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InvisiblebodhisattaMDiscordReddit
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Re: Ask Quick Questions, Get Quick Answers [Re: enscred1312] * 6
    #28403691 - 07/22/23 09:18 AM (6 months, 4 days ago)

A canopy ๐Ÿ˜‚


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Offlineenscred1312
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Re: Ask Quick Questions, Get Quick Answers [Re: bodhisatta]
    #28403694 - 07/22/23 09:20 AM (6 months, 4 days ago)

Well that's my dream lol
And what about the happiness of the substrate before fruiting ? ๐Ÿ˜…


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OfflineSmellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho] * 9
    #28403707 - 07/22/23 09:36 AM (6 months, 4 days ago)

Quote:

SirPsycho said:
Looks dry and bacterial




Quote:

SirPsycho said:
It's struggling to recover





Leave my wife out of this


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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InvisibleNigel Stephens
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit]
    #28403735 - 07/22/23 10:23 AM (6 months, 4 days ago)

So I made some slants using 2% LME agar in 17mm centrifuge tubes.
They sat in the PC (closed but depressurised) while they cooled in front of the hood then I got them out, screwed the lids tight and slanted the rack of tubes while still liquid.

Immediately they came alive and started what appeared to be boiling, the liquid would pop and shoot up the tube into the lid and in some cases with enough vigour it dislodged the tubes from the rack. Iโ€™ll add that this continued for ages, almost until the agar gelled. So weird

Has anyone seen this happen, how can it be prevented?
I donโ€™t want agar on the inside of the lid, obviously.

Thanks ๐Ÿ™


Edited by Nigel Stephens (07/22/23 10:26 AM)


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Invisiblemilkboy
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Re: Ask Quick Questions, Get Quick Answers [Re: Nigel Stephens]
    #28403741 - 07/22/23 10:33 AM (6 months, 4 days ago)

Probably just didnt wait long enough before you pulled em out the PC, hamloaf recommends waiting an hour and a half AFTER your PC has returned to atmospheric pressure


--------------------
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OfflineSirPsycho
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Re: Ask Quick Questions, Get Quick Answers [Re: Smellyhobbit] * 1
    #28403749 - 07/22/23 10:39 AM (6 months, 4 days ago)

Quote:

Smellyhobbit said:
Quote:

SirPsycho said:
Looks dry and bacterial




Quote:

SirPsycho said:
It's struggling to recover





Leave my wife out of this





--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineSirPsycho
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Re: Ask Quick Questions, Get Quick Answers [Re: enscred1312]
    #28403750 - 07/22/23 10:39 AM (6 months, 4 days ago)

Quote:

enscred1312 said:
Well that's my dream lol
And what about the happiness of the substrate before fruiting ? ๐Ÿ˜…



Good recovery :shrug:


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  ๐Ÿ…‘๐Ÿ…ž๐Ÿ…ฃ๐Ÿ…ฃ๐Ÿ…›๐Ÿ…” ๐Ÿ…–๐Ÿ…๐Ÿ…๐Ÿ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023


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InvisibleMicroMycelium
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Re: Ask Quick Questions, Get Quick Answers [Re: SirPsycho]
    #28403982 - 07/22/23 02:22 PM (6 months, 4 days ago)

If you spawn a โ€œtiredโ€ grain jar does it just take longer to colonize the substrate?

I decided to mix things up and give one of my jars an extra shake at 50%~ after the initial 30% and itโ€™s taking extremely long to recover. Two weeks later, and this is what it looks like compared to two other jars, which were not available at the same time and have already been spawned to bulk.
Initially, it was looking clean with no issues, but it is taking extremely long to re-colonize, which I have read people say that means the mycelium is โ€œtiredโ€ (provided it isnโ€™t fighting off contams).

Picture for reference:
Picture for reference


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OfflinePandaskis
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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium] * 2
    #28404021 - 07/22/23 02:48 PM (6 months, 4 days ago)

Quote:

MicroMycelium said:
If you spawn a โ€œtiredโ€ grain jar does it just take longer to colonize the substrate?

I decided to mix things up and give one of my jars an extra shake at 50%~ after the initial 30% and itโ€™s taking extremely long to recover. Two weeks later, and this is what it looks like compared to two other jars, which were not available at the same time and have already been spawned to bulk.
Initially, it was looking clean with no issues, but it is taking extremely long to re-colonize, which I have read people say that means the mycelium is โ€œtiredโ€ (provided it isnโ€™t fighting off contams).

