Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Amanita Muscaria Store Amanita Extract   Kraken Kratom Kratom Capsules for Sale   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Autoflowering Cannabis Seeds   Bridgetown Botanicals CBD Capsules   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   North Spore Boomr Bag

Jump to first unread post Pages: 1 | 2 | 3  [ show all ]
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms * 1
    #28442574 - 08/22/23 09:11 AM (5 months, 3 days ago)

For those who experience anxiety on shrooms...If you take shrooms along with 70mg of pure thh or tetrahydroharmine, not only does it eliminate anxiety, add crystal clear clarity, increased euphoria, but the shroom trip is extended by hours, done it countless times myself, absolutely incredible gorgeous experience. Thh is a psychedelic sri not maoi found in cappi. Damn near perfect psychedelic combo....other than that mescaline is all ready the perfect psychedelic, drank cactus tea over 150 times over many years. I love that it lasts incredibly long allready.

Go to page 3 for an easy pan cyan estero strain grow followed similar to Asura: https://www.shroomery.org/forums/showflat.php/Number/28442574/fpart/3

Asura's method: https://www.shroomery.org/forums/showflat.php/Number/25987049/vc/1

Moonyham said:
Quote:

Sorry I didn't have time to read everything but why the particular mushroom strain? Would this not also work with cubes, subs or other more available mushrooms?



Hi moonyham, this is with the pan cyan grass/dung lovers strain, long considered the crown jewel of mushrooms, slightly harder than cubensis to grow, and the trips are way more visual, very euphoric, very intricate fractal and geometric visuals like acid with saturated tropical colors. Several of my indoor containers contaminated as I did not use mycobags to grow out the substrate...I followed a tek where they were not used, now I know better next time to use them.

More on this variety with 50 trip reports: https://www.shroomery.org/forums/showflat.php/Number/28108398/fpart/1/vc/1

Modern research has determined that the pan cyan strain of mushrooms with their HIGH BAEOCYSTIN and related analogues content hit the adrenal receptors with as much force as MESCALINE or DMT, see chart below. Notice how just plain psilocin alone hits the 3 adrenal receptors (responsible for enhanced colors, euphoria, beauty and music enhancement) with lighter activity.



Attached: Structure–Activity Relationships for Psilocybin, Baeocystin, Aeruginascin, and Related Analogues to Produce Pharmacological Effects in Mice, ACS Pharmacol. Transl. Sci. 2022, 5, 11, 1181–1196:

https://pubs.acs.org/doi/10.1021/acsptsci.2c00177

by Grant C. Glatfelter*, Eline Pottie, John S. Partilla, Alexander M. Sherwood, Kristi Kaylo, Duyen N. K. Pham, Marilyn Naeem, Grant C. Glatfelter*, Eline Pottie, John S. Partilla, Alexander M. Sherwood, Kristi Kaylo, Duyen N. K. Pham, Marilyn Naeem, Vamshikrishna Reddy Sammeta Vamshikrishna Reddy Sammeta, Stacie DeBoer, James A. Golen, Elliott B. Hulley, Christophe P. Stove, Andrew R. Chadeayne, David R. Manke, and Michael H. Baumann.

--------------------------------------------------

Here is the Gordotek pan cyan video followed:

Other topics: Alchemy chemistry fun:

Compilation of pan cyan or panaeolus cyanescens or copeandia cyanescens trip reports, crown jewel of mushrooms:
https://www.shroomery.org/forums/showflat.php/Number/28108398/page/1

You will absolutely love the 100mg THH + pan cyan combo! just like an organic LSD with added mescaline qualities from the THH, in a league of her own, no mental confusion but crystal clear clarity, so diamondlike shimmering beautiful, the euphoria, tropical colored visuals, music-enhancement and healing is divine.

How to extract 2.4g dmt from 170g bark using a 2 Liter erlenmeyer flask (heat and break resistant), post #15:
https://mycotopia.net/topic/111610-hpbcd-dmt-sublingually-active-under-tongue/

Tetrahydroharmine or THH and how to make her, Caapi visionary feminine teaching spirit:
https://www.shroomery.org/forums/showflat.php/Number/28423951/page/1

Zero nausea HPBCD or aloe vera enhanced penetration Ayahuasca capsules:
https://www.shroomery.org/forums/showflat.php/Number/28189371/page/1

Cactus tea before waterpark to beat the heat:
https://www.shroomery.org/forums/showflat.php/Number/28411312/page/4



How to make LSI or Lysergic Acid Isovaleraldemide (Greek Eleusis ancient LSD) at home from morning glory seeds (the priests used non poisonous claviceps paspali which grows on paspalum grass adjacent to Eleusis present day in the famous Rarian plane, same alkaloid profile as the sacred Mesoamerican morning glory):
https://www.shroomery.org/forums/showflat.php/Number/27850299/page/2

Make your own 1-acetaldehyde LSD at home from LSD, very similar to ALD-52 or the real orange sunshine:
https://www.shroomery.org/forums/showflat.php/Number/28441105

On my very first pan cyan mushroom trip, where I went to a house music club tripping with friends, I viewed laser light patterns on the floor of the club, where the women danced, I believe the mushrooms showed me how to form never before seen patterns, as went I went home, over the next several months, I built my own 6 channel audio generator that when these combined frequencies (3 on x channel and 3 on y channel) were sent to a laser x and y galvanometer, were able to produce brand new laser patterns such as collapsing circles and spinning lines 360 degrees which looked beyond belief in the fog as 3-d, I then went on to market these laser scanners to clubs on the strip, and they were a huge success...I owe this creative invention to the mushrooms which sparked new creative energies, way beyond thought, from a higher source where the mushrooms tap into. My love for house music stems back to those days of visiting many clubs as an entertainment laser lighting fixture creator and programmer and making friends with the many DJ's. Over the summer myself and friends were lifeguards at the local water park. But on the weekends we went to parties or house music clubs.

https://www.friskyradio.com/
https://jaytechmusic.podbean.com/
--------------------------------------------------------------------------------------------------------------------------
Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms:

8-8-2023

Step 1: First wipe the black inoculation port on jar lid with alcohol pad and inoculate each of your 24 oz 5 grain jars with around 6cc from estero pan cyan spore syringe for instance.

I'm lazy so I already had bought super cheap pre-sterilized jars of rye berries with 4 other grains mixed in, came pre-sterilized at 15 psi for 120 minutes. When inoculate, spray 2 mm down the middle, then spin jar 1/4 turn as you inoculate around 1 cc each 1/4 turn towards the sides of jar so liquid drips down from top to bottom.

I did not even need to flame the syringe with mini butane torch as the spore syringes already come with a sterile syringe in sterile packing, you just screw on the sterile syringe and inject, no flaming of needle needed. I do all this in front of a laminar flow hood, although you don't need one.

In only 2 days, notice one of the jars in middle already showing mycelium growth. In around 5 days all of the jars SHOULD show signs of growth.


-------------------------------------------------------------------------------------------------------------------------------------------------
8-21-23

Step 2, so after 14 days, only the one jar in the middle showed vigorous estero pan cyan mycelium growth...I even tried inoculating more spore water and even entire print spores into the non responding 5 other jars, but still no response after 2 weeks...As you can see growth may not be smooth, and with this grow log I want to show problems encountered...

...next time I start from scratch, I will use only spore water syringes or spore print spores directly to AGAR PLATE, cut agar wedge and then transfer wedge to grain jars...I will never start from spores dropped into jars or spore syringe water shot into each jar again (as it only worked for 1 of the 6 jars).

So what I did is at day 15, I did a grain to grain (g2g) transfer of all the fast growing estero mycelium from the 1 jar with major growth into each of the other 5 jars, and it took off like wildfire....I did this in front of a flow hood, I simply dipped a clean spoon into a small glass full of 91% isopropyl alcohol to sanitize, held spoon in front of flow hood to dry off, then opened the one jar with 40% growth, grabbed 2 tablespoons of mycelium covered grain, and added this to each of the other 5 jars...it worked!

Pic1: In just one day all of the other 5 jars have taken off very fast and even the main jar with growth now shows growth all over the jar after shaking it to break it all up before the g2g transfer. I shook each of the 5 jars after adding the 2 tablespoons, and it worked very well.

Pic2: Flow hood is nearly 14" wide so that even a $2 dollar cake pan (with clear lid added later) will fit in front of it (followed Gordotek) when transferring the grain spawn in step 3 coming up later to the substrate in cake pan, and mixing all together.

Super easy Gordotek that was followed: https://www.patreon.com/posts/new-simple-tek-44645256?l=es



Edited by tregar (01/13/24 01:38 AM)


Extras: Filter Print Post Top
Offlinenormalperson
Stranger
 User Gallery

Registered: 10/31/19
Posts: 728
Last seen: 18 hours, 36 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 1
    #28442674 - 08/22/23 10:38 AM (5 months, 3 days ago)

seems rather complicated for something described as super easy.  i think i read on this site somewhere that you can grow pans on BRF pucks?  here it is....https://www.shroomery.org/forums/showflat.php/Number/18357343#18357343


Edited by normalperson (08/22/23 11:00 AM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: normalperson]
    #28447122 - 08/26/23 08:06 AM (4 months, 30 days ago)

--> 3 days after g2g (grain to grain) transfer from a single jar, all 6 jars showing very fast growth, zero contamination, each jar is enough to mix into a single 13" x 9" x 2" substrate full cake pan, 3 jars or 3 cake pans are even enough to produce a life time supply of level 5 trips for many people.



Extras: Filter Print Post Top
OfflineRoscoeReturnsS
Crotchety chode man
Male


Registered: 02/12/18
Posts: 1,738
Loc: State of Confusion
Last seen: 2 hours, 50 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 1
    #28447161 - 08/26/23 08:59 AM (4 months, 30 days ago)

:popcorn:


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: RoscoeReturns]
    #28453291 - 08/31/23 08:15 AM (4 months, 25 days ago)

Thanks RoscoeReturns. 4 days later, g2g transfer worked well.



Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28475100 - 09/19/23 02:49 PM (4 months, 6 days ago)

1. Around 1 week later all the grain was colonized

2. Each of 6 cake pans (13 x 9 x 2) had 6 x 1/8" holes drilled, then placed a round sticky Air Filter Vent 0.22 Micron with 3m Backing. This is a small circular filter used for facilitating gas exchange (these come in packs of 50 for around ten dollars).

3. In front of flow hood: Into each cake pan (pre-cleaned with 91% alcohol in front of flow hood and allowed to dry) was placed substrate and all the mycelium grain from a single jar, hands were cleaned beforehand and rinsed & rubbed with 91% isopropyl alcohol, all the substrate and grain was mixed together by hand in the cake hand in front of flow hood, the lid placed onto cake pan, did same for remaining 5 cake pans.

4. Leave these 6 cake pans out on table for around a week or so for substrate to be colonized by the grain mycelium spawn. I like to keep a ceiling fan running on low.

Note: Manure/grass bulk Substrate is made from sun-dried horse manure, extra chopped straw, coir/vermiculite and ph buffers. Then is pasteurized for 2.5 hours in hot water bath. This kills bad bacteria but allows good bacteria to live. You can find precise recipes for this in Gordotek's tek writings.



Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28479408 - 09/23/23 02:03 AM (4 months, 2 days ago)

Unbelievable, after only a few days, a thick layer of pure white mycelium like snow can be observed thru the tops of each tray, eating thru the substrate in record time. Zero contamination.



Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28490738 - 10/03/23 08:23 AM (3 months, 23 days ago)

The temperature during the day was 76 degrees F and at night 71 degrees F, and this is how it appears days later with those temps, very good growth...however, decided to speed the growth up now by placing a heat mat under each of the 3 trays (2 heat mats) and set them to 25 degrees C (78 degrees F), this way at night, the growth will continue to be very fast...using this method, expect to see the trays finish in just a few more days...still zero contamination, all pure white mycelium like snow, ceiling fan above always runs on low.



Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28490742 - 10/03/23 08:33 AM (3 months, 23 days ago)

Gordo is a noob parrot, parroting old ideas while oftentimes outright plagiarizing. His videos are memorials to posterity illustrating his misunderstanding of fundamental principles.

Nice work, Gordo, the crown jewel of noobery.


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: Stipe-n Cap]
    #28490772 - 10/03/23 09:01 AM (3 months, 23 days ago)

I think Gordo is a very intelligent fellow, I'm not gordo, I'm tregar...his method is awesome, that's why I followed it. I know a good thing when I see it.


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 2
    #28490822 - 10/03/23 09:54 AM (3 months, 23 days ago)

Guess what, this isn't his technique. Gordo is a hack noob, that's my point. Anything you saw of value did not originate with him.

All of his content is stolen from old shroomery threads. Very intelligent indeed.👌

He fails to grasp basic techniques because he hasn't taken the time to do anything other than plagiarise and parrot. He's a gawking parrot, the end.

I make an effort to make this sort of statement anytime I see his content, it's what plagiarists deserve.

He states that the application of the casing layer should be done in sterile flow or in a SAB, he goes on to claim that his flush suffered as a result of the application of his casing layer in open air. He has no idea what he's talking about, it's evident with every detail.

Good luck with your grow.


Extras: Filter Print Post Top
OfflineReishiman
Hobby grower
Male User Gallery


Registered: 08/03/23
Posts: 113
Loc: Neither here, nor there
Last seen: 2 hours, 57 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: Stipe-n Cap]
    #28490838 - 10/03/23 10:17 AM (3 months, 23 days ago)

It seems like most of the people making the you tube videos or patreon in his case are more into making videos than growing mushrooms. That being said his videos do seem to provide decent recipes for reference. He seems like he either researched pretty good or maybe has been trained in college but I guess he should provide his sources. I always like to check whatever I see on YouTube on shroomery to see if anyone has anything to say about the given recipe or technique/method. And then apply common sense and see what works for me.


--------------------
Animals are something invented by plants to move seeds around. ... Terrence McKenna

A psychedelic point of view is one that values consciousness... Terrence McKenna


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: Reishiman]
    #28491069 - 10/03/23 02:51 PM (3 months, 23 days ago)

Note: If his (Gordotek) method works, I'll end up with a lifetimes worth of +5 level trips on just a single grow. We shall see.

As a long time chemist, my method of making tetrahydroharmine (see post #1 link) using zinc dust or granules is completely different from his method which uses magnesium which I would never follow. However, as far as I can tell, we are the only 2 people who have posted totally different methods on how to make pure THH.

I also do not smoke or vape dmt as he does in another video he has, as I prefer the oral only method of traditional Ayahuasca, combining it with at least 100mg of pure THH. I've used oral Ayahuasca capsules at least 90 plus times over many years. I march to the beat of my own drum. I don't make videos and don't like Patreon as you have to pay money to join, so there are indeed many things I do very differently.

If you like LSD like I do, combine panaeolus with at least 100mg of the pure betacarboline THH, it's quite incredible, like a long lasting natural version of LSD. I give experiences from others who have tried the same combination in the link in post #1.


Edited by tregar (10/03/23 03:14 PM)


Extras: Filter Print Post Top
Offlinetree frog
eats bugs

Registered: 09/14/23
Posts: 442
Loc: lives in trees
Last seen: 1 hour, 37 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 2
    #28491101 - 10/03/23 03:21 PM (3 months, 23 days ago)

Why 91% rubbing alcohol?

And I don't see why you're inoculating pasteurized substrate in front of a flow hood.

Are you somehow maintaining a sterile environment?  I.e. sterilizing the cake pans and substrate somehow?

Wouldn't using a grow bag for edibles be easier if you want it sterile?  Turn the bag on the side so it replaces the cake pain.  Cut it open and case when colonized?

I wish you luck with your grow but I think there's much easier ways to do things and that, last I knew, 70% Iso was better for mycology as the water helps the alcohol penetrate cell walls.  But maybe there's new information on that I'm not aware of :shrug:


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Extras: Filter Print Post Top
OfflineReishiman
Hobby grower
Male User Gallery


Registered: 08/03/23
Posts: 113
Loc: Neither here, nor there
Last seen: 2 hours, 57 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 1
    #28491125 - 10/03/23 03:36 PM (3 months, 23 days ago)

Quote:

tregar said:
Note: If his (Gordotek) method works, I'll end up with a lifetimes worth of +5 level trips on just a single grow. We shall see.

As a long time chemist, my method of making tetrahydroharmine (see post #1 link) using zinc dust or granules is completely different from his method which uses magnesium which I would never follow. However, as far as I can tell, we are the only 2 people who have posted totally different methods on how to make pure THH.

I also do not smoke or vape dmt as he does in another video he has, as I prefer the oral only method of traditional Ayahuasca, combining it with at least 100mg of pure THH. I've used oral Ayahuasca capsules at least 90 plus times over many years. I march to the beat of my own drum. I don't make videos and don't like Patreon as you have to pay money to join, so there are indeed many things I do very differently.

If you like LSD like I do, combine panaeolus with at least 100mg of the pure betacarboline THH, it's quite incredible, like a long lasting natural version of LSD. I give experiences from others who have tried the same combination in the link in post #1.




Good luck and may you end up with a life time supply of pans. Make sure you look into proper drying and storage so they don't lose potency throughout your life. I'm also trying to grow pans right now for the first time. Got pan Bisporus in grain jars that are almost done colonizing. Also have pan Cambodian that I just some spores on agar but starting to see growth. Hopefully will have some by Christmas time to give to people as gifts. Btw I've been storing my cubes in the freezer in jars with decessent packets in jar with the shrooms in a separate bag but in the same jar as the decessent packet. So far it seems to be working. But you may want to look into it if you haven't already. The shrooms I grew last year went to shit in double plastic zip lock bags...they took in moisture from the outside air and I had to toss them.


--------------------
Animals are something invented by plants to move seeds around. ... Terrence McKenna

A psychedelic point of view is one that values consciousness... Terrence McKenna


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: Reishiman]
    #28496312 - 10/08/23 09:32 AM (3 months, 18 days ago)

Reishiman said:
Quote:

Good luck and may you end up with a life time supply of pans. Make sure you look into proper drying and storage so they don't lose potency throughout your life. I'm also trying to grow pans right now for the first time. Got pan Bisporus in grain jars that are almost done colonizing. Also have pan Cambodian that I just some spores on agar but starting to see growth. Hopefully will have some by Christmas time to give to people as gifts. Btw I've been storing my cubes in the freezer in jars with decessent packets in jar with the shrooms in a separate bag but in the same jar as the decessent packet. So far it seems to be working. But you may want to look into it if you haven't already. The shrooms I grew last year went to shit in double plastic zip lock bags...they took in moisture from the outside air and I had to toss them.


Thank you kindly for tips Reishiman.

Gordotek said:
Quote:

The "Estero" strain was the best strain that I tested until finding TTBVI which is even better (just as aggressive and easy to grow but even more potent and it prints better). They are extremely vigorous, potent, and produce high yields.  There was some doubt at one point that the Estero strain was even pan cyan, some thought it might be pan cambo because it was almost too easy to grow.  However thin layer chromatography clearly shows it is not pan cambo, it has the alkaloid potency in the HIGH end of reported ranges for pan cyan. 

Comparison of Estero (Pan cyan) and Albino Penis Envy (Cubensis) mushrooms via Thin Layer Chromatography: https://www.patreon.com/posts/67621105

I will create a video explaining how to do this type of analysis, but for now I just wanted to show everyone a comparison between these species.  Albino Penis Envy (APE) is widely considered to be one of the most potent cubensis cultivars, it was heavily represented in the latest psilocybin cup (if you want to see my comments/critique of the cup, click the link and scroll down to the bottom where comments are shown, to see comments on a phone you may have to tap settings (in chrome for example)->desktop view).

So how does APE stack up against Estero (pan cyan)?

Each dot in the picture above represents a chemical compound (these are labeled on the left). They are in two vertical columns, so the left side from top to bottom is all Estero, right side is APE.  Starting with the two most important, look at the psilocybin and psilocin content - quantification is not precise, but you can estimate based on spot size and darkness.

The Estero cultivar is approximately three times as potent as APE (and it would have easily blown all other entries out of the water had it been submitted to the psilocybin cup).  This should be a reminder to all to dose appropriately (pan cyan holds the record for most potent mushroom species in the world as per published research).

If you normally take 3 grams of cubensis, try 1g of Estero.  It is always better to start with a lower dose and see how you respond before trying anything higher (you may want to start with just 0.5g).  There is NO PRIZE for taking the highest dose, in fact there is no documented benefit of any dose beyond about 35mg psilocybin equivalent, just more fear & anxiety for most people.

But there's more to it than just triple the psilocybin and psilocin, Estero also contains baeocystin (hard to see, but its there), urea (quite a bit), and serotonin.  None of these compounds can be seen at all in APE.  Could these be partly responsible for the difference in anecdotes from user experiences on Estero?

Here are some comments people have sent on this subject:
 
    "I strongly agree with starting with one-half gram dry.  It was a much better experience than any cubes I've tried even at higher doses."
 
    "Cubes gave me mild nausea and made me feel 'shakey'.  Not at all so with Pan cyans"
 
    "Penis Envy is and has been overrated. I think any other well sourced Cube is just the same. It’s that cultural conditioning at play."
 
    "lol who needs cubes when you can have Cyanescens!  I’ve blessed a few people with them and they are now believers lol."
 
    "When Estero kicked in, I wasn’t disappointed.  At one point I forgot I ate Cyanescens and thought I was on a DMT ride!  As often happens to me on DMT I was 'connected' to some kind of power cord or machine umbilical that was coming from my wall straight towards me and plugged into my third eye!  And I felt the presence of a cosmic nurse at my side. Like she had her hand in my shoulder reassuring me all was ok!  Then I was like “f*** I ate mushrooms this ain’t a DMT trip” :wink:
 
    "I've taken enough cubes to know Estero is different... 2 grams. Most amazing experience of my life. 10 hours. And another two hours of happiness watching TV and talking about my experience with my wife. In the end I just want to thank you. No comparison."
 
    "OMG, OMG, O. M. G. Had my first deeply religious experience on 2g [estero] yesterday. I have never felt anything like it in my life. It was truly incredible."
 
    "I ended up taking a 1 gram of Estero since I’m used to taking 3-4 grams of cubensis. Man…you were not kidding about the potency of these. 1 gram easily felt like 4-4.5 grams of cubensis to me.  These were by far the best visuals on mushrooms I’ve ever had.  Also, the euphoria was quite intense, and giggle sessions were uncontrollable.  All in all, I love pan cyans and they’ll likely be my go to. I’ll likely still grow cubensis for microdosing as well as less potency for sharing with others."
 
    "Experienced people do sometimes doubt ones sanity trying to explain just how deep Estero gets on what some call a "micro dose" the pan experience for me is the most rewarding substance I've ever ingested and half reduced my dose and frequency of use to fine tune the benefits, plus stopped using synthetic drugs completely and have been experiencing recovery i didn't really know I need but pretty sure i do."
 
    "I didn't really expect how good the differences were from Cube to estero. The visuals weren't comparable at all and the euphoria was so wonderful. My 2g trip was literally the best substance experience of my life next to marriage and kids. 10 hours of pure bliss." [Gordo note: 2g is too much for most people, be cautious]
 
    "Funny because I went to an Ayahuasca ceremony in May and the Shaman told me to start growing pans... he said, “Cubes are for the people. Pans are for the elevated.”
 
    "First experience last night with Estero pan cyan. Truly mystical, amazing, and the most beautiful and gentle psychedelic experience to date. Truly a gift."
 
