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OfflinegoofyYno
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Registered: 09/08/23
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Loc: Arizona, United States
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noob question oyster lc to lc vs spawn
    #28462394 - 09/08/23 12:58 PM (4 months, 18 days ago)

Hi all, first timer here.
I have read a bunch and am very excited to jump into the deep end.  But I got a little confused and cannot see to find the right answers with my searches.  Looking for a little help and kindness.
  Here is where I'm at (have not started anything).  I ordered 2 10 ml syringes of pink and phoenix oysters.  I had planned on starting bag so I bought 3T unicorn bag  and straw pellets. When I contacted seller about how much to put in each bag he said I'm wasting money to go straight  to substrate.  I remembered for spores you need to let the grow first, so I bought jars (16 oz mason) and Karros syrup, distilled water and marbles to make LC.  But then I looked and the syringes are LC not spores, so am I supposed to make spawn with them?  I guess I could get some grain and use the jars for this before I put it in substrate?

so  1) should I use my bought lc to expand into more lc or put in grain for spawn?
2) are the 16 oz jars too small for this? should I get bigger quart jars?
3) how much of the 10 cc should I use?
thanks for the help


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OfflinetusJus
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Registered: 11/19/22
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Re: noob question oyster lc to lc vs spawn [Re: goofyYno]
    #28462514 - 09/08/23 03:44 PM (4 months, 17 days ago)

Many people who tried the lc to bulk route had bad results (me too). With bulk I mean the the bulk substrate like sawdust or in your case the straw. Although lc -> bulk worked for some I recommend you start with the “normal” way of lc -> grain -> bulk.

At the bottom of the gourmet forum you should see a box labeled “Search forum”. If you search for “lc to bulk” you find some posts where people asked and answered that same question.


I prefer bigger jars but you can use the ones you have. It might even be easier to handle the small jars in the SAB. Do you have a SAB (still air box)? You don’t really need one for this, if you are using injection ports on your jars.

I wish you the best. When you follow a proven tek, failure is less likely so do that especially in the beginning.

(Also don’t put spores into lc, put them on agar. One usually doesn’t grow gourmets from spore but you should get familiar with agar eventually.)

I can’t really answer your 3 questions.


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OfflineSHROOMSISAY01M
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Re: noob question oyster lc to lc vs spawn [Re: tusJus]
    #28462854 - 09/08/23 10:10 PM (4 months, 17 days ago)

I would put the LC to some whole oats I use a mixture of whole oats and millet but whole oats will work well enough. I always make at least two bags just in case one does not work out. I always make sure each bag of spawn is big enough to make the bags I plan on making that week plus enough to make 2 more bags of spawn by grain-to-grain transfer. I would not do LC after you get started. Instead do G2G transfers. The problem with LC is you do not know if it is any good until it goes to grain. So you are potentially wasting time, effort, and money doing LC. I would suggest using 14A bags for your spawn and grow bags and not using mason jars. Take your time and be clean you you will be successful. I would also suggest you buy some of the reclosable cable ties to close the bags after spawning they are cheap and work very well for this purpose. By the way, only fold the bags when pressure cooking. After pressure cooking the bags will make a tight seal against themselves as they cool to keep out any contamination...

Cable ties

https://www.amazon.com/Monoprice-Releasable-cable-50LBS-100pcs/dp/B004C4ZRIK/ref=sr_1_5?crid=2V3V64OKBC46Y&keywords=monoprice%2Bcable%2Bties&qid=1694232257&sprefix=monoprice%2Bcable%2Bties%2Caps%2C103&sr=8-5&th=1


14A Filter Patch Bags

https://myersmushrooms.com/product/14a-mushroom-grow-bags-5-7-lb-5-micron/


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OfflinegoofyYno
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Registered: 09/08/23
Posts: 7
Loc: Arizona, United States
Last seen: 2 months, 16 days
Re: noob question oyster lc to lc vs spawn [Re: SHROOMSISAY01]
    #28462884 - 09/08/23 11:15 PM (4 months, 17 days ago)

Now I'm really confused.  That was my original plan.  I have these bag :

Unicorn Bags 3T - 8″ x 5″ x 19 - 2.2 Mil - 1.5” x 1.5” 0.2 micron Filter - Typical Block Size: 5-7 lbs

So are you saying it would be best to use the 10 cc on how many bags?
then go G2G to propagate, and what would be best for fruiting?


