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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,655
Re: OctopusDisco's first grow log [Re: OctopusDisco]
    #28612703 - 01/07/24 07:32 AM (21 days, 2 minutes ago)

Quote:

OctopusDisco said:
Quote:

LadysKnight said:
The dark coir color and lack of colonized surface with fruits sings too wet, which is odd for such a small vessel.




I figured it would dry out quickly--being so small and all--so I may have been a little heavy-handed with the misting. Those are technically my first fruits, not including the in vitro pins that formed!

I think the micro container served its purpose in the sense that my idle fingers were focused on the container with the least to lose. My expectations were pretty low regarding yield, especially when considering the amount of substrate. To be fair, I don't think I made any terrible mistakes, but I know a little bit more about how to handle the mini containers from observing the micro container.


Quick update on the "cloning a dried mushroom" project: healthy mycelium growth is now visible on 3 plates! I can't for the life of me remember what the fruits looked like before I put them in the coffee grinder, so it'll be a cool little surprise if I'm able to eventually get some clean cultures.




Congratulations on first fruits, you're winning!

Not sure where the coffee grinder fits in, but cloning from dry is no cakewalk. Well done.


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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
Loc: In a bear hole
Re: OctopusDisco's first grow log [Re: LadysKnight]
    #28613331 - 01/07/24 06:39 PM (20 days, 12 hours ago)

Here's the first "flush" of the micro container! Didn't even register 0.1g on the scale, but what it lacks in size it makes up for in cuteness :smile:



In other news, I birthed, dunked, grated, and spawned the other jars about a week ago. I wanted to see what impact substrate depth has on the fruits, so for each jar I disproportionately distributed the spawn/coco mix to two small containers. I have some painters tape to keep track of which jar spawned what container. The first number of the serial number is the jar, followed by container id, followed by spawn date.



Unfortunately, the containers from jar 4 appear to have some sort of growth that I haven't seen outside of my contaminated plates. To my inexperienced eye, it appears too "stringy" to be cube myc, and looks more like the pin mold that I've seen on some of my plates.

I'm thinking the course of action will be to keep an eye on it. If I confirm a pin mold contamination, I'll just toss the container. I figure if its somewhere, it's likely present everywhere.

Let me know if you suggest otherwise! I already have more that I could ever use in a lifetime with my first harvest :laugh2:, but I wouldn't mind taking steps to save the container if possible :smile:



Edit 24/1/7: Forgot to add some photos


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LAGM 2.024

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Edited by OctopusDisco (01/08/24 12:37 PM)


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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
Loc: In a bear hole
Re: OctopusDisco's first grow log [Re: LadysKnight]
    #28613344 - 01/07/24 06:53 PM (20 days, 12 hours ago)

Quote:

LadysKnight said:
Congratulations on first fruits, you're winning!

Not sure where the coffee grinder fits in, but cloning from dry is no cakewalk. Well done.




Thank you! Just having a lot of fun lol.

I used a coffee grinder to grind up the dried fruits. It didn't get to a powder-like texture, but still contained some chunks that I was able to cut in half to take samples from.


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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
Loc: In a bear hole
Re: OctopusDisco's first grow log [Re: OctopusDisco]
    #28614097 - 01/08/24 01:10 PM (19 days, 18 hours ago)

First real flush is dried! 37g wet, around 2g dry lol (I would estimate the amount of spawn to be around 100-150 ml of colonized BRF/verm cake that was grated and then mixed 1:2 with coco at/a bit under field capacity). There was still a bit of substrate attached to the fruits when I weighed them wet, so it makes sense that dry mass was <10% of the wet mass. Still have a lot of learning to do with respect to harvesting, good thing I should have plenty of practice soon!



Also, I didn't like the way this.... thing was looking at me, so I sprayed it with some H2O2. I took a look at this thread that has pictures of what it shouldn't look like and the growth seems a lot like dactylium. Even if it isn't dactylium, it still looks very different from all the other mycelium I've seen. The thing that has me worried the most isn't necessarily the string-like growth, it's the length to which the strings are growing without branching at all, almost like spider silk threads.



