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OfflineHugh Jass
Stranger

Registered: 02/17/23
Posts: 1,052
Last seen: 13 hours, 1 minute
Moldy agar...
    #28463961 - 09/10/23 02:42 AM (1 year, 4 months ago)

I'm growing mold from my spores.


Using a SAB I made up lots of petri dishes. I was just interested in what I could grow on them.
I put a couple of grains of already colonized seed on some and all I have grown is mycillium.
I wondered what kind of bacteria I had on my hands and made thumb and finger prints on the agar and nothing is growing there.

I also left a couple blank, to try and work out if I had really bad technique. They are growing nothing, which I am surprised at.

I scraped spores onto the agar and I am growing lots of different colour mold from the spores.


I have read that tinfoil is sterile so I did not wipe the tin foil with alcohol or anything, I just threw the first 6" away and used the rest of the roll.
I put the schroom caps on it and then put the foil in a plastic tub that I had wiped down with alcohol and put pint glasses over the top and put the lid on the tub.

I grow in a 4X4 tent, because it keeps the temp at about 27'c, I vent to outside, but I do not filter the incoming air, could this be the problem?
Is the incoming air to the tent full of bad spores?

Looking at the agar the mold is coming directly from the black spores.

What can I do better?

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OfflineHysteria
Bum Looker


Registered: 02/19/23
Posts: 996
Loc: New England USA
Last seen: 1 month, 26 days
Re: Moldy agar... [Re: Hugh Jass]
    #28463963 - 09/10/23 02:47 AM (1 year, 4 months ago)

Are you taking 2 prints from each cap as in Bod's spore print tek? If not, check out his tek. The 2nd print should come out much cleaner.

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OfflineHugh Jass
Stranger

Registered: 02/17/23
Posts: 1,052
Last seen: 13 hours, 1 minute
Re: Moldy agar... [Re: Hysteria]
    #28463971 - 09/10/23 03:38 AM (1 year, 4 months ago)

Quote:

Hysteria said:
The 2nd print should come out much cleaner.




Ah, I only did one print.

Will try the 2nd print next time.

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OfflineKinoko314
Stranger Danger
Male


Registered: 12/16/22
Posts: 1,585
Loc: Colorado Flag
Last seen: 4 months, 1 day
Re: Moldy agar... [Re: Hugh Jass]
    #28464121 - 09/10/23 09:10 AM (1 year, 4 months ago)

Do you have this air blowing around while you are working in your SAB?  If so you probably aren't getting still air.

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Invisiblethirdeyewild
Male User Gallery


Registered: 11/01/13
Posts: 1,376
Loc: Flag
Trusted Cultivator
Re: Moldy agar... [Re: Kinoko314]
    #28464131 - 09/10/23 09:33 AM (1 year, 4 months ago)

Streaking is superior to scraping.
Streaking thins out the concentration of spores and contaminants allowing a higher chance for the spores to exploit some clean agar.
Scraping leaves dense clusters of spore and contaminant.

To streak, use a scalpel or inoculation loop and take the smallest amount of spores possible from your print.
From there I usually draw a tic tac toe board (if using scalpel, if loop I zig zag back and forth while moving across the plate) on the agar but you could do more lines if you wish.


--------------------

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OfflineHugh Jass
Stranger

Registered: 02/17/23
Posts: 1,052
Last seen: 13 hours, 1 minute
Re: Moldy agar... [Re: Kinoko314]
    #28464600 - 09/10/23 05:44 PM (1 year, 4 months ago)

Quote:

Kinoko314 said:
Do you have this air blowing around while you are working in your SAB?  If so you probably aren't getting still air.




No the SAB is in a different room , heater off, windows shut, dust mask on

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