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Offlineklars
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Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row.
    #28464460 - 09/10/23 02:57 PM (4 months, 16 days ago)

I started the journey a few months ago. I'm trying to learn the full cycle so I can always maintain at least some form of access and learn to get better genetics over time instead of relying on buying LC or SS.

I've been able to make spawn from a SS of B+, do a g2g transfer successfully, grow out into a monotub and harvest 3 flushes from it so far. Still no contam. I have some clone samples on agar that I'm waiting to see what happens with now. But what I'd realy like is to be able to have some nice spore prints since I know the cycle has to repeat eventually, plus more agar practice.

Problem is that I've not been able to make any sort of spore print at all. I've tried mushrooms from all 3 flushes and I've gotten nothing. I've read tons of posts and watched videos and  tried to find any information on mushroms not producing spores and I'm coming up with nothing.

I've picked them right before the veil breaks (and cutting the veil away), just after breaking, several days after, and over a week and before it starts to look bad. I've never seen spores on the cake or the stem and the gills never look dark.

I've tried bod's guide of tenting with tin foil in a SAB. I've tried to put individual glasses over them to retain moisture. I've tried putting a drop of water on the cap before covering them for what ever reason the person suggested. I've left them up to 48 hours without being disturbed and I get nothing but condensation. Even after it tries it looks like nothing is there. I've searched for pictures of B+ spore prints and they are all dark so I'm sort of expecting something more than invisible spores.

I've come across conflicting information about how caps won't drop spores if they aren't humid enough. I've also seen a guy put damp rid in the container to purposely absorb moisture so that he does get a good spore print....

At this point I'm about to try and use sterile cotton swabs to see if i can get something by swabbing the gills directly once this next flush opens up in a few days.

RH inside the box is usually around 90-95%. They never feel dry and there is always condensation on the side of the box. The brick has shrunk quite a bit but not I'm not that plays into any of it.

Thanks for the help and suggestions.


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OfflineScrewup
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars]
    #28464462 - 09/10/23 03:01 PM (4 months, 16 days ago)

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InvisiblebodhisattaMDiscordReddit
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars]
    #28464463 - 09/10/23 03:02 PM (4 months, 16 days ago)

Pics of the fruits?


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OfflineThe Tao
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars]
    #28464484 - 09/10/23 03:30 PM (4 months, 16 days ago)



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Offlineklars
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: bodhisatta]
    #28464485 - 09/10/23 03:31 PM (4 months, 16 days ago)



I think I've uploaded them correctly.

I don't have pictures of the previous attempts but these are the three that I let grow for a while in my last attempt wondering if they just needed more time. I also took a picture of a cluster that hasn't had the veil broken yet for the next attempt.

They darkened over time after being plucked. The gills were very much an offwhite color when first picked.


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OfflineVP123
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars]
    #28464531 - 09/10/23 04:31 PM (4 months, 15 days ago)

I'm trying to figure out what is going on. Are you telling us that if you let those mushrooms mature without picking them, they will reach the point where they begin to rot without dropping spores?


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Offlineklars
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: VP123]
    #28464539 - 09/10/23 04:39 PM (4 months, 15 days ago)

I am still learning and have not left them till they begin to rot to my knowledge. Most everything I read talks about picking them right after the veil breaks to make prints or waiting till you see some spores drop on the stem. I’ve left them growing till the caps start to crack and the edges start to get ragged and folding. About 10 days after opening. Still no spores so I picked them so they wouldn’t rot but I could be misjudging. I’m happy to leave these new ones growing all the way till they are rotting just to experiment. This is the 4th flush and I’ve gotten a nice harvest out of this cake already.

I’ll start taking daily pictures of them for reference and try any suggestions. I could be missing something very basic.


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OfflineVP123
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars] * 1
    #28464543 - 09/10/23 04:50 PM (4 months, 15 days ago)

I would let them grow and let the caps fully open and more (when you see spores dropping). The recommendation to harvest as soon as the veil breaks is in order to avoid them dropping spores (presumably the taste is affected if they drop a lot of spores).

If you want to take spores prints you have to let them mature to the point they begin to drop spores. You got this. Don't despair. B+ are known for dropping lots of spores. You can get thick spore prints from them.