Anyway, the H2O2 deed is done. I figured I'd rather try my hand at fighting a potential contamination than watching one take over a container.

The "cloning a dried fruit" project is going well. Unfortunately, I can't take any good pictures because the sauce containers I bought have lids that are difficult to see through. Disposable petris and 500ml media bottles are now priorities on the shopping list.

Thank you to everyone who shares knowledge on this site. I couldn't tell mycelium from my elbow about two months ago, and now I have much-needed medicine. Love you all :smile:


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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
Loc: In a bear hole
Re: OctopusDisco's first grow log [Re: OctopusDisco]
    #28617018 - 01/10/24 11:16 PM (17 days, 8 hours ago)

Happy day, everyone!

Time for another update! First an overall update, then an update of the BRF cakes I spawned to bulk in mini-containers (smaller than a shoebox), then a PHOTO UPDATE of the "cloning a dried fruit" project (the photos aren't great though because I'm an idiot and bought bad sauce containers.

Overall update

I weighed everything I've harvested so far and it came out to 2.4g dry, giving me 24 1# capsules with ~0.1g each :smile:

The overall plan is to let these containers flush out while cleaning up the cultures from the "clone a dried fruit" project and getting some grain ready.

I have access to a lot of different kinds of grain, but it seems like rye berries are standard operating procedure when available. Please correct me here if I'm wrong!!

The labelling convention in the pictures is the same as listed above: [jar #].[container #] [date spawned]. After these stop fruiting, I'm going to employ a new ID/serial system that will make it easier to track (at least for me).

BRF cakes to bulk

I'll provide a text update where appropriate, then just provide photo updates. Dactylium (or whatever the hell that thing was) is dead for now. I'll let you know if it returns.

A tiny spot on 1.1 started to turn green, so I cut it out and isolated the container from the rest.


2.1 has a weird spot that looks different from the rest of the mycelium. I think it's just condensation that dripped from the lid, but I'll keep an eye on it.



Everything else:




Cloning a dried fruit

To recap this project:
  • I obtained some amount of mushies about 6 months ago and ate a certain amount, but not everything. These were very dry and may have been PE, but I am not confident in that whatsoever. ('fruits A')
  • I obtained a different amount of different mushies about 4 months ago and ate a different amount, but not everything. These were kind of parbaked when it comes to  drying, and I would describe them as having been "spongy". Besides that characteristic, I think they were more grey than brown. ('fruits B')
  • I used an electric coffee grinder to grind these up and pack some capsules, and had some leftover that I put in a mason jar with some dry silica packets about 2 months ago.
  • On 1/1, I took some culture samples in my SAB. I'm fairly confident that I got samples both fruits, because there were some very tough caps (fruits A) and some softer stipe matter (fruits B). I was able to get some samples from clean interiors of both, as well as some dirty-as-shit samples.
  • The dirty-as-shit sampleswere basically just remnants of the pulverized fruits tossed in a janky sauce containers filled with nutrient agar. They all are contaminated.
  • The samples from the clean interiors look to have healthy mycelium growth. I took T1s today and will post photos when there is something interesting :smile:


Here's a bunch of photos:



Apologies for having to endure the pictures of the shitty sauce containers. I am 100% ordering some petris to make cleaning cultures easier, but for the amount of grain I'm going to be using at a time (not more than 1/2 pint), I kind of like the smaller containers for agar to grain. I did a test with brown rice where I inoculated with "soft" vs "hard" agar. I won't be using brown rice in spawn that I care about, but I had some in the closet and got what I needed out of the test: soft agar is a clear winner when it comes to inoculation.

This is all to say that I willl be buying petris as well as some different sauce containers. I did some extensive searching through the Amazon and came across a review that sold me:


Not that I'll need to PC these, but it seems like that reviewer knows what I want to use these for, generally speaking.

That's all for now! I'll probably have another upate this weekend :smile:


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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
Loc: In a bear hole
Re: OctopusDisco's first grow log [Re: OctopusDisco]
    #28618688 - 01/12/24 01:01 PM (15 days, 18 hours ago)

Happy day, Shromies :smile:

Quick update:

Contaminated container 1.1 (green spots)

I mentioned I observed a green spot in container 1.1 in my last post. I did some research on green contaminants and apparently once it's green, it's sporulating (read: "fucked"). Since we're just having fun here. I decided to try to fight the contaminant, cutting out the green portion and isolating the container from the others.

