|
bongoman
Stranger

Registered: 09/19/18
Posts: 558
Last seen: 24 days, 20 hours
|
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri]
#28469167 - 09/14/23 07:37 PM (4 months, 30 days ago) |
|
|
Thanks konbri, very helpful.
|
JW123
Nooby Nooby Noob


Registered: 01/17/22
Posts: 1,325
Loc: Tn
Last seen: 1 hour, 3 minutes
|
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: nosf3r4tu] 5
#28471357 - 09/16/23 04:37 PM (4 months, 29 days ago) |
|
|
Trying these out tonight.

|
ReverendMyc

Registered: 03/29/19
Posts: 1,580
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc] 9
#28477176 - 09/20/23 09:04 PM (4 months, 24 days ago) |
|
|
A follow up on the myceliated grain age vs potency test.
  
If all 4 jars stay clean, I will test potency at 3, 6, 9 and 12 months. Hopefully one will make it to 12 months and I will definitely test one at 3 months. So that should at least give us some data to support or deny potency increase with time. Only 352 days until we find out. Someone send me a reminder next September.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
|
Melgo
Semperviva Fanatic



Registered: 02/11/16
Posts: 698
Loc: Western Europe
Last seen: 1 hour, 39 minutes
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc] 2
#28477291 - 09/21/23 12:55 AM (4 months, 24 days ago) |
|
|
Quote:
ReverendMyc said: A follow up on the myceliated grain age vs potency test.
  
If all 4 jars stay clean, I will test potency at 3, 6, 9 and 12 months. Hopefully one will make it to 12 months and I will definitely test one at 3 months. So that should at least give us some data to support or deny potency increase with time. Only 352 days until we find out. Someone send me a reminder next September.
Yeah man! Awesome tests
|
chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,930
Last seen: 58 minutes, 41 seconds
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: Melgo] 1
#28477306 - 09/21/23 02:53 AM (4 months, 24 days ago) |
|
|
-------------------- the observer is the observed j. krishnamurti
|
RockinRobot
Stranger


Registered: 12/08/22
Posts: 898
Last seen: 7 minutes, 38 seconds
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: chris77]
#28477617 - 09/21/23 12:23 PM (4 months, 24 days ago) |
|
|
Well getting ready to join in on the fun. Semp print arrived in the mail today going to agar tonight or tomorrow.
Anything special I need to know? From reading this thread seems they grow just like Cubes. Planning to run in purple handled 6qt shoeboxes.
|
Telomere
Unravelling


Registered: 12/26/22
Posts: 185
Last seen: 31 minutes, 41 seconds
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: RockinRobot] 2
#28477620 - 09/21/23 12:29 PM (4 months, 24 days ago) |
|
|
Quote:
RockinRobot said: Well getting ready to join in on the fun. Semp print arrived in the mail today going to agar tonight or tomorrow.
Anything special I need to know? From reading this thread seems they grow just like Cubes. Planning to run in purple handled 6qt shoeboxes.
In my experience they need a bit more humidity, especially at pin stage and onwards. I tried to grow them exactly like cubes in a shoebox and they aborted while pinning. Added a pretty thick coir casing layer and they did better. Put the shoebox inside a monotub and with an air stone and single fogger disk on a timer and they started to perform great. Ended up with about 25g dried from a single quart of spawn.
Edited by Telomere (09/21/23 01:18 PM)
|
RockinRobot
Stranger


