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Amazon Shop: ½ Pint Jars, Coir, HEPA Filter

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OfflineTweexican
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Karo Tek Setup
    #2859007 - 07/05/04 04:07 PM (13 years, 2 months ago)

Any of you experts out there have a good way of setting up your jars when making liquid culture using Karo? So far I've tried 4 times with no avail. I have a makeshift flowhood using a holmes hepa unit and an upside down rubbermaid container so I'm very doubtful the fault lies in my serilization. I'm wondering if maybe I'm setting up the jar lids wrong. Any help would be appreciated.


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Invisiblemuse_sick
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Re: Karo Tek Setup [Re: Tweexican]
    #2859030 - 07/05/04 04:26 PM (13 years, 2 months ago)

i don't use a flowhood
just 1/2 pint jars with a single hole in the middle
stuffed with polyfill
make sure yr lid (not the rim but the actual lid part) is upside down, you don't want to seal it
2 tbs karo/100 mls of water
some ppl throw some broken glass down at the bottom...
pc for 15 min @ 15 psi
inject 2ccs spores
incubate for a few weeks
within a few days you will see a cloud of usable myc forming
within a few weeks it is best to remove it from heat
what seems to be the difficulty you are running into?


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:beakedwhale:


Edited by muse_sick (07/05/04 04:28 PM)


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OfflineTweexican
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Re: Karo Tek Setup [Re: muse_sick]
    #2859047 - 07/05/04 04:33 PM (13 years, 2 months ago)

Just contams mostly. I most recently made the mistake of using tyvek in the lid, that was just a nightmare. And I also think I ended up sealing it. I didn't have the lid upside down. I was too afraid of using polyfil ; thinking that it would be an inevitable contam.


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Invisiblemuse_sick
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Re: Karo Tek Setup [Re: Tweexican]
    #2859054 - 07/05/04 04:38 PM (13 years, 2 months ago)

  polyfill works good
but vacuums suck
:lol:
i think we found yr problem
if you have any more questions feel free to pm me


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:beakedwhale:


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Invisiblechunder
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Re: Karo Tek Setup [Re: Tweexican]
    #2859062 - 07/05/04 04:41 PM (13 years, 2 months ago)

I first steam my karo jars with just a plastic lid with a hole drilled in it screwed on. Cover this with some foil to keep water from getting in the hole and then steam for 20 minutes. Remove, cool to room temp, then open on the oven rack and scrape some spores into it, then place a piece of tin foil over the jar, then screw the lid on over this. Let colonize until you need to draw innoculate and then just poke through the tin foil to draw up the juice. This is what I do and it seems to work pretty well, especially with fresh spores.

Are you getting contams and some mycellium too, or just nothing at all? If you're not getting any growth, try increasing the karo 1 teaspoon per 100 ml of water.


Edited by chunder (07/05/04 04:43 PM)


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OfflineTweexican
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Re: Karo Tek Setup [Re: chunder]
    #2860195 - 07/06/04 01:08 AM (13 years, 2 months ago)

im pretty sure its contamed mycellium

im goin with the polyfil lid tomorrow. thanks guys. i'll keep you updated. I'm working on a 48 yar project. Malabar, EQ, B+, and GT.

I'm growing all of these on golden millet. Half will be spawned to poo and straw, the other half of the jars will be cased to straight coir.

I'm prayin. :wexican:


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Invisiblemuse_sick
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Re: Karo Tek Setup [Re: Tweexican]
    #2860198 - 07/06/04 01:09 AM (13 years, 2 months ago)

good luck man
and like i said if you have any ?s PM me


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:beakedwhale:


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OfflineTweexican
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Re: Karo Tek Setup [Re: muse_sick]
    #2885146 - 07/13/04 09:31 AM (13 years, 2 months ago)

Alright, I think I've finally gotten to the point where mycellium has grown in 3 of my 4 karo jars. Just one question, do any of you ever get small "spots" on the mycellium in the karo solution? I have 2 jars with 2 or 3 tiny red spots on the mycellium, does that mean its contamed?


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OfflineKillBill
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Re: Karo Tek Setup [Re: Tweexican]
    #2925119 - 07/25/04 03:27 AM (13 years, 2 months ago)

My friend has two karo jars with two tsp. of karo in each, mixed well with glass shards. he's seeing Myc now and he has the lids DOWN , but 1/4 inch holes in the top with tyvek filters. Are his Karo jars Doomed to Contamination?


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Amazon Shop: ½ Pint Jars, Coir, HEPA Filter

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