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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Ask Quick Questions, Get Quick Answers [Re: enscred1312] 6
#28403691 - 07/22/23 09:18 AM (6 months, 4 days ago) |
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A canopy ๐
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enscred1312
Stranger



Registered: 04/08/23
Posts: 156
Last seen: 3 months, 22 days
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Re: Ask Quick Questions, Get Quick Answers [Re: bodhisatta]
#28403694 - 07/22/23 09:20 AM (6 months, 4 days ago) |
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Well that's my dream lol And what about the happiness of the substrate before fruiting ? ๐
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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,152
Loc: Stables
Last seen: 16 minutes, 2 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho] 9
#28403707 - 07/22/23 09:36 AM (6 months, 4 days ago) |
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Quote:
SirPsycho said: Looks dry and bacterial
Quote:
SirPsycho said: It's struggling to recover
Leave my wife out of this
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Nigel Stephens


Registered: 02/21/20
Posts: 103
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
#28403735 - 07/22/23 10:23 AM (6 months, 4 days ago) |
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So I made some slants using 2% LME agar in 17mm centrifuge tubes. They sat in the PC (closed but depressurised) while they cooled in front of the hood then I got them out, screwed the lids tight and slanted the rack of tubes while still liquid.
Immediately they came alive and started what appeared to be boiling, the liquid would pop and shoot up the tube into the lid and in some cases with enough vigour it dislodged the tubes from the rack. Iโll add that this continued for ages, almost until the agar gelled. So weird
Has anyone seen this happen, how can it be prevented? I donโt want agar on the inside of the lid, obviously.
Thanks ๐
Edited by Nigel Stephens (07/22/23 10:26 AM)
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milkboy
Child



Registered: 05/01/21
Posts: 2,295
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Re: Ask Quick Questions, Get Quick Answers [Re: Nigel Stephens]
#28403741 - 07/22/23 10:33 AM (6 months, 4 days ago) |
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Probably just didnt wait long enough before you pulled em out the PC, hamloaf recommends waiting an hour and a half AFTER your PC has returned to atmospheric pressure
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,901
Loc: Rent free in your head
Last seen: 2 hours, 10 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] 1
#28403749 - 07/22/23 10:39 AM (6 months, 4 days ago) |
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Quote:
Smellyhobbit said:
Quote:
SirPsycho said: Looks dry and bacterial
Quote:
SirPsycho said: It's struggling to recover
Leave my wife out of this
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,901
Loc: Rent free in your head
Last seen: 2 hours, 10 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: enscred1312]
#28403750 - 07/22/23 10:39 AM (6 months, 4 days ago) |
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Quote:
enscred1312 said: Well that's my dream lol And what about the happiness of the substrate before fruiting ? ๐
Good recovery
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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    "Mist your balls and fan your asshole" - Pandaskis, 2023
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MicroMycelium
Curious Optimizer

Registered: 06/06/23
Posts: 461
Loc: Probably at work
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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
#28403982 - 07/22/23 02:22 PM (6 months, 4 days ago) |
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If you spawn a โtiredโ grain jar does it just take longer to colonize the substrate?
I decided to mix things up and give one of my jars an extra shake at 50%~ after the initial 30% and itโs taking extremely long to recover. Two weeks later, and this is what it looks like compared to two other jars, which were not available at the same time and have already been spawned to bulk. Initially, it was looking clean with no issues, but it is taking extremely long to re-colonize, which I have read people say that means the mycelium is โtiredโ (provided it isnโt fighting off contams).
Picture for reference:
Picture for reference
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Pandaskis
Eating Bamboo



Registered: 03/14/23
Posts: 1,869
Last seen: 1 month, 1 day
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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium] 2
#28404021 - 07/22/23 02:48 PM (6 months, 4 days ago) |
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Quote:
MicroMycelium said: If you spawn a โtiredโ grain jar does it just take longer to colonize the substrate?
I decided to mix things up and give one of my jars an extra shake at 50%~ after the initial 30% and itโs taking extremely long to recover. Two weeks later, and this is what it looks like compared to two other jars, which were not available at the same time and have already been spawned to bulk. Initially, it was looking clean with no issues, but it is taking extremely long to re-colonize, which I have read people say that means the mycelium is โtiredโ (provided it isnโt fighting off contams).
Picture for reference:
Picture for reference
Never heard that term. But slow or poor recovery generally means bacterial contamination. Sometimes bacteria that you dont initially see presents itself after a shake. Honestly the term "tired" mycelium feels like a term made up for people who dont want to accept that their spawn is bacterial haha, but i very well may be wrong. 
Spawning "Bacterial" spawn will take longer to colonize or just stall, no idea about "tired" spawn
Edited by Pandaskis (07/22/23 02:52 PM)
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MicroMycelium
Curious Optimizer

