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InvisibleStriplessRED
Registered: 09/05/07
Posts: 562
Loc: Helena
F2 strategy
    #28477038 - 09/20/23 08:00 PM (4 months, 5 days ago)

HI everyone,

I am interested in crossing some mushroom strains, but I have a question regarding the F2 generation. I have read these posts, but I am unclear on what do to after the F1 fruit.

So if you cross two monokaryotes (nice 'Methods' section HERE) and then fruit the resulting dikaryotic myc, which of the following would give you the ideal F2 gen:

1. Take a single spore print of F1 -> germinate -> fruit
2. Clone a single F1 fruit
3. Take 2 spore prints (or clones) from different fruit and repeat the monokaryote isolation & mating process

I guess my question is, will a single hybridized fruit (ie AaBb) be able to cross with its own genetics or do you need a second fruit?

thanks in advance


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Invisiblecovertjoy

Registered: 07/09/23
Posts: 272
Re: F2 strategy [Re: StriplessRED]
    #28477320 - 09/21/23 04:00 AM (4 months, 4 days ago)

A spore print from a single fruit and regular germination will suffice.

If you have done mon-mon mating on agar then your F1 grow will be a pure culture and all your fruits in theory will have the same genotype (although fungi are weird so don't count on it). In which case a print from any single fruit makes sense. However if your cross was done with something like the Workman method you may have a 'multi-cross' grow in which case you should select the F1 from the most desirable fruit or even take multiple F1 lines.

When spores are created by the F1 fruit the genome is recombinated such that each spore has it's own unique haploid genome. When it comes to producing your F2 generation you can germinate spores from a single F1 fruit and yes they will mate with each other, producing a wide range of phenotypes.

Something like PF-Tek where you can inject multi-spore solution would probably be ideal rather than cleaning up a pure culture on agar, as you want to run as many F2 genotypes as possible.


--------------------


Edited by covertjoy (09/21/23 05:36 AM)


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InvisibleStriplessRED
Registered: 09/05/07
Posts: 562
Loc: Helena
Re: F2 strategy [Re: covertjoy]
    #28477352 - 09/21/23 05:25 AM (4 months, 4 days ago)

Quote:

covertjoy said:

When spores are created by the F1 fruit the genome is recombinated such that each spore has it's own unique haploid genome. When it comes to producing your F2 generation you can germinate spores from a single F1 fruit and yes they will mate with each other, producing a wide range of phenotypes.






Perfect, I wasn't sure which haploid copies an F1 body would produce. Thank you


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