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Offlinecloverbrew
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Registered: 08/12/19
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Re: Making slants with Frank [Re: enzofilo]
    #27028707 - 11/08/20 02:15 PM (3 years, 2 months ago)

Just followed this tek. The centrifuge tubes work awesome, lots off room. Question, does the wood need to be exposed a lot? When I tipped mine, had to run errands, got back and just had a tiny bit of wood poking out of the top of agar. Should I redo with more wood exposed?


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OfflineJohnS0N
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Re: Making slants with Frank [Re: FrankHorrigan]
    #27592581 - 12/24/21 03:01 AM (2 years, 1 month ago)

This is one of those threads that you check back on from time to time. Cheers Frank.


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Offlinespuitzakje
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Re: Making slants with Frank [Re: FrankHorrigan]
    #27631759 - 01/25/22 04:42 AM (2 years, 3 days ago)

I have seen multiple people say to put slants (or petri dishes if you use those for storage) in ziploc bags and then in an insulated lunch box. What is the use of putting them in ziploc bags?


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Offlinenerdintheshell
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Registered: 09/11/21
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Re: Making slants with Frank [Re: spuitzakje]
    #27632915 - 01/25/22 09:30 PM (2 years, 2 days ago)

Works similar to parafilm or cling wrap. Provides ge while keeping contam out and keeping the plate together.


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Offlinespuitzakje
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Re: Making slants with Frank [Re: nerdintheshell]
    #27633505 - 01/26/22 09:34 AM (2 years, 2 days ago)

But we already wrap the top of the tube with parafilm or cling wrap..


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OfflineOldAgarrr
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Registered: 04/09/22
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Re: Making slants with Frank [Re: spuitzakje]
    #27749335 - 04/24/22 09:30 AM (1 year, 9 months ago)

Does anyone else find that some of the slants get a really tiny bit of agar gunk in the lid after PCing them? Is it likely to cause any problems? I'm thinking that the mycelium is never likely to colonise it, and it might start feeding a stray contaminant at some point! Any trick to avoiding it? I am currently sterilising a couple of replacement lids for two of my slants as it makes me nervous.


Edited by OldAgarrr (04/24/22 09:34 AM)


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Offlinec01h
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Registered: 11/24/23
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Re: Making slants with Frank [Re: FrankHorrigan]
    #28584598 - 12/15/23 10:18 PM (1 month, 12 days ago)

I haven't found anywhere else that recommends the wood, not saying I disagree.  I'd like to know more though!

The other often-referenced recipe I see is BAM Media M85: Long-term Preservation Medium but the idea of 30 grams sodium chloride seems really sus.


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Offlinetripdawg420
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Registered: 02/02/09
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Re: Making slants with Frank [Re: c01h]
    #28585094 - 12/16/23 09:18 AM (1 month, 12 days ago)

ive always used wood :shrug: all u need is mea :thumbup: idk wtf  sodium chloride is so i wouldnt fuck w it


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How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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InvisibleStipe-n CapMDiscord
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Registered: 08/04/12
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Re: Making slants with Frank [Re: tripdawg420]
    #28585127 - 12/16/23 09:43 AM (1 month, 12 days ago)

Slants aren't even necessary, you can just add a wedge to sterile distilled water in a centrifuge tube, or some other appropriately sized vessel. Dehydrated grain is also a cool method.

Plates can last over a year in the fridge, and LC broth will keep your culture alive  in the fridge for just as long, if not longer.

Slants seem outdated, imo, but still work. :shrug:


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Offlinec01h
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Registered: 11/24/23
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Re: Making slants with Frank [Re: tripdawg420] * 1
    #28585774 - 12/16/23 06:13 PM (1 month, 11 days ago)

Quote:

tripdawg420 said:
ive always used wood :shrug: all u need is mea :thumbup: idk wtf  sodium chloride is so i wouldnt fuck w it





:trollface:


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Offlinec01h
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I'm a teapot


Registered: 11/24/23
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Re: Making slants with Frank [Re: Stipe-n Cap] * 2
    #28585787 - 12/16/23 06:16 PM (1 month, 11 days ago)

Quote:

Stipe-n Cap said:
Slants aren't even necessary, you can just add a wedge to sterile distilled water in a centrifuge tube, or some other appropriately sized vessel. Dehydrated grain is also a cool method.

Plates can last over a year in the fridge, and LC broth will keep your culture alive  in the fridge for just as long, if not longer.

Slants seem outdated, imo, but still work. :shrug:




what's longevity of wedge in distilled H2O @ appx. 45 °F?  To use the genetics, do you just put the wedge on agar?

this is another example of awesome knowledge I wouldn't have other than participating in forum, thank you Stipe


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InvisibleStipe-n CapMDiscord
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Re: Making slants with Frank [Re: c01h] * 1
    #28585816 - 12/16/23 06:26 PM (1 month, 11 days ago)



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Offlinec01h
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Registered: 11/24/23
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Re: Making slants with Frank [Re: Stipe-n Cap] * 1
    #28585870 - 12/16/23 06:48 PM (1 month, 11 days ago)

Quote:

c01h said:
this is another example of awesome knowledge I wouldn't have other than participating in forum, thank you Stipe




Quote:

Stipe-n Cap said:
Stasis:

https://www.shroomery.org/forums/showflat.php/Number/28161262/fpart/all

Desiccated myciliated grain for storage:

https://www.shroomery.org/forums/showflat.php/Cat/0/Number/6886717/an/0/page/0




i'd never even heard of either technique :bowdown:


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:cookiemonster:


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