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TeemosStash
Stranger
Registered: 01/28/21
Posts: 1
Last seen: 1 year, 3 months
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Contamination or slow growth?
#28475169 - 09/19/23 03:52 PM (1 year, 3 months ago) |
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Hello everyone!
I have been growing a batch since 8/10 and have noticed that it has had slow growth I was wondering if it is a contamination. There is a slight film over the entire top surface of the substrate that may be contamination but I have also read that it could be a universal veil (This would be the first time I have seen this happen). The colonization period of the substrate was done in non-ideal conditions, mainly with the lighting and temperature (Only getting 2-5 hours of light and temperature fluctuations from 60f to 80f and vice versa). The agar plate had 2 transfers and looked clean. The master jar colonized in about a week then a G2G was performed with those jars colonizing in the same time. The substrate was a mix of coir, vermiculite and gypsum.
Compared to my previous grows I've changed the grain I use from Oats to WBS, added gypsum to the substrate mix, Moved apartments, and finally setup a fruiting tent moving on from a Martha. The average time from spore to getting the fruit was roughly a month plus or minus a week. I used to use my Martha as an all-in-one unit. I would colonize the substrate in the tent then turn on the automation.
I've included 5 images below for anyone that can help me figure out if I am growing some mold or mushrooms. Thank you for your time and help!
I didn't realize that the 2nd image had my lights on but the coloration is like a paper white on the bottom. I also forgot to mention that I am manually controlling the CO2 levels until I can get a fan timer.
   
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meta_mmxxii



Registered: 08/03/23
Posts: 1,071
Loc: PNW
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Re: Contamination or slow growth? [Re: TeemosStash] 1
#28475457 - 09/19/23 06:37 PM (1 year, 3 months ago) |
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I am no expert, but to me it looks like you didn't mix up your spawn and sub very well and all the mycelium is colonized in the bottom of your tub. Like I said I am no expert but that's what it looks like to me.
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Kinoko314
Stranger Danger



Registered: 12/16/22
Posts: 1,585
Loc: Colorado
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Re: Contamination or slow growth? [Re: meta_mmxxii] 2
#28475755 - 09/19/23 10:10 PM (1 year, 3 months ago) |
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That's super bacterial. I don't know if you'll get fruits from that.
Automation hurts more than it helps. I think your setup is over complicated.
Do you have any pics of agar or grain?
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B Traven
Stranger



Registered: 03/10/20
Posts: 3,356
Loc: Central Megalopolis
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Re: Contamination or slow growth? [Re: meta_mmxxii] 1
#28475864 - 09/20/23 04:01 AM (1 year, 3 months ago) |
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Quote:
meta_mmxxii said: I am no expert, but to me it looks like you didn't mix up your spawn and sub very well and all the mycelium is colonized in the bottom of your tub. Like I said I am no expert but that's what it looks like to me.
I'd agree.
Also, like most situations where there's slow growth that someone is concerned about, and based on its appearance in the photos, your spawn is bacterial.
Let this one ride and see how it turns out. You might win the bacterial spawn lotto, you might not.
Also, stop trying to micro-manage the environment. You don't need to be messing with fans or thinking about your CO2 levels. I can't remember what a MARTHA is off the top of my head, so that should tell you how essential I think they are lol
Just go back to the agar work, and try again. For starters, do more than 2 transfers before trying to drop it onto grain.
Gypsum is currently near-universally recognized as completely pointless. A lot of people, myself included, also skip the vermiculite. Straight hydrated coir works just fine.
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