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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,271
Last seen: 8 hours, 57 minutes
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How to get your finds DNA barcoded 6
#28479935 - 09/23/23 12:34 PM (4 months, 2 days ago) |
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ITS DNA barcoding is revolutionizing mushroom identification. It doesn't always tell you exactly what you have found, but it does tell you which other finds are probably the same species. When the name of species isn't known, a provisional name is assigned, so at least we have a name for everything.
Here is an example of a Hygrocybe which was sequenced - it is probably a new species, but it is possible that an old name applies and will be associated with this barcode in the future. It has been given the code name Hygrocybe "sp-IN10", which means that it was the tenth unknown Hygrocybe sequenced in Indiana. This one was found in Florida.
You can search for all of the observations with a certain code name with a URL like this: https://www.inaturalist.org/observations?verifiable=any&place_id=any&field:Provisional%20Species%20Name=Hygrocybe%20%22sp-IN10%22
You can search iNaturalist for DNA barcoded fungi with the following URL: https://www.inaturalist.org/observations?field:DNA%20Barcode%20ITS=
I use this all the time when I want to see photos of a certain species without all the misidentifications. For example Pluteus hongoi is common in the eastern USA, but is often misidentified for Pluteus cervinus.
Here are the DNA verified observations of Pluteus hongoi:
https://www.inaturalist.org/observations?place_id=any&subview=map&taxon_id=516992&field:DNA%20Barcode%20ITS=
It is now possible to get all of your finds DNA barcoded for free! They can be from anywhere in the world, you just need an iNaturalist observation #.

Collecting mushrooms and getting them barcoded is a really great way to add to the knowledge of which species exist, and to figure out what you are finding.
Stephen Russell has a goal of sequencing 100,000 mushroom collections from North America in the next ten years - when this is done we will have a much better idea of the fungal biodiversity that exists, and all the sequence data gets added to Genbank so it is available for free to everyone forever.
ITS sequencing is really good at species discrimination, but it doesn't give much insight into strains, so don't just send in your cubes. Full genome sequencing is good at this - for more information on that see https://entheome.org.
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,271
Last seen: 8 hours, 57 minutes
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