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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Aww shit! I'm in, for sure! 
This was my find in Oct, I took clones and spores that many of you now have:


Clones:

Temps around the start of fruiting:
(Scroll through Oct, pics are Oct 23)
https://www.timeanddate.com/weather/canada/burnaby/historic?month=9&year=2023
Edited by wavyedge (12/20/23 09:38 PM)
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: Workman]
#28562006 - 11/30/23 11:00 AM (1 month, 27 days ago) |
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Quote:
Workman said: Good timing. I was just about to start some experiments.
I expect that the best tactic is to try as many strains/collections as possible to find one that is amenable to cultivation. Unfortunately, I just have one viable culture of unknown quality. If anyone can provide relatively fresh spores, even just a partial print, swab or gill fragment, I will put it in the rotation. The more samples, the higher chance of success. I am also looking locally, but it is a rare species I have only found once in the wild.
This effort will parallel with Psilocybe semilanceata tests, so any of those samples will also be accepted.
I'll document the effort and credit contributors (if any).
It is possible that Psilocybe venenata from Japan is actually P. baeocystis, so this thread might be of interest.
https://www.shroomery.org/forums/showflat.php/Number/4329423#4329423
@workman hit me your addy I have a partial print still.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: Nichrome] 5
#28562202 - 11/30/23 01:54 PM (1 month, 27 days ago) |
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I sat down and made some transfers from the clone plates today. I have been trying out these takeout dishes for agar plates that maybe have less waste plastic, and are definitely cheaper than the 100mm celltreat dishes I typically use:
https://www.amazon.ca/gp/aw/d/B07TK3NBLQ?ref=ppx_pt2_mob_b_prod_image

Thanks to @SupaThaRipper for that idea, seem to be working out real well, even better for experimenting with different substrates.
When I collected the specimens, I also collected about 1/2 L of the original sub, hoping to get it to grow at home. That turned into a gnat infested mess in the fridge, so I PC sterilized it in a ziplock round. I also made up ziplocks of hardwood sawdust and some pine shavings/bark.
I put some of those substrates into takeout plates and transferred from the "polar bear pubes" dish:

Edited by wavyedge (11/30/23 04:11 PM)
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: Land Trout]
#28562226 - 11/30/23 02:15 PM (1 month, 27 days ago) |
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One really nice part of those containers is they are sold as "leak proof" so I don't need to wrap the plate. That's what sold me on them.
Edit: sorry if I wasn't clear but these cups are disposable, polyethylene and come sterile in practice. I don't think they'd survive even pasteurization temps.
I sterilize the sub and used a sterile implement to move some into each cup. It does make me wonder if I could try to fruit directly from the cups...
Edited by wavyedge (11/30/23 03:34 PM)
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: Nichrome] 1
#28562584 - 11/30/23 06:02 PM (1 month, 27 days ago) |
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Quote:
Nichrome said: If somebody digs up and posts info on the phylogeny and any sequencing info, I'll tack it into the OP.
This feels like a relevant iNaturalist observation:
https://www.inaturalist.org/observations/5700142
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: Viridis420]
#28564002 - 12/01/23 04:31 PM (1 month, 26 days ago) |
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Sweet! Looks really good. How long did it take to colonize that wbs? Did you use lc?
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: thirdeyewild] 6
#28566155 - 12/03/23 12:35 AM (1 month, 25 days ago) |
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Came back to life after the transfer, but will it leap?
Sawdust

Original substrate of find

Pine shavings and bark
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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I think LC is the way to go with these, agar is agonizingly slow.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: wavyedge] 4
#28575989 - 12/09/23 03:23 PM (1 month, 18 days ago) |
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Quote:


After 10 days, these have barely progressed.

Whereas at least twice this much growth happened on dishes of low-nute grain water agar. They were taken at the same time.

I think it's time to start getting creative with substrates.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: ghiajake] 5
#28591420 - 12/20/23 01:38 PM (1 month, 7 days ago) |
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Well in my recent testing, rye grass seed soak agar performs slower for colonization than dilute grain soak agar.

On the left is grain (oats) cook water agar. Right is RGS cook water.
On my substrate tests, some baeo results: this species proves difficult when we ask it to jump from agar. These are all 8 days since transfer:
Straw pellets + coir

Starter mix:

Worm castings:

Other dishes are showing less or no growth.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Guerilla grow is planting somewhere not on your property.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: ghiajake]
#28591602 - 12/20/23 04:27 PM (1 month, 7 days ago) |
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Nope, myc extends just a tiny bit off the transfers.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: ghiajake]
#28591609 - 12/20/23 04:31 PM (1 month, 7 days ago) |
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Quote:
ghiajake said:
Quote:
wavyedge said: Guerilla grow is planting somewhere not on your property.
Not really. A guerilla grow is just a stealthy grow, whether it's on your property or not. All of my beds are "guerilla" because they are just mulched landscaped beds surrounding my house that would still be mulched if I wasn't growing fungi in them. Not one single person who didn't know they were spawned would suspect they are.
https://en.m.wikipedia.org/wiki/Guerrilla_gardening
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: YoshiTrainer] 2
#28597942 - 12/25/23 08:15 PM (1 month, 2 days ago) |
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Having some better progress on rye, this time from LC.
Better colonization of the rye grain than I saw with oats. 7 days growth, there's more than shows in the picture, and I could do much better with LC (I'm a noob in that dept.)
Edited by wavyedge (12/25/23 08:16 PM)
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: Nichrome] 4
#28636343 - 01/26/24 11:53 PM (1 day, 8 hours ago) |
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Put this round to my grain mix from agar on the 15th. Much of the grain shows growth.

Also spawned into straw pellets, hwfp, cow manure coir mix.
Growth is visible after 2 days.

Will it stall or will it colonize fully, that's the question.
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