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OnlineLand TroutM
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Psilocybe makarorae cultivation * 26
    #28563995 - 12/01/23 04:22 PM (1 month, 26 days ago)

Psilocybe makarorae


Psilocybe makarorae is related to subaeruginosa/cyanescens/allenii/azurescens but is currently on its own branch of the tree. It occurs naturally in New Zeeland on wood, specifically fruiting from fallen dead branches of southern beech trees.  Thank you to TrustMycelium for sharing prints with others and I from fruits they had found. Now we can all have a chance to learn a little more, and hopefully multiply this shy little organism to make a mycelial blanket to cover the land mass in the northern latitudes. Seeing that is occurs on dead fallen branches I hope to find it to have the ability to occur in more natural settings across a wider habitat Vs. the subaeruginosa types which for the most part are dependent on human activity, like chipping and landscaping.
P. makarorae iNaturalist

Its worth taking note that there was some recent confusion between Psilocybe makarorae and angulospora. Many of the photos that pop up when you google, and even the photos on this shroomery makarorae page are pics of angulospora. Psilocybe angulospora is in a different section of the phylogenetic tree. here is Inski's angulospora grow, which at the time it looks like it was believed to be makaroae. I am almost certain now that because I wrote that down when I send tissue of these to get sequenced Ill have to edit makaroae out of this post, cause the ITS isn't going to match.
:retrocool:

As far as I know everything matches up
I didnt take the best notes this first time around, I didnt take very good pics until later, but i will try my best to share the hard details, important or not, with you all. The biggest reason I had not taken good pics earlier is because the mycelium on agar, grain, and wood substrates in vitro is not very photogenic.  It is slow in vitro, These details sound universal with the other people who got prints, and cultivated them. Ill take notes this time around so that we have them.

Inoculation on 5/25/22, pic taken June 11th, If I remember right the print was sparse, so it looks like very little germination points. But it only takes a little.


A T3 plate transfered to on 11/13/22. pic taken 8/1/23. Ive never seen another wood lover look anything like that. Note that i was only 3 transfers away from inoculation day, May 25th. 


A pic of it in the ground on 2/4/23. The whole reason I don't have any good visuals to share of this in vitro is because it was never photogenic. It was just slow, and weak looking. I did more tossing substrates out because I had to evaluate what I was going to focus on, vigorous productive fungi, my family, or this slow weak looking thing with no path to follow to cultivate it. You can see why it took a back seat. I did more tossing here and there just to make room for other stuff. But then here in the ground it looks very healthy, and its like the cyanecsens subaeruginosa types, but it still has its own look. This is either a bag or shoebox of alder pellets and potting soil that was inoculated with grain, just stomped into a little trench with some chip pile chips tossed on top.

The hanging basket would have been made up of woodchips, and potting soil, and I don't remember if I spawned it with grain, or with a block or shoebox of alder pellet and potting soil, but thats the base. I must have made it late summer, or fall 2022. Pins were observed 11/15/23



Some kind of Coprinus type of thing growing with makarorae

The most mature fruits lost the damp look after being frozen, I know there's a better word for the characteristic.

For the most part i just spawned the hanging basket and walked away for 12 months. I looked at it once and awhile and it was alive. I left it hang outside all winter and summer. From freezing in the cold, to the heat and sun of the summer. Our winters are cold and wet, it gets into freezing but rarely stays frozen all day. Summer conditions are hot and very dry, days were reaching over 105f/40c. I let it stay relatively dry through the summer. Once it started getting cooler I set it on the ground and started watering it around the end of September, early October, just around the time of year a jacket makes it a lot nicer to be outside, and you start thinking about firewood, or propane or whatever you heat your home with. It would get a lot of worms and roley polies, which for some reason bothered me, so I started hanging in in the greenhouse where it stays pretty humid and cool. Temps had been been steady between 32-60f/0-15c for many weeks. The first pins showed on the side that was facing south, it is a little shaded from an oak tree to the south and the panels are shaded and dirty so it wasn't blaring sun, but it seems significant, id think the temp would fluctuate more on that side. We had temps drop to 20f/-6c and I let it freeze two nights in a row. The fruits froze solid and thawed back out. Yeah, I was scared as shit that it would kill them and turn them to mush, but I really wanted to find data on their tolerance. I ran a heater after that as I would have felt terrible it Id lost the opportunity to share with others just to know how long they can get frozen for before they die. They can freeze just fine, I doubt it does them any good. They slowed to a stand still during the cold snap that was about a week. The first day above freezing there was noticeable growth.
This is still a work in progress, i did look at a gill fragment under the scope and did see spores, but I feel there is more maturing for them as of today, 12/1/23.


