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OfflineZav3r
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Re: Monotub vs Shoebox Question. [Re: Mycolorado]
    #28473360 - 09/18/23 06:14 AM (1 year, 3 months ago)

Yes I know. I didn't transfer them yet. And what do you mean by uniform growth? I can't control how the mycelium grows

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OfflineZav3r
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Re: Monotub vs Shoebox Question. [Re: Mycolorado]
    #28473361 - 09/18/23 06:16 AM (1 year, 3 months ago)

And sorry but honestly it feels like youre just fault finding at this point. I mean this is pretty good growth in agar. It's early in the process so some of them are only 20% colonized. Some of them I put the tissue far from center by mistake. like seriously you don't have to find the little flaws everywhere.

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OfflineSnakeEyes212
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Re: Monotub vs Shoebox Question. [Re: Zav3r]
    #28473369 - 09/18/23 06:30 AM (1 year, 3 months ago)


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Offlinejay.ach
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Re: Monotub vs Shoebox Question. [Re: Zav3r]
    #28473371 - 09/18/23 06:33 AM (1 year, 3 months ago)

I think what he is saying is that you might have better success if you do more transfers before sending to grain.  Examples below. 

Another thing you might consider is running two or three shoe boxes and changing one thing per shoebox - do one and 'set and forget', one where you do misting, and one where you do misting and fanning.  Figure out which one works best.

T0 (also don't use red food coloring in your agar)


T5


T7, this one went to grain


--------------------
“One's destination is never a place, but a new way of seeing things.”  - Henry Miller

LAGM v. 2.023
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OnlineDERRAYLD
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Re: Monotub vs Shoebox Question. [Re: Zav3r]
    #28473372 - 09/18/23 06:34 AM (1 year, 3 months ago)

Quote:

Zav3r said:
And sorry but honestly it feels like youre just fault finding at this point. I mean this is pretty good growth in agar. It's early in the process so some of them are only 20% colonized. Some of them I put the tissue far from center by mistake. like seriously you don't have to find the little flaws everywhere.




:whoak:

You came here asking for advice, you literally asked people to find the little flaws that you're struggling with.

Your agar is very far from good enough whether you like that or not it's still a reality you need to face.
Your agar needs to be cleaned up further.

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OfflineKinoko314
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Re: Monotub vs Shoebox Question. [Re: Zav3r]
    #28473373 - 09/18/23 06:36 AM (1 year, 3 months ago)

If you don't consider your flaws you won't get any better at this.

Non-uniform growth indicates some level of contamination.  If you're not getting uniform growth you should keep transferring until you do.  None of those plates look ready for grain, and some look really rough.  Keep transferring.

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OfflineZav3r
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Re: Monotub vs Shoebox Question. [Re: jay.ach]
    #28473381 - 09/18/23 06:53 AM (1 year, 3 months ago)

Anyone have a guide on this transfer idea?

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OfflineSnakeEyes212
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Re: Monotub vs Shoebox Question. [Re: Zav3r]
    #28473385 - 09/18/23 07:05 AM (1 year, 3 months ago)

Find the fastest developing rhizo growth and cut a triangle piece around it and transfer it? Idk I haven’t started agar yet

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OfflineZav3r
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Re: Monotub vs Shoebox Question. [Re: DERRAYLD]
    #28473420 - 09/18/23 07:59 AM (1 year, 3 months ago)

I'm pretty sure it's not contaminated. Yes it's not even growth but that could be just the way the myc grows. Even after the transfers the myc is growing really well in the grain jars.

That's why I'm saying I've done everything well up to the fruiting conditions. I appreciate the feedback but also some feedback also feels like it's not applicable to the situation.

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OfflineZav3r
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Re: Monotub vs Shoebox Question. [Re: Kinoko314]
    #28473424 - 09/18/23 08:00 AM (1 year, 3 months ago)

You cant be serious at saying that the number one differential of uneven growth is contamination. It can't be anything else? like we're sure that's the problem? Im sorry but I honestly have a really hard time accepting that "uneven growth" without any other visual cues, just the fact that some parts grow faster than others is contamination.

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InvisibleStipe-n CapMDiscord
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Re: Monotub vs Shoebox Question. [Re: Zav3r] * 2
    #28473431 - 09/18/23 08:08 AM (1 year, 3 months ago)

Quote:

Zav3r said:
...what do you mean by uniform growth? I can't control how the mycelium grows




Yes you can. You make transfers until the growth is symetrical, uniform, clean, and healthy. You don't know what you don't know, and we know an awful lot.

This is a germ plate:



The growth is asymmetrical, the milky dots are bacteria. This plate needs to be cleaned, the culture requires further transfers to produce symetrical growth.

These plates lack bacteria, but lack uniform growth:



Here's an example of uniform, symetrical growth. This plate is clean and ready for grain:



You have absolutely no frame of reference. You haven't learned to visually identify problems, you're incapable, utterly and completely. Your plates need work, they're  far from ready.

You should be thankful that we're helping you. Take it personally if you like but I guarantee you won't last long with that attitude.