Registered: 06/06/23
Posts: 461
Loc: Probably at work
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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
#28404068 - 07/22/23 03:14 PM (6 months, 4 days ago) |
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Quote:
Pandaskis said:
Quote:
MicroMycelium said: If you spawn a โtiredโ grain jar does it just take longer to colonize the substrate?
I decided to mix things up and give one of my jars an extra shake at 50%~ after the initial 30% and itโs taking extremely long to recover. Two weeks later, and this is what it looks like compared to two other jars, which were not available at the same time and have already been spawned to bulk. Initially, it was looking clean with no issues, but it is taking extremely long to re-colonize, which I have read people say that means the mycelium is โtiredโ (provided it isnโt fighting off contams).
Picture for reference:
Picture for reference
Never heard that term. But slow or poor recovery generally means bacterial contamination. Sometimes bacteria that you dont initially see presents itself after a shake. Honestly the term "tired" mycelium feels like a term made up for people who dont want to accept that their spawn is bacterial haha, but i very well may be wrong. 
Spawning "Bacterial" spawn will take longer to colonize or just stall, no idea about "tired" spawn
The material I read used tired as a way to described mycelium that expended a large amount of energy recovering from stressors like being shaken. If it fully colonizes the jar Iโll assume itโs bacterial and just spawn it separate away from my good tubs then.
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hazyhorse
scoobin



Registered: 03/19/19
Posts: 3,820
Last seen: 9 hours, 2 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium] 2
#28404086 - 07/22/23 03:23 PM (6 months, 4 days ago) |
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i donโt think โtiredโ myc is much of a thing either. for cubes, your jars should be about 100% at two weeks barring super low temps. my vote is in bacteria too. an extra shake shouldnโt stress myc out to the point itโs gonna stall. jars can look great until a final shake, which usually is pretty telling as to whether you got anything riding along in there. definitely still spawn it, if for nothing else than as a learning experience, but ime jars that move that slow rarely perform well
-------------------- you're not the first to set foot here, just another =================================== i love glass petris & you can too!! posts i constantly refer back to new to mushroom cultivation?? read this!! ===================================
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Pandaskis
Eating Bamboo



Registered: 03/14/23
Posts: 1,869
Last seen: 1 month, 1 day
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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium] 1
#28404111 - 07/22/23 03:43 PM (6 months, 4 days ago) |
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Quote:
MicroMycelium said:
Quote:
Pandaskis said:
Quote:
MicroMycelium said: If you spawn a โtiredโ grain jar does it just take longer to colonize the substrate?
I decided to mix things up and give one of my jars an extra shake at 50%~ after the initial 30% and itโs taking extremely long to recover. Two weeks later, and this is what it looks like compared to two other jars, which were not available at the same time and have already been spawned to bulk. Initially, it was looking clean with no issues, but it is taking extremely long to re-colonize, which I have read people say that means the mycelium is โtiredโ (provided it isnโt fighting off contams).
Picture for reference:
Picture for reference
Never heard that term. But slow or poor recovery generally means bacterial contamination. Sometimes bacteria that you dont initially see presents itself after a shake. Honestly the term "tired" mycelium feels like a term made up for people who dont want to accept that their spawn is bacterial haha, but i very well may be wrong. 
Spawning "Bacterial" spawn will take longer to colonize or just stall, no idea about "tired" spawn
The material I read used tired as a way to described mycelium that expended a large amount of energy recovering from stressors like being shaken. If it fully colonizes the jar Iโll assume itโs bacterial and just spawn it separate away from my good tubs then.
If you have axenic spawn, i believe you can shake to your hearts content and itll never get tired.
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Bunnykinz
shroomer


Registered: 06/09/23
Posts: 46
Loc: Land of Oz
Last seen: 1 day, 9 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
#28404118 - 07/22/23 03:48 PM (6 months, 3 days ago) |
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Hi guys, letโs say I made an isolate, a nice choady Melmak, then went back to spore, would would the resultant cultures be more likely focused around the choady phenotype, or tapping back into a broad range of Melmak genetics? How about something more โstable like Melmak TP, would going back to spore be likely to give thicker Melmaks in general because the pheno is more stabilised?
Thanks
-------------------- โNature loves courage. You make the commitment and nature will respond to that commitment by removing impossible obstacles. Dream the impossible dream and the world will not grind you under, it will lift you up. This is the trick. This is what all these teachers and philosophers who really counted, who really touched the alchemical gold, this is what they understood. This is the shamanic dance in the waterfall. This is how magic is done. By hurling yourself into the abyss and discovering it's a feather bed.โ โ Terence McKenna
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Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
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Re: Ask Quick Questions, Get Quick Answers [Re: Bunnykinz] 1
#28404256 - 07/22/23 05:59 PM (6 months, 3 days ago) |
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By going back to spore you do have a higher chance at finding the pheno, but you will have to do some digging. This is how we stabilise.
Take spores, find the pheno, take spores from that, grow it out and repeat. Typically you'll go 8 generations or so before it's stable.
-------------------- Being pissed on does not make you a real man.
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Nonagon
Bacon frying, sparrows chirping


