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OfflineNichrome
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Registered: 12/17/18
Posts: 6,486
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Re: The Official Baeocystis Thread [Re: wavyedge] * 2
    #28562054 - 11/30/23 11:49 AM (1 month, 27 days ago)

Quote:

wavyedge said:
Aww shit! I'm in, for sure! :rockon:

This was my find in Oct, I took clones and spores that many of you now have:





Clones:



Temps around the start of fruiting:

(Scroll through Oct, pics are Oct 23)

https://www.timeanddate.com/weather/canada/burnaby/historic?month=9&year=2023






Mine is moving across MEA right now. Germinated in just a few short days. Thanks Wavy!

I have another LC that has sat on the shelf for the last 3 or 4 years with a very nice healthy white leather on top. I'll pull a little piece out and plate that up too. Those spores were collected in Bellingham, WA and the LC is an MS. The furthest I got with that culture was fully colonized grain. I wasn't able to get the grain to take to any kind of substrate though I didn't try a lot of things.

This is quickly getting more interesting.


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Baeocystis Thread [Re: Nichrome] * 5
    #28562202 - 11/30/23 01:54 PM (1 month, 27 days ago)

I sat down and made some transfers from the clone plates today. I have been trying out these takeout dishes for agar plates that maybe have less waste plastic, and are definitely cheaper than the 100mm celltreat dishes I typically use:

https://www.amazon.ca/gp/aw/d/B07TK3NBLQ?ref=ppx_pt2_mob_b_prod_image



Thanks to @SupaThaRipper for that idea, seem to be working out real well, even better for experimenting with different substrates.

When I collected the specimens, I also collected about 1/2 L of the original sub, hoping to get it to grow at home. That turned into a gnat infested mess in the fridge, so I PC sterilized it in a ziplock round. I also made up ziplocks of hardwood sawdust and some pine shavings/bark.

I put some of those substrates into takeout plates and transferred from the "polar bear pubes" dish:





Edited by wavyedge (11/30/23 04:11 PM)


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OfflineLand TroutM
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Re: The Official Baeocystis Thread [Re: wavyedge] * 1
    #28562214 - 11/30/23 02:06 PM (1 month, 27 days ago)

That’s a pretty neat way of doing it, could probably vac seal them with a weight in the vac bag and drop that in a hot water bath for pasteurizing. I like the space you’re saving while doing several tests. That’s where I burn myself out, doing too many things over too much space and material.


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Baeocystis Thread [Re: Land Trout]
    #28562226 - 11/30/23 02:15 PM (1 month, 27 days ago)

One really nice part of those containers is they are sold as "leak proof" so I don't need to wrap the plate. That's what sold me on them.

Edit: sorry if I wasn't clear but these cups are disposable, polyethylene and come sterile in practice. I don't think they'd survive even pasteurization temps.

I sterilize the sub and used a sterile implement to move some into each cup. It does make me wonder if I could try to fruit directly from the cups...


Edited by wavyedge (11/30/23 03:34 PM)


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OfflineNichrome
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Registered: 12/17/18
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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28562566 - 11/30/23 05:56 PM (1 month, 27 days ago)

If somebody digs up and posts info on the phylogeny and any sequencing info, I'll tack it into the OP.


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Baeocystis Thread [Re: Nichrome] * 1
    #28562584 - 11/30/23 06:02 PM (1 month, 27 days ago)

Quote:

Nichrome said:
If somebody digs up and posts info on the phylogeny and any sequencing info, I'll tack it into the OP.




This feels like a relevant iNaturalist observation:

https://www.inaturalist.org/observations/5700142


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OfflineViridis420
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Re: The Official Baeocystis Thread [Re: wavyedge] * 4
    #28563986 - 12/01/23 04:15 PM (1 month, 26 days ago)

Sweet thread count me in!

Neosporen perhaps he'll chime in was successful growing baeos. Hit shaved maple tree wood with LC I believe.

I've been playing around with these of late using WBS for spawn. I had a couple stumps get ground down and removed. These shredded doug fir stumps mixed with Oregon ground clay soil is what I've been experimenting around with. I sterilized some of that and have a culture growing in 1/2 gallon sized jars. We'll see how that responds.

Also going to see about making planters of that mix with colonized spawn buried inside.