    "There's something about Pan Cyan that induces something special that no cube could ever bring, it isn't that it's a clean feeling either - it's something that must deal with happiness in general - so whether it stimulates serotonin production or a mixture of neurochemicals for the best experience, I have had consistently positive experiences with each trip both physiologically and psychologically. I wonder if it has to do with other alkaloids or absence of alkaloids vs cubensis"

One of this years funniest/best psychedelic reveals was the guy who actually came up with Penis Envy (the best selling cubensis cultivar of all time), when interviewed by Hamilton Morris essentially said (my paraphrase) 'Eh, it's still just a cube, and cubes don't do it for me. I find the Cyanescens far superior (they have entities)!'

Haha. So the guy who created the most popular cubensis cultivar in the world doesn't even like cubensis.  Eventually I think most in the grower community will come to prefer other species.  I would still like to see some reputable published research comparing user experience by species (this is challenging to do but worth a try).  As an aside, if you listen to that Hamilton interview, the PE guy says a lot of "out there" things that deserve to be challenged, every so called claim of "supernatural powers" has always failed rigorous testing and he is no exception.




Since the temp at night was 70 degree F, the heating mat did the trick, after 3 days each of the 6 cake pans had completed snow white mycelium coverings, kept each heating mat at around 76 to 78 degrees F. It has auto-shutoff once temp goes above 77 degree in room. These programmable heat mats even come in packs of 4 for dirt cheap combo.

The Estero variety panaeolus (native to Florida) seems to prefer a constant 77 degree F. Temps between 70 to 85 degrees F work well.

1. This is how each of the 6 cake pans looked before I took the cover off in front of flow hood. Notice the tiny indoor humidity and temperature monitor placed on edge on top of each heat mat to monitor the programmed temperature, to make sure it was working.


2. Even though I used 2 mycobags full of casing dry mix, turns out I only needed one bag's worth in order to cover each of the 6 cake pans with a 1/4" layer. The casing dry mix is a proprietary blend of peat moss, vermiculite, gypsum calcium phosphate that was bought dirt cheap. The exact recipe is given in Gordotek's writings: https://www.patreon.com/posts/44645256


3. A 23 qt presto pressure canner was used to sterilize the soil at 15 psi for 45 minutes. It's very easy to use a canner, all you need to know comes with the instructions with the canner.

1. When you get your canner, just screw on the dial pressure gauge that comes with it by hand, put tray into canner that sits around 1/2" above the bottom, add 3 quarts of hot water to the pressure canner, put your mycobags full of soil into canner (make sure you have a couple clothespins holding it flapped over shut), put lid on canner and rotate it to closed, heat up canner on "8" or so stove setting. Watch air steam vent out of the open vent pipe for 10 minutes. Then put the pressure regulator on the vent pipe, the pressure regulator may begin to rock before pressure builds. Watch as pressure builds in canner, once the dial indicates you have between 10 to 15 psi, turn down the heat to "6" or medium or so, and allow canner to PC cook for 45 minutes. The bags can withstand the temperature no problem. They will not melt. Do not remove the pressure regulator from the vent pipe until the pressure has gone back down to zero.



4. Cooker was removed from burner and allowed to cool overnight. Lid was removed from cooker. This is how the 2 casing bags looked the next day inside the cooker.


5. The lid was removed from each cake pan in front of the flow hood.


6. This is a closeup of one of the cake pans.


7. With clean hands (pre rinsed with 91% isopropyl alcohol, and hands allowed to dry quickly in front of flow hood, the mycobag was held over the cake pan, easily eye'd an amount of casing out over the cake pan so that a 1/4" layer was spread out in a smooth even layer. I lightly tapped the layer down and sprayed it heavily with distilled water after it was in place.


8. This is pic of all 6 cake pans after casing layer was placed. Optionally I put the top back on each cake pan (the inside of the lids were pre-wiped with 91% isopropyl alcohol and allowed to quickly dry).

The lids are being put on just for 24 hours, the mycelia needs to poke through the casing just a bit before pins will form and keeping the top on seems to speed the initial move up, probably because it keeps the casing layer warmer. This may be helpful especially if your grow area is colder than 70F.


Extras: Filter Print Post Top
Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
Loc: lives in trees
Last seen: 1 hour, 37 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 2
    #28496322 - 10/08/23 09:40 AM (3 months, 18 days ago)

The sterile technique is interesting.  I have some polypropylene take out containers (lids are PP too) I will have to try this with.

Pretty sure 70% IPA is better though, regardless of Gordo's tek.

https://www.shroomery.org/forums/showflat.php/Number/14455047


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tree frog]
    #28496351 - 10/08/23 10:09 AM (3 months, 18 days ago)

tree frog said:
Quote:

Why 91% rubbing alcohol?

And I don't see why you're inoculating pasteurized substrate in front of a flow hood.

Are you somehow maintaining a sterile environment?  I.e. sterilizing the cake pans and substrate somehow?

Wouldn't using a grow bag for edibles be easier if you want it sterile?  Turn the bag on the side so it replaces the cake pain.  Cut it open and case when colonized?

I wish you luck with your grow but I think there's much easier ways to do things and that, last I knew, 70% Iso was better for mycology as the water helps the alcohol penetrate cell walls.  But maybe there's new information on that I'm not aware of :shrug:

The sterile technique is interesting.  I have some polypropylene take out containers (lids are PP too) I will have to try this with.

Pretty sure 70% IPA is better though, regardless of Gordo's tek.

https://www.shroomery.org/forums/showflat.php/Number/14455047


Hi treefrog, thanks for watching. I'm just following the steps as given in Gordotek, sure you could use mycobags to grow out your grain mixed with substrate, sealed with impulse sealer. This is what gordotek had to say about those things:

Gordotek said:
Quote:

Transfer your substrate into the cake pans, only fill to 3/4 full.  Straw based substrate is more prone to contamination than a coir based substrate, if you have a flow hood do this step in front of it, or use a still air box, otherwise you can try doing this in open air which actually worked fine for me or optionally even try doing everything under a clear plastic drop cloth. Thoroughly clean and sanitize a table, put your cake pans, grain spawn, isoproply alcohol spray bottle, scissors, and substrate on the table.  Spray down everything with isopropyl alcohol.



Gordotek said:
Quote:

Note: An alternative to the above is to add your grain spawn directly to the mycobag after it is cooled and seal the mycobag, shake and let it colonize there for a week before transferring it to your cake pans - I tried this and it worked but cost an extra week of time as it will have to recolonize for another week after you transfer it to the cake pans.  I would only recommend this if you are having problems with contamination. To me it was a waste of time.




Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tree frog] * 2
    #28496353 - 10/08/23 10:09 AM (3 months, 18 days ago)

He's "a long time chemist", I'm sure he knows that disinfecting efficacy drops off at concentrations higher than 80%, and why. Anybody can grow mushrooms, Gordo included, even if they don't really know how or why they're getting them, or the how and why most of the process works.

To follow a recipe you don't need to understand why the recipe is prescribed to begin with.


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: Stipe-n Cap]
    #28496597 - 10/08/23 02:11 PM (3 months, 18 days ago)

Maybe there is some confusion, I only use the 91% isopropyl alcohol to rinse my hands and let dry quickly in front of flow hood before pouring casing over cake pans, patting down casing, etc.

Phase II:

There is little chance of contamination once the casing layer is on as Gordotek states. After 24 hours, I will remove the lids, and start the humidifier timer cycle.

1) Gather materials: Spray water bottle, monsoon humidifier with stretchable hose, a few humidity/temp monitors, mechanical timer.


2) Instructions for adding water to humidifier:


3) Program timer to turn humidifier on every 1/2 hour for 1/2 hour by first lifting every black button, then pressing down every other button.


4) All cake pans can be put into an empty kiddy swimming pool in a room to create a "sink/catch" for the fog or into a spare bathtub, we want to make a makeshift "tub/pool". First put down the programmable heat mats, set temp to around 76 degree F, place a humidity/temp monitor on each mat, then put cake trays on the mats, pre-spray the tub with lysol, clean well in advance.


Since my spouse will be using the mushrooms at threshold doses to treat her migraines (hopefully they will eliminate her migraines for weeks at a time, as she now gets them daily or every few days due to an ear infection back in June that finally went away months later but left here with this current head condition), she let me use a spare bath tub. 

Gordotek said
Quote:

If outside temps are 70F to 85F you can open a window. Some growers like to have additional fans running for more circulation but I found this to be unnecessary, the humidifier itself creates nice air currents that stream over the surface of the blocks and this is all you need. I did not use electric light, just natural indirect sunlight from the windows in the room, but if your room has no light, you can use 12 hour on, 12 hour off lighting. You could put the light on a time but since you will be adding water to a humidifier once a day, it's probably just as easy to turn the light on and add the water every morning when you wake up and turn the light off when you go to bed.

Set the humidifier at the top edge of your "pool/tub" elevated, so it throws mist out and forward into your tub. It should NOT be on the highest setting, a mid range setting may be fine, you want it to be throwing out some serious fog but not filling the entire room with fog. If you use a hygrometer near your tub, it's going to go up to about 95% RH (don't leave your hygrometer there too long or it will likely fail).  Plug the humidifier into a timer so that it goes on for 30 minutes then off for 30 minutes, around the clock (so it will be on for 12 hours a day total, every day).

At this point, you just need to monitor it a little bit for the first day to make sure you don't have the humidity setting too high or too low, Ideal humidity is 95%. You don't want pools of water forming, just a nice fog/mist overy your cake pans. If you get one of the high capacity humidifiers I recommend, you will only need to add water once a day. From that point on, it should be "set it and forget it". You should see pins form in 5 to 7 days. If substrate looks dry, you can mist with water. Pick the mushrooms as they mature, dry them in a food dehydrator and enjoy!

this species tend to go into almost "continuous fruiting mode" with wave after wave of mushrooms. You should get pretty thick carpets of mushrooms using this TEK, some flushes will be thicker than others.

With flush after flush, a single grow could produce a lifetime supply for some people.  A good strain can produce a crop that is strong enough to hold up a cell phone.

These can be harvested by cutting at the base with a sharp knife OR by gently twisting and pulling them out but there will usually be pins for the next crop all over the surface so try to avoid damaging those (I prefer using a long sharp knife). 

After a harvest pour 1 cup of water as evenly as possible over the entire surface of the pan. It’s going to look like an excessive amount of water, but it’s not.  At any given time, if it looked a little dry I would also spray the surface with a spray bottle of tap water (dollar store spray bottle is fine). The rumors that spraying water directly onto pins will kill them or cause them to abort have been debunked (by me). I have sprayed around, and even directly on pins just to see what would happen, and it didn't cause any problems, I have also directly sprayed the mushroom fruit bodies to see what would happen, it didn't make any difference. Remember how much this species loves rain.  That said, I would try to avoid directly watering the mature mushrooms, excessive humidity can cause the caps to turn dark, as can low grow room temperatures.

Dry in a dehydrator, homogenize by blending, and pack into 00 capsules using a capsule maker tool as shown in my bulk mushroom grow video.  Store in a sealed glass jar, at room temp, in a dark place preferable with oxygen absorbers. These are typically 3 times as potent as cubensis, so take 1/3 less. A single capsule (0.5g) is a good starter/test dose to get a feel for the species. A sensitive person might have 2 hours of closed eye visuals on such a dose. One gram (two capsules) is considered a normal dose similar to a 3 gram trip with cubensis and 1.5g (3-4 capsules) is a high dose.  2-3 grams could be very unpleasant for many people - you have been warned!  Use moderation, homogenize your dose, and only increment in small (half gram) steps after trying a lower dose first.





Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28496748 - 10/08/23 04:27 PM (3 months, 18 days ago)

5) In operation: Humidifier quickly achieved a 98% humidity set to "high range" so turned it down to "low range" via it's dial, timed to be on every 1/2 hour for 1/2 hour, 1/2 hour off. It's cool to view the fog flow all around the tub above the cakes, hovering and moving in a counterclockwise direction across the whole tub.

