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Nichrome
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The Official Baeocystis Thread 13
#28561041 - 11/29/23 04:57 PM (1 month, 28 days ago) |
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P. baeocystis is definitely one of the more fascinating species of active mushrooms. I fell in love with them decades ago when I would find them amongst other woodlovers in the fall. They were always so mysterious in their nature and the way they conduct themselves. Upon eating them for the first time I realized just how special they really are.
This species has largely escaped the modern mushroom boom. Something in their elusive nature that carries through I guess. Not only do they evade our attention but those of us who have tried working with them have found it increasingly more mysterious and downright difficult. You can't spell difficult without cult.
This is a thread dedicated to the lovely Psilocybe Baeocystis in all it's glory. Let's work together to open the box on this species and let it's secrets out. It is a strong one, and I think right now there is enough community gusto to crack the codes and let loose on the world what this beautiful species has to teach us.
(I do plan to edit the op with more info and links and such)
-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Workman
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Good timing. I was just about to start some experiments.
I expect that the best tactic is to try as many strains/collections as possible to find one that is amenable to cultivation. Unfortunately, I just have one viable culture of unknown quality. If anyone can provide relatively fresh spores, even just a partial print, swab or gill fragment, I will put it in the rotation. The more samples, the higher chance of success. I am also looking locally, but it is a rare species I have only found once in the wild.
This effort will parallel with Psilocybe semilanceata tests, so any of those samples will also be accepted.
I'll document the effort and credit contributors (if any).
It is possible that Psilocybe venenata from Japan is actually P. baeocystis, so this thread might be of interest.
https://www.shroomery.org/forums/showflat.php/Number/4329423#4329423
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
Edited by Workman (11/30/23 10:11 AM)
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wavyedge

Registered: 09/24/11
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Loc: Canada
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Re: The Official Baeocystis Thread [Re: thirdeyewild] 6
#28566155 - 12/03/23 12:35 AM (1 month, 25 days ago) |
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Came back to life after the transfer, but will it leap?
Sawdust

Original substrate of find

Pine shavings and bark
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Nichrome
I'm a torso!


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Re: The Official Baeocystis Thread [Re: Tweeq] 6
#28576313 - 12/09/23 07:53 PM (1 month, 18 days ago) |
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So here's the old lc (note the dust layer ) and the resulting transfers. These transfers are reaching out into the agar now and they look good.

Wavey's spore. Did 6 transfers from these as well as a no guts no glory germ plate lc (water and a little brf).

-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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huicholstudent
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wavyedge

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Re: The Official Baeocystis Thread [Re: Nichrome] 5
#28562202 - 11/30/23 01:54 PM (1 month, 27 days ago) |
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I sat down and made some transfers from the clone plates today. I have been trying out these takeout dishes for agar plates that maybe have less waste plastic, and are definitely cheaper than the 100mm celltreat dishes I typically use:
https://www.amazon.ca/gp/aw/d/B07TK3NBLQ?ref=ppx_pt2_mob_b_prod_image

Thanks to @SupaThaRipper for that idea, seem to be working out real well, even better for experimenting with different substrates.
When I collected the specimens, I also collected about 1/2 L of the original sub, hoping to get it to grow at home. That turned into a gnat infested mess in the fridge, so I PC sterilized it in a ziplock round. I also made up ziplocks of hardwood sawdust and some pine shavings/bark.
I put some of those substrates into takeout plates and transferred from the "polar bear pubes" dish:

Edited by wavyedge (11/30/23 04:11 PM)
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wavyedge

Registered: 09/24/11
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Loc: Canada
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Re: The Official Baeocystis Thread [Re: ghiajake] 5
#28591420 - 12/20/23 01:38 PM (1 month, 7 days ago) |
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Well in my recent testing, rye grass seed soak agar performs slower for colonization than dilute grain soak agar.

On the left is grain (oats) cook water agar. Right is RGS cook water.
On my substrate tests, some baeo results: this species proves difficult when we ask it to jump from agar. These are all 8 days since transfer:
Straw pellets + coir

Starter mix:

Worm castings:

Other dishes are showing less or no growth.
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kirkeng
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Re: The Official Baeocystis Thread [Re: Nichrome] 5
#28592696 - 12/21/23 12:31 PM (1 month, 6 days ago) |
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T1 s are taking well it seems, bottom right is a tad funky comparatively. Agar mix is MYA at like 1.4%, I’m not noticing a major drop off in speed. Definitely slower than say cyans but is growing at about the speed of my caerulipes or papuana.

Been gathering some rotted leaves and wood around the yard to use down the line for tests.
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Land Trout
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Re: The Official Baeocystis Thread [Re: Fungus Gnat] 4
#28561493 - 11/29/23 10:33 PM (1 month, 28 days ago) |
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Quote:
Workman said: Psilocybe baeocystis cultivated on sterile horse manure outdoors. Spores of this species germinate and grow agonizingly slow on PDA and the colonization speed of manure is not much better. But fruits form relatively quickly after planting outside. Several experimental plots using woodchips (the expected substrate of choice) failed. I can only assume that P. baeocystis is a secondary decomposer of wood chips and does poorly as the primary decomposer.


