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OfflineNichrome
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The Official Baeocystis Thread * 13
    #28561041 - 11/29/23 04:57 PM (1 month, 28 days ago)

P. baeocystis is definitely one of the more fascinating species of active mushrooms. I fell in love with them decades ago when I would find them amongst other woodlovers in the fall. They were always so mysterious in their nature and the way they conduct themselves. Upon eating them for the first time I realized just how special they really are.

This species has largely escaped the modern mushroom boom. Something in their elusive nature that carries through I guess. Not only do they evade our attention but those of us who have tried working with them have found it increasingly more mysterious and downright difficult. You can't spell difficult without cult.

This is a thread dedicated to the lovely Psilocybe Baeocystis in all it's glory. Let's work together to open the box on this species and let it's secrets out. It is a strong one, and I think right now there is enough community gusto to crack the codes and let loose on the world what this beautiful species has to teach us.


(I do plan to edit the op with more info and links and such)


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Invisiblethirdeyewild
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Re: The Official Baeocystis Thread [Re: Nichrome] * 3
    #28561056 - 11/29/23 05:08 PM (1 month, 28 days ago)

Good call! I'm in. About a week in from germinating my first baeo spores and I'm thrilled to be able to work with these. Never tried them but spent many fruitless hours looking for them.
Hope to be able to pass some spores along one day.

Let's hope we can crack these like the community has so many other exotics.


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Edited by thirdeyewild (11/29/23 05:11 PM)


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Invisiblealaskappalachian
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Re: The Official Baeocystis Thread [Re: thirdeyewild] * 3
    #28561064 - 11/29/23 05:12 PM (1 month, 28 days ago)

:radman:  Wonderful idea.  Streaked my plates this morning.  It was the photo of these in the Audubon Field Guide I got when I was 12 that inspired my interest in psilocybe species, and being able to attempt cultivating these is a dream come true.  Can't wait to see what is contributed here...


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Invisiblethirdeyewild
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Re: The Official Baeocystis Thread [Re: alaskappalachian] * 3
    #28561073 - 11/29/23 05:19 PM (1 month, 28 days ago)

The cursed text! :rofl:
I was also inspired and misguided by the Audubon. Wanted baeos and trudged though bogs looking for the 'bog conocybe'

So awesome to have accessed both through the community!


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Edited by thirdeyewild (11/29/23 05:22 PM)


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Invisiblekirkeng
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Re: The Official Baeocystis Thread [Re: thirdeyewild] * 3
    #28561093 - 11/29/23 05:39 PM (1 month, 28 days ago)

Guess I better get around to streaking some plates…


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OfflineLand TroutM
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Re: The Official Baeocystis Thread [Re: kirkeng] * 3
    #28561105 - 11/29/23 05:47 PM (1 month, 28 days ago)

Ohhhh shit, now that there’s a thread I’ll have to pull out a culture


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OfflineJW123
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Re: The Official Baeocystis Thread [Re: Land Trout]
    #28561235 - 11/29/23 07:20 PM (1 month, 27 days ago)

:thisisgonnabegood:


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Offlinehuicholstudent
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Re: The Official Baeocystis Thread [Re: JW123]
    #28561382 - 11/29/23 09:03 PM (1 month, 27 days ago)

This is great—thanks for creating this thread. I’ll dust off some old spores and see what happens too. I’d love to learn from anyone having success with these elusive teachers.


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OfflineFungus Gnat
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Re: The Official Baeocystis Thread [Re: huicholstudent]
    #28561447 - 11/29/23 10:00 PM (1 month, 27 days ago)

I put some spores to agar and PF+alder shaving cakes about a week ago. Most triched out, but one of the cakes is showing some weaker myc that I'm hopeful for. Excited to see this thread take off!


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OfflineLand TroutM
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Re: The Official Baeocystis Thread [Re: Fungus Gnat] * 4
    #28561493 - 11/29/23 10:33 PM (1 month, 27 days ago)

Quote:

Workman said:
Psilocybe baeocystis cultivated on sterile horse manure outdoors.  Spores of this species germinate and grow agonizingly slow on PDA and the colonization speed of manure is not much better.  But fruits form relatively quickly after planting outside.  Several experimental plots using woodchips (the expected substrate of choice) failed.  I can only assume that P. baeocystis is a secondary decomposer of wood chips and does poorly as the primary decomposer.





I'd like to thank mattso and Mjshroomer for providing the original spores from a specimen collected in Washington.

These fruits are from 2nd generation spores collected from a smaller fruiting last Fall.  Cultivation information on the Spore Works website will be updated to reflect the new results.

The skirtlike formation on the lower edge of the caps is not normally seen in P. baeocystis.  I also saw the same growth happen last year, but only in the first flush.  It may be the result of growing in such an overly rich substrate and normalizes after the excessive nutrients are used up.  It also may be strain specific.  I have another print to work with and see if there are any differences.

The Spore Works




I really take note that their efforts using a wood base sub failed.


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Invisiblethirdeyewild
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Re: The Official Baeocystis Thread [Re: Land Trout]
    #28561498 - 11/29/23 10:38 PM (1 month, 27 days ago)

That is interesting. My germinations on lme and sawdust are growing at a good speed. But in light of this I'll be sure to diversify my strategy.


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Invisiblekirkeng
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Re: The Official Baeocystis Thread [Re: thirdeyewild] * 1
    #28561537 - 11/29/23 11:37 PM (1 month, 27 days ago)

I got some rotted logs laying around, maybe scraping out the pulverized rotted wood could work? Or wood based compost? I’ll have to remember to collect some to try before things freeze over.