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OfflineSHROOMSISAY01M
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Re: noob question oyster lc to lc vs spawn [Re: goofyYno]
    #28463227 - 09/09/23 10:28 AM (4 months, 17 days ago)

Quote:

goofyYno said:
Now I'm really confused.  That was my original plan.  I have these bag :

Unicorn Bags 3T - 8″ x 5″ x 19 - 2.2 Mil - 1.5” x 1.5” 0.2 micron Filter - Typical Block Size: 5-7 lbs

So are you saying it would be best to use the 10 cc on how many bags?
then go G2G to propagate, and what would be best for fruiting?




I have never actually used LC it is just a bad choice in my opinion. I buy grain spawn from a reputable supplier like Amycel, North Spore, or Aloha Medicinal. With one bag you can have great genetics for many years so it is worth it in my opinion. I don't think you can buy spawn from Aloha at the moment. I then make a master slant from a single grain. So I will have culture for many years and I can just pull from the master if need be. No need to worry about any of that right now you have LC use it. I would ask how many cc to use of LC for a 5 lb bag. Once you have a good bag of grain just do G2G (grain to grain) transfers and you can keep going for years. If your gain is bad you will have many queues to let you know, unlike LC.

For fruiting, I would use the 14A bags. I use 14A bags for my spawn also and I have not had a problem to this point. After putting the spawn in the sub bag use a cable tie to seal the bag above the filter patch leaving as much air in the bag as possible. Once the sub bag has fully colonized you release the cable tie and move the tie all the way down to the sub to remove as much air as possible. Then you take a razor blade or similar and slice the bag you don't want to lift the bag from the sub when slicing you don't want air to get in the bag if it does it is fine but you will get pins under the bag that won't develop properly. This is why I slice the bottom of the bag where it is flat and I can make a clean slice without lifting the bag. That thin slice will form the mushroom pins that will grow a large cluster of mushrooms. Do you have a tent to fruit in or will you be fruiting on a table and misting?


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OfflinegoofyYno
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Registered: 09/08/23
Posts: 7
Loc: Arizona, United States
Last seen: 2 months, 16 days
Re: noob question oyster lc to lc vs spawn [Re: SHROOMSISAY01]
    #28463340 - 09/09/23 12:27 PM (4 months, 17 days ago)

Thank for the help.  I bought from everythingmushrooms on a tip from a different forum. so I thought they were quality.  But if not, I'm fine. just learning and trying to have fun.  If I like it, I will upgrade to a better system/ agar, ect.  Maybe I'll check a drop or two out under the microscope, see what I can find (If my 20 year old scope still works)

Here is what I'd like to do, tell me if I'm making a bigger mistake.

I'd like to take the lc, put 2 ml into a quart jars of oat grain, then use that in a substrate bag for fruiting.  Right now I'm just using a small Instapot for cleaning.    (again if I think I have a future in this, I'll get a bigger pot.)

as for fruiting. I hadn't thought that far ahead. I was going to use part of a spare bedroom and build martha tent. but I could use our harry's closet under the stars.  The benefit would be enclosed room to get humidity up (not much in arizona) but worried about air flow.

Question about your tie. Is the idea to remove the extra room in the bag over the vent? then do you slice near the bottom and tie below the vent so the only air is though the bottom slit?


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OfflinegoofyYno
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Registered: 09/08/23
Posts: 7
Loc: Arizona, United States
Last seen: 2 months, 16 days
Re: noob question oyster lc to lc vs spawn [Re: goofyYno]
    #28463555 - 09/09/23 05:26 PM (4 months, 16 days ago)

maybe I'm being too impulsive, I didn't want to make the agar, but i did just by 10 plates.  so at least I can plate some. and, good news my microscope does indeed work!


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OfflinegoofyYno
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Registered: 09/08/23
Posts: 7
Loc: Arizona, United States
Last seen: 2 months, 16 days
Re: noob question oyster lc to lc vs spawn [Re: goofyYno]
    #28463600 - 09/09/23 06:19 PM (4 months, 16 days ago)

well, I'm having fun with my own thread, but as my impulsivity erupts (did I say I was adhd?) , I have been reading and my initial thoughts of fea problems in a closet seem to ring true, so I think I'm headed towards a martha's tend.  ( I am adding in case some wise mentor reads and wants to advise).


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Offlinedeadmandave
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Registered: 02/16/10
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Re: noob question oyster lc to lc vs spawn [Re: goofyYno]
    #28463688 - 09/09/23 07:41 PM (4 months, 16 days ago)

Put an exhaust fan in the closet that pulls air outside and it'll work fine.

You can use 1ml per quart jar, probably even less.  (I also don't fuck around with lc so take my advice with a grain of salt)

Keep an eye out at thrift stores for the bigger prestos or if you like this hobby invest in a used AA autoclave. One of the best purchases you can make.


