|
jbutts
Stranger
Registered: 09/10/23
Posts: 4
Last seen: 1 year, 4 months
|
Help Identifying Contaminant
#28464774 - 09/10/23 09:08 PM (1 year, 4 months ago) |
|
|
I recently purchased several spore syringes and am having trouble with one species - Thai Elephant Dung.
My first syringe of this species I spawned to grain and after less then 48 hours the entire quart jars were entirely covered with a wispy, weblike, dull grey contaminant.
The supplier sent me another syringe which I introduced to agar plates and after 36 hours the plates were again entirely covered.
After about a week I can now see where the black spores are; there must be active mycelium around them as there are rings were the webby contaminant is being pushed back.
The inclosed photo is plate growth 48 hours after inoculating with several drops from spore syringe.
Can anybody suggest what this might be? Thanks for the insight!
Edited by jbutts (09/10/23 09:30 PM)
|
tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,738
Loc: Exact Center
Last seen: 5 days, 14 hours
|
Re: Help Identifying Contaminant [Re: jbutts]
#28464782 - 09/10/23 09:17 PM (1 year, 4 months ago) |
|
|
Sounds like a mold. Please upload images directly to the site
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
|
jbutts
Stranger
Registered: 09/10/23
Posts: 4
Last seen: 1 year, 4 months
|
|
Sorry about that! I think I fixed my original post.
|
CocaineBuffet
Stranger


Registered: 08/29/19
Posts: 4,271
Last seen: 7 hours, 53 minutes
|
Re: Help Identifying Contaminant [Re: jbutts] 1
#28464793 - 09/10/23 09:34 PM (1 year, 4 months ago) |
|
|
You are probably putting way too much spore solution on the plate. Do a single drop. When I say a single drop I mean 1 DROP. Get an inoculation loop or sterile swab to streak the plate in a Z pattern
|
Kinoko314
Stranger Danger



Registered: 12/16/22
Posts: 1,585
Loc: Colorado
Last seen: 4 months, 1 day
|
|
In 48 hours you wouldn't have germinated spores yet. That has to be straight mold.
|
jbutts
Stranger
Registered: 09/10/23
Posts: 4
Last seen: 1 year, 4 months
|
Re: Help Identifying Contaminant [Re: Kinoko314]
#28464812 - 09/10/23 09:56 PM (1 year, 4 months ago) |
|
|
Yes Kinoko, that was my thought process.
The vendor told me the plates looked fine and Thai Elephant Dung was just an aggressive subspecies but based on the fact that whatever was in that syringe overtook an entire quart jar of grain as well as plate of agar in 48 hours makes me reject this.
|
jbutts
Stranger
Registered: 09/10/23
Posts: 4
Last seen: 1 year, 4 months
|
|
Quote:
CocaineBuffet said: You are probably putting way too much spore solution on the plate. Do a single drop. When I say a single drop I mean 1 DROP. Get an inoculation loop or sterile swab to streak the plate in a Z pattern
Thank you for the insight. As a matter of fact this is exactly what I was trying to do. One drop offset centre and streaked with a flame sterilized loop. I underestimated how much comes out of the syringe with a small push.
Edited by jbutts (09/10/23 09:59 PM)
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 163 topic views. 14 members, 90 guests and 12 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|