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Invisiblemind.at.large
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Re: The Official Baeocystis Thread [Re: thirdeyewild] * 1
    #28561538 - 11/29/23 11:38 PM (1 month, 27 days ago)

Ooh following along! I’m gonna have to bug one of y’all for some genetics soon!!


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Offlinehuicholstudent
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Re: The Official Baeocystis Thread [Re: mind.at.large]
    #28561548 - 11/30/23 12:02 AM (1 month, 27 days ago)

Thanks for bringing back these findings about P. Baeocystis being a secondary decomposer Land Trout. I seem to remember a couple other folks in the WL’s thread also sharing these observations. I feel like this is an important challenge to successfully getting an outdoor bed going. I can second that growth is extremely slow on agar. I’ve only made it to grain once and it stalled out and failed. I’m curious what others are thinking for an outdoor substrate if one gets viable grain spawn?


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Re: The Official Baeocystis Thread [Re: huicholstudent] * 5
    #28561558 - 11/30/23 12:15 AM (1 month, 27 days ago)



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OnlineSupaThaRipper
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Re: The Official Baeocystis Thread [Re: huicholstudent]
    #28561937 - 11/30/23 09:44 AM (1 month, 27 days ago)

@Nichrome
Messaged you and then seen this thread pop up on google
search. PERFECT

I hope Workman comments with some recent information. He seems to be the only one (that I could find) who’s had success with them.


Edited by SupaThaRipper (11/30/23 09:46 AM)


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OfflineWorkmanV
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Re: The Official Baeocystis Thread [Re: SupaThaRipper] * 7
    #28561957 - 11/30/23 09:59 AM (1 month, 27 days ago)

Good timing. I was just about to start some experiments.

I expect that the best tactic is to try as many strains/collections as possible to find one that is amenable to cultivation. Unfortunately, I just have one viable culture of unknown quality. If anyone can provide relatively fresh spores, even just a partial print, swab or gill fragment, I will put it in the rotation. The more samples, the higher chance of success. I am also looking locally, but it is a rare species I have only found once in the wild.

This effort will parallel with Psilocybe semilanceata tests, so any of those samples will also be accepted.

I'll document the effort and credit contributors (if any).

It is possible that Psilocybe venenata from Japan is actually P. baeocystis, so this thread might be of interest.

https://www.shroomery.org/forums/showflat.php/Number/4329423#4329423


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Edited by Workman (11/30/23 10:11 AM)


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OnlineSupaThaRipper
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Re: The Official Baeocystis Thread [Re: Workman]
    #28561965 - 11/30/23 10:06 AM (1 month, 27 days ago)

They’re in season now according to this

https://www.shroomery.org/12496/Psilocybe-baeocystis


And Alan has recently confirmed more findings on the east coast

https://www.shroomery.org/forums/showflat.php/Number/28255559

Good for someone in the area to go out and take a look


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Invisiblewavyedge

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Re: The Official Baeocystis Thread [Re: SupaThaRipper] * 2
    #28561999 - 11/30/23 10:53 AM (1 month, 27 days ago)

Aww shit! I'm in, for sure! :rockon:

This was my find in Oct, I took clones and spores that many of you now have:





Clones:




Temps around the start of fruiting:

(Scroll through Oct, pics are Oct 23)

https://www.timeanddate.com/weather/canada/burnaby/historic?month=9&year=2023



Edited by wavyedge (12/20/23 09:38 PM)


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Invisiblewavyedge

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Re: The Official Baeocystis Thread [Re: Workman]
    #28562006 - 11/30/23 11:00 AM (1 month, 27 days ago)

Quote:

Workman said:
Good timing. I was just about to start some experiments.

I expect that the best tactic is to try as many strains/collections as possible to find one that is amenable to cultivation. Unfortunately, I just have one viable culture of unknown quality. If anyone can provide relatively fresh spores, even just a partial print, swab or gill fragment, I will put it in the rotation. The more samples, the higher chance of success. I am also looking locally, but it is a rare species I have only found once in the wild.

This effort will parallel with Psilocybe semilanceata tests, so any of those samples will also be accepted.

I'll document the effort and credit contributors (if any).

It is possible that Psilocybe venenata from Japan is actually P. baeocystis, so this thread might be of interest.

https://www.shroomery.org/forums/showflat.php/Number/4329423#4329423






@workman hit me your addy I have a partial print still.


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OfflineNichrome
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Re: The Official Baeocystis Thread [Re: wavyedge] * 2
    #28562054 - 11/30/23 11:49 AM (1 month, 27 days ago)

Quote:

wavyedge said:
Aww shit! I'm in, for sure! :rockon:

This was my find in Oct, I took clones and spores that many of you now have:





Clones:



Temps around the start of fruiting:

(Scroll through Oct, pics are Oct 23)

https://www.timeanddate.com/weather/canada/burnaby/historic?month=9&year=2023






Mine is moving across MEA right now. Germinated in just a few short days. Thanks Wavy!

I have another LC that has sat on the shelf for the last 3 or 4 years with a very nice healthy white leather on top. I'll pull a little piece out and plate that up too. Those spores were collected in Bellingham, WA and the LC is an MS. The furthest I got with that culture was fully colonized grain. I wasn't able to get the grain to take to any kind of substrate though I didn't try a lot of things.

This is quickly getting more interesting.


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Better to be deprived of food for three days, than tea for one.


