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Offlinenikica
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Registered: 08/03/23
Posts: 8
Last seen: 3 months, 20 days
What affects potency
    #28418817 - 08/03/23 02:03 PM (5 months, 23 days ago)

I acquired good genetics. Cloned from half-dry mushrooms that have been at the very top in terms of potency for years. Raised them. Dried in a dehydrator at 70 degrees celsius for about 12 hours. Sometimes more, sometimes less, so they were completely dry. Shared them around and ate them myself, and I see that's not it. They are stronger than all the ones I had before, but the visuals are missing and when I compare them with the original genetics, they are not up to par. After eating the same amount, mine are weaker. Why and how can I change it.
The man who has the original genetics doesn't want to say anything about the growing details. He doesn't even know I cloned his genetics.
There must be some catch. Is there any book, verified scientific research about what and to what extent affects potency? Because this is frustrating. His mushrooms are always white with bruises and are white and bluish inside. Almost like they are fresh. They look like they haven't been dried in a dehydrator. I don't know what it could be anymore. Do you guys have some advice for me. Im doing Philly golden teacher method with agar, rye, coco, verm and gypsum. Please help me


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InvisibleStipe-n CapMDiscord
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Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: What affects potency [Re: nikica] * 1
    #28418843 - 08/03/23 02:28 PM (5 months, 23 days ago)

Careful slection while culturing, potency is genetic.

There's no magical secret or ingredient, condition, etc. Potency varies from culture to culture, between species, and even variations in potency between mushrooms of the very same flush.

If you find x dose unsatisfactory, eat more. If that is unsatisfactory, start from spore to hunt the appropriate clone; if still unsatisfactory, grow a more potent species.

Don't expect much from cloning partially desiccated fruit. Just ask the guy/gal for a culture :shrug:



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InvisibleMicroMycelium
Curious Optimizer

Registered: 06/06/23
Posts: 461
Loc: Probably at work
Re: What affects potency [Re: Stipe-n Cap]
    #28418854 - 08/03/23 02:36 PM (5 months, 23 days ago)

:whathesaid:
While I feel like secretively ripping off someone’s work and hiding it while asking them details on their specific process is rather unethical…

Genetic differences, and different environmental factors like temperature/light/storage methods ect are among a variety of factors that can impact active contents of your fruits.

He had to dial that in so if you want to as well, put in the work. Grow, test, replicate, test, stabilize


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InvisibleStipe-n CapMDiscord
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Registered: 08/04/12
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Loc: Canada
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Re: What affects potency [Re: MicroMycelium] * 1
    #28418867 - 08/03/23 02:48 PM (5 months, 23 days ago)

Yeah, mushroom folks aren't very secretive about their methods, when we meet in person to talk shop or swap cultures I couldn't imagine someone being hesitant.


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OfflineShroomNugget
Mycologist Wannabe
I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 26 days, 5 hours
Re: What affects potency [Re: Stipe-n Cap]
    #28418897 - 08/03/23 03:10 PM (5 months, 23 days ago)

I'm trying to find something strong myself. Been cultivating about 6 months now, probably 30 shoeboxes and successful grows under my belt. I've grown: APE, SNAPE, AJMF, Albino Chodewave, TAM, Amazon's, P. Nats, and a few others. They're all pretty weak except the APEs. APEs were my first grow via PF TEK before I switched to shoeboxes.

I sent 10 different plates to 10 different tubs of a RedBoy Revert print I cleaned up. I was mostly testing for fruit and flush size and took the best culture of those. Come to find out the best flushes I kept weren't even that strong. Later I found out a different tub was considerably strong... Sigh! Wish I was patient enough to fully document and keep them all but I only kept the good flushers.

I'm finishing up a.ghost tub now that looks good, from a spore 1x Xfer, and hoping for the best. I'm  about to spawn two tubs of that RBR culture tonight, hoping to print a nice fruit and restart the process from germination until I get something potent. Is that how to go about isolating for potency?

What is the best and quickest method for finding a potent culture?


--------------------


Edited by ShroomNugget (08/03/23 03:12 PM)


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InvisibleStipe-n CapMDiscord
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Registered: 08/04/12
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Re: What affects potency [Re: ShroomNugget] * 1
    #28418900 - 08/03/23 03:12 PM (5 months, 23 days ago)

There is no quick way. Select clone, test clone, repeat. This takes time.

