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Invisiblemind.at.large
Myconerd


Registered: 12/13/16
Posts: 1,218
Loc: Floating in liquid gardens
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Vast218]
    #28517988 - 10/25/23 05:40 PM (3 months, 20 days ago)

Damn Rev that’s crazy potent! I wonder if you could reach out to miraculix or whoever and see if they can give you the chart for stronger potencies


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OfflineThe Tao
Look at the flowers.


Registered: 09/12/19
Posts: 1,568
Loc: Canada
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ReverendMyc]
    #28518015 - 10/25/23 06:01 PM (3 months, 20 days ago)

Can you do a serial dilution on the Miraculix test?


Edited by The Tao (10/25/23 06:03 PM)


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OfflineA.k.aM
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: mind.at.large] * 4
    #28518026 - 10/25/23 06:08 PM (3 months, 20 days ago)

I was just cleaning out stacks of old plates piling up by my hood.

Guess this lc was a good one. I bet I’d catch a buzz from them, after 5+ months they should be pretty loaded. And somehow the caps look better than my tubs do.





Cloning time :dancer:


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LAGM2020


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,580
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: mind.at.large] * 2
    #28518284 - 10/25/23 09:21 PM (3 months, 20 days ago)

They have been contacted and confirmed that you can use half dilution and double the results for more potent materials (or other appropriate inverse ratios). The US based company, QTest, added it to the instructions. Probably in the Miraculix instructions now, too, but I haven't seen those myself.


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LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
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OfflineMycoactive
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Registered: 11/20/19
Posts: 185
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ReverendMyc] * 3
    #28518295 - 10/25/23 09:36 PM (3 months, 20 days ago)

I had a similar result with miraculix and reached out. They said the color was so dark they assumed I hadn't properly followed the instructions and had "overcooked" the material. Anyways, I think there might be additional alkaloids present in the mushrooms that interfere with the results from that particular test because even though they are certainly potent, they tend to test around 2% (20 mg/g) with HPLC analysis, and based on experiences (1 g being quite profound), ~20 mg/g feels about right.


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Invisibleelpico
Stranger

Registered: 11/28/16
Posts: 321
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Mycoactive] * 9
    #28518378 - 10/26/23 12:01 AM (3 months, 20 days ago)

First crack at the wild Xico print.  This cased tray sat for 3.5 months doing nothing, so I scraped the casing, dunked and re-cased.  It's now throwing out sporadic fruits that grow in every direction.  The mature caps have a nice wild-type appearance and stay convex, without curling up.  Got a lot of work to do, but I'm pleased to have my first ever subtropicales fruits!



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OfflinePsiloPsychIn
PsiloPsychIn
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Registered: 06/17/14
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: elpico]
    #28518460 - 10/26/23 02:49 AM (3 months, 20 days ago)

Nice work!
Always rewarding to see a new set of wild genetics fruiting. 😀


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What are they saying? Listen carefully, it might be something you need to hear...





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Invisibleekabpils
Exploring Exotics
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Registered: 10/20/21
Posts: 181
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: elpico] * 16
    #28518462 - 10/26/23 02:52 AM (3 months, 20 days ago)

Another genetics of Subtropicalis. Substrate is pasteurized coir, straw, hay, manure, beech and pine shavings. Long and thick fruit bodies.



The same genetics on coir only substrate - short and thin:


And the same on coir + straw + manure - short but thick:


As you see it depends a lot on substrate nutrition!


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Edited by ekabpils (10/26/23 02:57 AM)


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OfflinePsiloPsychIn
PsiloPsychIn
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ekabpils] * 1
    #28518464 - 10/26/23 02:59 AM (3 months, 20 days ago)

Wow
Those are amazing flushes!
Thank you for sharing your discoveries regarding substrate!


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What are they saying? Listen carefully, it might be something you need to hear...





