Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate

Jump to first unread post Pages: < First | < Back | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 | 181 | 182 | 183 | 184 | 185 | 186 | 187 | 188 | 189 | 190 | Next > | Last >
Offlinebongoman
Stranger
Male User Gallery
Registered: 09/19/18
Posts: 558
Last seen: 24 days, 20 hours
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: Konbri]
    #28469167 - 09/14/23 07:37 PM (4 months, 30 days ago)

Thanks konbri, very helpful.


Extras: Filter Print Post Top
OfflineJW123
Nooby Nooby Noob
 User Gallery

Registered: 01/17/22
Posts: 1,325
Loc: Tn
Last seen: 1 hour, 3 minutes
Re: Official Ps. Subtropicalis/Semperviva Thread [Re: nosf3r4tu] * 5
    #28471357 - 09/16/23 04:37 PM (4 months, 29 days ago)

Trying these out tonight.

:excited:


Extras: Filter Print Post Top
InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,580
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc] * 9
    #28477176 - 09/20/23 09:04 PM (4 months, 24 days ago)

A follow up on the myceliated grain age vs potency test.


If all 4 jars stay clean, I will test potency at 3, 6, 9 and 12 months. Hopefully one will make it to 12 months and I will definitely test one at 3 months. So that should at least give us some data to support or deny potency increase with time. Only 352 days until we find out. Someone send me a reminder next September.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic




Extras: Filter Print Post Top
OfflineMelgo
Semperviva Fanatic
Male User Gallery


Registered: 02/11/16
Posts: 698
Loc: Western Europe
Last seen: 1 hour, 39 minutes
Trusted Cultivator
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc] * 2
    #28477291 - 09/21/23 12:55 AM (4 months, 24 days ago)

Quote:

ReverendMyc said:
A follow up on the myceliated grain age vs potency test.


If all 4 jars stay clean, I will test potency at 3, 6, 9 and 12 months. Hopefully one will make it to 12 months and I will definitely test one at 3 months. So that should at least give us some data to support or deny potency increase with time. Only 352 days until we find out. Someone send me a reminder next September.




Yeah man! Awesome tests :rockon:


Extras: Filter Print Post Top
Offlinechris77
Archaic Revivalist
 User Gallery


Registered: 01/17/22
Posts: 1,930
Last seen: 58 minutes, 47 seconds
Re: Official Ps. Subtropical/Semperviva Thread [Re: Melgo] * 1
    #28477306 - 09/21/23 02:53 AM (4 months, 24 days ago)

:whathesaid::havesomescience:
:aweyeah:


--------------------
the observer is the observed
            j. krishnamurti


Extras: Filter Print Post Top
OnlineRockinRobot
Stranger


Registered: 12/08/22
Posts: 898
Last seen: 7 minutes, 44 seconds
Re: Official Ps. Subtropical/Semperviva Thread [Re: chris77]
    #28477617 - 09/21/23 12:23 PM (4 months, 24 days ago)

Well getting ready to join in on the fun. Semp print arrived in the mail today going to agar tonight or tomorrow.

Anything special I need to know? From reading this thread seems they grow just like Cubes. Planning to run in purple handled 6qt shoeboxes.


Extras: Filter Print Post Top
OfflineTelomere
Unravelling
 User Gallery

Registered: 12/26/22
Posts: 185
Last seen: 31 minutes, 47 seconds
Re: Official Ps. Subtropical/Semperviva Thread [Re: RockinRobot] * 2
    #28477620 - 09/21/23 12:29 PM (4 months, 24 days ago)

Quote:

RockinRobot said:
Well getting ready to join in on the fun. Semp print arrived in the mail today going to agar tonight or tomorrow.

Anything special I need to know? From reading this thread seems they grow just like Cubes. Planning to run in purple handled 6qt shoeboxes.