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Invisiblewavyedge

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Re: The Official Baeocystis Thread [Re: Nichrome] * 5
    #28562202 - 11/30/23 01:54 PM (1 month, 27 days ago)

I sat down and made some transfers from the clone plates today. I have been trying out these takeout dishes for agar plates that maybe have less waste plastic, and are definitely cheaper than the 100mm celltreat dishes I typically use:

https://www.amazon.ca/gp/aw/d/B07TK3NBLQ?ref=ppx_pt2_mob_b_prod_image



Thanks to @SupaThaRipper for that idea, seem to be working out real well, even better for experimenting with different substrates.

When I collected the specimens, I also collected about 1/2 L of the original sub, hoping to get it to grow at home. That turned into a gnat infested mess in the fridge, so I PC sterilized it in a ziplock round. I also made up ziplocks of hardwood sawdust and some pine shavings/bark.

I put some of those substrates into takeout plates and transferred from the "polar bear pubes" dish:





Edited by wavyedge (11/30/23 04:11 PM)


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OfflineLand TroutM
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Re: The Official Baeocystis Thread [Re: wavyedge] * 1
    #28562214 - 11/30/23 02:06 PM (1 month, 27 days ago)

That’s a pretty neat way of doing it, could probably vac seal them with a weight in the vac bag and drop that in a hot water bath for pasteurizing. I like the space you’re saving while doing several tests. That’s where I burn myself out, doing too many things over too much space and material.


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Invisiblewavyedge

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Re: The Official Baeocystis Thread [Re: Land Trout]
    #28562226 - 11/30/23 02:15 PM (1 month, 27 days ago)

One really nice part of those containers is they are sold as "leak proof" so I don't need to wrap the plate. That's what sold me on them.

Edit: sorry if I wasn't clear but these cups are disposable, polyethylene and come sterile in practice. I don't think they'd survive even pasteurization temps.

I sterilize the sub and used a sterile implement to move some into each cup. It does make me wonder if I could try to fruit directly from the cups...


Edited by wavyedge (11/30/23 03:34 PM)


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OfflineNichrome
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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28562566 - 11/30/23 05:56 PM (1 month, 27 days ago)

If somebody digs up and posts info on the phylogeny and any sequencing info, I'll tack it into the OP.


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Invisiblewavyedge

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Re: The Official Baeocystis Thread [Re: Nichrome] * 1
    #28562584 - 11/30/23 06:02 PM (1 month, 27 days ago)

Quote:

Nichrome said:
If somebody digs up and posts info on the phylogeny and any sequencing info, I'll tack it into the OP.




This feels like a relevant iNaturalist observation:

https://www.inaturalist.org/observations/5700142


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OfflineViridis420
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Re: The Official Baeocystis Thread [Re: wavyedge] * 4
    #28563986 - 12/01/23 04:15 PM (1 month, 26 days ago)

Sweet thread count me in!

Neosporen perhaps he'll chime in was successful growing baeos. Hit shaved maple tree wood with LC I believe.

I've been playing around with these of late using WBS for spawn. I had a couple stumps get ground down and removed. These shredded doug fir stumps mixed with Oregon ground clay soil is what I've been experimenting around with. I sterilized some of that and have a culture growing in 1/2 gallon sized jars. We'll see how that responds.

Also going to see about making planters of that mix with colonized spawn buried inside.

This particular baeos culture I'm playing with I got from a member out of Nova Scotia. Has these growing naturally in his yard lucky bastard around yard shrubs with wood chips spread about.

Here is a pix of how mine looks on WBS.


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Invisiblewavyedge

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Re: The Official Baeocystis Thread [Re: Viridis420]
    #28564002 - 12/01/23 04:31 PM (1 month, 26 days ago)

Sweet! Looks really good. How long did it take to colonize that wbs? Did you use lc?


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Invisibleelasticaltiger
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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28564015 - 12/01/23 04:40 PM (1 month, 26 days ago)

:threadmonitor:


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OfflineLand TroutM
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Re: The Official Baeocystis Thread [Re: Viridis420]
    #28564172 - 12/01/23 06:15 PM (1 month, 26 days ago)

Quote:

Viridis420 said:


Here is a pix of how mine looks on WBS.




That looks amazing compared to how mine looked.
I believe they were first described in Eugene Oregon.


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InvisibleHILLBILLY OUTLAWS
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Re: The Official Baeocystis Thread [Re: Nichrome]
    #28564477 - 12/01/23 09:24 PM (1 month, 25 days ago)

Good stuff. Can’t wait to see what everybody contributes to the thread. I’ll be lurking and watching!


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Re: The Official Baeocystis Thread [Re: HILLBILLY OUTLAW]
    #28564967 - 12/02/23 08:53 AM (1 month, 25 days ago)

:congrats:wow :popcorn:


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Invisiblethirdeyewild
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Re: The Official Baeocystis Thread [Re: Nichrome] * 3
    #28565157 - 12/02/23 11:42 AM (1 month, 25 days ago)

Broke open a punky Douglas fir and harvested it for substrate. Nice and clean, no dirt or needles. The big chunks crumble easily.

Those are large ziplocks


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Offlinehuicholstudent
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Re: The Official Baeocystis Thread [Re: thirdeyewild]
    #28565705 - 12/02/23 06:21 PM (1 month, 25 days ago)

Ok I just put some oldish spores to agar we’ll see what happens.

Thanks everyone for sharing their secondary decomposition substrate ideas. If my spores germinate I’ll probably try an old Fir stump as well since I have one in my yard.

For folks who have recently put spores to agar, how long is it taking to see growth on your plates?

Thanks everyone


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Re: The Official Baeocystis Thread [Re: huicholstudent]
    #28565719 - 12/02/23 06:27 PM (1 month, 25 days ago)

3 days. Mine are super fresh.