Registered: 12/08/22
Posts: 898
Last seen: 7 minutes, 38 seconds
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: Telomere]
#28477622 - 09/21/23 12:31 PM (4 months, 24 days ago) |
|
|
Quote:
Telomere said:
Quote:
RockinRobot said: Well getting ready to join in on the fun. Semp print arrived in the mail today going to agar tonight or tomorrow.
Anything special I need to know? From reading this thread seems they grow just like Cubes. Planning to run in purple handled 6qt shoeboxes.
In my experience they need a bit more atmospheric humidity, especially at pin stage. I tried to grow them exactly like cubes in a shoebox and they aborted while pinning. Added a pretty thick coir casing layer and they did better. Put the shoebox inside a monotub and with an air stone and single fogger disk on a timer and they started to perform great. Ended up with about 25g dried from a single quart of spawn.
Think they would work better in my Martha?
|
chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,930
Last seen: 58 minutes, 41 seconds
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: RockinRobot]
#28477642 - 09/21/23 01:02 PM (4 months, 24 days ago) |
|
|
they love it wet once colonisation is done...
-------------------- the observer is the observed j. krishnamurti
|
Telomere
Unravelling


Registered: 12/26/22
Posts: 185
Last seen: 31 minutes, 41 seconds
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: chris77]
#28477654 - 09/21/23 01:16 PM (4 months, 24 days ago) |
|
|
Have you been able to try them out yet Chris?
|
ReverendMyc

Registered: 03/29/19
Posts: 1,580
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: chris77] 5
#28477655 - 09/21/23 01:16 PM (4 months, 24 days ago) |
|
|
I would add that I have been most successful starting with under field capacity coir, THEN keep it moist once the sub is well colonized. Dry early and damp later, ime.
EDIT to add example pictures:
  These were spawned 6 days ago and about ready for a mist. The top layer on the sparser one is about twice as thick as the more colonized one, but I am happy with both right now.
Edited by ReverendMyc (09/21/23 01:29 PM)
|
psilocybist
All is One



Registered: 05/22/14
Posts: 531
Loc: Far
Last seen: 4 days, 5 hours
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc]
#28477748 - 09/21/23 02:54 PM (4 months, 24 days ago) |
|
|
Quote:
ReverendMyc said: A follow up on the myceliated grain age vs potency test.
  
If all 4 jars stay clean, I will test potency at 3, 6, 9 and 12 months. Hopefully one will make it to 12 months and I will definitely test one at 3 months. So that should at least give us some data to support or deny potency increase with time. Only 352 days until we find out. Someone send me a reminder next September.
This is a great great examination. Thanks for doing it and thanks in advance for reporting the results back. But it would be much much better if done based on a more frequented sampling schedule. So that a much proper and precise alkaloid/time curve could be graphed. This graph would help tremendously in a try to find a sweet-time-spot for aging myceliated grains. (i.e. finding a time-range that it's not efficient anymore to wait, but also before that period, harvesting/extraction is too soon to be efficient)
 If you consider helping finding such curve with valid/useful data, the sampling rate should be more frequent for a while, then more sparse at larger timespans. Something like this suggestion: 0 days (that's @ full colonization) 7~8 days 14~16 days 28~32 days 50~55 days 75~80 days 150~170 days 280-290 days 365 days
That's 9 checkpoints, maybe a little too crowded. But I'm sure you get the idea on how to make it a little sparser while still collecting data at more useful times.
Hopefully we see a plot which looks like the green one, or at least the middle line, but definitely not like the red one.
|
ReverendMyc

Registered: 03/29/19
Posts: 1,580
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: psilocybist] 2
#28477828 - 09/21/23 04:21 PM (4 months, 24 days ago) |
|
|
I like your line of reasoning. My initial intent was just to confirm or deny that potency increases with time, which is still not known. If not, this whole line of inquiry would be moot.
On the other hand, with the time line involved, it would kind of suck to to wait a year or even 6 months to determine if your line is worth a follow up experiment.
Since I still have about 100 ml of the li that these jars started from and millet is cheap. I think I will go ahead and start additional jars. That will give me some room for any potential failures. It also lets me get a test on the 0 and 7 days of full colonization since those have already passed for the first set. The only significant loss would be the cost of tests, which is not insurmountable.
It also just occurred to me that I can dry a much smaller sample size and spawn the rest for fruits so nothing is really wasted in the early stages where we assume that the dried myceliated grains will be very weak.
 Remaining li from the previous batch.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
|
ReverendMyc