Registered: 09/01/22
Posts: 1,077
Loc: Hyperspace
Last seen: 1 day, 5 hours
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Re: Ask Quick Questions, Get Quick Answers [Re: Bunnykinz]
#28404264 - 07/22/23 06:05 PM (6 months, 3 days ago) |
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Quick and quite possibly dumb question - Are vacuum bags 100% airtight? Clearly they are airtight enough to retain their vacuumed formโฆ
But given that you can smell mushrooms through the bag, they must have some amount of permeability? Maybe this is by design and somehow helpful for food storage?
I just opened an ounce and it isnโt quite as crispy as it was. I would usually chalk that up to residual moisture leftover from drying, except it weighs almost a gram more than it used to. Which would lead me to believe that outside air/moisture has entered over a period of months.
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Edited by Nonagon (07/22/23 06:06 PM)
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Guerrilla
Bumbaclart


Registered: 01/30/21
Posts: 3,170
Loc: United Kingdom
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Re: Ask Quick Questions, Get Quick Answers [Re: Nonagon] 1
#28404274 - 07/22/23 06:16 PM (6 months, 3 days ago) |
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- you should not be able to smell anything through the bags, sounds improperly sealed. maybe your sealer is not performing how it should or you have a shitty quality vacuum roll.
- are you storing with desiccant packs?
-------------------- Being pissed on does not make you a real man.
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Wizard_Shames
Shames



Registered: 04/12/23
Posts: 516
Loc: Indonesia
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Re: Ask Quick Questions, Get Quick Answers [Re: Guerrilla]
#28404277 - 07/22/23 06:24 PM (6 months, 3 days ago) |
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Iโve seen on tiktok using the uncle Ben rice bag knock them up then spawn looks easy but Iโve been lurking here since 2009 n I never read about that
-------------------- It is never too late to be what you might have been Every saint has a past, and every sinner has a future You will face many defeats in life, but never let yourself be defeated shames.

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Smellyhobbit
Actual Retard



Registered: 04/01/22
Posts: 11,152
Loc: Stables
Last seen: 16 minutes, 2 seconds
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Re: Ask Quick Questions, Get Quick Answers [Re: Wizard_Shames] 2
#28404492 - 07/22/23 09:09 PM (6 months, 3 days ago) |
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Itโs largely regarded as the foolโs method for growing mushrooms. It can work, but thereโs so many better ways of doing it that thereโs no real reason to attempt it
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CocaineBuffet
Stranger



Registered: 08/29/19
Posts: 3,455
Last seen: 14 hours, 29 minutes
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Re: Ask Quick Questions, Get Quick Answers [Re: Wizard_Shames] 2
#28404519 - 07/22/23 09:35 PM (6 months, 3 days ago) |
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Quote:
Wizard_Shames said: Iโve seen on tiktok using the uncle Ben rice bag knock them up then spawn looks easy but Iโve been lurking here since 2009 n I never read about that
Please remove yourself from TikTok, Reddit, and any teks less than 3 years old on the Shroomery (in regards to mushroom cultivation).
You are in a safe place.
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Danetz
Stranger
Registered: 07/11/23
Posts: 14
Last seen: 4 months, 16 days
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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet]
#28404570 - 07/22/23 10:10 PM (6 months, 3 days ago) |
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Wow. Thirty pages! Iโll have to read them all when Iโm feeling ambitious. But right now I have some questions if I may.
I'm new to the art of growing mushrooms (it is an art, isnโt it?). And I'm focusing on a post by "EvilMushroom666" found here: https://www.shroomery.org/forums/showflat.php/Number/15223324#15223324
And I'm posting here to specifically ask for some of the experienced people to comment on some steps in the process and help me with my apprehensions. I.e. confirm the process points I'm goimg to list now. Thanks.
Under the headings "Spore Syringe" and "Using a Glove Box" it says to assemble the syringe and needle in the Glove Box (SAB) and the take it out, flame-sterilize it, bring it back into the SAB, inject a jar, bring the syringe out, flame-sterilize it, bring it back into the SAB, inject another jar, etc. etc. until the jars are done.
I'm concerned that all this in and out could increase the chances of contamination above what it would be to just move all jars into the SAB and process them there, wiping the needle with Iso between jars. Is this a bad idea? The recommendations of in, out, in, out, also increase the chance of poking something or yourself with the needle. Is this flame procedure necessary rather than wiping with ISO?
Also, it says to remove the jars from the PC and "assemble them in your work area", remove the foil, and wipe them, top and sides, with Iso. What are the risks of getting Iso in the jars via injection ports by wiping them with โa paper towel soaked with Iso"?
And finally, after saying to remove the foil from the jars and wipe them down with Iso, and then put them in the SAB, it says to spray 10% bleach into the SAB to sterilize the inside. But you're spraying bleach onto the tops of the jars with the injection ports! Is this the right way to do it?
Thanks!
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