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OfflineJust_a_Shadow
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Autoclavable bag
    #2852455 - 07/02/04 09:32 PM (17 years, 4 months ago)

I just got this autoclaveable bag from the net, does anyone know any teks I could use with it?


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OfflineSev
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Re: Autoclavable bag [Re: Just_a_Shadow]
    #2852546 - 07/02/04 10:29 PM (17 years, 4 months ago)

:thumbdown: :thumbdown: :thumbdown: :shake:


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"Do we want the stars? We can have them. Can we borrow cups of fire from the sun? We can and must and light the world." --"On the Shoulders of Giants", Ray Bradbury

All of my posts are full of fiction and blatant lies.


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OfflineJust_a_Shadow
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Re: Autoclavable bag [Re: Sev]
    #2852554 - 07/02/04 10:38 PM (17 years, 4 months ago)

See what I had in mind was, Take the bag, fill it with brf/verm, any space is aired out so the bag is flat, then tied with something, so when its being PC'ed the bag wont expand all that much. So then take it out, wait awhile to make sure theres no contams, then inoculate it, cover hole with tape, shake it all up. Would this work?


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OfflineGr0wer
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Re: Autoclavable bag [Re: Just_a_Shadow]
    #2852794 - 07/03/04 01:05 AM (17 years, 4 months ago)

You need an impulse sealer and a glovebox or flowhood. You could hot glue the opening shut though in your glovebox. And you dont seal it until after pc'ing. You could hot glue a soda bottle top onto the opening after filling with grain and put a polyfill wad in the hold and foil that. The tapeing works.


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OfflineJust_a_Shadow
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Re: Autoclavable bag [Re: Gr0wer]
    #2853570 - 07/03/04 10:35 AM (17 years, 4 months ago)

what garin would be best for this?


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Offlinebillbraski
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Re: Autoclavable bag [Re: Just_a_Shadow]
    #2853683 - 07/03/04 12:13 PM (17 years, 4 months ago)

I say stick with your original idea, brf + verm, then use a makeshift collar(soda pop bottle top) and then stuff with cotton or polyfill.
grain is susceptible to sour-rot, so you have a better chance of success with brf+verm.


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OfflineJust_a_Shadow
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Re: Autoclavable bag [Re: billbraski]
    #2853933 - 07/03/04 02:22 PM (17 years, 4 months ago)

Ok, thanks for the ideas and comments, shroom on  :mushroom2::grin:


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OfflineGr0wer
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Re: Autoclavable bag [Re: Just_a_Shadow]
    #2854020 - 07/03/04 02:59 PM (17 years, 4 months ago)

That is true but grain is cheaper and a better substrate and spawn.


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OfflinepsilocyberV
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Re: Autoclavable bag [Re: Just_a_Shadow]
    #2855975 - 07/04/04 12:07 PM (17 years, 4 months ago)

Since you are using the pressure cooker you shouldn't have any problem sterilizing grains properly. It's also entirely possible to seal bags without an impulse sealer or a flow hood. Even using those cheap handheld "euro sealer" deals found at Wal-Mart for $20 is OK. Just make sure you seal the bag after it has a chance to cool inside the cooker and seal it as soon as it is removed from the cooker in as sterile setting possible (very clean work area with liberal use of a spray disenfectant).

Here is a very good illustrated tek for using autoclave bags to sterilize rye grass seed, just substitute whatever grain or substrate (BFR/Verm if you wish) and follow these basic steps.

http://www.sporeworks.com/labnotes/ryebagtek.html


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