I'd like to thank mattso and Mjshroomer for providing the original spores from a specimen collected in Washington.
These fruits are from 2nd generation spores collected from a smaller fruiting last Fall. Cultivation information on the Spore Works website will be updated to reflect the new results.
The skirtlike formation on the lower edge of the caps is not normally seen in P. baeocystis. I also saw the same growth happen last year, but only in the first flush. It may be the result of growing in such an overly rich substrate and normalizes after the excessive nutrients are used up. It also may be strain specific. I have another print to work with and see if there are any differences.
The Spore Works
I really take note that their efforts using a wood base sub failed.
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Viridis420

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Re: The Official Baeocystis Thread [Re: wavyedge] 4
#28563986 - 12/01/23 04:15 PM (1 month, 26 days ago) |
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Sweet thread count me in!
Neosporen perhaps he'll chime in was successful growing baeos. Hit shaved maple tree wood with LC I believe.
I've been playing around with these of late using WBS for spawn. I had a couple stumps get ground down and removed. These shredded doug fir stumps mixed with Oregon ground clay soil is what I've been experimenting around with. I sterilized some of that and have a culture growing in 1/2 gallon sized jars. We'll see how that responds.
Also going to see about making planters of that mix with colonized spawn buried inside.
This particular baeos culture I'm playing with I got from a member out of Nova Scotia. Has these growing naturally in his yard lucky bastard around yard shrubs with wood chips spread about.
Here is a pix of how mine looks on WBS.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: wavyedge] 4
#28575989 - 12/09/23 03:23 PM (1 month, 18 days ago) |
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Quote:


After 10 days, these have barely progressed.

Whereas at least twice this much growth happened on dishes of low-nute grain water agar. They were taken at the same time.

I think it's time to start getting creative with substrates.
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Land Trout
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Re: The Official Baeocystis Thread [Re: Nichrome] 4
#28583119 - 12/14/23 08:02 PM (1 month, 13 days ago) |
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 That’s about how I remember it. Transfer from a plate that’s maybe two years old.
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alaskappalachian
Entitiologist

Registered: 10/22/19
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Loc: The 49th Dimension
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Is smelling like a gorilla while you do cult work the same thing?... I'm not even guilty of that though.
 T2s on my unfiltered brf poorly ground ugly agar twice as fast as on diluted oat soak broth. Got 2 LCs going atm and like half a dozen T2s on various agar recipes. Wish I had more small dishes to emulate wavyedge's strategy. Gonna have to look around. I'd ordered some of the cups supa started a thread about but they came crushed all to hell so have to get creative.
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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Land Trout
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Re: The Official Baeocystis Thread [Re: Fungus Gnat] 4
#28602748 - 12/29/23 09:30 PM (29 days, 9 hours ago) |
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I’ll try that. I put a makarorae jar in the garage a couple days ago to see if it would make a difference, I’ll put one of my baeo jars out there too.
 This one was knocked up on he 2nd, it was in the fridge for about a week.
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alaskappalachian
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Re: The Official Baeocystis Thread [Re: ghiajake] 4
#28615468 - 01/09/24 04:32 PM (18 days, 14 hours ago) |
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LC #1 kicking off quicker than I thought. (As fast as my melmac and yeti from the same session 3 days ago-ish). Shitty broth too (only had brown sugar (-w- inverse sugar)) and a pinch of yeast, so I was glad to see it do something. Making some brf lcs tonight for more baeo xfers, and I suspect they'll move almost twice as fast on it and colonize way thicker in the end. Made new agar with 50:50 WBR boil broth:H20 to thicken up these cultures too
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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Nichrome
I'm a torso!


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Re: The Official Baeocystis Thread [Re: Nichrome] 4
#28635128 - 01/26/24 12:20 AM (2 days, 7 hours ago) |
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-------------------- “Better to be deprived of food for three days, than tea for one.”
Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson
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Land Trout
Stranger



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Re: The Official Baeocystis Thread [Re: Nichrome] 4
#28635703 - 01/26/24 02:49 PM (1 day, 16 hours ago) |
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hope to send these to substrate Sunday, or tomorrow night. Inoculated on 12/2 with wedges from a very old plate. I just Bagged up a blend of different organic material I had around. Coir, worm castings, aged manure, decomposed fir bark, spend oyster blocks, grass clippings and such. The grain has been ready for awhile, I just haven’t had the time to get the substrates ready, but I feel pretty good about it.
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wavyedge

Registered: 09/24/11
Posts: 362
Loc: Canada
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Re: The Official Baeocystis Thread [Re: Nichrome] 4
#28636343 - 01/26/24 11:53 PM (1 day, 7 hours ago) |
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Put this round to my grain mix from agar on the 15th. Much of the grain shows growth.

Also spawned into straw pellets, hwfp, cow manure coir mix.
Growth is visible after 2 days.

Will it stall or will it colonize fully, that's the question.
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thirdeyewild



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Re: The Official Baeocystis Thread [Re: Nichrome] 3
#28561056 - 11/29/23 05:08 PM (1 month, 28 days ago) |
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Good call! I'm in. About a week in from germinating my first baeo spores and I'm thrilled to be able to work with these. Never tried them but spent many fruitless hours looking for them. Hope to be able to pass some spores along one day.
Let's hope we can crack these like the community has so many other exotics.
Edited by thirdeyewild (11/29/23 05:11 PM)
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alaskappalachian
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Re: The Official Baeocystis Thread [Re: thirdeyewild] 3
#28561064 - 11/29/23 05:12 PM (1 month, 28 days ago) |
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Wonderful idea. Streaked my plates this morning. It was the photo of these in the Audubon Field Guide I got when I was 12 that inspired my interest in psilocybe species, and being able to attempt cultivating these is a dream come true. Can't wait to see what is contributed here...
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
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