Placed a drape across the front of bath tub using a thumbtack on either side, and left one far side open an inch to allow air flow, and kept the overhead bathroom vent on all the time, although this does not probably matter, on or off probably no difference. A/C vent in ceiling provides fresh air all day long, and keep the bathroom door closed or cracked an inch, you decide.



Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 1
    #28497529 - 10/09/23 11:45 AM (3 months, 17 days ago)

Let me save you a little headache while we're on the subject. You don't need a flowhood to apply any casing layer, ever, for any reason.

I'll let you figure the rest out on your own.


Extras: Filter Print Post Top
Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
Loc: lives in trees
Last seen: 1 hour, 37 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: Stipe-n Cap] * 1
    #28497680 - 10/09/23 01:50 PM (3 months, 17 days ago)

You lose all beneficial microbes by sterilizing too.  Which will protect the casing from contamination and possibly form symbiotic relationships with the fungi.

Pasteurization I think is better.  And you wiped the inside of the lids with 91% IPA, shit is already unsterile.

Not because you used the worse IPA (though that too) but because sanitation isn't sterile.  Also you sprayed with distilled water.  Was the bottle sterile?

See what I'm getting at?

I'm sincerely glad your grow is working, but it's growing inspite of flaws in your technique.  And would possibly do better with better procedures and a better understanding of why things are being done and what they do and do not accomplish.

Honestly go read some of the poster aboves techs, they're maticulous, and explain why all the meticulous shit.  This tek you're posting is needlessly complex and seems to have a lot of dubious techniques.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Edited by tree frog (10/09/23 02:05 PM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tree frog]
    #28500609 - 10/11/23 07:13 PM (3 months, 15 days ago)

I totally agree with you treefrog....if I were to do this over again, I would have definitely used mycobags and an impulse sealer (well worth the cost) to mix the grain with the substrate...then when it's all grown out, simply break open the bag and crumble it into a fresh clean tray in front of flow hood and add the 1/4" sterile casing. So what if it takes an extra week to colonize...

...as I lost a container due to contamination...this would not have happened with the mycobags mix.

I cut around the contamination outside which was centered in one area, and broke up the rest and put it in an outdoor garden container with a 1/2" left over sterile substrate to fend for itself, luckily the humidity is perfect 95% degres outside after a rain, and 76 degrees F. So who knows....


Extras: Filter Print Post Top
Offlinenormalperson
Stranger
 User Gallery

Registered: 10/31/19
Posts: 728
Last seen: 18 hours, 36 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28501014 - 10/11/23 11:01 PM (3 months, 15 days ago)

So........ still no shrooms to harvest?


Extras: Filter Print Post Top
Offlinemed man
A equal opportunity offender

Registered: 03/13/23
Posts: 185
Loc: The 3rd coast
Last seen: 4 days, 18 hours
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: normalperson]
    #28501169 - 10/12/23 04:44 AM (3 months, 14 days ago)

Quote:

normalperson said:
So........ still no shrooms to harvest?





:whathesaid:


--------------------
Somewhere between chaos and disorder.


Extras: Filter Print Post Top
Offlinethe_prodigy
Be water my friend

Registered: 11/08/22
Posts: 23
Last seen: 8 hours, 40 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 1
    #28501301 - 10/12/23 07:56 AM (3 months, 14 days ago)

Quote:


5. The lid was removed from each cake pan in front of the flow hood.


6. This is a closeup of one of the cake pans.





If you lost a tray, I would bet on this one. It looks a bit suspect to me for a pan tray, with those yellow parts on top right and random greyish/dark spots on bottom right and upper left part of tray.

What's up with rest of the trays? Any pinning or grow?


--------------------


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: the_prodigy]
    #28506874 - 10/16/23 01:17 PM (3 months, 10 days ago)

Yes the_prodigy, you are correct, that was the tray I had to throw out.

For the tray I threw out due to contamination a week ago, I cut away all the good substrate from around the contamination and first put a layer of wet perlite into a garden container outside, then crumbled the good substrate on top the wet perlite, and added a 1/4" layer of left over sterile casing soil on top...this is all growing outside, I water it daily lightly with a watering can, and the temps and humidity are actually perfect outside except for night when it can dip. This I learned how to do for an outdoor grow from the 1st paragraph of this link: https://www.shroomery.org/8421/Panaeolus-cyanescens-FAQ





1st pic above several days after moving into humidified bathtub (1/2 hour on, 1/2 hour off) the mycelium can be seen tunneling it's way to the top of the casing.

2nd pic pinheads are forming all over the place, closeup of a few of them.

Soon, will be moving the trays into a Martha tent ecosphere that was given to me by a dear friend who is moving to another state into a smaller place, what a surprise, as this will come in real handy, this way the bathroom can be freed up, and the tent will be able to maintain a constant 77 degrees to 80 degrees, however I set it, due to it having a bulb on the bottom which is a regular old infrared, heat emitting bulb for reptiles. It screws into the power source, and sit's under a tray full of lava rocks. A programmable thermostat regulates the bulb for the perfect set temp.

Even currently with a heater in the bathroom set to go off to maintain 80 degrees F, I just can't get the temp to go over 73 degrees F most of the time near the trays, except for the afternoon when it's 76 degrees, at night the temp can fall low to 70 degrees (and the heater is turned off so no fire hazard) and the heating pads I'm getting rid of too.

The martha tent with the reptile lamp at bottom is definitely an innovative alternative to a ceramic/coil space heater which almost always gets too hot and throws a stream of heat, where as with this the lava rocks diffuse it. That is the main function of the lava rocks, kinda like perlite with it's wicking properties.

The monsoon humidifier I have will be put to good use to throw out humidity from the top downwards, still going on every 1/2 hour for 1/2 hour then shutting off for 1/2 hour and repeat like this all day...and there is a 5" fan at bottom to push air, and large holes covered with replaceable large peel and stick micron filter patches to allow air exchange in several places all over tent. A drip tray that sits under the martha collects any excess water running down the tent. A problem noticed with the bathtub is that water likes to collect in tiny pools here and there.

Evaporation is taking place every 1/2 hour when the humidifier is off for 1/2 hour, and this is important as Jakeoncid419 saids below, as it's just not the temp but the evaporation that makes the difference in how good your pin set will be.

Jakeoncid49: https://www.shroomery.org/forums/showflat.php/Number/26235096/fpart/1/vc/1

Mary Fairchild: https://www.shroomery.org/forums/showflat.php/Number/25248773

Jakeoncid419 said:
Quote:

I like high 70s low 80s you can take it up higher though but the warmer your take it the more likely you are to contaminate however they do move faster peeking out in the upper 90s where are you will start hurting your fruits I like 82 Fahrenheit.

Evaporation is what is going to induce pin formation evaporation takes place more at warmer temperatures I found they do best 80 to 82°F but it’s not just the temperature it’s the evaporation that makes the difference in how good your pin set will be.



As mentioned earlier, one mistake I made was not mixing my grown out grain into the substrate in mycobags in front of flow hood, sealing them, and letting them grow out 100% in a sterile environment like the mycobag...I noticed that when I put the trays into the fruiting chamber, they were maybe only about 75% colonized, which is why it's taking so long for the pins to form...as the mycelium needs to first colonize the substrate fully, that way it's not still doing it when you case the trays, as the pins are only going to form once the substrate is fully colonized.

Here's a pic of how I should have poured my shook up grain into the substrate loaded into mycobags: https://www.shroomery.org/8695/Making-Panaeolus-Substrate-Pictorial Then should have sealed it with an impulse sealer in front of flow hood, let it grow out 100%, and then cut open the bag after it's colonized (in about 7 days) and crumble the substrate into the trays (all in front of flow hood), and then allow another week for the substrate to recolonize or recover, then case the trays with 1/4" sterile casing in front of flow hood, spray down casing heavily, put lids which have the 6 x 1/8" breathing holes on tray for 24 hours, then remove lid and move all trays to fruiting chamber.

Spent a good day reading thru all the teks I posted here on post #1 from all the other experienced pan growers to learn all I could: https://www.shroomery.org/forums/showflat.php/Number/28108398/page/1


Extras: Filter Print Post Top
Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
Loc: lives in trees
Last seen: 1 hour, 37 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28506965 - 10/16/23 02:24 PM (3 months, 10 days ago)

Glad you got pins coming!

And thanks for the links to the marth set ups.  I'm planning to grow pan bisporus in my mylar grow tent downstairs.  It's not temperature controlled, just an ultrasonic and a good light.

I rotate what I grow down there based on the time of year.  So, I won't be trying pans in the tent until late spring or summer.  Will probably try some tubs in the meantime so I can clone something and have good genetics for the tent.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tree frog]
    #28511393 - 10/20/23 06:35 AM (3 months, 6 days ago)

Than you treefrog. Good luck with ur pan bisphorus grow in ur tent downstairs, sounds amazing, please link to it here if u decide in future to share story.

I ended up tossing the rest of the trays this morning as the contamination spread in the fruiting chamber, this is what happens when u don't grow ur substrate out in mycobags and when u don't let the substrate mycelium grow out 100 percent.

I'm starting all over again, gordotek has some obvious flaws to his method. Look forward to using the Martha ecosphere, have it now, and will begin with agar this time. Will put the tent to use by letting the grain jars incubate at optimum temp in the tent.


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28517455 - 10/25/23 08:01 AM (3 months, 1 day ago)

Moonyham said:
Quote:

Sorry I didn't have time to read everything but why the particular mushroom strain? Would this not also work with cubes, subs or other more available mushrooms?



Hi moonyham, this is with the pan cyan grass/dung lovers strain, long considered the crown jewel of mushrooms, slightly harder than cubensis to grow, and the trips are way more visual, very euphoric, very intricate fractal and geometric visuals like acid with saturated tropical colors. Several of my indoor containers contaminated as I did not use mycobags to grow out the substrate...I followed a tek where they were not used, now I know better next time to use them.

More on this variety with 50 trip reports: https://www.shroomery.org/forums/showflat.php/Number/28108398/fpart/1/vc/1

Modern research has determined that the pan cyan strain of mushrooms with their HIGH BAEOCYSTIN and related analogues content hit the adrenal receptors with as much force as MESCALINE or DMT, see chart below. Notice how just plain psilocin alone hits the 3 adrenal receptors (responsible for enhanced colors, euphoria, beauty and music enhancement) with lighter activity.



Attached: Structure–Activity Relationships for Psilocybin, Baeocystin, Aeruginascin, and Related Analogues to Produce Pharmacological Effects in Mice, ACS Pharmacol. Transl. Sci. 2022, 5, 11, 1181–1196:

https://pubs.acs.org/doi/10.1021/acsptsci.2c00177

by Grant C. Glatfelter*, Eline Pottie, John S. Partilla, Alexander M. Sherwood, Kristi Kaylo, Duyen N. K. Pham, Marilyn Naeem, Grant C. Glatfelter*, Eline Pottie, John S. Partilla, Alexander M. Sherwood, Kristi Kaylo, Duyen N. K. Pham, Marilyn Naeem, Vamshikrishna Reddy Sammeta Vamshikrishna Reddy Sammeta, Stacie DeBoer, James A. Golen, Elliott B. Hulley, Christophe P. Stove, Andrew R. Chadeayne, David R. Manke, and Michael H. Baumann.
------------------------------------------------------------------------------------------
Outdoor panaeolus cyanescens strain estero grow looks promising:

Of the 5 trays in the indoor grow bathtub, 2 of the containers did not contaminate, so 5 days ago, I took the two good containers outside, used a clean fork to scrape off the top layer of casing, and crumbled up the good substrate into two outdoor garden containers, I threw out what was in the outdoor container before, cleaned the inside of containers out well with a garden hose...