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Offlinehuicholstudent
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Re: The Official Baeocystis Thread [Re: thirdeyewild]
    #28565728 - 12/02/23 06:34 PM (1 month, 25 days ago)

Wow that’s quick! My spores are a few years old so I doubt they’ll germinate that rapidly. Looking forward to see how it goes once you inoculate the fir.


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Invisiblethirdeyewild
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Re: The Official Baeocystis Thread [Re: huicholstudent]
    #28565747 - 12/02/23 06:53 PM (1 month, 25 days ago)

Me to, if the mycelium likes it I'll go back to the tree, pulverize a lot of it and bury some spawn.


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Invisiblewavyedge

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Re: The Official Baeocystis Thread [Re: thirdeyewild] * 6
    #28566155 - 12/03/23 12:35 AM (1 month, 24 days ago)

Came back to life after the transfer, but will it leap?

Sawdust


Original substrate of find


Pine shavings and bark


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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28566174 - 12/03/23 01:47 AM (1 month, 24 days ago)

Nice I think sawdust looks good 👍 👌 :awedance::congrats:


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Invisiblealaskappalachian
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Re: The Official Baeocystis Thread [Re: rhizoRider] * 3
    #28574222 - 12/08/23 11:01 AM (1 month, 19 days ago)


Fun transfers to make this morning. These spores germed pretty fast for the temperature I'm keeping the room they're in.  Probably end up making Josex-poke LCs out of some T2s if these grow out clean enough. :skatebart:


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Invisiblewavyedge

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Re: The Official Baeocystis Thread [Re: alaskappalachian] * 2
    #28574247 - 12/08/23 11:15 AM (1 month, 19 days ago)

:thumbup: I think LC is the way to go with these, agar is agonizingly slow.


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Invisiblealaskappalachian
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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28574257 - 12/08/23 11:26 AM (1 month, 19 days ago)

That was my train of thought :noargument:  Thought maybe a handful of 250ml GWLCs >> small jars of compost/chips then to bulk pasteurized sawdust/chips/compost mix... Woodlovers always seem to uniformly cruise on unfiltered GWLC...


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Re: The Official Baeocystis Thread [Re: alaskappalachian] * 2
    #28574265 - 12/08/23 11:28 AM (1 month, 19 days ago)

Good to know, haven’t touched lc in a very long time but going to have to break my streak perhaps. Got germination on my plates a couple days ago!


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Re: The Official Baeocystis Thread [Re: kirkeng] * 3
    #28574486 - 12/08/23 02:24 PM (1 month, 19 days ago)

Pics hopefully tonight. Transferred pieces of "leather" from the top of an old ms lc (spores courtesy of NothingsChanged :hatsoff:) that's been sitting on the shelf for a couple years. The pieces are just starting to reach out into the agar. Also my Wavey MS is ready for T1's. Considering , as the growth looks clean, making an early lc from the germ plate.


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Re: The Official Baeocystis Thread [Re: wavyedge] * 4
    #28575989 - 12/09/23 03:23 PM (1 month, 18 days ago)

Quote:










After 10 days, these have barely progressed.



Whereas at least twice this much growth happened on dishes of low-nute grain water agar. They were taken at the same time.



I think it's time to start getting creative with substrates.


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Re: The Official Baeocystis Thread [Re: wavyedge] * 2
    #28576009 - 12/09/23 03:41 PM (1 month, 18 days ago)

I don't even know how I ended up here. :facepalm: I'm late. Too tired. It's cold. 
A little over an hour out from yelling at people on the drive home..
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Re: The Official Baeocystis Thread [Re: the_chosen_one] * 1
    #28576015 - 12/09/23 03:52 PM (1 month, 18 days ago)

Hey nice! A baeocystis thread :smile: Good one Nichrome!

No plans for them atm. However..think I might still have some spores from last year or the year before.


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Re: The Official Baeocystis Thread [Re: Tweeq] * 6
    #28576313 - 12/09/23 07:53 PM (1 month, 17 days ago)

So here's the old lc (note the dust layer :laugh2:) and the resulting transfers. These transfers are reaching out into the agar now and they look good.



Wavey's spore. Did 6 transfers from these as well as a no guts no glory germ plate lc (water and a little brf).


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Re: The Official Baeocystis Thread [Re: Nichrome] * 2
    #28581493 - 12/13/23 03:59 PM (1 month, 14 days ago)

Wavey's spores BRF LC is taking off pretty fast. By no means fast fast, but fast for the critter. Wavey's spore cultures are overall faster to move and less picky about nutrition than other baeos I've worked with.

Seems like the growth on BRF LC is more robust and faster compared to sugar based LC like LME or BRS. I whipped up some agar and added some BRF and dropped the sugar by 1% and added some extra brewers yeast to the mix and the myc seems to move faster with more robust growth on those plates too vs the 1.5% LPME Y agar I started them out on. I'll be continuing in that direction with the brf. I like what I'm seeing.


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Re: The Official Baeocystis Thread [Re: Nichrome] * 4
    #28583119 - 12/14/23 08:02 PM (1 month, 12 days ago)


That’s about how I remember it. Transfer from a plate that’s maybe two years old.


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Re: The Official Baeocystis Thread [Re: Land Trout] * 1
    #28586199 - 12/16/23 11:58 PM (1 month, 10 days ago)

How interesting... I just made up an ajar jar to 'noc with some beao spores I got from holo, then I saw this thread today... Turns out the print I have is of the slow weaker strain, so if anyone here's got a print (or partial) of wavy's strain I love to get some "under the scope". I've got an idea to try...


