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SirHuntington
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Domestication of a wild cube: grow log 2
#28403415 - 07/22/23 12:09 AM (6 months, 4 days ago) |
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I've just come back from about a 2 year break from growing and wanted to do something that had been on mind for quite some time, to domesticate one of the wild mushrooms that I had found myself when I first started this journey. My pervious experiences with growing from these wild prints had been pretty hit and miss with the potency and/or the yield. I've challenged myself with starting from scratch with the biggest wild cube I found which I lovingly called "Monster" and had printed about 5 years ago. It's cap was about 20cm in diameter and was pretty old by the time I picked it, the spore print was very patchy.
Initially these spores where just scraped onto agar, however after two weeks I saw nothing, not even bacteria; and decided to make some spore syringes which I had never done before.

I selected the "Monster" spore print along with a wild print from 2019 and a print from the second flush of my first ever grow. These were left to hydrate for 24 hours before having a drop put to agar and swirled around the plate. After a week I saw nothing again and got impatient and decided to make another 3 plates of each syringe. They were all a bacterial mess, however I think the bacteria kick started the spores at least for monster as I saw germination within a few days. The other two syringes didn't germinate at all, but I had taken enough transfers from the cleanest spots of the monster plates that I wasn't worried.
From these transfers I had about 15 different plates running and have slowly whittled them down to 8, due to slower or weaker growth, or scalloping.

These plates are currently on either t3 or t4 depending on their growth and I plan on testing them all. Two of these samples have already been sent to grain, B2 and G4.

And the myc is just starting to jump off the grain. I'll be updating this post as things progress.
If people have any comments on what I'm doing or want to chime in with how they rapidly iterate through cultures and clones to find the best performers I'd be very happy to hear them! Cheers guys
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SirHuntington
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Re: Domestication of a wild cube: grow log [Re: SirHuntington]
#28420811 - 08/04/23 06:07 PM (5 months, 21 days ago) |
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Short update:
Jars are colonizing slowly, I think the grains may have been a little dry which could be affecting the colonization time.

I got impatient and shook them, this is two days after the shake and they look like they are recovering well.
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B Traven
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Re: Domestication of a wild cube: grow log [Re: SirHuntington] 1
#28421024 - 08/04/23 08:38 PM (5 months, 21 days ago) |
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I'm mainly responding in order to get updates on this thread, as I am very interested in working with wild prints when I get a chance.
I've had the best luck with getting spores to germinate after swiping by taking my inoculation loop, flame-sterilizing it, and then quenching it in a jar of sterilized water before scraping the print. Another option would be trying to make extra wet and soft agar. I've gone the making-spore-syringes route too, but prefer streaking now when I can get it to work. I made a bunch of swabs earlier this year, but won't get around to trying to grow from them until the fall.
Pics look good. Lots of things can affect colonization speed, and I'd rather have slightly-dry grains than slightly-wet ones.
My best way forward when it comes to both production and culture work has been to develop a continous rotation for all stages of growth. From agar, to masters, to quart jars, to tubs, there's basically a set amount of each stage going at any given time. Cultures are always being moved through a series of agar transfers, and then going to grain when ready. Some cultures are getting started from spores, while others are coming from grain and getting put back on agar. Everything is labelled so I can track the different lines I have running, and know which generation I'm on. New lines get a chance to prove themselves, and if they don't pass muster, then their market share goes to already-proven veterans. I don't really take a single culture line and expand it a bunch. Instead, I treat each line like a sourdough starter, and take a little off the top of each quart that I like before spawning it. If a line has been pushed out to an excessive number of generations, but I really like it and don't have any backups on agar, then I'll just go grain to agar and start the cycle all over again.
I pretty much just work with plate pins and don't get too hung up on potency testing anymore, as all of my harvest is getting ground up and mixed together. More of a population biology approach to culture selection. There's lots of other ways to slice that banana, and that's just what I've settled into for now.
All of this helps me keep a decent number of lines in development without going nuts, and having a workflow that doesn't require a ton of thought. If I come across anything that I really like and want to preserve, then I can take tissue samples, prints and swabs for long-term storage. Otherwise, I just need to keep the ball rolling and maintain my labelling regime. I naturally develop opinions and attitudes about the different cultures I'm working with, and end up dropping some while giving more space to others.
-------------------- Beware of advice- even this.
Edited by B Traven (08/04/23 08:43 PM)
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SirHuntington
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Re: Domestication of a wild cube: grow log [Re: B Traven]
#28430369 - 08/11/23 09:26 PM (5 months, 14 days ago) |
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Both jar are almost at 100% colonization after a shake a few days ago. Will most likely spawn them some time this week just need to wait for the last of the uncolonized grain to get covered.