In my experience they need a bit more humidity, especially at pin stage and onwards. I tried to grow them exactly like cubes in a shoebox and they aborted while pinning. Added a pretty thick coir casing layer and they did better. Put the shoebox inside a monotub and with an air stone and single fogger disk on a timer and they started to perform great. Ended up with about 25g dried from a single quart of spawn.


--------------------



Edited by Telomere (09/21/23 01:18 PM)


Extras: Filter Print Post Top
OnlineRockinRobot
Stranger


Registered: 12/08/22
Posts: 898
Last seen: 7 minutes, 44 seconds
Re: Official Ps. Subtropical/Semperviva Thread [Re: Telomere]
    #28477622 - 09/21/23 12:31 PM (4 months, 24 days ago)

Quote:

Telomere said:
Quote:

RockinRobot said:
Well getting ready to join in on the fun. Semp print arrived in the mail today going to agar tonight or tomorrow.

Anything special I need to know? From reading this thread seems they grow just like Cubes. Planning to run in purple handled 6qt shoeboxes.




In my experience they need a bit more atmospheric humidity, especially at pin stage. I tried to grow them exactly like cubes in a shoebox and they aborted while pinning. Added a pretty thick coir casing layer and they did better. Put the shoebox inside a monotub and with an air stone and single fogger disk on a timer and they started to perform great. Ended up with about 25g dried from a single quart of spawn.




Think they would work better in my Martha?


Extras: Filter Print Post Top
Offlinechris77
Archaic Revivalist
 User Gallery


Registered: 01/17/22
Posts: 1,930
Last seen: 58 minutes, 47 seconds
Re: Official Ps. Subtropical/Semperviva Thread [Re: RockinRobot]
    #28477642 - 09/21/23 01:02 PM (4 months, 24 days ago)

they love it wet once colonisation is done...


--------------------
the observer is the observed
            j. krishnamurti


Extras: Filter Print Post Top
OfflineTelomere
Unravelling
 User Gallery

Registered: 12/26/22
Posts: 185
Last seen: 31 minutes, 47 seconds
Re: Official Ps. Subtropical/Semperviva Thread [Re: chris77]
    #28477654 - 09/21/23 01:16 PM (4 months, 24 days ago)

Have you been able to try them out yet Chris?


--------------------



Extras: Filter Print Post Top
InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,580
Re: Official Ps. Subtropical/Semperviva Thread [Re: chris77] * 5
    #28477655 - 09/21/23 01:16 PM (4 months, 24 days ago)

I would add that I have been most successful starting with under field capacity coir, THEN keep it moist once the sub is well colonized. Dry early and damp later, ime.

EDIT to add example pictures:

These were spawned 6 days ago and about ready for a mist. The top layer on the sparser one is about twice as thick as the more colonized one, but I am happy with both right now.


Edited by ReverendMyc (09/21/23 01:29 PM)


Extras: Filter Print Post Top
Offlinepsilocybist
All is One
Male User Gallery


Registered: 05/22/14
Posts: 531
Loc: Far
Last seen: 4 days, 5 hours
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc]
    #28477748 - 09/21/23 02:54 PM (4 months, 24 days ago)

Quote:

ReverendMyc said:
A follow up on the myceliated grain age vs potency test.


If all 4 jars stay clean, I will test potency at 3, 6, 9 and 12 months. Hopefully one will make it to 12 months and I will definitely test one at 3 months. So that should at least give us some data to support or deny potency increase with time. Only 352 days until we find out. Someone send me a reminder next September.




This is a great great examination.
Thanks for doing it and thanks in advance for reporting the results back.
But it would be much much better if done based on a more frequented sampling schedule.
So that a much proper and precise alkaloid/time curve could be graphed.
This graph would help tremendously in a try to find a sweet-time-spot for aging myceliated grains. (i.e. finding a time-range that it's not efficient anymore to wait, but also before that period, harvesting/extraction is too soon to be efficient)


If you consider helping finding such curve with valid/useful data, the sampling rate should be more frequent for a while, then more sparse at larger timespans.
Something like this suggestion:
0 days (that's @ full colonization)
7~8 days
14~16 days
28~32 days
50~55 days
75~80 days
150~170 days
280-290 days
365 days

That's 9 checkpoints, maybe a little too crowded.
But I'm sure you get the idea on how to make it a little sparser while still collecting data at more useful times.