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Invisiblestarvinghooker
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Re: Monotub vs Shoebox Question. [Re: Zav3r]
    #28473439 - 09/18/23 08:16 AM (1 year, 3 months ago)

SnC kinda beat me to post, but ill submit this anyway :smile:

Uneven growth is not necessarily contaminated, but it may not be very healthy.  Two main points of agar work: 1.clear myc of contaminates 2.inoculate your grain with the best myc possible

Take a small slice from the healthiest looking area of your plate, ryzo growth if possible.  Preferably using an SAB, transfer that piece to a new plate.  From here, just keep repeating until you have some really nice, clean, uniform/radial growth.

The process can take multiple transfers, determined by a few different factors.  Sometimes 2 or 3 transfers...sometimes 5 or 6.  But when you get that beautiful plate of radial ryzo growth it feels fn good, man.

Of the 20 plates you posted, go through and find the best looking spots to Tfer from and get started.  Some of them you probably won't even wanna use, but that's all good.  Just keep slicing and multiplying.

Good luck, man


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Grab life by the balls and yank on 'em til everything you want comes gushing out in thick, creamy ribbons

Noob like me? Start here!
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OfflineZav3r
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Re: Monotub vs Shoebox Question. [Re: Stipe-n Cap]
    #28473445 - 09/18/23 08:26 AM (1 year, 3 months ago)

I have a frame of reference because I've done microbiology work so I know how contaminations look. They're my first agar work in this field however. I looked at youtube videos on cutting core samples from fruiting bodies to agar. That's what I did and that's why I think the mycelium has uneven growth.


Thanks for all the help. All I'm saying is that the mycelium from my plates look contamination free. for sure uneven growth COULD BE a sign of contamination but I don't think that's a certainty especially without any other visual clues.

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Invisiblestarvinghooker
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Re: Monotub vs Shoebox Question. [Re: Zav3r]
    #28473446 - 09/18/23 08:27 AM (1 year, 3 months ago)

There seems to be one thing you arent understanding: healthy myc grows uniformly. There is a difference between contaminated and healthy.

I think you'd do well to take everything you "believe" up to this point and shred that shit. 

Then, instead of arguing with people who've been growing for 30 or 40 years, read what they've posted and ask questions when you need clarification.  You won't find a better resource than here for learning mushcult. I highly recommend not arguing with these people.  If that's what ya wanna do, I hear reddit is always looking for new converts.


--------------------
Grab life by the balls and yank on 'em til everything you want comes gushing out in thick, creamy ribbons

Noob like me? Start here!
Hitchhiker's Guide to the Shroomery


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OfflineZav3r
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Re: Monotub vs Shoebox Question. [Re: starvinghooker]
    #28473448 - 09/18/23 08:28 AM (1 year, 3 months ago)

Thanks but too late. I already transferred all of them to grain jars lol

Grain jars look good though. I don't have pics now though.

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OfflineRoscoeReturnsS
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Re: Monotub vs Shoebox Question. [Re: Zav3r]
    #28473486 - 09/18/23 09:07 AM (1 year, 3 months ago)

I’m sure this will end well.

:popcorn:

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OfflineZav3r
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Re: Monotub vs Shoebox Question. [Re: RoscoeReturns]
    #28473497 - 09/18/23 09:19 AM (1 year, 3 months ago)

I will definitely let you know

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InvisibleMycolorado
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Re: Monotub vs Shoebox Question. [Re: Zav3r] * 2
    #28473507 - 09/18/23 09:23 AM (1 year, 3 months ago)

Quote:

Zav3r said:
I have a frame of reference because I've done microbiology work so I know how contaminations look. They're my first agar work in this field however. I looked at youtube videos on cutting core samples from fruiting bodies to agar. That's what I did and that's why I think the mycelium has uneven growth.


Thanks for all the help. All I'm saying is that the mycelium from my plates look contamination free. for sure uneven growth COULD BE a sign of contamination but I don't think that's a certainty especially without any other visual clues.




You know what’s up; No need to ask for any more input, you got this!

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OfflineSnakeEyes212
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Re: Monotub vs Shoebox Question. [Re: Stipe-n Cap]
    #28473516 - 09/18/23 09:29 AM (1 year, 3 months ago)

I really learned something here! Now I just need a pressure cooker and the other supplies and I’ll be ready for agar. I still have plenty of brf and verm so I think I’ll just run that till I’m out, make some prints, take some biopsies( I have those micro centrifuge tubes), and then I’ll be ready. I feel ready already!

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OfflineZav3r
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Re: Monotub vs Shoebox Question. [Re: SnakeEyes212]
    #28473523 - 09/18/23 09:34 AM (1 year, 3 months ago)

I think my transition to agar was a big game changer. Before agar to grains, I did my own spore prints and then inoculated my own spore syringes into grains. 4/5 of those grains are dead from stalling. I just assumed it was some contamination and had to throw it all out. Since going into agar to grain, I don’t have any more stalls. My grain jars look really good. 2/10 are at 100%. The rest around 50-80% colonization.

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