So what's the idea? Grass seed, the wonderful nutrient base who's good name has been unfairly besmirched for all times due to Violet/Anne's nonsense.

On the topic of natural habitat, while I've not hunted them myselves it seems that many of the photos of them show them growing in grassy areas. Y'all who have hunted them can maybe confirm or contradict this? If the general consensus seems to be that they are secondary decomposers, and Workman had success fruiting them on manure-based substrates, it seems to me that it may be either a grass/dung species that evolved to live off partially decomposed forest litter, or more likely a secondary forest litter species that learned to survive off grass-based nutrients due to deforestation (natural or anthropogenic).

It may just be me rambling because I made a big batch of dry-sift hash for the holidays, but these and ovoids seem to be species that are in the process of evolving due to catastrophic changes in climate. I'd blame Meltwater Pulse 1a and b for the evolutionary path of ovoids moving from forest litter to river debris, or another of the many great floods that have washed away great forests multiple times throughout geologic history. But while ovoids are common, beaos are more rare. To me this hints at them being younger in their evolutionary path, or at least slower because they are more secondary than primary.

Anyways, I guess what I'm trying to say is I'd like to try beao culture on grass seed soak water agar, and GS "grains" to test my hypothesis.
:lmafo:


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Re: The Official Baeocystis Thread [Re: ghiajake] * 5
    #28591420 - 12/20/23 01:38 PM (1 month, 7 days ago)

Well in my recent testing, rye grass seed soak agar performs slower for colonization than dilute grain soak agar.



On the left is grain (oats) cook water agar. Right is RGS cook water.

On my substrate tests, some baeo results: this species proves difficult when we ask it to jump from agar. These are all 8 days since transfer:

Straw pellets + coir



Starter mix:



Worm castings:



Other dishes are showing less or no growth.


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Re: The Official Baeocystis Thread [Re: wavyedge] * 2
    #28591433 - 12/20/23 01:50 PM (1 month, 7 days ago)

There's a few species on my "have to try in this lifetime" list. These are one of them. Fuck libs, fuck azurs, the Baeos are so rare and elusive that I just have to have this someday.

When I'm in a better position where I have some land I will reach out for spores but as of now there's no way I'd be able to make any meaningful contribution to this effort. Like maybe a guerrilla grow but like, who even does that.

This thread is so cool. Thank you to all who are working on this.


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Re: The Official Baeocystis Thread [Re: elasticaltiger]
    #28591442 - 12/20/23 01:57 PM (1 month, 7 days ago)

What is a guerilla grow? And please don’t tell me “indoors” lol because someone has to crack that case


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Re: The Official Baeocystis Thread [Re: SupaThaRipper]
    #28591448 - 12/20/23 02:03 PM (1 month, 7 days ago)

Guerilla grow is planting somewhere not on your property.


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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28591476 - 12/20/23 02:25 PM (1 month, 7 days ago)

Nope. Don’t like that!


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Re: The Official Baeocystis Thread [Re: SupaThaRipper]
    #28591487 - 12/20/23 02:34 PM (1 month, 7 days ago)

I've got several guerilla grows in the works. Part of diversifying my strategy.


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Re: The Official Baeocystis Thread [Re: thirdeyewild] * 2
    #28591502 - 12/20/23 02:46 PM (1 month, 7 days ago)

Fortunately my whole property is a guerilla grow. :lol: I can barely muster the motivation to pick Pelli's across the street right now.


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Re: The Official Baeocystis Thread [Re: the_chosen_one] * 4
    #28591549 - 12/20/23 03:31 PM (1 month, 7 days ago)

Is smelling like a gorilla while you do cult work the same thing?...  I'm not even guilty of that though.

T2s on my unfiltered brf poorly ground ugly agar twice as fast as on diluted oat soak broth.  Got 2 LCs going atm and like half a dozen T2s on various agar recipes.  Wish I had more small dishes to emulate wavyedge's strategy.  Gonna have to look around.  I'd ordered some of the cups supa started a thread about but they came crushed all to hell so have to get creative.


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Re: The Official Baeocystis Thread [Re: wavyedge] * 1
    #28591553 - 12/20/23 03:37 PM (1 month, 7 days ago)

Quote:

wavyedge said:
Guerilla grow is planting somewhere not on your property.




Not really. A guerilla grow is just a stealthy grow, whether it's on your property or not. All of my beds are "guerilla" because they are just mulched landscaped beds surrounding my house that would still be mulched if I wasn't growing fungi in them. Not one single person who didn't know they were spawned would suspect they are.


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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28591568 - 12/20/23 03:50 PM (1 month, 7 days ago)

Quote:

wavyedge said:
Well in my recent testing, rye grass seed soak agar performs slower for colonization than dilute grain soak agar.



On the left is grain (oats) cook water agar. Right is RGS cook water.






What was your Water:Soak Water ratio for each of those batches? The rye grass one seems quite darker than your oat grain one, implying that it is quite higher in nutrients. If you used the same ratio for both, then I'd have to say the grass seed leached out more nutrient during the soak than the oats did. Even if it's just that the grass seed contains more tannins than the oats, tannins are still a nutrient for basically all woodloving species. If it is a secondary decomposer it won't like the higher nutrient content.


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Re: The Official Baeocystis Thread [Re: ghiajake]
    #28591580 - 12/20/23 04:00 PM (1 month, 7 days ago)

Would be interesting to know the pH difference between your two agar types, if it was a tannin issue the rye seed one should have a lower pH than the oat one. Agar and grain pH is an issue that just doesn't get talked about enough when discussing cultures stalling out.


