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Offlinejbutts
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Registered: 09/10/23
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Help Identifying Contaminant
    #28464774 - 09/10/23 09:08 PM (1 year, 4 months ago)

I recently purchased several spore syringes and am having trouble with one species - Thai Elephant Dung.

My first syringe of this species I spawned to grain and after less then 48 hours the entire quart jars were entirely covered with a wispy, weblike, dull grey contaminant.

The supplier sent me another syringe which I introduced to agar plates and after 36 hours the plates were again entirely covered.

After about a week I can now see where the black spores are; there must be active mycelium around them as there are rings were the webby contaminant is being pushed back.

The inclosed photo is plate growth 48 hours after inoculating with several drops from spore syringe.

Can anybody suggest what this might be? Thanks for the insight!


Edited by jbutts (09/10/23 09:30 PM)

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Offlinetryptkaloids
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Re: Help Identifying Contaminant [Re: jbutts]
    #28464782 - 09/10/23 09:17 PM (1 year, 4 months ago)

Sounds like a mold.  Please upload images directly to the site


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Offlinejbutts
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Re: Help Identifying Contaminant [Re: tryptkaloids]
    #28464790 - 09/10/23 09:30 PM (1 year, 4 months ago)

Sorry about that! I think I fixed my original post.

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OfflineCocaineBuffet
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Re: Help Identifying Contaminant [Re: jbutts] * 1
    #28464793 - 09/10/23 09:34 PM (1 year, 4 months ago)

You are probably putting way too much spore solution on the plate. Do a single drop. When I say a single drop I mean 1 DROP. Get an inoculation loop or sterile swab to streak the plate in a Z pattern

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OfflineKinoko314
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Re: Help Identifying Contaminant [Re: CocaineBuffet]
    #28464800 - 09/10/23 09:42 PM (1 year, 4 months ago)

In 48 hours you wouldn't have germinated spores yet. That has to be straight mold.

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Offlinejbutts
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Re: Help Identifying Contaminant [Re: Kinoko314]
    #28464812 - 09/10/23 09:56 PM (1 year, 4 months ago)

Yes Kinoko, that was my thought process.

The vendor told me the plates looked fine and Thai Elephant Dung was just an aggressive subspecies but based on the fact that whatever was in that syringe overtook an entire quart jar of grain as well as plate of agar in 48 hours makes me reject this.

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Offlinejbutts
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Re: Help Identifying Contaminant [Re: CocaineBuffet]
    #28464815 - 09/10/23 09:57 PM (1 year, 4 months ago)

Quote:

CocaineBuffet said:
You are probably putting way too much spore solution on the plate. Do a single drop. When I say a single drop I mean 1 DROP. Get an inoculation loop or sterile swab to streak the plate in a Z pattern




Thank you for the insight. As a matter of fact this is exactly what I was trying to do. One drop offset centre and streaked with a flame sterilized loop. I underestimated how much comes out of the syringe with a small push. :wink:

Edited by jbutts (09/10/23 09:59 PM)

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