If you want fast + strong, grow cambos.


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OfflineShroomNugget
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I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 26 days, 5 hours
Re: What affects potency [Re: Stipe-n Cap]
    #28418980 - 08/03/23 04:10 PM (5 months, 23 days ago)

Not necessarily looking for the fastest way, more like a little step-by-step walk through.

For example: growing from spore produces fruits of a variety of potencies. How the heck do I know which shroom to clone if I can't test for potency, I.E. eat it? I feel like it's the chicken or eggs scenario, lol.


--------------------


Edited by ShroomNugget (08/03/23 04:11 PM)


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InvisibleMicroMycelium
Curious Optimizer

Registered: 06/06/23
Posts: 461
Loc: Probably at work
Re: What affects potency [Re: ShroomNugget]
    #28418988 - 08/03/23 04:17 PM (5 months, 23 days ago)

Quote:

ShroomNugget said:
Not necessarily looking for the fastest way, more like a little step-by-step walk through.

For example: growing from spore produces fruits of a variety of potencies. How the heck do I know which shroom to clone if I can't test for potency, I.E. eat it? I feel like it's the chicken or eggs scenario, lol.



A. obtain commercial lab equipment, take a sample, extract and measure actives, use the sample from the highest concentration to grow.
B. Eat it and use the one that feels the best.

The initial material used to inoculate determines its genetics regardless of form factor. Spores, LC, clone ect

Or are you asking how to perform a genetic analysis of spore cells to predict future prevalence of its compounds through germination? That’s not the most realistic approach IMO…
But I’m a noob so


Edited by MicroMycelium (08/03/23 04:34 PM)


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OfflinePandaskis
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Registered: 03/14/23
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Re: What affects potency [Re: MicroMycelium] * 1
    #28419007 - 08/03/23 04:23 PM (5 months, 23 days ago)

If youre looking for potency PE Varieties and Yetis are up there, i know thats not exactly your question :P, but if youre just looking for potency, those are some good ones. Theyre slow af though


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OfflineShroomNugget
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I'm a teapot User Gallery


Registered: 02/10/23
Posts: 355
Last seen: 26 days, 5 hours
Re: What affects potency [Re: MicroMycelium]
    #28419031 - 08/03/23 04:48 PM (5 months, 22 days ago)

Quote:

MicroMycelium said:
Quote:

ShroomNugget said:
Not necessarily looking for the fastest way, more like a little step-by-step walk through.

For example: growing from spore produces fruits of a variety of potencies. How the heck do I know which shroom to clone if I can't test for potency, I.E. eat it? I feel like it's the chicken or eggs scenario, lol.



A. obtain commercial lab equipment, take a sample, extract and measure actives, use the sample from the highest concentration to grow.
B. Eat it and use the one that feels the best.

The initial material used to inoculate determines its genetics regardless of form factor. Spores, LC, clone ect

Or are you asking how to perform a genetic analysis of spore cells to predict future prevalence of its compounds through germination? That’s not the most realistic approach IMO…
But I’m a noob so




But how do I clone a shroom that I ate and now know is potent? Its now in muh brelly, lol. That's my issue.


--------------------


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InvisibleStipe-n CapMDiscord
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Registered: 08/04/12
Posts: 7,623
Loc: Canada
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Re: What affects potency [Re: ShroomNugget]
    #28419050 - 08/03/23 05:03 PM (5 months, 22 days ago)

Clone the fruit, then dry it, make sure to appropriately label the culture. Eat the cloned fruit, or at least some of it, then determine if the plated clone is worth growing.

I have a clone selection criteria in my psuedo-casing thread in sig.


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Offlinemed man
A equal opportunity offender

Registered: 03/13/23
Posts: 185
Loc: The 3rd coast
Last seen: 5 days, 10 hours
Re: What affects potency [Re: ShroomNugget]
    #28419052 - 08/03/23 05:05 PM (5 months, 22 days ago)

Quote:

ShroomNugget said:
Quote:

MicroMycelium said:
Quote:

ShroomNugget said:
Not necessarily looking for the fastest way, more like a little step-by-step walk through.

For example: growing from spore produces fruits of a variety of potencies. How the heck do I know which shroom to clone if I can't test for potency, I.E. eat it? I feel like it's the chicken or eggs scenario, lol.



