So you came to the shroomery asking for help but provided absolutely zero useful information on your post that could be answered in a simple sentence from one of us in the “Quick questions, quick answers” thread? Let's try to help us help you by not wasting time asking you basic questions and having to claw the information out of you. All these questions may not be applicable but if you have the information it may be helpful for all involved. If you have a different answer to any of these feel free to put it where you think it fits. MOST IMPORTANTLY IF YOU ARE ASKING ABOUT WHAT SOMETHING LOOKS LIKE…ADD SOME PICTURES! NO ONE CAN GUESS WHAT IS HAPPENING WITHOUT THEM. MORE MOST IMPORTANTLY MAKE SURE YOU PICTURES ARE WELL LIT AND ABLE TO BE SEEN!
GENERAL QUESTIONS
What ACTIVE strain or ACTIVE variety are you growing?
What are you using to inoculate your substrate/grains/medium with? Multispore syringe (MSS), Liquid Culture (LC), Semi solid liquid culture (SSLC), Agar.
What are you inoculating into? BRF (Brown Rice Flour), Grains (WBS, Rye, Wheat, Millet, ETC), LC, SSLC, All In One bag (AIO bag).
What substrate for water retention are you using? Coconut coir (Coco coir), Vermiculite (Verm), Manure, AIO bag.
Are you using a still air box (SAB), fan filter unit (FFU), or a Laminar flow hood (LFH) when you inoculate your media or to perform actions that require sterility?
If you are using Syringes of any kind to inoculate, are you flaming the needle until it is red hot between jars?
JAR, GRAIN, OR OTHER PREP QUESTIONS
Are you using a steamer (Pot of boiling water with lid) or a Pressure cooker (PC)?
Are you soaking your grains before PCing? If yes for how long? If no are you doing no prep?
Are you venting your PC before allowing it to reach pressure? If yes, for how long are you doing so?
How long are you steaming or PCing your materials for? Materials can be PF tek jars, grain jars, agar plates, agar bottles, syringes, and etc.
What material are you using as a filter for your grain jars? Synthetic Filter Discs (SFDs), Tyvek, Multipore Tape, Polyfill, Syringe filter.
What is the ratio of spawn (grains) to sub (coir) you are dealing with?
Was your substrate at field capacity before mixing?
Are you using a dry layer of verm with your pf tek jars?
What ratio of verm and BRF are you using for your pf tek jars?
AGAR QUESTIONS
What Tek Are you following to create your agar or what ratio and what ingredients are you using to create the agar? Ratios such as 500ML water to 10G agar to 10G LME (light malt extract).
Are you using no pours or pours for your agar? No pours are poured into the plates and PC’ed. Pours are when you use a media bottle then pour your agar plates with the use of a FH, LFH, or SAB.
If you are taking transfers from your agar to another plate are you flaming the scalpel before each transfer?
LENGTH OF TIME QUESTIONS
How long has it been since you inoculated your substrate/media/agar?
How long has it been since you spawned your colonized grains and mixed it with substrate?
How long has it been since you first saw pins, hyphal knots, or etc?
How long has it been since your shook your grain jars and how long has it taken for them to recover and begin colonizing again?
TEMPERATURE QUESTIONS
What temperature is your environment with your set up? Are you using a heating pad underneath the tub (bad idea) or a space heater (good idea) in the same room?
What temperature and for how long are you drying your mushrooms?
HOW DOES MY (OBJECT) LOOK?
Have you taken well lit and clearly visible photos?
Have you tested your LC or SSLC on agar to ensure it’s not contaminated?
Truthfully I didn't put too much effort into this as I started adding questions I realized it could get quite long. I'm also too lazy to figure out how to best group questions within a process to make it more streamlined. Furthermore I'm not entirely sure if this will even be useful as it'll be buried pretty quickly I imagine. If you have any suggestions on questions/grouping that can be done feel free to add them. I'll make changes to it from time to time if even one persons calls me a dip shit just to spite you.
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Edited by Screwup (08/20/23 05:27 PM)
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