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OfflineLiamOd96
Stranger

Registered: 08/31/23
Posts: 4
Last seen: 1 year, 7 days
How long for growth on agar?
    #28464784 - 09/10/23 09:18 PM (1 year, 4 months ago)

I just put some old material to agar (48 hours ago) and am wondering about how long I should give it to see growth?

Spores:
I live in CA so before trying to order spores, I am using some 2-year old dried material I had saved away all separately wrapped in foil in zip-locks with descant packets. I am using
-Gill scrapings from 5 different caps on to 5 different plates.
-A small amount of shake from the bottom of the bag onto its own plate.
-A small amount of ground up material out of 4 different capsules put on to 4 different plates.

Agar:
-I am using a malt extract agar pre-mix. 25g to 500 ml H20 (It expired about a year ago and I have a fresh batch on order to re-try)
-A small amount of Gentamicin Sulfate powder in anticipation of contamination (again, also expired, more on order)
-A small amount of corn syrup for dextrose because it was on hand.

What I am expecting:
-lots of contamination + some mycelium to transfer from
-because the sample set I am using is 2 years old, I am not expecting any kind of growth from the old fruiting body material, but I was hoping that kept in a cool dry and sterile place, there would still be some viable spores in there to start a mycelium culture.

What I have after 48hrs:
-Nothing. Not even contamination growth.
-On the plus side, that means my sterile technique is still good...
-How long should I expect to wait for any sort of growth??

Will repeat experiment again when I get my new agar malt dextrose premix in. I am trying to avoid (what I have read to be) the difficult process of getting spores shipped out here...

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OfflineKinoko314
Stranger Danger
Male


Registered: 12/16/22
Posts: 1,585
Loc: Colorado Flag
Last seen: 4 months, 1 day
Re: How long for growth on agar? [Re: LiamOd96]
    #28464795 - 09/10/23 09:36 PM (1 year, 4 months ago)

Spores can take as few as 3 days to germinate in the best of conditions.  With old spores it will probably take at least 7-10 days, but could take as long as 3 weeks.

I think you can get LC out there.  The company Myyco (on the trusted vendors list) is based in Cali.

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Offlinethcee
Villiage Idiot


Registered: 04/17/23
Posts: 123
Loc: Orbit
Last seen: 1 month, 18 days
Re: How long for growth on agar? [Re: Kinoko314]
    #28464873 - 09/10/23 11:59 PM (1 year, 4 months ago)

Message me if you want some fresh LC. You'll have a much better success rate.


--------------------
:sporedrop:๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ :skittles2:


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OnlineDERRAYLD
Constructus
Male User Gallery


Registered: 05/13/02
Posts: 12,101
Loc: South Africa
Last seen: 45 seconds
Trusted Cultivator
OG Cultivator
Re: How long for growth on agar? [Re: thcee]
    #28464957 - 09/11/23 01:52 AM (1 year, 4 months ago)

Quote:

thcee said:
Message me if you want some fresh LC. You'll have a much better success rate.




Be careful sending anything out to such new members, the market has restrictions for a reason.

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OfflineBustaboo
Calmer than you are


Registered: 07/17/23
Posts: 119
Last seen: 5 months, 5 days
Re: How long for growth on agar? [Re: DERRAYLD]
    #28465097 - 09/11/23 07:34 AM (1 year, 4 months ago)

Iโ€™ve heard that old spores sometimes need to be hydrated a bit to get them going.

Edited by Bustaboo (09/11/23 07:35 AM)

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OfflineKinoko314
Stranger Danger
Male


Registered: 12/16/22
Posts: 1,585
Loc: Colorado Flag
Last seen: 4 months, 1 day
Re: How long for growth on agar? [Re: Bustaboo]
    #28465108 - 09/11/23 07:59 AM (1 year, 4 months ago)

Yeah, that's true.  I usually just put my plate on the heat mat I got with my stupid kit when I started, on a fairly low setting.  This will cause some condensation, which will hydrate the spores.

I did this recently with some nats spores.  First time I waited about 10 days without germination, then I did the thing to hydrate them, and the next morning I had germination.  I did another plate, and this time I hydrated them right away.  It only took 3 days for germination.

I had a similar experience a while back, where the spores didn't germinate for 3 weeks.  Not until I took action did I get germination.

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