...and then added a 3" layer of wet perlite (for constant humidity) to the very bottom (as I did not have enough before) then crumbled in the good substrate with clean sanitized spoon, added a 1/4" layer of sterile casing to the top, and gave it a good watering with a watering can to soak the contents of the entire container, each day in morning I lightly water the container's top casing layer for around 1 second with a nearby watering can...if it ever gets super dry I'll give it a longer soak.

...to my surprise 5 days later there is pure white mycelium growing thru the casing layer! It's looks as if I was able salvage two of the containers as they are now growing well in the outdoor containers...the weather is perfect, between 73 and 85 during day, kept in shaded area, it rained yesterday with perfect 76 degree weather all day, and humidity varies between medium to high daily. 

So it appears as if all is not lost...



What's even more amazing is that when I remember crumbling the substrate into the outdoor containers, only about 50% of the substrate was even grown out before, so somehow, outdoors the rest of the substrate was able to fully colonize in just 5 days, otherwise it would not be currently growing out or "tunneling thru" the casing layer, as this does not normally happen unless the substrate is fully colonized below. 

I have lots of outdoor containers of the same color, it's very rewarding to be able to grow outside, and since I live in a state where these naturally grow outside, I will continue to grow like this, it's just plain fun.



Edited by tregar (10/25/23 12:48 PM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28523094 - 10/30/23 07:15 AM (2 months, 27 days ago)

Timestop413 said:
Quote:

I'm not sure the reasoning why but I have had varied success following a similar method to Gordotek where I mix colonized grain spawn with pasteurized substrate.  Despite grain spawn jars looking and smelling clean, when things have contaminated, they've either contaminated after casing (trichoderma) or prior to casing (when I would go to case, I could smell bacteria which I assume is set to fail eventually If I smell that).  I don't know where the vector of contamination is.

Other times I've been perfectly fine it seems without using the myco bag method(instead I did what you did here and just spawned colonized grain to prepared substrate in front of flow hood) which makes me think its either my grain spawn or possibly substrate not being pasteurized properly when things contaminated but I dont know.

I built a pasteurizer using an 50 quart cooler and I try to get the center of my substrate to around 160 for 2 hours. I let them cool and sit for 2-3 days before I use it usually.

I'm experimenting further around the subject of trays going bacterial.  I'm wondering if It may be due to not enough air during colonization period once I mix spawn with substrate.  The reason I'm wondering this, is some of my trays that have had very little room left in them between the sub surface and the lid once filled, have mostly gone bacterial so I wonder if it's anaerobic in these particular situations.  I've put a few small holes on the lids with a layer of micropore or I've also used the "takeout" trays with no holes and tried to leave the lid loose but I don't think it allows air to flow. So I filled some trays less and tried not to pack the substrate down very much.

I see you have plenty of room between sub surface and your lid tops with air filters on them.

I will try colonizing in mycobags.  I've been really perplexed why some trays have contaminated despite the spawn smelling and looking normal, it is disappointing when it happens.



So good to hear from you my dear friend Timestop413, your comments are much appreciated! I picked up a 50 pack of the good unicorn spawn bags with filter patch. I believe that one of the sources of my contamination was early on when I was mixing the grain with the bulk substrate, I remember NOT cleaning the grain jar with rubbing alchohol on outside, and I had touched the outer surface of the jar many times at the same time I mixed the grain with the substrate, introducing a whole load of contaminants into the bulk substrate, a big no no. Like you I look forward to colonizing in mycobags this time, thanks for sharing your grow story, so cool to see you giving this a shot as well.



Started over, doing this the right way, 2nd time is a charm.

1. Under flow hood, for 1/2 half the agar plates, shot 1 cc of estero pan cyan into each plate, visible spores all thru plate, sealed with parafilm. For the other 5 plates, scraped spores from estero spore print into dish using a disposable, sterile scalpels (10 for cheap), all done in front of flow hood.

2. The mushroom ecosphere greenhouse took only 2 hours to assemble, complete dream come true, set the temp you want for inside the greenhouse, and the martha tent does the rest, notice heat source at bottom with lava rocks to diffuse the heat, and fan next to it to act as a convection oven, fresh warm air being sent upwards, fresh air flow is key. Notice polyfil filter discs attached 4" above each shelf, centered on tent, 4 on each side, and 1 on front, 1 on back of tent.


Extras: Filter Print Post Top
Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
Loc: lives in trees
Last seen: 1 hour, 37 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 1
    #28523111 - 10/30/23 07:34 AM (2 months, 27 days ago)

Sorry you lost your grow tregar.

I was rooting for you.

I think if you're growing in sterile myco bags this time and inoculate under a flowhood you'll have a lot more success.

I grow a lot of edibles in bags and it's really as simple as sterilize bag, sterilize grain, inoculate bag with sterile spawn in front of hood, seal, check every few days until colonized.  Cut open, fruit.

With Pan's you can open the bag and case right in the bag, but the substrate might be a little deep like that.  Remember, pasteuirized casing is better than sterile.  Someone said they were pasteurizing small amounts of casing in their dehydrator (since it runs at 160 anyway) in ziplock bags if you're looking for a simple tek on that.  Maybe the mycobags could be broken up into a few totes if they're too deep.

I think Blue Helix had a lot of cool Pan grows in bags.  Maybe someone else can chime in.  I'm just getting into Pans.  Have two cultures from MS on T1 plates just now that I'm going to play with over the winter.

I'll probably do shoeboxes in my tent.  Straw, manure, coir or some mixture of them.  Pasteuirize everything.  Pseudo-case with coir.  Fruit at spawn like Baba Yaga did in a few of his monotub experiments.  Goal is to clone a culture that will fruit well with minimal effort.  If my first grows suck but I get the clone I want from it, it's a success imo.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Extras: Filter Print Post Top
InvisibleStipe-n CapMDiscord
Male User Gallery

Registered: 08/04/12
Posts: 7,623
Loc: Canada
Trusted Cultivator
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28523380 - 10/30/23 12:51 PM (2 months, 27 days ago)

It sounds like you need to familiarize yourself with basic cultivation techniques, and stop relying on unreliable sources like Gordo. I said this from the beginning, it has unfortunately ended as I suspected it would.

Check out some of our own subject materials:


https://www.shroomery.org/forums/showflat.php/Number/22216410

https://www.shroomery.org/forums/showflat.php/Number/25925194/vc/1


https://www.shroomery.org/forums/showflat.php/Number/26565367

That should help.


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: Stipe-n Cap]
    #28527553 - 11/03/23 07:33 AM (2 months, 23 days ago)

treefrog said:
Quote:

I think if you're growing in sterile myco bags this time and inoculate under a flowhood you'll have a lot more success.


Thanks treefrog, exactly what I'll be doing this time.


stipe n cap said:
Quote:

It sounds like you need to familiarize yourself with basic cultivation techniques, and stop relying on unreliable sources like Gordo. I said this from the beginning, it has unfortunately ended as I suspected it would.

Check out some of our own subject materials:

https://www.shroomery.org/forums/showflat.php/Number/22216410

https://www.shroomery.org/forums/showflat.php/Number/25925194/vc/1


https://www.shroomery.org/forums/showflat.php/Number/26565367

That should help.



Thank you stipe  n cap. I went back and read just about all the links I posted from all the experienced growers on page 1 of the 50 pan cyan experiences thread, esp from Jock and Asura and Blue Helix and Baba yaga. I'll check out all 3 links you posted here too. I'm no longer following the Gordotek method, learned the hard way on 1st grow it's not for me, too unsterile.

5 days later: The 5 agar plates that had spores scraped into them from off an estero pan cyan spore print using a sterile scalpel to scrape them off in front of a flow hood all show wonderful growth, around a 1/2" or more growth of mycelium in a circle surrounds all the spore deposit spots.

The lava rock heater works great without any water put into the rock bin. I found when water is put in it just creates humidity in the tent, not necessary during the agar or grain grow. It can be 50 degrees outside and 65 to 70 degree F in house, and yet the temp controller can be set to 77 degrees F, and the 77 degree F heating is maintained all day and night.

When the agar plates are completely grown out several days from now, will cut several wedges from each plate using sterile disposable scalpel and drop them into a sterile grain jar by removing lid in front of flow hood, and allow the grain to grow out. Have 6 sterile grain jars ready to accept wedges when ready.

Asura said:
Quote:

Pan cyans like temps in the 76-80°F range. If I could lock in a temp perfectly, I would have it exactly 77°F at all times. A little fluctuation doesn't seem to hurt, but if temps drop too low, especially before the first flush, it can ruin the entire grow. Drops can even happen in the middle of summer in Texas. This is why I have a heater in my grow room year around.




https://www.shroomery.org/forums/showflat.php/Number/25987049


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28533918 - 11/08/23 08:05 AM (2 months, 18 days ago)

(1) I dropped 3 agar wedges into each of the 6 grain jars, and the mycelium 3 days later hopped off the agar and onto the grain, pure white mycelium now growing on the grain. This will be a separate grow along side a grow identical to Asura's below:

(2) I decided to follow Asura's "cultivating panaeolus cyanescens" word for word for the main 2nd grow consisting of two large unicorn mycobags full of substrate/supplemental grain.

I want to compare the two grows side by side. In case one fails, hopefully the other will make it to the end, but I'm hoping both will make it.

https://www.shroomery.org/forums/showflat.php/Number/25987049

I'm starting with an LC prepared from a single estero pan cyan spore syringe. There are pre-sterilized LC kits all over the web that make this super easy, just inject your syringe into the medium. In just a few days, it is ready to inject into your substrate/grain medium.

I have the following: dried horse manure, millet, vermiculite, straw, spring water, 50 pack of the 0.2 micron spawn bags (specifically Unicorn 3T 8"X5"X19"), and two of the 3 quart pyrex trays.

Asura said:
Quote:

This recipe makes about 7 quarts of substrate and is enough to fill two trays. I like 3 quart Pyrex trays. I will fill each tray almost to the top, leaving a little room for casing and I don't care having a little sub left over.

Horse manure 675g
Milo (rye or millet) 250g - not prepped in any way
Vermiculite 100g
Straw 75g
Water 1600g




Extras: Filter Print Post Top
Offlinetree frog
eats bugs


Registered: 09/14/23
Posts: 442
Loc: lives in trees
Last seen: 1 hour, 37 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28534080 - 11/08/23 10:16 AM (2 months, 18 days ago)

I'm trying something similar with Bisporus.

Dropped wedges off agar straight into small mycobags day before last.  It's been really slow on millet and I haven't had much luck with LCs so far.


--------------------
Listen to the silence behind the engines' noise.  Jesus, Sweets, listen.  Hear it?  It's a love song.
For whom?
You are loved.
~ David Foster Wallace, Westward the Course of Empire Takes Its Way


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tree frog]
    #28535445 - 11/09/23 10:34 AM (2 months, 17 days ago)

tree frog said:
Quote:

I'm trying something similar with Bisporus.

Dropped wedges off agar straight into small mycobags day before last.  It's been really slow on millet and I haven't had much luck with LCs so far.


Thanks tree frog for update! I'm preparing the LC next week, have read around 8 days from spore syringe to fully grown out LC liquid, can be tested on agar plate if desired.


Extras: Filter Print Post Top
OfflineReishiman
Hobby grower
Male User Gallery


Registered: 08/03/23
Posts: 113
Loc: Neither here, nor there
Last seen: 2 hours, 57 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar] * 1
    #28544053 - 11/15/23 01:46 PM (2 months, 11 days ago)

Op. Didn't read everything yet but I'm also trying to grow pans and have been having problems similar to you I think except I don't have a flow hood. Are you saying that pans are more difficult to grow on pasteurized substrate? I was thinking that my problems must be due to not having cleaned the original culture enough because I grew cubes alongside and those grew fine. I believe I've also seen numerous examples of pans grown on pasteurized substrate on this site. Either way I might try a sterilized substrate and maybe slurry inoculate them once I have some good cultures.


