Home | Community | Message Board


FreeSpores.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Petri Dish, Rye Grain

Jump to first unread post. Pages: 1
Invisibletrade_om
An Old Omelette

Registered: 10/19/03
Posts: 164
4 year old spores germinate after 2 mothes. * 1
    #2858378 - 07/05/04 11:17 AM (12 years, 10 months ago)

Just to let all you guys know, Mr. Endo has sent me a pic of an agar plate that was inoculated with EQ spores on 25/4/04 (as seen in the pic). He told me these spores were not kept in good conditions occaisoanly reaching quite hot temperatures.
After 2 monthes of inoculation these spores began to grow and allready cloned, according to Mr. Endo...


--------------------
Trading omelettes is fun..... :P


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedebianlinux
Myconerd - DBK
Male User Gallery

Folding@home Statistics
Registered: 12/09/02
Posts: 8,272
Loc: Over There
Last seen: 1 month, 14 days
Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2858559 - 07/05/04 12:59 PM (12 years, 10 months ago)

okay... i'll bite since it's so quiet.

a pressing question comes to mind; by your saying "cloned" am i to infer that what i see on the pictured plate was successfully fruited?


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletrade_om
An Old Omelette

Registered: 10/19/03
Posts: 164
Re: 4 year old spores germinate after 2 mothes. [Re: debianlinux]
    #2858759 - 07/05/04 02:48 PM (12 years, 10 months ago)

in cloned i meant to say isolated from the first generation petri dish...


--------------------
Trading omelettes is fun..... :P


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedebianlinux
Myconerd - DBK
Male User Gallery

Folding@home Statistics
Registered: 12/09/02
Posts: 8,272
Loc: Over There
Last seen: 1 month, 14 days
Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2858801 - 07/05/04 03:08 PM (12 years, 10 months ago)

the long germination period may have been a result of dehydrated spores. while dehydration shouldn't necessarily affect vitality it does introduce a rehydration period.

once visible mycelia was established did it seem to grow out at an acceptable speed or was impairment noticable?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineDERRAYLD
Constructus

Registered: 05/13/02
Posts: 1,712
Loc: South Africa
Last seen: 8 years, 6 months
Re: 4 year old spores germinate after 2 mothes. [Re: debianlinux]
    #2860230 - 07/06/04 01:22 AM (12 years, 10 months ago)

Yes, I have also just germinated some 3 1/2 yr old PR spores.
The germination is no real issue, just taking longer.
It`s the actual rate of mycelial growth that tends to be far slower.
Fruiting the culture will most likely yield a new spore print for future use.


--------------------
Derrayld and out of control


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibletrade_om
An Old Omelette

Registered: 10/19/03
Posts: 164
Re: 4 year old spores germinate after 2 mothes. [Re: DERRAYLD]
    #2861164 - 07/06/04 12:25 PM (12 years, 10 months ago)

OK M.E. told me the OLD EQ mycelium grows at a regular rate now, but, the mycelium fails to give out any rhizomorphic lines, though he is still hoping for it to fruit...


--------------------
Trading omelettes is fun..... :P


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePrankster239
Spore Searcher
Male

Registered: 01/06/04
Posts: 517
Loc: Europe
Last seen: 8 years, 3 months
Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2868380 - 07/08/04 12:02 PM (12 years, 10 months ago)

It is mostly the best to try old spores on liquid media or put them in steril water for a few days.


--------------------
Support www.fsre.nl !!!

I love to grow exotics with spores from Sporeworks



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineKillBill
Quick Learner
Registered: 07/02/04
Posts: 109
Last seen: 12 years, 7 months
Re: 4 year old spores germinate after 2 mothes. [Re: Prankster239]
    #2949983 - 07/31/04 10:20 PM (12 years, 9 months ago)

haha I am currently working with 4 year old spores as a matter of fact, i had two out of four Brf jars showed nothing,and a rye showed nothing, I wonder if they're showed activity in a month or so?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleFucknuckle
Dog Lover

Registered: 04/24/04
Posts: 6,762
Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2952230 - 08/01/04 04:34 PM (12 years, 9 months ago)

A true testament to the hardy nature of the tiny spore.


--------------------
What it is, is what it is my Brother.
It is as it is, so suffer thru it.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 6 years, 13 days
Trusted Cultivator
Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2969743 - 08/05/04 10:14 PM (12 years, 9 months ago)

I've grown out original PFC syringes that were 6+ years old. All germinated in 7-10 days! Damn those are some potent fuckers!!
Dehydration plays a big part in prints and long term storage.




Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefelixhigh
KIA
Male User Gallery

Registered: 06/24/01
Posts: 7,543
Loc: Ly
Last seen: 3 years, 11 months
Re: 4 year old spores germinate after 2 mothes. [Re: hyphae]
    #2973385 - 08/06/04 09:34 PM (12 years, 9 months ago)

there are strains that i only find a print each 4-5 years (what a mess of prints i know) and they start just like any non-fresh print... (sometimes the germ is real quick)
and i've used no fridge, just stored it sealed of light and air... (no vaccum though)...
it works wonders...
btw, classic PFs! :wink:


FH


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 6 years, 13 days
Trusted Cultivator
Re: 4 year old spores germinate after 2 mothes. [Re: felixhigh]
    #2973671 - 08/06/04 10:45 PM (12 years, 9 months ago)

No dehydration after 4-5yrs.? Wow that is amazing! :wink: Ya I don't store any prints in the fridge myself either :smile:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,000
Loc: USA Mountain Northwest
Last seen: 1 month, 26 days
Trusted Cultivator
Re: 4 year old spores germinate after 2 mothes. [Re: trade_om]
    #2979296 - 08/08/04 10:30 PM (12 years, 9 months ago)

This gives me a bit of hope. I recently received some very rare spores that are 20 years old. They've been six weeks on agar and so far. . .nothing. I won't toss the dishes, but just leave them be and hope for the best. I sure would like to see these germinate.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehyphae
born to grow
 User Gallery

Registered: 12/13/02
Posts: 6,228
Loc: the rain forests
Last seen: 6 years, 13 days
Trusted Cultivator
Re: 4 year old spores germinate after 2 mothes. [Re: RogerRabbit]
    #2979327 - 08/08/04 10:41 PM (12 years, 9 months ago)

Maybe placing some sterile water in the dish would help with the rehydration process, I also always break the surface of the agar when germinating spores and find 95% of the time the break is the first place to germinate. :wink: Damn 20yrs. what strain? I have several prints of the original PFC's that would be great if I decide to start them up again :laugh: Lemme know how they go RR and GL :wink:


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblefastfred
Old Hand
Male User Gallery

Folding@home Statistics
Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
Re: 4 year old spores germinate after 2 mothes. [Re: hyphae]
    #2980611 - 08/09/04 08:01 AM (12 years, 9 months ago)

I have had similar experiences also... The spores often need to be embedded in the agar, not just sitting on the surface.

You might try using a spreader to embed them. Or you might try an overlay with soft agar.

-FF


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinedutchie
Smokin Aussie
Registered: 07/27/04
Posts: 1
Last seen: 12 years, 8 months
Re: 4 year old spores germinate after 2 mothes. [Re: fastfred]
    #2984505 - 08/10/04 03:31 AM (12 years, 9 months ago)

After begining my lost hobby once again, I have found that 3 year old mazatapec spores rehydrated and germinated in a week. Alas, The p.tamp spores did not.:( They were both of the same age. Weird. The p.cub print was far thicker tho. Both were started in 2% honey water. P.tamp spores were also wiped on agar but also no growth. Its been two months....


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Agar, Brown Rice Flour, Petri Dish, Rye Grain

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* spore germination...some grains better than others? afoaf 1,313 12 02/22/03 02:33 AM
by Peace_Patrol
* Spores germination Petri plate VS PF cake FreeSporePrints 2,791 16 08/10/08 03:32 PM
by FreeSporePrints
* spore germination soulman1990 1,711 7 01/07/07 02:25 AM
by RogerRabbit
* microwave & cboard spore germination: results comario2 1,689 14 10/24/02 02:55 PM
by comario2
* Curious Quandry re: Spore Germination Heruuka 1,327 6 09/30/02 06:43 AM
by zeronio
* New Spore Germination Tek altarego 3,997 10 10/28/11 12:33 AM
by RogerRabbit
* Inducing Mycorrhizal Spore Germination AdoreChampignons 965 4 02/13/09 04:51 PM
by Alan Rockefeller
* Spore germination Mexicana A Yachaj 1,435 4 05/14/02 11:03 AM
by baraka

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit
2,111 topic views. 0 members, 4 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Mycohaus
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.004 seconds on 16 queries.