A. obtain commercial lab equipment, take a sample, extract and measure actives, use the sample from the highest concentration to grow.
B. Eat it and use the one that feels the best.

The initial material used to inoculate determines its genetics regardless of form factor. Spores, LC, clone ect

Or are you asking how to perform a genetic analysis of spore cells to predict future prevalence of its compounds through germination? That’s not the most realistic approach IMO…
But I’m a noob so




But how do I clone a shroom that I ate and now know is potent? Its now in muh brelly, lol. That's my issue.





That's the challenge chasing a memory’s? It's all transitory.


--------------------
Somewhere between chaos and disorder.


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InvisibleAlmaDelita
Felines Forever
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Registered: 07/26/15
Posts: 439
Loc: United States
Re: What affects potency [Re: med man]
    #28419118 - 08/03/23 05:45 PM (5 months, 22 days ago)

It depends. Honestly, learn and practice. Are you sticking strictly to Ps. Cubensis? Try a different species. Eat on an empty stomach.
You're on your own, just careful man, don't jump in head first with a huge
dose, because you didn't feel anything the first times. Goodluck, be safe.


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Offlinenikica
Stranger
Registered: 08/03/23
Posts: 8
Last seen: 3 months, 20 days
Re: What affects potency [Re: AlmaDelita]
    #28419945 - 08/04/23 01:51 AM (5 months, 22 days ago)

Guys it seems you dont understand. Man has the same genetics over years. Their potency is always the same. I Took a clone from them- and potency is weaker. They look same, but is weaker. So unless he is spraying them with something or giving nutrients, what other factors can affect potency? Becasue there is still large gap between them.


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OfflinexShroomerx
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Registered: 08/15/11
Posts: 133
Last seen: 3 months, 15 days
Re: What affects potency [Re: nikica]
    #28419970 - 08/04/23 02:51 AM (5 months, 22 days ago)

It's like the secret ingredient in Grandma's cookies (Love), you left out some part of the "Love" he's giving them.  Maybe light (or none), or huge depth in substrate / more water, different grain, etc. 
But yes, you are right, sounds like he's doing something you're not yet.  To find out what might help, you'll have to setup 3-4+ different trays and make some variable changes to see what works better for you. 
Or, you could ask him how he grows, if he knows you know he grows, then he might be willing to share some tips with you.


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Invisibledna24
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Registered: 04/19/22
Posts: 360
Loc: Savages
Re: What affects potency [Re: nikica]
    #28420376 - 08/04/23 11:19 AM (5 months, 22 days ago)

technique and knowledge of the strain, seems since they have had it for years and it produces amazing stuff he has his process dialed in for that strain, you have to crack that code, its not like its a secret that is proven to work for every variety. could just be bad luck on the one you happened to clone from


--------------------
:flipthebird::stonedjerk: FUCK STIPE :stonedjerk::flipthebird:


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Offlinenikica
Stranger
Registered: 08/03/23
Posts: 8
Last seen: 3 months, 20 days
Re: What affects potency [Re: xShroomerx]
    #28421433 - 08/05/23 06:09 AM (5 months, 21 days ago)

All this things you mentioned shouldnt do much of a difference. Genetics is most important. Or am i wrong.


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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: What affects potency [Re: nikica]
    #28421446 - 08/05/23 06:22 AM (5 months, 21 days ago)

Could be a storage issue. Light degrades potency. Freezing can also degrade the actives. Also another factor is contamination, I’ve long suspected that bacterial contamination can override certain expressions, certainly pigment can be one. I suspect potency can be another.


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Offlinetrippleblack
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Registered: 12/01/19
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Last seen: 18 hours, 59 minutes
Re: What affects potency [Re: Pastywhyte]
    #28421486 - 08/05/23 07:22 AM (5 months, 21 days ago)

maybe you germinated some spore material when you tried to clone.. it could be any number of things.


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Offlinedrjohn2012
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Registered: 07/31/22
Posts: 63
Last seen: 2 months, 13 days
Re: What affects potency [Re: Pastywhyte]
    #28421491 - 08/05/23 07:25 AM (5 months, 21 days ago)

To me it is very tricky unless your have the equipment to actually measure the amount of Psilocybin.  At least for me the effect is so different each time even from the same flush.  Mood, diet of the day, other meds you may have in you.


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