--------------------
Animals are something invented by plants to move seeds around. ... Terrence McKenna

A psychedelic point of view is one that values consciousness... Terrence McKenna


Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,042
Trusted Cultivator
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: Reishiman] * 2
    #28544098 - 11/15/23 02:26 PM (2 months, 11 days ago)

Tregar thank you for T/A on page 1.

Prodigy, your sig always makes me grow a big rubbery one.

That is all i have to add.

:threadmonitor:


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Extras: Filter Print Post Top
OfflinemanOwar
Siphonophore


Registered: 07/15/17
Posts: 315
Last seen: 22 hours, 33 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28560861 - 11/29/23 02:15 PM (1 month, 28 days ago)

Quote:

tregar said:. I I'm no longer following the Gordotek method, learned the hard way on 1st grow it's not for me, too unsterile.





You keep missing an important point, of some of the most helpful comments. Your conclusion is the opposite of the message that many other users have been trying to get across to you.
The source of your contamination is almost certainly because you sterilized your bulk substrate, and casing material, killing off the beneficial microbes, that provide protection from contamination. You seem to have hyper fixated on mycobags and decided that not using mycobags, was your mistake. But ignored the advice of much more experienced cultivators, none of whom mentioned mycobags as a solution to your problems. They have repeatedly mentioned that you don’t need to use your flow hood when inoculating your bulk substrate, or when adding your casing layer, because those materials should be pasteurized, rather than sterilized. If you do grow in mycobags, do not break the substrate up and dumpt into cake pans, that is a great way to kill the mycelium. Either use cake pans or mycobags, not both.

The main point here is that the TEK you used is not “too unsterile,” rather that it includes sterilization in unnecessary, counterproductive, or even harmful ways and sterile techniques in places where they are superfluous.

So if anything, the tek you used, is actually too sterile.


Edited by manOwar (11/29/23 02:22 PM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: manOwar]
    #28564663 - 12/02/23 02:02 AM (1 month, 25 days ago)

Reishiman said:
Quote:

Op. Didn't read everything yet but I'm also trying to grow pans and have been having problems similar to you I think except I don't have a flow hood. Are you saying that pans are more difficult to grow on pasteurized substrate? I was thinking that my problems must be due to not having cleaned the original culture enough because I grew cubes alongside and those grew fine. I believe I've also seen numerous examples of pans grown on pasteurized substrate on this site. Either way I might try a sterilized substrate and maybe slurry inoculate them once I have some good cultures.




elasticaltiger said:
Quote:

Tregar thank you for T/A on page 1.

Prodigy, your sig always makes me grow a big rubbery one.



I could not be happier with the new grow.

I decided not to go the LC or liquid culture route.

1 month ago, I used a disposable sterile scalpel to scrape spores from off a pan cyan estero print onto 5 different agar plates in front of flow hood, worked terrific, I don't recommend starting from a spore syringe, I tried and it was no where near as fast as just flicking spores off a print into the agar.

Another easy way to do this is as follows from waylitjim: https://mycotopia.net/topic/3295-waylits-pan-cyan-tek-plastic-jars/

Quote:

3- inoculate with spores, liquid culture, agar wedge or slurry
I like to shoot these containers with 6 cc of mycelium water. This way
the containers are completely colonized within 15 days and ready to fruit.

For mycelium water, shoot 6 ccs of sterile water onto an agar plate, mix up
the mycelium and water using the needle tip, and draw back into the syringe.
No need to dig into the agar layer, just wipe the myc. off the surface of the agar.
(Note, when starting with spores, try adding more BRF to the recipe.)



When each grain jar had lid opened, and an agar wedge inserted in front of flow hood, the growth took off in just a couple of days. In 15 days all 5 jars were completely grown out with mycelium, I shook the jars every 5 days and this really speeded things up.

I already had several 3 lb bags of pre-pasteurized mix of horse manure, vermic, water, coir, wheat straw which I cut open and emptied each 3lb bag into 1 mycobag in front of flow hood.

I then sterilized both 3 lb bags in the pc for 1 hour at 15 psi. I added 3 quarts of water to canner.

I let cool overnight, then put mycobag in front of flow hood, removed the clothespins from bag, opened up bag in front of flow hood, and poured one whole jar of mycelium grown out grain into each of 2 bags. This was sealed immediately after with a 25.00 impulse sealer inside the flow hood, worked like a charm.

Kept at a constant 77 degree F with the myco tent with lava rock heater, to my disbelief, the bag already showed signs of pure white growth all over the place in the bag 1 day later, and 3 days later I could not believe how great they looked, over-run with mycelium, at this rate 7 more days and they will be ready to be crumbled up and put into 4.5 quart pyrex trays for 1 week to recover, I'll place foil over the pyrex and poke a few holes for gas exchange. Then each pyrex tray will be ready for the casing and fruiting.



Edited by tregar (12/03/23 08:06 AM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28566357 - 12/03/23 07:35 AM (1 month, 24 days ago)

Reishiman, to answer your kind question, detail for above post:

Found for less than price of a discounted movie ticket: 3lb bags of pre-pasteurized manure base casing mix, these can be found all over the net from all different places in various sizes. These bags come pre-pasteurized for two plus hours. They have horse manure, coir, shredded wheat straw, minerals, hydrated lime, ph buffers like gypsum, and correct amount of spring water to bring to field capacity.

Had simply cut open the bag and poured it into one 8 x 5 x 19 large unicorn 3T 0.2 micron filter bag, then sterilized it for 1 hour at 15 psi, this way to sterilize not only the unicorn bag but it's contents, asura and blue helix and waylitjim all sterlized their substrates, I'm not taking any chances.

These are two better photos on day 4 after pouring 1 grain spawn jar into each substrate bag, the original fan broke that was with tent, so the normal all day temp dropped from 77 to 74, replacing with a better pc fan on the way here soon. The fan acts like a convection oven, blowing warm air from the lava rock heater below upwards to all the martha tent. With a good fan, the tent maintains a constant 77 degree F all over tent when set with electronic thermostat.

The reason you still see clothespins on the bag is because after I impulse sealed the bags in front of flow hood using a twenty five dollar impulse sealer with 4,000 good reviews, I put the clips back on just to keep the top of bag upright (front falling over) as it lays against the side of martha tent.

Asura mentioned here https://www.shroomery.org/forums/showflat.php/Number/25987049 to allow 10 days for the bags to grow out completely, and to break up the bag and gently mix things up again on day 5 which will be tomorrow for me to do.



Asura:
Quote:

Colonization

I let the bags colonize for ~10 days (at about 72°F) in a dark closet. I don't know if it's the lack of light, the still air in the closet or a combination of both, but my bags grow out better this way. Prior to doing this, I used to just keep the bags on a rack in the kitchen. I would see a lot of metabolite expression. Since moving to the closet, my growth is stronger and the bags are mostly metabolite free.

12 hours or so after inoculation, I gently mix up the substrate for the first time. At day 5, I will also break up the bag and gently mix things up again. By day 10, the bags should be ready to be laid to trays. If it is not,I will toss it.




Edited by tregar (12/03/23 11:24 AM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28588038 - 12/18/23 08:04 AM (1 month, 9 days ago)

18 days later the 3 bags are completely colonized, they were shaken once every 7 days, will allow around a week for it to continue to sit, blue helix recommended letting it sit a week (after it's colonized) before going to next stage...

...where the bags are cut open, broken up, and laid in a pyrex tray around 2" deep or so with foil put on top, and several toothpick holes poked to allow for air exchange, this next stage will be the reconsolidation stage for 5 days before the mm thick casing soil is put on top.

I noticed that when it was below 50 degrees outside, the temp in the tent would not go above 72 degrees at night or early morning, so modified the original heater by removing middle board, scooting it to the end, installing a second 150 watt ceramic reptile heater lamp in parallel wiring with the 1st heat lamp, installed a larger baking pan on top, filled with lava rocks....this took around 1/2 hour for the modifications using a drill and screw driver and cutter for the wires.

....and installed a variable speed pc fan to blow the warm air upwards from the two ceramic heater lamps....works wonderfully! Now even though the mornings are below 40 degree F, and around 67 in the house at night, the temp still stays at a steady 77 degree F all day anywhere in the tent, morning, and night, the 2nd heat lamp made all the difference.  



Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28594899 - 12/23/23 07:04 AM (1 month, 4 days ago)

1st pic, after cutting open completely colonized bag in front of flow hood and crumbling and laying it out to a 4.8 quart pyrex tray, leveled with clean gloves pre-rinsed in 70% alcohol, covered with foil, poked some gas vent holes with tooth pic, and covered the holes with 0.22 micron 3m adhesive gas exchange tiny stickies. 

To show that these pan cyan mushrooms can even be easily fruited from 1 pint wide mouth mason jars...

I had 2.5 to 3 lbs of leftover substrate (pre-pasteurized manure/coir/wheat straw mix), so I added several scoops to each of ten 1 pint mason jars from *arget or *almart and also found a dozen wide mouth lids with injection port and micron filters for cheap on-line, scooped the substrate into each jar, screwed micron filter lid onto each, covered with foil and pc sterilized all ten jars in pressure cooker for 1 hour at 15psi with 3 quarts water added to canner, let jars cool overnight, opened canner...

...and in front of flow hood, poured around 1" to 1.5" worth of grown out mycelium grain into each 1 pint jar, put lid back on, shook and swirled the jars to mix the grain with the horse manure substrate...

....put into martha tent between (temp controller set to 77 and 78 degrees F), and to my amazement, after only 1 day and night in tent, each of the ten jars shows super fast mycelium white snow like growth all over the jar...will shake the jars after 5 days, allow to grow out another 5 days, then will fruit easily just by adding a mm layer of sterile casing to ear jar, saturate casing with spray water, and place into fruiting chamber along with the pyrex trays which will be cased and fruited at same time.

It's freezing outside and lower than 65 degrees F at night, yet the martha tent with dual 150 watt ceramic reptile heater lamps and variable speed pc fan that blows the warm air up like a convection oven easily keeps the temp between 77 and 79 degrees F, where I set it, no problem keeping it very warm all day and night.



Edited by tregar (12/23/23 07:17 AM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28596107 - 12/24/23 06:07 AM (1 month, 3 days ago)

Really impressed with the 1 pint wide mouth jars full of substrate (several 1/2 cup scoops), day 2 shows the ten jars almost 1/3rd grown out after adding only 1 to 1.25" inch of grown out pan cyan estero mycelium grown out grain, and shaking well. You can tell the mycelium really loves the pre-pasteurized mix of horse dung, coir, wheat straw, minerals, spring water mix that was sterilized for 1 hour at 15 psi in the 10 jars.

There is already puffy white snow like mycelium covering the surface of the inside of the jars. After 3 more days of growth, will shake the jars well, and allow to recuperate, consolidate and grow out another 5 days. Then will be ready to case with a few mm of sterile casing, saturate casing with spray water bottle, and fruit within the martha tent in no time.

The wide mouth filter disk lids with 0.2 micron filter are really useful.



Edited by tregar (12/24/23 06:26 AM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28599643 - 12/27/23 09:46 AM (1 month, 13 hours ago)

Day 5 since adding grains to the 10 pint jars full of substrate, turns out the jars will not need any shaking, nearly fully colonized...will allow 5 more days for 100% colonization, then will add a few mm sterile casing (that I sterilized today for 45 min at 15 psi, added 1 quart water to casing so that only a few drops of water fall out when fist of it made, will allow to cool overnight) to the 4.8 quart pyrex trays and jars a few days from now.