Looked today and I observed some more small green growth:



Seems like I have a handful of options:

1) Burn it all
2) Throw it outside, throw some debris on top, and see if anything happens
3) Fight it

Leaning toward option 1. I have other containers that look clean, so I don't need this one to fruit.

Option 2 sounds like a pretty low-lift effort, but given the amount of substrate and the potential for yield that it implies, any effort might be too much effort.

Option 3 doesn't sound appealing compared to the other options, but I'm open to trying something out if any of you have a strong conviction.


Cloning dried fruit

3 of the 10 plates looked to have some healthy mycelium growth (hoping it's not just mold, but we'll find out), and 1 of those 3 plates seemed more aggressive than the other 2. I took 4 transfers from each of the 3 plates on 240110--all of which are looking good, pictures coming with the next update.

The picture below is of the more aggressive culture and its growth through the openings in the agar that were the result of the transfers.



I'll probably have another update this weekend :smile:


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InvisibleLadysKnight
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Registered: 10/09/15
Posts: 1,655
Re: OctopusDisco's first grow log [Re: OctopusDisco]
    #28618725 - 01/12/24 01:45 PM (15 days, 17 hours ago)

The clone is looking ok as far as I can see. Regarding the contaminated tubs, they all go out to the compost pile when I see mold. Sometimes they recover enough to fruit, sometimes not.


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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
Loc: In a bear hole
Re: OctopusDisco's first grow log [Re: LadysKnight]
    #28618759 - 01/12/24 02:18 PM (15 days, 17 hours ago)

Quote:

LadysKnight said:
The clone is looking ok as far as I can see. Regarding the contaminated tubs, they all go out to the compost pile when I see mold. Sometimes they recover enough to fruit, sometimes not.




Thanks for checking in, LK! I'm relieved to hear you say that the clone culture looks ok. I just ordered some 60mm petris and they should arrive this weekend, so I'll be able to post some better pictures of these clone cultures in about a week or so. In the meantime, thank you for putting up with these difficult photos.

I have vermicompost going right now, so it looks like this sub is going to become worm food :smile:


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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
Loc: In a bear hole
Re: OctopusDisco's first grow log [Re: OctopusDisco]
    #28628338 - 01/20/24 09:35 AM (7 days, 21 hours ago)

Happy day, everyone!

I feel like a lot has happened since the last post, so let's get caught up :smile: Overall, the strategy is to:

1) Fruit everything that is currently in bulk or grain (just started a few more grain jars, more detail below)
2) Pick 1 unique phenotype characteristic from each of the 3 genetic sources I have and selectively breed until the characteristic appears consistently from spore
3) Do some crosses using various methods

BRF Cakes to Bulk

I ended up tossing the container that had signs of mold. Figured the worms would get more utility out of it than I.

The sub that put out a first flush already put out a whopping 3 fruits on the second flush. It had some questionable spawn to begin with, so that one will be going to the worms as well!

There is some id info on painters tape. The first number represents the jar the spawn came from. The second number is the container id (not important at all). The date is the date spawned to bulk.

So pumped to see some fruits in the containers with larger subs! Hopefully these will be a bit larger than the fruits from the other sub. I think there's something going on with 2.1, but at least we're getting some fruits!



Cloning a Dried Fruit
This project is going great! 3 of the samples ended up producing mycelium. I took some T1s a while back (2 to normal agar, 2 to soft agar) and let those grow out. While all 3 look clean, one of the cultures is different from anything I've seen yet, and the words that keep coming to mind are "vigorous" and "thicc" (with two "c's"). Within a couple of days, the mycelium had pretty much filled in  the gas in the agar where I took the transfers. Trying to manage expectations, but I'm excited to see what kind of fruiting bodies this culture produces. I took a piece of this culture to water agar, and immediately it sent out a mycelium "rope" (12 o'clock in the picture), where the other two did not.