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Re: The Official Baeocystis Thread [Re: ghiajake]
    #28591585 - 12/20/23 04:12 PM (1 month, 7 days ago)

Has anyone in the group tried starving the culture? Since it apparently doesn't like high nutrients it would be nice to know the lower limit as well. From what I've seen so far all y'all's myc looks tomatose, finding the lower nutrient limit may cause the myc to flip rhizo.


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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28591593 - 12/20/23 04:20 PM (1 month, 7 days ago)

Quote:

wavyedge said:







This may just be a trick of lighting, but does the bottom right plate in your pick have radial leading edges from the two 'noc points where I put the red circles? I've seen that kind of "ghost edge" with Hericium sp. often.



Edited by ghiajake (12/20/23 04:32 PM)


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Re: The Official Baeocystis Thread [Re: ghiajake]
    #28591602 - 12/20/23 04:27 PM (1 month, 7 days ago)

Nope, myc extends just a tiny bit off the transfers.


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Re: The Official Baeocystis Thread [Re: ghiajake]
    #28591609 - 12/20/23 04:31 PM (1 month, 7 days ago)

Quote:

ghiajake said:
Quote:

wavyedge said:
Guerilla grow is planting somewhere not on your property.




Not really. A guerilla grow is just a stealthy grow, whether it's on your property or not. All of my beds are "guerilla" because they are just mulched landscaped beds surrounding my house that would still be mulched if I wasn't growing fungi in them. Not one single person who didn't know they were spawned would suspect they are.





https://en.m.wikipedia.org/wiki/Guerrilla_gardening


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Re: The Official Baeocystis Thread [Re: wavyedge] * 2
    #28591711 - 12/20/23 05:27 PM (1 month, 7 days ago)

Quote:

wavyedge said:
Quote:

ghiajake said:
Quote:

wavyedge said:
Guerilla grow is planting somewhere not on your property.




Not really. A guerilla grow is just a stealthy grow, whether it's on your property or not. All of my beds are "guerilla" because they are just mulched landscaped beds surrounding my house that would still be mulched if I wasn't growing fungi in them. Not one single person who didn't know they were spawned would suspect they are.





https://en.m.wikipedia.org/wiki/Guerrilla_gardening





I guess that depends on whether you consider growing psilocybe mushrooms to be "gardening", which I do not. They are not "food, plants, or flowers" to use your link's definition, and since fungi are more related to us than plants you can't appropriately use plant terms at all for cultivating them.

I get that that is more my own Apsie issue than other people care to get into, so let's just look at the relative definition of the word "guerilla" when used as an adjective. Copied from Cambridge's website:

guerrilla
adjective [ before noun ]
UK  /ɡəˈrɪl.ə/ US  /ɡəˈrɪl.ə/

using unusual methods to create or get attention for your ideas, art, products, etc., usually ones that cost little money and involve using public spaces:



Yes, it does mention public spaces. But it does not mention using other people's private property like your wiki definition does. That's just illegal trespassing. Notice it says "usually" (not "always"), but begins by saying "using unusual methods". That's a bit of an oxymoron since if people "usually" use public spaces, then the "unusual method" would be to use your own spaces. :wink: Besides, building a mushroom bed that mimics a natural bed somewhere that's not on you property is not "guerilla" because it is the usual method they grow anyways, not the "unusual method".

Technically "guerilla gardening" uses "guerilla" as an adverb since "gardening" is a verb, which it does not have a definition for and is not a correct way to use the adjective. Sure, "guerilla garden", "guerilla grow", and "guerilla warfare" are correct usages since they precede nouns, but it defaults back to the standard definition I listed above when used as an adjective. 

Anyways, I'm sure nobody cares about about the correct usage and definitions of terms like I do. The point is that "guerilla" boils down to "unusual methods" no matter how it's used, and I argue that mimicking natural processes in areas that they would normally grow anyways if they were local to your region does not fall under the definitions of "guerilla" or "gardening". It's simply just "wild propagation", technically no different than if an animal (or human) ate a fruit and puked/shat it out in a new area for the spores to germinate and colonize.


Edited by ghiajake (12/20/23 05:41 PM)


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Re: The Official Baeocystis Thread [Re: ghiajake] * 1
    #28592144 - 12/21/23 12:24 AM (1 month, 6 days ago)

Thank you for the etymology lesson on those terms. I learned some stuff and broadened my understanding of my born language and words I use regularly. I love both the origins of elements of language as well as their evolution through time and into the future. Through our interactions we create an evolving vernacular.

If the term guerrilla grow is used in context to a certain vernacular, and is used properly pertaining to that vernacular, then the usage is valid. It would fly in a modern college paper. So would the term fly if contextualized in the same vernacular. Guerrilla growing is well defined and well used in the modern American counterculture vernacular.

In that sense I have done a lot of guerrilla grows and enjoy it very much. My mulch, public places, natural spaces, etc. I was spreading cyanescens around Olympia Wa parks and trails as far back as 01. I try to bring spawn to the huge city mulch piles every year still. That mulch is eventually sold by the city to bag mulch companies that show up with dozens of semi trucks. Then it gets sold worldwide. Doing my part.

So back to the topic at hand, my brf LC is doing splendidly. It will be ready to fill bank vials and play with grains and substrates soon enough. This Wavy spore culture is the fastest baeo I have seen yet.