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Offlinechris77
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Registered: 01/17/22
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ekabpils] * 2
    #28518466 - 10/26/23 03:05 AM (3 months, 20 days ago)

great stuff ekabpils! looking good

yes, many contributing factors.

i have an observation to share.
on my recent run i got tons of spaghetti growing towards the light source (window), looking all curly and twisted.

as soon as i have allowed for some more FAE, they began to grow straight up. like absolutely straight. not the slightest inclination towards the light remained.
i didnt change the position of the light or anything else. just some more FAE

which leads me to the idea, that, when lacking Air they grow towards the light (which sounds like a reasonable and natural impulse/reaponse) but when there is enough air they will grow against the gravitational pull and ignore the light source, and in doing so maximize the spread of spores.

i will put up two pics showing this when i cut them in a few days


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the observer is the observed
            j. krishnamurti


Edited by chris77 (10/26/23 03:33 AM)


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OfflineDERRAYLD
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Registered: 05/13/02
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: PsiloPsychIn] * 2
    #28518467 - 10/26/23 03:06 AM (3 months, 20 days ago)

I'm going to run some tests using horse dung/coir substrate as well.
Abundance of cheap manure available.


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Invisibleekabpils
Exploring Exotics
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Registered: 10/20/21
Posts: 181
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: DERRAYLD] * 3
    #28518473 - 10/26/23 03:24 AM (3 months, 20 days ago)

Yes chris77 - in all the cases I do a lot of FAE. If FAE is low they will go spagetti style.


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OfflineA.k.aM
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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ekabpils] * 1
    #28518511 - 10/26/23 05:09 AM (3 months, 20 days ago)

Quote:

ekabpils said:
Another genetics of Subtropicalis. Substrate is pasteurized coir, straw, hay, manure, beech and pine shavings. Long and thick fruit bodies.



The same genetics on coir only substrate - short and thin:


And the same on coir + straw + manure - short but thick:


As you see it depends a lot on substrate nutrition!






This was all the same culture? Or multiple runs from one print?


They look so much better with the nice big caps.


--------------------
LAGM2020


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OfflineVast218
Learning
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Registered: 04/24/22
Posts: 123
Last seen: 1 month, 20 days
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: elpico] * 1
    #28518518 - 10/26/23 05:29 AM (3 months, 20 days ago)

Quote:

elpico said:
First crack at the wild Xico print.  This cased tray sat for 3.5 months doing nothing, so I scraped the casing, dunked and re-cased.  It's now throwing out sporadic fruits that grow in every direction.  The mature caps have a nice wild-type appearance and stay convex, without curling up.  Got a lot of work to do, but I'm pleased to have my first ever subtropicales fruits!






That a good start and the no upturned cap is interesting. If these can just be made to fruit consistently you may soon have a whole different pheno.


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Invisibleekabpils
Exploring Exotics
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Registered: 10/20/21
Posts: 181
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: A.k.a] * 1
    #28518525 - 10/26/23 05:46 AM (3 months, 20 days ago)

Quote:

This was all the same culture? Or multiple runs from one print?


They look so much better with the nice big caps.




From the same isolated culture on agar.


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InvisibleSwabMarleyS
Twisted Metal Head
Male


Registered: 12/07/20
Posts: 1,553
Loc: Drunken Stupor
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: ekabpils] * 1
    #28518533 - 10/26/23 06:04 AM (3 months, 20 days ago)

Interesting work mate! Seems they like a bit of HP then among other things!


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InvisibleYuggoth
Mi-Go Cultivator
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Registered: 03/04/23
Posts: 496
Loc: Lost Carcosa
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: SwabMarley]
    #28518782 - 10/26/23 11:01 AM (3 months, 20 days ago)

Do our official vendors have good spores for these guys or is everyone trading?


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We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

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Offlinetryptkaloids
Learner
I'm a teapot


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Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Yuggoth]
    #28518794 - 10/26/23 11:17 AM (3 months, 20 days ago)

Check SW


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,580
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Yuggoth] * 7
    #28518798 - 10/26/23 11:18 AM (3 months, 20 days ago)

I know a guy::pm:

Second round turned out more prettier.


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LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic




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OfflineBustaboo
Calmer than you are


Registered: 07/17/23
Posts: 118
Last seen: 1 month, 2 hours
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Yuggoth] * 2
    #28518800 - 10/26/23 11:18 AM (3 months, 20 days ago)

I just ordered a 4 pack of exotics from a vendor. Started a semp germ plate 2 days ago, but nothing yet.


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Looking for Yeti and Ghost spores.


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