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Offlinetryptkaloids
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Re: Psilocybe makarorae cultivation [Re: Land Trout]
    #28564012 - 12/01/23 04:38 PM (1 month, 26 days ago)

:popcorn:


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Invisiblethirdeyewild
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Re: Psilocybe makarorae cultivation [Re: tryptkaloids]
    #28564023 - 12/01/23 04:43 PM (1 month, 26 days ago)

:popcorn:


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OfflineJW123
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Re: Psilocybe makarorae cultivation [Re: thirdeyewild] * 1
    #28564089 - 12/01/23 05:35 PM (1 month, 26 days ago)

Great post! You kill it with these woodlovers.
:camping:


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OnlineLand TroutM
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Re: Psilocybe makarorae cultivation [Re: JW123] * 1
    #28564102 - 12/01/23 05:38 PM (1 month, 26 days ago)

They kill me too:loveheart:
Went through a lot of plates in storage a little bit ago and came across some t2s and germ plates. Pics coming soon.


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InvisibleBaba Yaga
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Re: Psilocybe makarorae cultivation [Re: Land Trout]
    #28564110 - 12/01/23 05:47 PM (1 month, 26 days ago)

Awesome work LT :rockon:


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Invisibleelasticaltiger
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Re: Psilocybe makarorae cultivation [Re: Baba Yaga] * 1
    #28564137 - 12/01/23 05:59 PM (1 month, 26 days ago)

So fucking cool. Thank you!


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InvisibleStipe-n CapMDiscord
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Re: Psilocybe makarorae cultivation [Re: elasticaltiger] * 1
    #28564139 - 12/01/23 05:59 PM (1 month, 26 days ago)

Great post, trout:rockon:


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OfflinePnin
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Re: Psilocybe makarorae cultivation [Re: Stipe-n Cap] * 1
    #28564145 - 12/01/23 06:00 PM (1 month, 26 days ago)

:wobble:

They're so beautiful!


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OnlineLand TroutM
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Re: Psilocybe makarorae cultivation [Re: Baba Yaga] * 6
    #28564148 - 12/01/23 06:02 PM (1 month, 26 days ago)

Some germ plates from 5/25/2022



Some of the T2s

These pics are from today, the myc starts out a lot like pan, or semilanceata myc, then forms those veins as it matures.


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InvisibleinskiM
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Re: Psilocybe makarorae cultivation [Re: Land Trout] * 5
    #28564188 - 12/01/23 06:28 PM (1 month, 26 days ago)

Nice work Land Trout.
I recently revived some cultures of this species from slants that were in the fridge for six years, three out of the four isolates are still viable and are now looking really good in liquid cultures.

I wonder how your phenotype compares to the macroscopic characteristics of the wild collection your genetics came from originally, they look a bit different to my collections.

This is one of the wild collections I made for comparison.


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OnlineLand TroutM
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Re: Psilocybe makarorae cultivation [Re: inski]
    #28564204 - 12/01/23 06:38 PM (1 month, 26 days ago)

Thank you Inski, I half thought of asking you to do some wditing for this post, but I figured I would simplify it for now. It would be neat to compare, such a cool mushroom tucked away in a small part of the planet. Did I communicate the confusion between makarorae and angulospora accurately and clearly? Hopefully trustmycelium logs back on when your season comes around down there. I’m pretty sure these came from Dunedin.


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Invisiblewavyedge

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Re: Psilocybe makarorae cultivation [Re: Land Trout]
    #28564349 - 12/01/23 07:49 PM (1 month, 26 days ago)

:rockon:


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OfflineYoshiTrainer
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Re: Psilocybe makarorae cultivation [Re: Land Trout]
    #28564352 - 12/01/23 07:51 PM (1 month, 26 days ago)

Great job LT, those are beautiful!


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Invisiblethe_chosen_one
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Re: Psilocybe makarorae cultivation [Re: YoshiTrainer]
    #28564409 - 12/01/23 08:32 PM (1 month, 26 days ago)

:manofapproval:

Yeah this is pretty damn cool. Nicely done!

Nice to see an inski post too. I remember you used to tell me I should post more. Seems kind of the other way now. :lol:


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OfflinePluviophile
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Re: Psilocybe makarorae cultivation [Re: Land Trout]
    #28564431 - 12/01/23 08:43 PM (1 month, 26 days ago)

:kaneclap:


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InvisibleMr Piggy
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Re: Psilocybe makarorae cultivation [Re: Pluviophile]
    #28564633 - 12/02/23 12:40 AM (1 month, 25 days ago)

:datass:


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OfflineDERRAYLD
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Re: Psilocybe makarorae cultivation [Re: Mr Piggy]
    #28564688 - 12/02/23 02:36 AM (1 month, 25 days ago)

:wtf3:

Amazing dude


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OfflineScrewup
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Re: Psilocybe makarorae cultivation [Re: DERRAYLD] * 1
    #28564690 - 12/02/23 02:51 AM (1 month, 25 days ago)

Do they get you high?? Is it different from cubes?


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Invisiblealaskappalachian
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Re: Psilocybe makarorae cultivation [Re: DERRAYLD]
    #28564693 - 12/02/23 02:52 AM (1 month, 25 days ago)

:vibin:
That one T3 looked wild AF


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