In the 20's American jazz vernacular, I'm a viper and I'm going to get busy smokin' tea. :hatsoff:


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Re: The Official Baeocystis Thread [Re: Nichrome] * 5
    #28592696 - 12/21/23 12:31 PM (1 month, 6 days ago)

T1 s are taking well it seems, bottom right is a tad funky comparatively. Agar mix is MYA at like 1.4%, I’m not noticing a major drop off in speed. Definitely slower than say cyans but is growing at about the speed of my caerulipes or papuana.



Been gathering some rotted leaves and wood around the yard to use down the line for tests.


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Re: The Official Baeocystis Thread [Re: Nichrome]
    #28592704 - 12/21/23 12:41 PM (1 month, 6 days ago)

Quote:

Nichrome said:
Thank you for the etymology lesson on those terms. I learned some stuff and broadened my understanding of my born language and words I use regularly. I love both the origins of elements of language as well as their evolution through time and into the future. Through our interactions we create an evolving vernacular.

If the term guerrilla grow is used in context to a certain vernacular, and is used properly pertaining to that vernacular, then the usage is valid. It would fly in a modern college paper. So would the term fly if contextualized in the same vernacular. Guerrilla growing is well defined and well used in the modern American counterculture vernacular.

In that sense I have done a lot of guerrilla grows and enjoy it very much. My mulch, public places, natural spaces, etc. I was spreading cyanescens around Olympia Wa parks and trails as far back as 01. I try to bring spawn to the huge city mulch piles every year still. That mulch is eventually sold by the city to bag mulch companies that show up with dozens of semi trucks. Then it gets sold worldwide. Doing my part.

So back to the topic at hand, my brf LC is doing splendidly. It will be ready to fill bank vials and play with grains and substrates soon enough. This Wavy spore culture is the fastest baeo I have seen yet.

In the 20's American jazz vernacular, I'm a viper and I'm going to get busy smokin' tea. :hatsoff:




I'd argue that "American counterculture vernacular" is just a fancier PC term for the word "slang", but like I said in my reply I totally understand that's more of me being an Apsie than me expecting others to agree with me.

But as you said, back to the main topic... I mentioned in one of my replies to wavy that it would be interesting to know the pH differences between his two types of agar, since that's a topic not usually discussed when talking about cultures stalling. Since you're having good results with BRF LC, have you thought to check your LC's pH?


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Re: The Official Baeocystis Thread [Re: ghiajake] * 1
    #28594624 - 12/22/23 10:57 PM (1 month, 4 days ago)

Nice thread, very cool species!


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Re: The Official Baeocystis Thread [Re: YoshiTrainer] * 2
    #28597942 - 12/25/23 08:15 PM (1 month, 1 day ago)

Having some better progress on rye, this time from LC.

Better colonization of the rye grain than I saw with oats. 7 days growth, there's more than shows in the picture, and I could do much better with LC (I'm a noob in that dept.)



Edited by wavyedge (12/25/23 08:16 PM)


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Re: The Official Baeocystis Thread [Re: wavyedge] * 1
    #28599728 - 12/27/23 11:49 AM (1 month, 7 hours ago)

Has anyone experimented with different colonization temps? All of my attempts have been growing super slow, but I nocc'd another plate on 12/10, didn't see any growth on 12/21, then left for the holidays and had my house cooler at 58F. Came back and the plate has way more growth than any of my previous agar runs. It seems a little unlikely, but maybe it does a bit better in cooler weather.


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Re: The Official Baeocystis Thread [Re: Fungus Gnat] * 4
    #28602748 - 12/29/23 09:30 PM (28 days, 21 hours ago)

I’ll try that. I put a makarorae jar in the garage a couple days ago to see if it would make a difference, I’ll put one of my baeo jars out there too.

This one was knocked up on he 2nd, it was in the fridge for about a week.


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Re: The Official Baeocystis Thread [Re: ghiajake] * 2
    #28602758 - 12/29/23 09:48 PM (28 days, 21 hours ago)



Looks like my BRF and well water LC brine is right about neutral out of the pc and cooled to room temperature.

The Baeo LC is now mature and grew about as fast as a cube LC in the same recipe made the same day. Lightning speed.

It will go to rye very soon.


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Re: The Official Baeocystis Thread [Re: Nichrome] * 1
    #28603005 - 12/30/23 06:52 AM (28 days, 12 hours ago)

Quote:

Nichrome said:


Looks like my BRF and well water LC brine is right about neutral out of the pc and cooled to room temperature.

The Baeo LC is now mature and grew about as fast as a cube LC in the same recipe made the same day. Lightning speed.

It will go to rye very soon.




Right on, thanks for checking.


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Invisiblealaskappalachian
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Re: The Official Baeocystis Thread [Re: ghiajake] * 4
    #28615468 - 01/09/24 04:32 PM (18 days, 2 hours ago)

LC #1 kicking off quicker than I thought. (As fast as my melmac and yeti from the same session 3 days ago-ish).  Shitty broth too (only had brown sugar (-w- inverse sugar)) and a pinch of yeast, so I was glad to see it do something.  Making some brf lcs tonight for more baeo xfers, and I suspect they'll move almost twice as fast on it and colonize way thicker in the end.  Made new agar with 50:50 WBR boil broth:H20 to thicken up these cultures too


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OfflineNichrome
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Re: The Official Baeocystis Thread [Re: alaskappalachian] * 3
    #28635075 - 01/25/24 10:27 PM (1 day, 20 hours ago)

I put some on grain and it is moving insanely slow and thin, almost invisible but it is moving.