Pic 1: day 5, all of 10 jars nearly fully colonized
pic 2: side of one of the 4.8 quart pyrex dishes
pic 3: bottom of one of the 4.8 quart pyrex dishes



Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28600561 - 12/28/23 07:12 AM (30 days, 16 hours ago)

The next day, after the casing had cooled down in the pc overnight, poured around a 1/4" sterile casing onto top of trays in front of flow hood, used a fork that had been cleaned with 70% isopropyl alcohol to smooth out...then sprayed the casing layer down heavily with distilled water after it was in place, then (optional) put a foil cover over tray just for 24 hours as the mycelia needs to poke through the casing just a bit before pins will form and keeping the top on seems to speed that initial move up, probably because it keeps the casing layer warmer, and put back into martha tent with temp controller set to normal 77 to 78 degree F.

The 10 pint jars will be ready to case in around 4 more days. They sit at bottom of tent waiting their turn soon.



Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28602095 - 12/29/23 10:47 AM (29 days, 12 hours ago)

Asura said: https://www.shroomery.org/forums/showflat.php/Number/25987049
Quote:


Fruiting (Part 1)

Successful fruiting of pan cyans requires the understanding of these factors: humidity, evaporation, temperature, and fresh air exchange (FAE).

Temperature

Pan cyans like temps in the 76-80°F range. If I could lock in a temp perfectly, I would have it exactly 77°F at all times. A little fluctuation doesn't seem to hurt, but if temps drop too low, especially before the first flush, it can ruin the entire grow. Drops can even happen in the middle of summer in Texas. This is why I have a heater in my grow room year around.

FAE
This is the easy part. FAE is always on. Sometimes that air is mixed with fog and sometimes it's just air. But there is always some amount of air going into the FC.

Some growers handle FAE using a repeat cycle timer that simultaneously pulls in fresh air while exhausting the old air. I prefer the always on model. It's just one less thing to think about.

Humidity & Evaporation

Pan cyans thrive a high humidity environment. However, evaporation is a major pinning trigger (by all observations). And evaporation cannot occur efficiently if your FC is always near 100% relative humidity (RH).

With this in mind, it's best to think of fruiting in two stages: pre-pin and post-pin.

During pre-pin, it's best to cycle humidity. I will set my humidity controller to a range around 90-98%. This means the fog will kick on at 90% and will shut off when it hits 98%. While on, moisture is being added and humidity will increase.

While off, humidity drops and evaporation will increase. This on/off cycle takes some amount of time, which is why some cultivators use a repeat cycle timer in lieu of a controller. Both can work well.

I don't want the environment to be too wet or too dry, so I do regularly adjust the range on my controller based on what I am seeing. Having an empty tray in the FC can really help with this dialing in. If my tray is bone dry when the cycle hits 90%, I know I can increase the range to something like 92-98%. If my tray is too wet at the high end, I might adjust the range to something like 90-97% or 90-96%. I will make whatever adjustments necessary based on what I am seeing with the empty tray.

In my current grow, for instance, I have the range set to 92-97%. These adjustments were made over the
course of the first three days of fruiting.

Post-pin is easier to manage. Once, pinning has occurred the substrate will continue to put out flush after flush. I don't worry about the humidity cycling once the initial pinning has occurred. After the pins start coming in I usually set my controller to a tight range like 94-95% to keep the environment mostly humid. Even with the humidity on, some evaporation will still occur.

RogerRabbit said:
You can have 100% humidity right at the substrate surface, while having +/-95% humidity within the air of the fruiting chamber itself.
Even at 99% humidity, if you're getting good fresh air exchange, evaporation from the substrate will occur.



After 24 hours removed the foil cover from trays which had several toothpick sized air holes in it...filled humidifier with water, set humidity on humidity controller to TURN ON at 90 to 92 degrees humidity, and TURN OFF at 97 degrees humidity. Set variable PC fan blowing warm air up from the dual 150 watt ceramic lamp reptile lava rock heater to 2.5, low speed very quiet with still plenty of fresh air all thru the tent.

pic 1: trays look like this after removing the foil, substrate 100% colonized by the mycelium
pic 2: monsoon humidifier blows a rolling humidity over the trays (and jars when cased in a few days)
pic 3: humidity controller set to TURN ON at 90 to 92 degrees humidity, TURN OFF at 97 degrees humidity
pic 4: variable pc fan set to around 2.5 speed (low)
pic 5: monsoon humidifier set to around 3 setting (low)
pic 6: day 7 after adding grown out mycelium grain (1 to 1.25") to each of 10 jars with substrate and shaking, will case all jars in around 3 more days.



Edited by tregar (12/29/23 10:58 AM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28604227 - 12/31/23 07:02 AM (27 days, 16 hours ago)

Ran across a problem with the Chinese made humidity controller I was originally using, it had no calibration feature, so even though the sensor was several points off, I was not able to calibrate to the actual real humidity in the tent, as I have 3 different humidity meters, all very accurate, while the Chinese made monitor was way off...

...so found a much better INKBIRD humidity monitor controller with built in up and down CALIBRATION buttons, so was able to calibrate the new humidity controller to the actual real humidity based on the other meters in the tent, I was able to set the target high humidity to 97 and the "turn on differential" to 90 degrees humidity, so it was able to turn on humidity when it fell below 90 and turn it off when it reached 97. This new one worked incredibly well compared to the Chinese model.

....also place a large rim plastic cup way underneath the monsoon humidity hose on one of the racks, as every so often built up drips of water at the end of the monsoon hose would fall into the cup placed below the output tube, so the water would not just get dumped into the bottom of the tent as it fell...this keeps it much cleaner inside, so no drips of water would fall into bottom of tent or any of the mushroom trays.

pic 1: this inkbird humidity monitor controller works very well all day and night to keep humidity at a set TURN ON AT 90, TURN OFF AT 97, easy to calibrate too.

pic 2...it's also important to keep the monsoon humidifier with it's long expandable hose higher than the actual spot where the hose enters the tent, this way the built up water in the hose will exit the hose into cup far below in the tent as it drips out, otherwise the water can get built up in hose and will cause humidity to stop output. I used 2 x 5 gallon buckets on top a wooden stool to raise the monsoon humidifier high enough.

pic 3: the variable pc fan had to be turned back up to normal "8" mid-high setting in order to keep blowing the warm air up and thru all the tent, this made it easy to keep temp anywhere I set it, normally at 77 to 78 degrees F, the fan speed cannot be turned on low, or the temp will not go above 75 degrees when it is freezing outside and below 65 in house. The mid high "8" setting works very well, no matter how cold it is, it's able to keep a constant 77 to 79 degrees F in all the racks of the tent.



Edited by tregar (12/31/23 07:29 AM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28613744 - 01/08/24 07:18 AM (19 days, 15 hours ago)

Don't make the same mistake I did, so after 1.5 weeks, not a single pin, and the humidity controller became a major pain in the ass, as it's calibration would fluctuate and it drove me a little bit mad having to watch and tend to it often...

...so I wen't back to what Gordotek recommended, and just put the monsoon humidifier on a timer, 1/2 hour on, then 1/2 hour off, all day and night long...

...also rolled up around 2" of the tent from the bottom and secured it with clothespins, put the ceiling fan on LOW in the room, and cracked a window around the width of a clothespin, this made a major difference, also cracked the door to the grow room around 2" all the time, and now the inside of the martha tent is no longer like a 24 hour rainforest.:lol:

...The FAE or fresh air exchange is perfect now...for 1/2 hour the humidity climbs from 80 to a perfect 97 degrees fairly quickly when ON, maintains the 97, inches up to 98 during the last 5 minutes of the 30 min ON cycle, then drops down to an ending 80 during the last 5 min of the 30 min OFF cycle very slowly when OFF, now I'm finally seeing evaporation from the surface of the casing during OFF and good moisture of the casing when ON.

As I've read in a dozen other martha tent topics on this forum, all the variables of a tent can be tricky, and why so many only use monotubs, but these are pans and would require a slightly different monotub design from your normal cubensis (babayaga covers this, see post #1 link)..but this at least solved the problem for me. Hope to see pins within days or a week now.

The temp is still at a perfect 77 degrees all day and night, as the 77 temp still maintains easily even with the cracked window (40 degrees outside and 65 inside at night) and even with the rolled up portion of tent, this is due to the double lamp reptile heater.

An empty saucer next to the grow during the humidity cycling shows hardly any condensation when the timer is OFF and then it shows some moisture but not wetness like you want when ON.

For the first time I've seen tiny pools of water (that were forming during the day) at the bottom of the tent floor on the stand finally evaporate, now it's dry, I was lacking any kind of FAE or evaporation before, and evaporation is a pinning trigger.

New optimal Settings:
(1) variable speed pc fan at bottom of tent set to 2.5 (lowest setting, reduced from previous too high of 7)
(2) monsoon humidifier set to "2"
(3) ceiling fan in room set to "low"

In other words, just about everything set to "2" from the 2 inches of tent brought up, to the 2 on humidifier and 2 on pc fan.

Pic 1: rolled up the bottom of tent a tiny bit to allow FAE
Pic 2: cracked a nearby window slightly all the time
Pic 3: timer, push in every other button so that you get 1/2 hour ON and 1/2 hour OFF of the humidifier just like the gordotek grow.



Edited by tregar (01/08/24 10:24 AM)


Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,042
Trusted Cultivator
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28616318 - 01/10/24 12:01 PM (17 days, 11 hours ago)

Any fruits yet?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Extras: Filter Print Post Top
OfflineMwj12977
OLD DAD
 User Gallery
Registered: 12/12/23
Posts: 72
Last seen: 11 hours, 7 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: elasticaltiger]
    #28616707 - 01/10/24 06:10 PM (17 days, 5 hours ago)

Biggest issue I had with pans was the temperature difference between cubes. I was trying to colonize cubes and pans in the same space. Cube myc was taking off and the pans were not. Once I figured that out it was all good. It also colonized much slower than I was used to with cubes. I’m no expert and could be completely wrong here but raising the temps made them respond and gave me the desired results. As far as fruiting everything was in the same Martha and it all worked out well. Glad you posted this thread! I got some good info and insight from the community. I thank you for that and everyone for their thoughts.


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: Mwj12977]
    #28616733 - 01/10/24 06:36 PM (17 days, 4 hours ago)

elasticaltiger said:
Quote:

Any fruits yet?


Yes! finally today I came home and there are pins finally all over the trays!

mwj12977 said:
Quote:

Biggest issue I had with pans was the temperature difference between cubes. I was trying to colonize cubes and pans in the same space. Cube myc was taking off and the pans were not. Once I figured that out it was all good. It also colonized much slower than I was used to with cubes. I’m no expert and could be completely wrong here but raising the temps made them respond and gave me the desired results. As far as fruiting everything was in the same Martha and it all worked out well. Glad you posted this thread! I got some good info and insight from the community. I thank you for that and everyone for their thoughts.


Thanks mwj12977, the temp is at a constant 77 to 79 degrees in the tent even with the fan at the bottom of the tent on super low (2.5 lowest level) even though it's 40 degrees outside.

Yes, I'm super excited now, thanks for watching and tips elasticaltiger and mwj12977.

Got rid of the 24 hour rainforest conditions that got me no where for 1.5 week, did the following 2 days ago:

1. Cracking the window made all the difference for FAE, I lowered the crack in the window from 1" down to just 1/4" crack, and it allows in plenty of fresh air yet no noise from outside, and the crack is hidden well behind the blinds, yet loads of fresh air can now enter the room! I can even feel the wind coming in from the crack.

2. lowered the fresh air gap at the bottom of the tent from 2" down to just 1/2" opening, this allows around 3 plus or so fresh air exchanges per hour, which is great for pans, the 2" opening was overkill.

3. Installed one of those super bright LED strings from a reel and put it on a 12 hour time ON from 7am till 8pm.

I came home today after just 2 days of the above changes, and see pins all over the place, even on the sides of the pyrex, they all point upwards towards the light. I so happy to see pins as I've never seen a mushroom pin in all my life.

Super excited as I was loosing faith, this has been a long months long project with lots of learning curves.

