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Offlinecatalog99
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Chestnut Mushroom (pholiota adiposa) metabolites
    #28552648 - 11/22/23 10:21 AM (2 months, 5 days ago)

I bought some chestnut mushrooms from a local vendor.  I cloned the chestnut into LC.  I grew the LC out on agar and it looked clean.  So I inoculated some rye grain jars.  Two of the jars came out with some metabolites in them. 

See picture.


I wonder whether the LC is contaminated or, for some reason, has a lot of metabolites.  When I inoculated rye jars with G2G inoculation, they look perfectly white (no metabolites). 

Also, I did a shake on jars of rye that were inoculated with the same chestnut LC, see the right most jar in pict above.  Two of the jars did not recover after the shake.

So I wonder why LC is not working as well as G2G inoculation for chestnut?
Any ideas?


Edited by catalog99 (11/22/23 10:24 AM)


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OfflineStromriderM
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Re: Chestnut Mushroom (pholiota adiposa) metabolites [Re: catalog99]
    #28552888 - 11/22/23 02:04 PM (2 months, 5 days ago)

Why add the extra step? Just drop an agar wedge into the grain and you won't have to worry about silly stuff

That spawn doesn't look great. Chestnuts certainly like to make metabolites but that doesn't look right to me


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Offlinecatalog99
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Re: Chestnut Mushroom (pholiota adiposa) metabolites [Re: Stromrider]
    #28553653 - 11/23/23 08:00 AM (2 months, 4 days ago)

I used the Chestnut LC to expand the mycelium from a clone in a Petri dish.  I suspect that LC does not work well with Chestnuts.  Any comments on that?


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Re: Chestnut Mushroom (pholiota adiposa) metabolites [Re: catalog99]
    #28554249 - 11/23/23 05:42 PM (2 months, 3 days ago)

My recommendation is skip the lc. Inoculate your grains with an agar wedge and expand using grain to grain transfer


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