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OfflinePortobelloPapi
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1st Time Growing in Unicorn Bags: Contamination?
    #28398489 - 07/17/23 08:59 AM (6 months, 9 days ago)

Just started cranking out some bags of golden oysters. The bags are 5# each of master mix that I PC’d for 3 hours between 15-17psi. The bags came out of the PC with moisture on the inside and were opened in front of a flow hood with as sterile technique as possible. After 2 weeks of colonizing they now look like this:


They exuded a lot of metabolites on plates but not much on the grain spawn. However, in the bags the metabolites really ramped up and now it looks like there are bacterial splotches. I’m just seeking confirmation on what these might be. My money is either mycogne or bacteria. Leaning toward bacteria because there are no fruitbodies yet.


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OfflineStromriderM
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: PortobelloPapi]
    #28399213 - 07/17/23 08:05 PM (6 months, 8 days ago)

Work on your sterile technique. They shouldn't be making metabolites on the plate. You have a bacteria issue


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OfflinePortobelloPapi
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: Stromrider]
    #28403573 - 07/22/23 07:00 AM (6 months, 4 days ago)

Update: ignored the advice saying I had bacteria and decided to fruit the blocks anyway. Here is one of them after about 4 days in the FC @ 70F and RH = 85-95%.




The other blocks are coming along but this one blows them out of the water. It is possible there was contam on the blocks but they were 100% colonized and when I applied pressure to the “splotches”, they wept what looked like metabolites and revealed white mycelium underneath. I want to believe the splotches were merely blisters of metabolites.


Edited by PortobelloPapi (07/22/23 07:02 AM)


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OfflineStromriderM
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: PortobelloPapi]
    #28405066 - 07/23/23 09:05 AM (6 months, 3 days ago)

You can ignore it all you want but you still have bacteria. Lions mane will fruit in the presence of bacteria but you end up with lions mane that looks terrible and is soggy like you have


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InvisibleMycolorado
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: Stromrider]
    #28405381 - 07/23/23 04:18 PM (6 months, 3 days ago)

The crazy thing is, those are oysters. :puke:


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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: Mycolorado]
    #28405444 - 07/23/23 05:26 PM (6 months, 3 days ago)

Oh shit you're right. I got my threads mixed up :lol:


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InvisibleMycolorado
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: Stromrider]
    #28405487 - 07/23/23 06:08 PM (6 months, 2 days ago)

Figured but wouldn’t hold it against you considering.


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OfflinePortobelloPapi
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: PortobelloPapi]
    #28405768 - 07/23/23 10:25 PM (6 months, 2 days ago)

Update #2: we have takeoff



The goldens are coming in (I assume) nicely. The big cauliflower looking masses bloomed into these lovely bouquets. To address what other people in this thread have said: I’m still not convinced I had a bacteria issue, although, I do have another bag that stalled after shaking and won’t colonize the bottom of its bag and another bag that has significantly less metabolites than the ones in my initial post on this thread. No plates I made ever had colonies or any indication of bacteria aside from the metabolites from mycelium that suggested their presence but could be attributed to stress or other factors. Again, the spawn jars I used did not show any signs of contamination either.


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OfflinePortobelloPapi
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: Stromrider]
    #28405774 - 07/23/23 10:36 PM (6 months, 2 days ago)

My pops thought they were the lions mane too hahaha. I can see how it’d be easy to mix them up. I wasn’t able to find much info online about the cauliflower looking growth pattern of the primordia. My working theory is that because I didn’t fold the bags over the top of the block, it allowed enough FAE and evaporation to initiate pinning on the top of the block rather than the sides and caused it. I’m not convinced though.


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InvisibleMycolorado
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: PortobelloPapi]
    #28406044 - 07/24/23 07:58 AM (6 months, 2 days ago)

Looks about 1Mx better.  :thumbup:


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OfflinePortobelloPapi
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: Mycolorado]
    #28406154 - 07/24/23 09:24 AM (6 months, 2 days ago)

Thank you for the compliment and checking out this thread. My only gripe is that I have larger quantities of smaller fruits instead of the opposite. They didn’t grow out of the slits that I cut so my goal is to get the next crop to grow like that and hopefully give me the appearance that I want.


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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: PortobelloPapi]
    #28406479 - 07/24/23 01:29 PM (6 months, 2 days ago)

Larger quantities of smaller fruits is what you get with yellow oysters. Just what they do


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OfflinePortobelloPapi
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Re: 1st Time Growing in Unicorn Bags: Contamination? [Re: Stromrider]
    #28406502 - 07/24/23 01:43 PM (6 months, 2 days ago)

Huh, good to know. I appreciate the info. I’ll probably give these guys another day in the FC before harvesting. I think I can push them a little further before they’re past their prime


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