B Traven I'm in the process of getting to that stage where every few days I'll have some sort of mycology work to do whether it be making transfers, A2G, spawning to bulk, or harvesting/dehydrating. I'm making sure to label all of my cultures well so I know where I'm at and can keep a record of their performance as the generation progress.
I've already started to cull the cultures that had slow growth or plates that would continually scallop even after multiple transfers. I'm not too sure how I'm going to bio-assay all of these different cultures as I don't want to be tripping every weekend
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: Domestication of a wild cube: grow log [Re: SirHuntington]
#28430405 - 08/11/23 10:13 PM (5 months, 14 days ago) |
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A cool project. One that might go a lot of ways. I like the approach so far.
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LurkingLizard
Professional Noob


Registered: 08/03/23
Posts: 14
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Re: Domestication of a wild cube: grow log [Re: Pastywhyte]
#28430427 - 08/11/23 10:38 PM (5 months, 14 days ago) |
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Can't wait to see how this pans out  Wild prints are like a box of chocolates, you never know what you're gonna get
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Stipe-n Cap


Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: Domestication of a wild cube: grow log [Re: LurkingLizard]
#28430556 - 08/12/23 05:59 AM (5 months, 14 days ago) |
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med man
A equal opportunity offender

Registered: 03/13/23
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Re: Domestication of a wild cube: grow log [Re: Stipe-n Cap]
#28430770 - 08/12/23 10:21 AM (5 months, 14 days ago) |
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-------------------- Somewhere between chaos and disorder.
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SirHuntington
Stranger


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Re: Domestication of a wild cube: grow log [Re: med man] 1
#28474506 - 09/19/23 01:43 AM (4 months, 7 days ago) |
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Good to see a few people following along!
Sorry about the wait there hasn't been a lot to report until a few days ago. Both tubs are 100% colonized now with the B2 tub starting to pin. Pin set is pretty sparse at the moment, mostly around the sides of the tub. I attribute this to not being able to maintain proper surface conditions, every time I check I'm having to mist I think I'm going to up my coir to water ratio from 1:4 to 1:4.5 for the next set of boxes and see if that helps. I think I can see the first primordia beginning to form in the G4 tub as well.
Interestingly enough it appears that the B2 genetics like to blob which is a first for me. Do you think this is a genetic issue or could be caused by the environment as well? The jars were slightly bacterial but I've never had a culture behave this way before. There are a few normal looking pins scattered around so hopefully I'll be able to take a clone and a spore print from a decent looking one.
Cultures A and E jars almost 100% colonized. Will most likely get them into tubs this weekend.
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DERRAYLD
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Re: Domestication of a wild cube: grow log [Re: SirHuntington]
#28474515 - 09/19/23 01:59 AM (4 months, 7 days ago) |
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Nice work, I've been working with a wild Natalensis as well. Takes a lot of patience but it's worth it.
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Tiamo
Trust in LITFA



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Re: Domestication of a wild cube: grow log [Re: DERRAYLD]
#28474627 - 09/19/23 07:11 AM (4 months, 7 days ago) |
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--------------------
If you have used a Miraculix Psilocybin QTest, could you please share your results? Shipping free Ps. natalensis spore prints to any address in The Netherlands, just
Mush love
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