Edited by VP123 (09/10/23 04:53 PM)


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Offlineklars
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: VP123]
    #28464555 - 09/10/23 05:05 PM (4 months, 15 days ago)

Thanks. Spores or rot. Here we go. If nothing else I’ll learn something. Going to keep track of the days since open till I see something drop on the stem or cake and probably take photos along the way.


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OfflinePBJ710
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars] * 1
    #28464590 - 09/10/23 05:38 PM (4 months, 15 days ago)

Try waiting until you see a slight purple color showing on the top of the stipe/veil remnant then harvest & print the cap. 

I like to wait until mine are opened up and flat on top before harvesting for prints.  Harvesting right before/as the veil breaks is for bag appeal since there won't be spores everywhere to dirty up things.


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Offlineklars
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: PBJ710]
    #28464852 - 09/10/23 11:09 PM (4 months, 15 days ago)

That makes sense. I assume that ring is where the spores dropping are collecting on the remnant on the veil on the stem. That's a good idea. Thanks. Party might be over though. I just found what I think is the beginning of lipstick mold?

I know I need to toss the cake but are the fruits that just broke veil still ok to keep?

Bound to happen eventually. I have another half tub half colonized with the g2g spawn I made from the original grain. I'll get another shot at making spore prints at least.




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OfflinePBJ710
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars]
    #28464892 - 09/11/23 12:22 AM (4 months, 15 days ago)

Looks like fusarium IMO - you can put some salt on the pink stuff and cover it with a piece of paper towel to help contain any spores from it, but theres no guarantees that you will be able to contain them.  It would be wise to assume that the prints from that tub will be contaminated to some degree and use agar to clean up any cultures before expanding them to grains or PFtek though.


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Offlinecozmyc
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars]
    #28464915 - 09/11/23 01:02 AM (4 months, 15 days ago)

Quote:

klars said:


I think I've uploaded them correctly.

I don't have pictures of the previous attempts but these are the three that I let grow for a while in my last attempt wondering if they just needed more time. I also took a picture of a cluster that hasn't had the veil broken yet for the next attempt.

They darkened over time after being plucked. The gills were very much an offwhite color when first picked.



Do you have the caps flipped for the pictures? If not, prints should be taken with the gills down. You should be able to see the spores after a little while and can take multiple prints from a single cap.:mushroom2:


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OfflineDERRAYLD
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: cozmyc]
    #28464953 - 09/11/23 01:50 AM (4 months, 15 days ago)

I was thinking the same thing, did you not just put the caps the wrong way round?
The one looks very dark which should have dropped a print.


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Offlineklars
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: PBJ710]
    #28465102 - 09/11/23 07:44 AM (4 months, 15 days ago)

I appreciate it. This is the 4th flush so I’m just sort of experimenting now. I did just as you said after reading what you suggested. I’m going to let these keep growing to see if they eventually start dropping spores. I took and put some white index cards under some of the other ones that have open veils too to see if I eventually see spores dropping that way.


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Offlineklars
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: DERRAYLD]
    #28465103 - 09/11/23 07:46 AM (4 months, 15 days ago)

I turned them over for the pictures. I figured seeing the gills gave more information than seeing the tops of the caps sitting on foil. I’ve had them sitting on foil for 72 hours now and there is just condensation under the caps and nothing that looks like spores. Even the dark one. Just clean foil and a few water droplets.


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OfflineDERRAYLD
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars]
    #28465104 - 09/11/23 07:50 AM (4 months, 15 days ago)

Odd,  have you tried swabbing the gills?


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Offlineklars
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: DERRAYLD]
    #28465283 - 09/11/23 11:21 AM (4 months, 15 days ago)

Not yet. I'm about to order some sterilized ones and give that a shot. If nothing else just to see if anything comes off. If they are completely clean after that I'll be very confused.


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OfflineBajazly
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: klars]
    #28465428 - 09/11/23 01:36 PM (4 months, 15 days ago)

SirPsyco not long ago had a strain that produced no spores or maybe it was no gills, cant quite remember but maybe this is a similar occurrence.


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Offlinefatpup
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Re: Attempting to make spore prints of B+. Failing to produce spores 3 flushes in a row. [Re: PBJ710]
    #28465680 - 09/11/23 06:18 PM (4 months, 14 days ago)

Not to flame. But the pics you have are gill side up. You are putting the gills side down on the foil right?

All the b+ i have grown throws sprores everywhere if you let it mature.


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