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OfflineJXAllen
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Registered: 08/06/21
Posts: 615
Last seen: 1 month, 9 days
JXAllen's Sterile Water Vials for Spore Swab Soaking. * 3
    #28470684 - 09/16/23 02:29 AM (1 year, 3 months ago)

I decided to do a quick Tek write up for the centrifuge vials of sterile water that I use to soak my spore swabs

To start, I PC'd two jars; one with the vials and one with water. I also PCd a SS bowl to dump the vials into. I PCd everything for 1 hour and allowed it to cool significantly. It was still hot, but handle-able when I moved the jars and bowl to my FFU. I also grabbed a new syringe, needle and gloves.




Next, I dumped the vials, these are 2ml centrifuge vials, into the bowl, loosened the top on the water jar and got my syringe ready. I then began filling each vial between 1/2 and 3/4 of the way, closed the cap and tossed it back into the jar it started in. I refill the syringe as necessary, keeping the lid loosely on between refills. I think this should give me sterile vials with sterile water with very little opportunity for contamination.




That's all there is to it! I've tested a random sampling and they're sterile.




To use these I have a 3D printed block to hold them. I place the sterile swabs into their own separate vial. If it's a spore swab, I just let it soak for a minute. If I'm grabbing spores from a print, I dab it in the water, remove excess on the inside lip of the vial, dab up an eighth of an inch square and then place the swab into the vial to soak for a minute.



Thanks for taking the time to take a look! I hope this is helpful to someone. Please feel free to leave feedback or questions.


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There are only 10 types of people in the world, those that understand binary and those that do not.
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