Home | Community | Message Board


RVF Garden Supply
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars

Jump to first unread post. Pages: 1
OfflineAmygdala
Learner

Registered: 06/30/04
Posts: 158
Last seen: 1 year, 3 months
Flame sterilization of syringe
    #2846180 - 07/01/04 05:17 AM (12 years, 5 months ago)

Hello all, I plan on innoculating upon receipt of an answer, but i'd like to be clear on this. The TEK i will be using suggests flame sterilization of the syringe via a lighter, in between each cake inoculation. Is this really needed? My main concern is heating the tip so frequently may put the spores at a high risk. Does anyone adhere to this rule on some level? Maybe even a single sterilization at the beginning? :stoned:


Post Extras: Print Post  Remind Me! Notify Moderator
Invisibleshriek
*********

Registered: 12/13/03
Posts: 3,274
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846185 - 07/01/04 05:22 AM (12 years, 5 months ago)

i never flame starilize syringes, i have never had a problem with contam because of that. but i think i live in a area with low contams, i hardly bother to sterilize spawn media and casigns.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleStarter
Stranger
 User Gallery

Registered: 05/16/03
Posts: 1,148
Loc: Australia
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846192 - 07/01/04 05:32 AM (12 years, 5 months ago)

If it's your first time out, use the flame. Later on when you're making your own spore syringes ect then be more cavalier.


--------------------
Convert Metric and Imperial.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHrethic
A Human, Being

Registered: 01/06/04
Posts: 2,397
Loc: Passing through the bulk Flag
Last seen: 5 months, 2 days
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846193 - 07/01/04 05:33 AM (12 years, 5 months ago)

Everything depends on a million different factors all going in your favor. All you can do is give the few that you can alter a little boost in your favor. :mushroom2:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineAnnoA
Experimenter
 User Gallery

Folding@home Statistics
Registered: 06/18/99
Posts: 24,121
Loc: my room
Last seen: 2 months, 11 days
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846198 - 07/01/04 05:36 AM (12 years, 5 months ago)

>Maybe even a single sterilization at the beginning?

Providing the jars are sterile, and you don't touch anything else but the jars with the syringe, then this is sufficient.


Post Extras: Print Post  Remind Me! Notify Moderator
Invisiblematts
matts

Registered: 01/28/02
Posts: 3,649
Re: Flame sterilization of syringe [Re: Amygdala]
    #2846276 - 07/01/04 07:17 AM (12 years, 5 months ago)



Post Extras: Print Post  Remind Me! Notify Moderator
Offlinehawksapprentice
Yearns to Snowboard
 User Gallery

Registered: 06/06/03
Posts: 3,193
Loc: Oregon
Last seen: 2 months, 27 days
Re: Flame sterilization of syringe [Re: matts]
    #2846335 - 07/01/04 08:08 AM (12 years, 5 months ago)

ROFL @ matt. Thats pretty funny dude.


--------------------
"I celebrate the Earth, my home, my mother, my grave, and as long as men are Man they must, if they would preserve the integrated being, do the same---[and preserve]--this rank casual hungry smelly sweaty lusting transitory body, my oozy pulpy liquid-bag-swollen body, bones, blood, hair glands, my bejeweled sex; I love and celebrate it all.  never to let men forget that they are animals as much as gods---that is one thing I shall say."

  Edward Abbey


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* flame sterilization question Harry Mannbakk 592 6 01/15/09 01:45 PM
by schutzemdown
* Question about flame sterilization jonneill 865 7 06/12/04 01:52 PM
by sublimesubmind
* Time it Takes Needle to Cool after Flame Sterilization?
( 1 2 all )
FallenAngel 3,552 21 11/07/11 01:33 AM
by TheHistoryChannel
* flame sterilization sub_simple 1,577 17 12/03/04 12:50 PM
by spliffmasta
* Flame Sterilizing MrKrinkle 759 13 08/27/05 08:57 PM
by OldSpice
* Question about flame sterilization Xobil3 475 3 03/24/08 03:19 PM
by thimself
* Agar Work & Sterile Tek Questions - PC & Flame Sterilize + Antibotic Agar eltrkbrd 911 0 03/20/07 10:49 AM
by eltrkbrd
* Flame sterilization or alcohol? Velocity92C 1,857 17 08/01/06 02:12 PM
by Frappy

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart
667 topic views. 29 members, 87 guests and 11 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Everything Mushrooms
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.003 seconds on 14 queries.