Hopefully we see a plot which looks like the green one, or at least the middle line, but definitely not like the red one.


Extras: Filter Print Post Top
InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,580
Re: Official Ps. Subtropical/Semperviva Thread [Re: psilocybist] * 2
    #28477828 - 09/21/23 04:21 PM (4 months, 24 days ago)

I like your line of reasoning. My initial intent was just to confirm or deny that potency increases with time, which is still not known. If not, this whole line of inquiry would be moot.

On the other hand, with the time line involved, it would kind of suck to to wait a year or even 6 months to determine if your line is worth a follow up experiment.

Since I still have about 100 ml of the li that these jars started from and millet is cheap. I think I will go ahead and start additional jars. That will give me some room for any potential failures. It also lets me get a test on the 0 and 7 days of full colonization since those have already passed for the first set. The only significant loss would be the cost of tests, which is not insurmountable.

It also just occurred to me that I can dry a much smaller sample size and spawn the rest for fruits so nothing is really wasted in the early stages where we assume that the dried myceliated grains will be very weak.


Remaining li from the previous batch.




--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic




Extras: Filter Print Post Top
InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,580
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc] * 6
    #28478867 - 09/22/23 03:51 PM (4 months, 23 days ago)

Double post to note that I started a thread to document the progress of this experiment. Feel free to make critiques or suggestions.

Myceliated Grain Spawn Potency vs Time test


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023
Don't Panic




Extras: Filter Print Post Top
InvisibleBigwormS
Male User Gallery


Registered: 03/19/22
Posts: 1,902
Loc: Murica
Re: Official Ps. Subtropical/Semperviva Thread [Re: ReverendMyc]
    #28483221 - 09/25/23 10:49 PM (4 months, 19 days ago)

I spawned this mono back on 5/30 from a clone. The clone was the biggest fruit in a big cluster, only took 3 weeks to pin. I waited almost 3 months for this to start pinning this time.
(I'm very disappointed in the performance of this culture and I am currently awaiting some spores to geminate on a few plates.) The biggest fruit is almost a month old and still hasn't darkened and I don't see any spores dropping or forming. Any thoughts? Should I just harvest what I have or let them go?


Extras: Filter Print Post Top
Offlinerockyfungus
dirty
 User Gallery

Registered: 03/01/21
Posts: 1,062
Loc: Front range
Last seen: 12 days, 7 hours
Re: Official Ps. Subtropical/Semperviva Thread [Re: Bigworm]
    #28483232 - 09/25/23 11:19 PM (4 months, 19 days ago)

Wild, I did so much better early on with these. I've had 3 diff cultures fail to take to grain now. Time to recheck my process and figure out what's happened.

Cubes, mexicana, pans, and gourmets are taking but semps are noping out. Agar doesn't even try reach into grain. This current run was 1/5 failure and it was always semp.

Like I get things failing at a shake but it's like an inability for them to eat.


Extras: Filter Print Post Top
OfflineRoscoeReturnsS
Crotchety chode man
Male


Registered: 02/12/18
Posts: 1,756
Loc: State of Confusion
Last seen: 6 hours, 12 minutes
Re: Official Ps. Subtropical/Semperviva Thread [Re: rockyfungus] * 1
    #28483298 - 09/26/23 04:18 AM (4 months, 19 days ago)

I’ve anecdotally noticed better performance from my MS grows than from any cloning I try to do. The longer I hold on to a culture the worse it does. I’ve now gone back to spores to see if the trend continues. If it does, given the short shelf life of spores, these will have to be in continuous rotation for me. Storage of a culture may be problematic.


