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OfflineAmygdala
Learner

Registered: 06/30/04
Posts: 158
Last seen: 8 years, 6 months
Flame sterilization of syringe
    #2846180 - 07/01/04 03:17 AM (19 years, 7 months ago)

Hello all, I plan on innoculating upon receipt of an answer, but i'd like to be clear on this. The TEK i will be using suggests flame sterilization of the syringe via a lighter, in between each cake inoculation. Is this really needed? My main concern is heating the tip so frequently may put the spores at a high risk. Does anyone adhere to this rule on some level? Maybe even a single sterilization at the beginning? :stoned:


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Invisibleshriek
*********

Registered: 12/13/03
Posts: 3,274
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846185 - 07/01/04 03:22 AM (19 years, 7 months ago)

i never flame starilize syringes, i have never had a problem with contam because of that. but i think i live in a area with low contams, i hardly bother to sterilize spawn media and casigns.


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InvisibleStarter
Stranger
 User Gallery

Registered: 05/16/03
Posts: 1,148
Loc: Australia
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846192 - 07/01/04 03:32 AM (19 years, 7 months ago)

If it's your first time out, use the flame. Later on when you're making your own spore syringes ect then be more cavalier.


--------------------
Convert Metric and Imperial.


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OfflineHrethic
A Human, Being

Registered: 01/05/04
Posts: 2,397
Loc: Passing through the bulk Flag
Last seen: 2 years, 10 months
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846193 - 07/01/04 03:33 AM (19 years, 7 months ago)

Everything depends on a million different factors all going in your favor. All you can do is give the few that you can alter a little boost in your favor. :mushroom2:


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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 10 minutes
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846198 - 07/01/04 03:36 AM (19 years, 7 months ago)

>Maybe even a single sterilization at the beginning?

Providing the jars are sterile, and you don't touch anything else but the jars with the syringe, then this is sufficient.


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Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846276 - 07/01/04 05:17 AM (19 years, 7 months ago)



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Offlinehawksapprentice
Yearns to Snowboard
 User Gallery

Registered: 06/06/03
Posts: 3,195
Loc: Oregon
Last seen: 8 months, 24 days
Re: Flame sterilization of syringe [Re: matts]
    #2846335 - 07/01/04 06:08 AM (19 years, 7 months ago)

ROFL @ matt. Thats pretty funny dude.


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"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


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