Pic 1: Tent is rolled up 1/2" at bottom to allow FAE in, and CO2 to exit.
Pic 2: A reel of LED lights was suspended above the trays, super bright, no more having to open any curtains, as the light coming into room was just not enough anyways.
Pic 3:  A window near the martha tent is cracked 1/4" at all times to allow FAE into the room



Edited by tregar (01/10/24 06:42 PM)


Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,042
Trusted Cultivator
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28618881 - 01/12/24 04:09 PM (15 days, 7 hours ago)

How are the fruits coming along? Any pictures yet?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: elasticaltiger]
    #28628511 - 01/20/24 12:19 PM (7 days, 10 hours ago)

The tent for me at least has been a complete disaster, too many variable to contend with, nothing but aborts and no fruiting what so ever, side pins, even with a timer on a humidifier that goes up to 97% for 1/2 hour ON then down to 82% for 1/2 hour OFF has still not worked, and these are supposed to be optimum conditions for pan cyan. Variables that drive me crazy: how fast should fan run at bottom for FAE ?, how much of an opening on tent bottom ?, too moist ? too dry ? who knows..

I've switched to simplicity below, the tent can always be used to hold and grow grain jars, agar petri dishes, etc. at optimum temps.

Uses simply a sterile 54 quart (22.5 x 16 x 12.75") or a sterile 32 quart (18.5 x 14 7/8 x 11 1/8") tub

Sterilite 54 or 32 Quart Clear Gasket Box with Blue Latches & Gasket

--> Bod's Unmodified Monotub TEK:
https://www.shroomery.org/forums/showflat.php/Number/22337800/fpart/1/vc/1

Bod said "This tub fruiting method is extremely low maintenance. No fanning is required, mist if needed, which will be infrequently. The lid is NOT latched down, EVER."

--> Baba Yaga Panaeolus in monotubs:
https://www.shroomery.org/forums/showflat.php/Number/27637997/vc/1

Baba Yaga said "Member kirkeng convinced me with his well performing unmodified tubs to give unmodded a try and I have to say that these are my new favorites. With those I fruit right at spawn and run subs without a casing. The lid goes on upside down."

Just grow pan cyans in unmodified sterlite 54 or 32 quart tubs, the lid does on upside down.


Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,042
Trusted Cultivator
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28628907 - 01/20/24 05:25 PM (7 days, 5 hours ago)

Tregar, you went into this with so much confidence. What was your experience with growing mushrooms before? I wish you had reached out to the community for help with the issues you were encountering.

How did you select your cultures for the grow? Is there anything salvageable? It sounds like you just needed help dialing in the martha. The TCs tend to really try and dissuade new growers from automated setups for the reasons you've mentioned. They can be notoriously difficult to dial in.

I hope the tubs work out for you.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: elasticaltiger]
    #28630747 - 01/22/24 08:00 AM (5 days, 15 hours ago)

Elasticaltiger said:
Quote:

Tregar, you went into this with so much confidence. What was your experience with growing mushrooms before? I wish you had reached out to the community for help with the issues you were encountering.

How did you select your cultures for the grow? Is there anything salvageable? It sounds like you just needed help dialing in the martha. The TCs tend to really try and dissuade new growers from automated setups for the reasons you've mentioned. They can be notoriously difficult to dial in.

I hope the tubs work out for you.



Elasticaltiger...I've always been a good chemist, but really suck at growing all things, it's always been like that, give me something to extract, no problem, but I can't grow a darn thing.

I need help. I turned off the fan at the bottom of the martha tent, and turned on the ceiling fan to MEDIUM setting in the room, and changed tent bottom opening from 1.5" to 2.5".

This might be helping? the humidity goes up to 97 quickly while on 1/2 humidifier ON then down to 90 when OFF, perhaps it was drying out too quickly before, as humidity was dropping to 75 or 80 before. Now at least, it stays at 90 while off even when cold outside, this is more like the humidity parameters Asura used.

I will see how this works.

The window is still cracked 1/4" to allow fresh air into room also (depending on temp outside).

I don't know if the unmodified tubs will work during the winter as it get's down to 60 degrees in room at night, at least the tent stays warm.

Fresh air easily enters the tent from the bottom part that is rolled up due to ceiling fan running on MED, it's more of a slower air exchange which is what I think I needed, as things were drying up too quickly before.

I'll open up another colonized mycobag, put into tent and case 1/2 of it with just a few mm casing, other 1/2 of it uncased to see how it goes.

The fully colonized jars received only a few mm of casing and have also not reponding with pins or fruits at all, I'm hoping this modification with FAE helps.

It really sucks that I had clean grains, spawn and 100% still clean and colonized substrate in not only dishes (which I have since moved into aluminum cake pans) but jars as well. I did the hard part fine, people say fruiting is supposed to be easy, but just the opposite for me, may give up for good, to see no results. Work long weeks, work out twice a week as life long bodybuilder, little free time, just loosing faith in all this down the drain work. I've drank cactus tea over 150 times over many years, if this doesn't work, gonna stick with my cactus, just thought it might be possible to have an alternative as my rare cactus only provides so many limited opportunities for tripping, which have all exceeded my expectations anyways. The zero nausea Ayahausca capsules always work great, so either way I can just go back to what I'm used to....just took on more that I can handle.


Edited by tregar (01/22/24 08:26 AM)


Extras: Filter Print Post Top
Offlinetregar
Stranger
 User Gallery

Registered: 08/30/04
Posts: 1,047
Last seen: 16 hours, 6 minutes
Re: Tregar's super easy pan cyan or panaeolus cyanescens or copelandia cyanescens grow log, crown jewel of mushrooms [Re: tregar]
    #28635839 - 01/26/24 04:28 PM (1 day, 6 hours ago)

This is my thread on 50 pan cyan trip reports and combo with 70mg THH or pure tetrahydroharmine:
https://www.shroomery.org/forums/showflat.php/Number/28108398/fpart/1/vc/1

Sterilite 54 or 32 Quart Clear Gasket Box with Blue Latches & Gasket

--> Bod's Unmodified Monotub TEK:
https://www.shroomery.org/forums/showflat.php/Number/22337800/fpart/1/vc/1

Bod said "This tub fruiting method is extremely low maintenance. No fanning is required, mist if needed, which will be infrequently. The lid is NOT latched down, EVER."

--> Baba Yaga Panaeolus in monotubs:
https://www.shroomery.org/forums/showflat.php/Number/27637997/vc/1

Baba Yaga said "Member kirkeng convinced me with his well performing unmodified tubs to give unmodded a try and I have to say that these are my new favorites. With those I fruit right at spawn and run subs without a casing. The lid goes on upside down."
------------------------------------------------------------
Great news!

I ditched the martha tent (only use it to grow out grains, jars, or mycobags, love both). As I had to throw out the cake pan from the attempted fruit in martha tent due to it just not fruiting, lack of pins. Too many variables to adjust and trial and error for a martha at least for this beginner.

And put the estero pan cyan substrate cakes which I birthed and 2 new estero pan cyan completely colonized substrate from the mycobags into 2 cake pans, this is what they look like only 3 days later, almost reconsolidated back together as pure white snow all over the top.

And bought 2 of the 54 quart sterilite clear gasket tubs, put the birthed cakes into it, along with the 2 cake pans.

I put programmable seedling heat mats under the tub but separated by can lids, so not touching, set thermostat to keep it at 80 degrees below, and the tubs stay at 76 degrees even when it's 55 degrees outside and around 70 degrees F inside the house at night. The air outside is humid due to lots of rain outside recently.

Punched holes in a piece of box cardboard to run the LED string light that comes on a reel, and plugged it into the timer that turns it on from 7am till 7pm.

The lid is of course put on upside down as per Bod's tek in first link below, ceiling fan off but I do keep the window cracked 1/4" behind the blinds which lets in a bit of fresh air from outside.

I misted only once the 1st day, and already see baby mushrooms all over the 7 jar cakes, the 2 cake pans should finish substrate reconsolidation after being moved from 100% colonized mycobag to the cake pans in about 3 more days, the mycelium is moving super fast to make it like snow all over the top.

Here is an estero pan cyan mushroom I picked from one of the cakes already.

I did not have any pins or mushrooms what so ever using expensive humidifiers and dual reptile heater at bottom with adjustable fan, while using the martha tent for almost over a month now...and all the sudden after moving into the Bod's tubs I have mushrooms in just 2 days!

The humidity stays between 94 to 99% all the time in the tub and the temp at 76 degrees F as I have electronic temp/humidity gauges in each tub.

I am so excited as the martha tent did not work for me, but the tubs rule at least in my case, so happy! I see baby mushrooms popping up all over the cakes just since yesterday!

Thank you Bod and Baba Yaga !!!!!!!!!!!!!

Pic 1: I'm growing in two of the 54 quart sterlite tubs, and it's working !!!
Pic 2: Inside of one of the two tubs, the 7 birthed cakes the very first day they were put into tub, no mushrooms 1st day, but by next day, I have baby mushrooms all over the cakes, will take another pic when full grown.
Pic 3: I have a Vivo Home seedling heat mat 10" x 20.75" under each tub with can lids as spacers, and they fit perfectly and keep it perfect temp inside even with it being 55 degrees outside.
Pic 4: This estero pan cyan mushroom was picked already.
Pic 5: Piece of cardboard with punched holes on each end from large box with LED strip light reel is the lighting fixture.
Pic 6: Timer to keep lights on for 12 hours
Pic 7: LED strip light reel for price of a movie ticket.
Pic 8: Programmable seedling heat mat set to 80 degrees F underneath jar lids keeps it 76 degrees inside tubs during this January winter, even when it dips down to 50 degrees outside and 68 degrees in the house at night. Temp sensor probe for mats is stuffed underneath the heat mat itself as seen in 1st and 5th photos.
Pic 9: My pan cyan substrate broken up and added from mycobag seen fully reconsolidated in the cake pans or pyrex dish after 6 days.
Pic 10: Even adding a bit of grown out mycelium grain to jars full of sterilized horse poo/straw/vermiculite substrate become colonized in record time. I simply bought pre-packaged and pre-pasteurized 2.5 lbs bags of horse poo mixture substrate on line, added 2 cups or so to each pint jar, put lids on, sterilized at 15 psi for 1 hour, then in front of flow hood, poured some mycelium grain in, shook up, and it colonizes in a week.
Pic 11: The wide mouth jar cultivation lids with injection port and 0.2 micron filter can be bought on line for super cheap
Pic 12 and 13: The grain I started with, added wedges from Florida estero pan cyan agar plates to grain in front of flow hood. 

 


Edited by tregar (01/27/24 07:10 AM)


Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3  [ show all ]

Shop: Amanita Muscaria Store Amanita Extract   Kraken Kratom Kratom Capsules for Sale   PhytoExtractum Buy Bali Kratom Powder   Original Sensible Seeds Autoflowering Cannabis Seeds   Bridgetown Botanicals CBD Capsules   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   North Spore Boomr Bag


Similar ThreadsPosterViewsRepliesLast post
* Pan cyan's growkit first time need help avrilavigne 189 9 01/26/24 01:52 PM
by The Tao
* pan. cyans Hamurabi 3,950 14 09/26/04 04:00 PM
by george castanza
* Pan Cyan Question nimbus 2,712 11 04/25/04 02:57 PM
by HaveABeer420
* Re: -The difficulty of Pan Cyans?- Anonymous 4,025 2 12/12/99 11:37 AM
by Anonymous
* pan cyan & wood ? invi 4,171 10 09/14/03 05:30 PM
by Anonymous
* how much experience is needed to grow Pan Cyans? Psike 6,122 6 06/18/01 10:40 PM
by aluminum_can
* Pan Cyan Substrate? Zen Peddler 11,373 7 03/15/18 01:12 PM
by Mycolorado
* pan cyan and azure THEDANGLER 1,599 7 11/02/04 02:35 PM
by Hamurabi

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,222 topic views. 23 members, 243 guests and 64 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.048 seconds spending 0.01 seconds on 12 queries.