Extras: Filter Print Post Top
InvisibleSwabMarleyS
Twisted Metal Head
Male


Registered: 12/07/20
Posts: 1,553
Loc: Drunken Stupor
Re: Official Ps. Subtropical/Semperviva Thread [Re: RoscoeReturns] * 2
    #28483323 - 09/26/23 05:29 AM (4 months, 19 days ago)

Quote:

RoscoeReturns said:
I’ve anecdotally noticed better performance from my MS grows than from any cloning I try to do. The longer I hold on to a culture the worse it does. I’ve now gone back to spores to see if the trend continues. If it does, given the short shelf life of spores, these will have to be in continuous rotation for me. Storage of a culture may be problematic.



A always tend to get fairly decent grows from MS. More often than not an LC made from a germ plate too.


Extras: Filter Print Post Top
Offlineastra
Mycelia-Of-House-Fungi
Female

Registered: 12/24/21
Posts: 81
Loc: Japan
Last seen: 4 days, 16 hours
Re: Official Ps. Subtropical/Semperviva Thread [Re: RoscoeReturns]
    #28483326 - 09/26/23 05:32 AM (4 months, 19 days ago)

Quote:

RoscoeReturns said:
I’ve anecdotally noticed better performance from my MS grows than from any cloning I try to do. The longer I hold on to a culture the worse it does. I’ve now gone back to spores to see if the trend continues. If it does, given the short shelf life of spores, these will have to be in continuous rotation for me. Storage of a culture may be problematic.




:whathesaid:


--------------------
:ancientaliens:


Extras: Filter Print Post Top
Offlinechris77
Archaic Revivalist
 User Gallery


Registered: 01/17/22
Posts: 1,930
Last seen: 58 minutes, 47 seconds
Re: Official Ps. Subtropical/Semperviva Thread [Re: astra]
    #28483343 - 09/26/23 05:59 AM (4 months, 19 days ago)

well, then i can only hope that it's culture-dependent. got 3 boxes from 3 different clones working their way through the casings as we speak.. love their pace. they certainly got their own way. in a week i will finally get to try them :mindblown:


--------------------
the observer is the observed
            j. krishnamurti


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 170 | 171 | 172 | 173 | 174 | 175 | 176 | 177 | 178 | 179 | 180 | 181 | 182 | 183 | 184 | 185 | 186 | 187 | 188 | 189 | 190 | Next > | Last >

Shop: PhytoExtractum Buy Bali Kratom Powder   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* The OFFICIAL Stone producer/sclerotia thread and discussion
( 1 2 3 4 ... 650 651 )
firstTIMER420 470,626 13,001 02/16/24 03:02 PM
by tree frog
* Official Psilocybe zapotecorum thread
( 1 2 3 4 ... 25 26 )
Hindsight 17,884 512 02/16/24 04:57 PM
by inski
* Official Misc EXOTIC Thread
( 1 2 3 4 ... 139 140 )
Jakeoncid419 113,054 2,792 02/16/24 04:02 PM
by the_chosen_one
* Artificial lighting: what is best for p cubes? Elskiiworld 104 5 02/13/24 12:42 PM
by Beautation
* The Official Woodlovers Thread
( 1 2 3 4 ... 714 715 )
TheDuder 473,710 14,292 02/15/24 08:41 PM
by SingularFusion
* Help a girl out with making grain spawn for the first time - P. natalensis slow growth and light blue color on 1 jar (contam?) psilocybe-maria 253 9 02/15/24 04:06 PM
by psilocybe-maria
* Official Copelandia/Panaeolus Thread
( 1 2 3 4 ... 1604 1605 )
Fantastic Mr. Fox 930,605 32,090 02/16/24 09:27 AM
by tree frog
* Printing Semperviva Javadog 2,731 17 01/23/11 01:48 PM
by Javadog

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
143,810 topic views. 28 members, 186 guests and 44 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.054 seconds spending 0.029 seconds on 15 queries.