Registered: 03/29/19
Posts: 1,580
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc] 6
#28478867 - 09/22/23 03:51 PM (4 months, 23 days ago) |
|
|
Double post to note that I started a thread to document the progress of this experiment. Feel free to make critiques or suggestions.
Myceliated Grain Spawn Potency vs Time test
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
|
Bigworm



Registered: 03/19/22
Posts: 1,902
Loc: Murica
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc]
#28483221 - 09/25/23 10:49 PM (4 months, 19 days ago) |
|
|
I spawned this mono back on 5/30 from a clone. The clone was the biggest fruit in a big cluster, only took 3 weeks to pin. I waited almost 3 months for this to start pinning this time. (I'm very disappointed in the performance of this culture and I am currently awaiting some spores to geminate on a few plates.) The biggest fruit is almost a month old and still hasn't darkened and I don't see any spores dropping or forming. Any thoughts? Should I just harvest what I have or let them go?
|
rockyfungus
dirty


Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 12 days, 7 hours
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: Bigworm]
#28483232 - 09/25/23 11:19 PM (4 months, 19 days ago) |
|
|
Wild, I did so much better early on with these. I've had 3 diff cultures fail to take to grain now. Time to recheck my process and figure out what's happened.
Cubes, mexicana, pans, and gourmets are taking but semps are noping out. Agar doesn't even try reach into grain. This current run was 1/5 failure and it was always semp.
Like I get things failing at a shake but it's like an inability for them to eat.
|
RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,756
Loc: State of Confusion
Last seen: 6 hours, 12 minutes
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: rockyfungus] 1
#28483298 - 09/26/23 04:18 AM (4 months, 19 days ago) |
|
|
I’ve anecdotally noticed better performance from my MS grows than from any cloning I try to do. The longer I hold on to a culture the worse it does. I’ve now gone back to spores to see if the trend continues. If it does, given the short shelf life of spores, these will have to be in continuous rotation for me. Storage of a culture may be problematic.
|
SwabMarley
Twisted Metal Head



Registered: 12/07/20
Posts: 1,553
Loc: Drunken Stupor
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: RoscoeReturns] 2
#28483323 - 09/26/23 05:29 AM (4 months, 19 days ago) |
|
|
Quote:
RoscoeReturns said: I’ve anecdotally noticed better performance from my MS grows than from any cloning I try to do. The longer I hold on to a culture the worse it does. I’ve now gone back to spores to see if the trend continues. If it does, given the short shelf life of spores, these will have to be in continuous rotation for me. Storage of a culture may be problematic.
A always tend to get fairly decent grows from MS. More often than not an LC made from a germ plate too.
|
astra
Mycelia-Of-House-Fungi


Registered: 12/24/21
Posts: 81
Loc: Japan
Last seen: 4 days, 15 hours
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: RoscoeReturns]
#28483326 - 09/26/23 05:32 AM (4 months, 19 days ago) |
|
|
Quote:
RoscoeReturns said: I’ve anecdotally noticed better performance from my MS grows than from any cloning I try to do. The longer I hold on to a culture the worse it does. I’ve now gone back to spores to see if the trend continues. If it does, given the short shelf life of spores, these will have to be in continuous rotation for me. Storage of a culture may be problematic.
|
chris77
Archaic Revivalist



Registered: 01/17/22
Posts: 1,930
Last seen: 58 minutes, 41 seconds
|
Re: Official Ps. Subtropical/Semperviva Thread [Re: astra]
#28483343 - 09/26/23 05:59 AM (4 months, 19 days ago) |
|
|
well, then i can only hope that it's culture-dependent. got 3 boxes from 3 different clones working their way through the casings as we speak.. love their pace. they certainly got their own way. in a week i will finally get to try them
-------------------- the observer is the observed j. krishnamurti
|
|