutionary path of ovoids moving from forest litter to river debris, or another of the many great floods that have washed away great forests multiple times throughout geologic history. But while ovoids are common, beaos are more rare. To me this hints at them being younger in their evolutionary path, or at least slower because they are more secondary than primary.

Anyways, I guess what I'm trying to say is I'd like to try beao culture on grass seed soak water agar, and GS "grains" to test my hypothesis.
:lmafo:


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Baeocystis Thread [Re: ghiajake] * 5
    #28591420 - 12/20/23 01:38 PM (1 month, 7 days ago)

Well in my recent testing, rye grass seed soak agar performs slower for colonization than dilute grain soak agar.



On the left is grain (oats) cook water agar. Right is RGS cook water.

On my substrate tests, some baeo results: this species proves difficult when we ask it to jump from agar. These are all 8 days since transfer:

Straw pellets + coir



Starter mix:



Worm castings:



Other dishes are showing less or no growth.


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Invisibleelasticaltiger
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Re: The Official Baeocystis Thread [Re: wavyedge] * 2
    #28591433 - 12/20/23 01:50 PM (1 month, 7 days ago)

There's a few species on my "have to try in this lifetime" list. These are one of them. Fuck libs, fuck azurs, the Baeos are so rare and elusive that I just have to have this someday.

When I'm in a better position where I have some land I will reach out for spores but as of now there's no way I'd be able to make any meaningful contribution to this effort. Like maybe a guerrilla grow but like, who even does that.

This thread is so cool. Thank you to all who are working on this.


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OnlineSupaThaRipper
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Registered: 09/02/13
Posts: 1,489
Loc: USA
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Re: The Official Baeocystis Thread [Re: elasticaltiger]
    #28591442 - 12/20/23 01:57 PM (1 month, 7 days ago)

What is a guerilla grow? And please don’t tell me “indoors” lol because someone has to crack that case


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Baeocystis Thread [Re: SupaThaRipper]
    #28591448 - 12/20/23 02:03 PM (1 month, 7 days ago)

Guerilla grow is planting somewhere not on your property.


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OnlineSupaThaRipper
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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28591476 - 12/20/23 02:25 PM (1 month, 7 days ago)

Nope. Don’t like that!


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Invisiblethirdeyewild
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Registered: 11/01/13
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Re: The Official Baeocystis Thread [Re: SupaThaRipper]
    #28591487 - 12/20/23 02:34 PM (1 month, 7 days ago)

I've got several guerilla grows in the works. Part of diversifying my strategy.


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Invisiblethe_chosen_one
On the Darkslide
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Registered: 09/11/06
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Re: The Official Baeocystis Thread [Re: thirdeyewild] * 2
    #28591502 - 12/20/23 02:46 PM (1 month, 7 days ago)

Fortunately my whole property is a guerilla grow. :lol: I can barely muster the motivation to pick Pelli's across the street right now.


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"Luck favors the observant." - Workman



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Invisiblealaskappalachian
Entitiologist

Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: The Official Baeocystis Thread [Re: the_chosen_one] * 4
    #28591549 - 12/20/23 03:31 PM (1 month, 7 days ago)

Is smelling like a gorilla while you do cult work the same thing?...  I'm not even guilty of that though.

T2s on my unfiltered brf poorly ground ugly agar twice as fast as on diluted oat soak broth.  Got 2 LCs going atm and like half a dozen T2s on various agar recipes.  Wish I had more small dishes to emulate wavyedge's strategy.  Gonna have to look around.  I'd ordered some of the cups supa started a thread about but they came crushed all to hell so have to get creative.


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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Offlineghiajake
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Registered: 01/10/13
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Re: The Official Baeocystis Thread [Re: wavyedge] * 1
    #28591553 - 12/20/23 03:37 PM (1 month, 7 days ago)

Quote:

wavyedge said:
Guerilla grow is planting somewhere not on your property.




Not really. A guerilla grow is just a stealthy grow, whether it's on your property or not. All of my beds are "guerilla" because they are just mulched landscaped beds surrounding my house that would still be mulched if I wasn't growing fungi in them. Not one single person who didn't know they were spawned would suspect they are.


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