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InvisibleTexastransplant
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Registered: 09/15/22
Posts: 115
Restarting Colony issue
    #28479495 - 09/23/23 06:12 AM (1 year, 3 months ago)

I am having issues trying to restart several colonies. I took some clone samples (several Months back) of some strains I had cultivated from MSS -> Agar -> Grain.

I allowed the clone colony to get cleaned up. Several Transfers took place, and I stored a semi colonized plate in my fridge (double wrapped with saran wrap, and in a cooler). I took a transfer of the leading edges of these plates and restarted on a fresh agar plate. Everything looked nice, went to grain with the newly revived colony. Again, everything went well and looked normal.

After going to bulk, the tubs colonize like I would expect, however, that is where it stops. The below image is from today, 16 day's post bulk (other strains that were newer than this one have already pinned and I have harvested the first flush).

Its not terribly inconvenient if this one is another week out, but am I spinning my wheels on these? I had left the previous bulks sitting for 2 months with no fruit ever coming. I am assuming my colonies have basically died or are incapable of fruiting after sitting on a plate for extended periods of time, regardless of transferring and then going to bulk?

I have prepped several long term storage slants (using hamloafs method https://www.shroomery.org/forums/showflat.php/Number/25018245/fpart/1/vc/1), I have not ventured to restart from these, I will possibly give it a try, but wanted to reach out to those more experienced than me to see if I am just overlooking something.

I appreciate any guidance the group has.




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InvisiblefahtsterM
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Registered: 06/17/06
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Re: Restarting Colony issue [Re: Texastransplant]
    #28479517 - 09/23/23 06:36 AM (1 year, 3 months ago)

What variety is it? 

Is this the same culture that you had sitting for 2 months that never fruited?  If that’s the case, I’d chalk it up to a poor culture and trash it and go back to spore.

But as you say, 16 days isn’t all that long to wait for pinning.  I’ve had peu genes that took well over a month to show pins.  Most of the PE cultures I’ve ran take over 3 weeks to start knotting up

The amount of time on a plate shouldn’t matter.. more likely is, you further isolated the genes from the clone down to a poor fruit genetic line

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InvisibleTexastransplant
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Registered: 09/15/22
Posts: 115
Re: Restarting Colony issue [Re: fahtster]
    #28479524 - 09/23/23 06:44 AM (1 year, 3 months ago)

The current strain in question is B+. The last several ones were APE and PE that ended up as duds.

The PE was certainly not a bad colony, as I had grew several bulks from the colony I had stored and attempted to revive, but nothing came from it. I had clocked the PE colony at exactly 10 days from bulk to first pin in the previous runs, however, this last time it did nothing besides colonize the bin for 2 months. The substrate did shrink, but nothing ever fruited.

I had tried to take a shortcut with the previous ones, just using the fully colonized plate that was stored instead of transferring leading edge and starting fresh. This time with the B+ I took the extra step and time.

I do agree 16 days is short, especially since I know this one is a bit slower from my own personnel experience. Certainly dont want to waste time, energy and a bin on something that wont produce.

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InvisiblefahtsterM
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Re: Restarting Colony issue [Re: Texastransplant]
    #28479536 - 09/23/23 06:53 AM (1 year, 3 months ago)

Yeah I’d just give it more time then.  After looking closer at the pics, it looks like it’s about to start knotting up to me

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InvisibleTexastransplant
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Registered: 09/15/22
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Re: Restarting Colony issue [Re: fahtster]
    #28479543 - 09/23/23 06:57 AM (1 year, 3 months ago)

I did notice that yesterday around the edges. I was certain the other 2 dud grows showed the same.

Will let it sit and do its thing then.

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