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OfflineSHROOMSISAY01M
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Re: noob question oyster lc to lc vs spawn [Re: goofyYno]
    #28465810 - 09/11/23 08:27 PM (4 months, 14 days ago)

Quote:

goofyYno said:
Thank for the help.  I bought from everythingmushrooms on a tip from a different forum. so I thought they were quality.  But if not, I'm fine. just learning and trying to have fun.  If I like it, I will upgrade to a better system/ agar, ect.  Maybe I'll check a drop or two out under the microscope, see what I can find (If my 20 year old scope still works)

Here is what I'd like to do, tell me if I'm making a bigger mistake.

I'd like to take the lc, put 2 ml into a quart jars of oat grain, then use that in a substrate bag for fruiting.  Right now I'm just using a small Instapot for cleaning.    (again if I think I have a future in this, I'll get a bigger pot.)

as for fruiting. I hadn't thought that far ahead. I was going to use part of a spare bedroom and build martha tent. but I could use our harry's closet under the stars.  The benefit would be enclosed room to get humidity up (not much in arizona) but worried about air flow.

Question about your tie. Is the idea to remove the extra room in the bag over the vent? then do you slice near the bottom and tie below the vent so the only air is though the bottom slit?




I know nothing about everythingmushrooms genetics and genetics means everything. Since you already have the LC you have nothing to lose at this point just use them.

As far as a fruiting room I would just buy one from Amazon or Walmart it will end up being cheaper than trying to build one. I would get one that is clear not green...

https://www.amazon.com/s?k=green+house&crid=2MWYQWKHEO0E3&sprefix=green+house%2Caps%2C134&ref=nb_sb_noss_2

If you have the space I would buy this one...

https://www.amazon.com/s?k=green+house&crid=2MWYQWKHEO0E3&sprefix=green+house%2Caps%2C134&ref=nb_sb_noss_2

If you buy a smaller one you will have a hard time putting a circulation fan inside. If you don't keep the air moving you will get long stipes (stems).

You tie the bag above the filter patch so there is enough air exchange. When you tie it, leave as much air as you can in the bag. Once the sub is fully colonized you move the tie down to the sub. So there is no extra air. You want it tight against the sub. If you let the sub colonize on a flat surface the sub will be nice and flat on the bottom so then you can slice the bottom of the bag across the seam from corner to corner and because there are no gullies or gaps as there will be on the side you can slice and not have to worry about air getting into the bag and pins starting inside the bag that will not fruit properly and end up being a waste of energy.


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OfflinegoofyYno
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Registered: 09/08/23
Posts: 7
Loc: Arizona, United States
Last seen: 2 months, 16 days
Re: noob question oyster lc to lc vs spawn [Re: SHROOMSISAY01]
    #28466611 - 09/12/23 02:56 PM (4 months, 14 days ago)

Thank for the advice, I think I will buy prebuilt green house. easier and not too expensive.

In other news, I may have cooked my LC anyway.  I did not realize it would be delivered to the mailbox (as opposed to front door) so it sat in the >100 degree heat in an enclosed mailbox for 3 days.  I got some agar plates, and will see if any are still alive.

if not, where and how is the best way to start if lc is a poor choice?


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OfflinegoofyYno
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Registered: 09/08/23
Posts: 7
Loc: Arizona, United States
Last seen: 2 months, 16 days
Re: noob question oyster lc to lc vs spawn [Re: goofyYno]
    #28466640 - 09/12/23 03:25 PM (4 months, 14 days ago)

I just checked,  everything mushrooms is a board vender.  fwiw

I would love to get a big greenhouse, but I doubt 10x12 would fit in the bed room. I wish it was not so hot here and I could set it up in the back yard.


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OfflineSHROOMSISAY01M
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Re: noob question oyster lc to lc vs spawn [Re: goofyYno]
    #28467655 - 09/13/23 02:04 PM (4 months, 13 days ago)

The greenhouse is not 10 x 12 it is 5 x 5 x 6 (LWH). Like I said I would buy grain spawn from either Amycel Medicinal or North Spore. North Spore is a lot easier to order from. Just sterilize your spawn and do a G2G transfer and In 10 days you should be ready to fruit.

here is the greenhouse...

https://www.amazon.com/Outsunny-Portable-Garden-Steeple-Greenhouse/dp/B00HDZEG3W/ref=sr_1_16?crid=2MWYQWKHEO0E3&keywords=greenhouse&linkCode=ll1&qid=1694635339&sprefix=green+house%2Caps%2C134&sr=8-16


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OfflineSHROOMSISAY01M
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Re: noob question oyster lc to lc vs spawn [Re: SHROOMSISAY01]
    #28468243 - 09/13/23 09:48 PM (4 months, 12 days ago)



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