This particular baeos culture I'm playing with I got from a member out of Nova Scotia. Has these growing naturally in his yard lucky bastard around yard shrubs with wood chips spread about.

Here is a pix of how mine looks on WBS.


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Baeocystis Thread [Re: Viridis420]
    #28564002 - 12/01/23 04:31 PM (1 month, 26 days ago)

Sweet! Looks really good. How long did it take to colonize that wbs? Did you use lc?


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Invisibleelasticaltiger
Like Tigers in Coitus
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Registered: 06/24/13
Posts: 8,042
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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28564015 - 12/01/23 04:40 PM (1 month, 26 days ago)

:threadmonitor:


--------------------
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EZEKIEL 23:20


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OfflineLand TroutM
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Re: The Official Baeocystis Thread [Re: Viridis420]
    #28564172 - 12/01/23 06:15 PM (1 month, 26 days ago)

Quote:

Viridis420 said:


Here is a pix of how mine looks on WBS.




That looks amazing compared to how mine looked.
I believe they were first described in Eugene Oregon.


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InvisibleHILLBILLY OUTLAWS
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Registered: 04/21/22
Posts: 2,852
Loc: Luckenbach Texas
Re: The Official Baeocystis Thread [Re: Nichrome]
    #28564477 - 12/01/23 09:24 PM (1 month, 26 days ago)

Good stuff. Can’t wait to see what everybody contributes to the thread. I’ll be lurking and watching!


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InvisiblerhizoRider
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Registered: 12/24/13
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Re: The Official Baeocystis Thread [Re: HILLBILLY OUTLAW]
    #28564967 - 12/02/23 08:53 AM (1 month, 25 days ago)

:congrats:wow :popcorn:


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Invisiblethirdeyewild
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Re: The Official Baeocystis Thread [Re: Nichrome] * 3
    #28565157 - 12/02/23 11:42 AM (1 month, 25 days ago)

Broke open a punky Douglas fir and harvested it for substrate. Nice and clean, no dirt or needles. The big chunks crumble easily.

Those are large ziplocks


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Offlinehuicholstudent
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Re: The Official Baeocystis Thread [Re: thirdeyewild]
    #28565705 - 12/02/23 06:21 PM (1 month, 25 days ago)

Ok I just put some oldish spores to agar we’ll see what happens.

Thanks everyone for sharing their secondary decomposition substrate ideas. If my spores germinate I’ll probably try an old Fir stump as well since I have one in my yard.

For folks who have recently put spores to agar, how long is it taking to see growth on your plates?

Thanks everyone


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Invisiblethirdeyewild
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Re: The Official Baeocystis Thread [Re: huicholstudent]
    #28565719 - 12/02/23 06:27 PM (1 month, 25 days ago)

3 days. Mine are super fresh.


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Offlinehuicholstudent
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Re: The Official Baeocystis Thread [Re: thirdeyewild]
    #28565728 - 12/02/23 06:34 PM (1 month, 25 days ago)

Wow that’s quick! My spores are a few years old so I doubt they’ll germinate that rapidly. Looking forward to see how it goes once you inoculate the fir.


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Invisiblethirdeyewild
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Re: The Official Baeocystis Thread [Re: huicholstudent]
    #28565747 - 12/02/23 06:53 PM (1 month, 25 days ago)

Me to, if the mycelium likes it I'll go back to the tree, pulverize a lot of it and bury some spawn.


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Invisiblewavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
Re: The Official Baeocystis Thread [Re: thirdeyewild] * 6
    #28566155 - 12/03/23 12:35 AM (1 month, 25 days ago)

Came back to life after the transfer, but will it leap?

Sawdust


Original substrate of find


Pine shavings and bark


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InvisiblerhizoRider
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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28566174 - 12/03/23 01:47 AM (1 month, 25 days ago)

Nice I think sawdust looks good 👍 👌 :awedance::congrats:


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Invisiblealaskappalachian
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Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: The Official Baeocystis Thread [Re: rhizoRider] * 3
    #28574222 - 12/08/23 11:01 AM (1 month, 19 days ago)


Fun transfers to make this morning. These spores germed pretty fast for the temperature I'm keeping the room they're in.  Probably end up making Josex-poke LCs out of some T2s if these grow out clean enough. :skatebart:


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"First we build the tools, then they build us."

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