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Invisibleno-tone
Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
skip a step?
    #2843851 - 06/30/04 04:09 PM (13 years, 26 days ago)

A friend has some equador (from spores) colonizing some SDA plates. She has had very good luck in previous attempts taking wedges and making myc syringes with them to inoculate grain.

She wants her shrooms soon as the summer doesn't last long where she lives.

The question is this: The plates look free of contams, should she just make a wedge transfer to the grain (to save time and steps) or isolate a few more times and/or make myc syringes?

ANy input appreciated!


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Man thinks. God laughs. - Jewish Proverb


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OfflineQandA
wanderer

Registered: 05/19/04
Posts: 352
Last seen: 12 years, 11 months
Re: skip a step? [Re: no-tone]
    #2843865 - 06/30/04 04:12 PM (13 years, 26 days ago)

I've read of people doing that with no problems at all.


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I'm not crazy...crazy.


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Invisiblemuse_sick
ĤŌĿŶĞЋ0$Ŧ

Folding@home Statistics
Registered: 12/15/03
Posts: 9,399
Loc: Giu La Testa
Re: skip a step? [Re: no-tone]
    #2844014 - 06/30/04 05:10 PM (13 years, 26 days ago)

yeh it's not a problem to go from agar wedge to grain
in fact thats the method described in TMC


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InvisibleDieCommie
Registered: 12/11/03
Posts: 28,165
Re: skip a step? [Re: no-tone]
    #2844017 - 06/30/04 05:12 PM (13 years, 26 days ago)

Im confused...what step are you skipping? :confused:


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Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
Re: skip a step? [Re: muse_sick]
    #2844215 - 06/30/04 06:13 PM (13 years, 26 days ago)

I apologize for being unclear:

Its not a question of whether its ok to inoculate with the wedge. The question is whether she should keep isolating on progeny plates to obtain a contam free piece of mycelium. She wouldn't want to stick a contaminated wedge in a jar.


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Man thinks. God laughs. - Jewish Proverb


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Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
Re: skip a step? [Re: DieCommie]
    #2844224 - 06/30/04 06:16 PM (13 years, 26 days ago)

The steps she would be skipping would be the transfer of the first wedge to another plate and then again onto a 3rd plate (this is to guarantee the resultant mycelium not carry contams).

The other step that would be skipped is the making of the spore syringe.


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Man thinks. God laughs. - Jewish Proverb


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InvisibleDieCommie
Registered: 12/11/03
Posts: 28,165
Re: skip a step? [Re: no-tone]
    #2844252 - 06/30/04 06:27 PM (13 years, 26 days ago)

Oh...well on agar if it dosent look contam, i doubt it is....thats one of the beauties of agar, its easy to see contam.


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Invisibleno-tone
Enema Bag Jones

Registered: 10/17/00
Posts: 1,091
Loc: Warm, Moist and Dark
Re: skip a step? [Re: DieCommie]
    #2844269 - 06/30/04 06:31 PM (13 years, 26 days ago)

unless the contam is not on the media itself but on the mycelium.


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Man thinks. God laughs. - Jewish Proverb


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