I have 4 jars I noc'd with wild caught axenic Pseudomonas fluorescns bacteria lc. I snatched the pseudomonas off a Cordyceps militaris fruit I found in July in NH last year. I'll let those go a while then add the Baeo lc. Also going to noc another 4 with microscopic slime mold I pulled off my kiwi vines. I equipped the culture in it's amoeba stage with the same P fluorescens culture and now several generations in when the spores from the slime germinate and are in their amoeba form, the same Pseudomonas is still also present. If you magnify the slime spores enough you can see the little bacteria packets. P fluorescens unlocks iron and does all sorts of other wonderful things. Well see how the baeos like grain with pseudomonas, and grain with pseudomonas equipped slime molds.

This type of slime mold is invisible to the naked eye but is common in decaying plant material such as wood mulch. I often see slime molds in cohabitation with wild psilocybe patches. I've seen them eat psilo patches too like conquering invaders, but typically the big puke looking ones not the little tiny ones. The micro ones seem to get along hence my inspiration for the fucking around.


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Better to be deprived of food for three days, than tea for one.


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Invisiblekirkeng
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Re: The Official Baeocystis Thread [Re: Nichrome]
    #28635085 - 01/25/24 10:58 PM (1 day, 20 hours ago)

I’m looking forward to you finding out! Very cool idea man.


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Offlineendemic_entheogens
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Re: The Official Baeocystis Thread [Re: Nichrome]
    #28635094 - 01/25/24 11:07 PM (1 day, 20 hours ago)

Have you seen any other cultures shown improvement from having another organism thrown in with the grain spawn?
Curious because I feel that is why contaminated spawn does better outdoors and why pasteurization is better than sterilization in some cases. Seems like a mountain of a project to actually find what organisms help tho.


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OfflineNichrome
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Re: The Official Baeocystis Thread [Re: endemic_entheogens] * 1
    #28635099 - 01/25/24 11:18 PM (1 day, 19 hours ago)

Happens all the time. Lot's of things do better with each other.

For example the fungi that leaf cutter ants lost it's ability to make new spores millions of years ago. The fungus has been handed from ant to ant for close to 50 million years now by modern reckoning. Gongylidia...


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Better to be deprived of food for three days, than tea for one.


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Offlineendemic_entheogens
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Re: The Official Baeocystis Thread [Re: Nichrome] * 1
    #28635106 - 01/25/24 11:30 PM (1 day, 19 hours ago)

Oh absolutely, I was more curious in a modern mush cult sense if anyone has identified or been working with microorganisms that aid in psilocybe growth or immune function.


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Re: The Official Baeocystis Thread [Re: endemic_entheogens] * 3
    #28635119 - 01/26/24 12:06 AM (1 day, 19 hours ago)

There is very little legal research happening with psilocybes in our modern world.

You can find plenty of info on trichoderma preventing the growth of claviceps on cereal grains via volatile off gassing amines, but there isn't a lot to go on with psilocybes.

Very few of the people working with psilocybes in the real world, like the fine folks here for example, are working with bacteria or mold of any sort. I very rarely see anybody here intentionally growing bacteria, amoebas, mites, mold, nematodes, or any other microorganisms even though many people here posses the skill to do so. Understandably because many bacteria and molds and microorganisms are deathly scary, potentially pathogenic, and very hard to identify without expensive lab gear such as nanopore sequencers, liquid and gas chromatography, and extensive taxonomic knowledge or at least access there to. This place is likely the leading observational hub for modern psilocybe research too. It may get there eventually with the whole symbiosis thing. There is no rush in knowing everything there is to know. I enjoy the ride.

This all seems to be modernized but what we are all doing is still in it's infancy.


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Re: The Official Baeocystis Thread [Re: Nichrome] * 4
    #28635128 - 01/26/24 12:20 AM (1 day, 18 hours ago)



--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineLand TroutM
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Re: The Official Baeocystis Thread [Re: Nichrome] * 4
    #28635703 - 01/26/24 02:49 PM (1 day, 4 hours ago)

hope to send these to substrate Sunday, or tomorrow night. Inoculated on 12/2 with wedges from a very old plate. I just Bagged up  a blend of different organic material I had around. Coir, worm castings, aged manure, decomposed fir bark, spend oyster blocks, grass clippings and such. The grain has been ready for awhile, I just haven’t had the time to get the substrates ready, but I feel pretty good about it.


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Re: The Official Baeocystis Thread [Re: Land Trout]
    #28635710 - 01/26/24 02:55 PM (1 day, 4 hours ago)

That looks nice bro:popcorn:


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Re: The Official Baeocystis Thread [Re: rhizoRider]
    #28635750 - 01/26/24 03:35 PM (1 day, 3 hours ago)

:whathesaid:


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Re: The Official Baeocystis Thread [Re: Nichrome] * 4
    #28636343 - 01/26/24 11:53 PM (19 hours, 19 minutes ago)

Put this round to my grain mix from agar on the 15th. Much of the grain shows growth.



Also spawned into straw pellets, hwfp, cow manure coir mix.

Growth is visible after 2 days.



Will it stall or will it colonize fully, that's the question.


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Re: The Official Baeocystis Thread [Re: wavyedge]
    #28636913 - 01/27/24 12:55 PM (6 hours, 17 minutes ago)

That looks robust.


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Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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Re: The Official Baeocystis Thread [Re: Nichrome]
    #28637037 - 01/27/24 03:00 PM (4 hours, 13 minutes ago)


Should I take worms out of leave them in before I sterilize.


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OnlineSupaThaRipper
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Re: The Official Baeocystis Thread [Re: Land Trout]
    #28637046 - 01/27/24 03:07 PM (4 hours, 5 minutes ago)

🤣 poor guy


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