The other 2 cultures look great, but more similar to the Golden Teacher mycelium I've been working with.



After the cultures grew out on the soft agar, I tossed the best-looking plate of each into it's own jar of rye berries. I feel the rye is too dry, so I'll cook it longer next time.

These jars use a new serial id system to help me keep track of cultures (info linked in my signature). The single digits all by themselves have no meaning (I wasn't able to remove the  permanent marker from the last run).


Unsurprisingly, the culture that was most vigorous on agar is most vigorous in the jars (2.1.240117)



Whole Brown Rice Grain Spawn

I think this is the first time I'm mentioning this project. I wanted to experiment with normal vs soft agar, so I  put some spores to agar, transferred once to their respective hardness agar, then went agar to brown rice. I sincerely apologize for neglecting my duties and failing to document the process with photos. The learning here is that soft agar was much faster at colonizing the brown rice compared to the normal agar.

I'm also curious about how "fruitable surface area" to sub volume ratios will impact fruiting characteristics. This is in no way a scientific study; observation is an integral part of the scientific method, however. The purpose of this study is to:

1) Observe any differences in fruiting characteristics that can be used to motivate an actual experiment
2) Test the performance of these new deli containers and compare them to the containers that are fruiting currently (purple and blue lids).

The goal is to dial in a process that I  can use to run scientific experiments with a decent sample size of around 60 subjects per experiment (30 test, 30 control). The deli containers would be great because it allows for a greater degree of control. I just need to make sure that the sub volume is enough to produce fruits that would be expected in larger monos.



Thanks for reading!


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InvisibleOctopusDisco
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Registered: 12/10/23
Posts: 51
Loc: In a bear hole
Re: OctopusDisco's first grow log [Re: OctopusDisco]
    #28632668 - 01/23/24 09:41 PM (4 days, 9 hours ago)

I have an update, but not as big of an update as I would like.

This isn't self-deprecating play on words about the harvest. I'm completely satisfied with the haul, it's just that I took a bunch of photos this morning of the containers I was going to harvest, as well as some agar work, but I didn't realize until I went to take the pictures below that there was no card in the camera :facepalm3: I was too disheartened to take the agar photos again, but I'll take some more in a day or two.

Luckily, there will be plenty more photo opportunities!

BRF Cakes to Bulk

Harvested containers 1.2 and 2.1 (some photos of these in posts above), yielding about 7 and 6 grams, respectively. I'm definitely learning more and more with each flush. Regarding how much FAE to provide, I really like the containers I'm using. They have 4 latches, and when all 4 are engaged, there is a pretty airtight seal; the more latches that are disengaged, the more FAE is provided. I never had all 4 latches engaged at the same time.

I did a lot wrong with the first container that I harvested on 240108 (container 3.1). The main thing I did wrong was that I actually tried to do things to it after spawning. Granted, the cleanliness of the spawn was suspect, but I opened it a lot and misted it from like day 2. Comparing the progress of 3.1 to the two I just harvested (especially 1.2), this is now my mantra:



For container 1.2, I had three latches engaged AT ALL TIMES, never opening to fan, mist, take photos, peek in, breathe on, etc.

Once the mycelium popped through the top layer, surface conditions stayed real nice, knots formed, followed by pins. The most recent harvests had more well-defined clusters as opposed to an even pin set, but I don't think I can point to any reason why this is the case.
Edit 240124: After pins were formed and fruiting was underway, I only kept two latches engaged. I disengaged all latches toward the end of the flush.

I'm still working on getting my harvesting technique dialed, but it, too, gets better with each harvest. I had originally planned to chop and stump, but instead I pulled out the clusters and tried to get as much of the substrate off as I could, then dried in an oven on low with the door open.

I ended up cloning the fruit from container 2.1 that is in the top-left corner, as it is the largest fruit I've gotten to-date (a whopping 4 grams wet).

Container 1.2 (7 grams dry)


Container 2.1 (6 grams dry)


I'll take some photos of everything else give an update soon!


Edited by OctopusDisco (01/24/24 11:50 AM)


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