Picture for reference:
Picture for reference




Never heard that term. But slow or poor recovery generally means bacterial contamination. Sometimes bacteria that you dont initially see presents itself after a shake.
Honestly the term "tired" mycelium feels like a term made up for people who dont want to accept that their spawn is bacterial haha, but i very well may be wrong. :takingnotes:

Spawning "Bacterial" spawn will take longer to colonize or just stall, no idea about "tired" spawn


Edited by Pandaskis (07/22/23 02:52 PM)


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InvisibleMicroMycelium
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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28404068 - 07/22/23 03:14 PM (6 months, 4 days ago)

Quote:

Pandaskis said:
Quote:

MicroMycelium said:
If you spawn a โ€œtiredโ€ grain jar does it just take longer to colonize the substrate?

I decided to mix things up and give one of my jars an extra shake at 50%~ after the initial 30% and itโ€™s taking extremely long to recover. Two weeks later, and this is what it looks like compared to two other jars, which were not available at the same time and have already been spawned to bulk.
Initially, it was looking clean with no issues, but it is taking extremely long to re-colonize, which I have read people say that means the mycelium is โ€œtiredโ€ (provided it isnโ€™t fighting off contams).

Picture for reference:
Picture for reference




Never heard that term. But slow or poor recovery generally means bacterial contamination. Sometimes bacteria that you dont initially see presents itself after a shake.
Honestly the term "tired" mycelium feels like a term made up for people who dont want to accept that their spawn is bacterial haha, but i very well may be wrong. :takingnotes:

Spawning "Bacterial" spawn will take longer to colonize or just stall, no idea about "tired" spawn



The material I read used tired as a way to described mycelium that expended a large amount of energy recovering from stressors like being shaken.
If it fully colonizes the jar Iโ€™ll assume itโ€™s bacterial and just spawn it separate away from my good tubs then.:salute:


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Offlinehazyhorse
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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium] * 2
    #28404086 - 07/22/23 03:23 PM (6 months, 4 days ago)

i donโ€™t think โ€œtiredโ€ myc is much of a thing either. for cubes, your jars should be about 100% at two weeks barring super low temps. my vote is in bacteria too. an extra shake shouldnโ€™t stress myc out to the point itโ€™s gonna stall. jars can look great until a final shake, which usually is pretty telling as to whether you got anything riding along in there. definitely still spawn it, if for nothing else than as a learning experience, but ime jars that move that slow rarely perform well


--------------------
you're not the first to set foot here, just another
===================================
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posts i constantly refer back to
new to mushroom cultivation?? read this!!
===================================

๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ


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OfflinePandaskis
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Re: Ask Quick Questions, Get Quick Answers [Re: MicroMycelium] * 1
    #28404111 - 07/22/23 03:43 PM (6 months, 4 days ago)

Quote:

MicroMycelium said:
Quote:

Pandaskis said:
Quote:

MicroMycelium said:
If you spawn a โ€œtiredโ€ grain jar does it just take longer to colonize the substrate?

I decided to mix things up and give one of my jars an extra shake at 50%~ after the initial 30% and itโ€™s taking extremely long to recover. Two weeks later, and this is what it looks like compared to two other jars, which were not available at the same time and have already been spawned to bulk.
Initially, it was looking clean with no issues, but it is taking extremely long to re-colonize, which I have read people say that means the mycelium is โ€œtiredโ€ (provided it isnโ€™t fighting off contams).

Picture for reference:
Picture for reference




Never heard that term. But slow or poor recovery generally means bacterial contamination. Sometimes bacteria that you dont initially see presents itself after a shake.
Honestly the term "tired" mycelium feels like a term made up for people who dont want to accept that their spawn is bacterial haha, but i very well may be wrong. :takingnotes:

Spawning "Bacterial" spawn will take longer to colonize or just stall, no idea about "tired" spawn



The material I read used tired as a way to described mycelium that expended a large amount of energy recovering from stressors like being shaken.
If it fully colonizes the jar Iโ€™ll assume itโ€™s bacterial and just spawn it separate away from my good tubs then.:salute:




If you have axenic spawn, i believe you can shake to your hearts content and itll never get tired.


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OfflineBunnykinz
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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28404118 - 07/22/23 03:48 PM (6 months, 3 days ago)

Hi guys, letโ€™s say I made an isolate, a nice choady Melmak, then went back to spore, would would the resultant cultures be more likely focused around the choady phenotype, or tapping back into a broad range of Melmak genetics?
How about something more โ€œstable like Melmak TP, would going back to spore be likely to give thicker Melmaks in general because the pheno is more stabilised?

Thanks


--------------------
โ€œNature loves courage. You make the commitment and nature will respond to that commitment by removing impossible obstacles. Dream the impossible dream and the world will not grind you under, it will lift you up. This is the trick. This is what all these teachers and philosophers who really counted, who really touched the alchemical gold, this is what they understood. This is the shamanic dance in the waterfall. This is how magic is done. By hurling yourself into the abyss and discovering it's a feather bed.โ€
โ€• Terence McKenna


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InvisibleGuerrilla
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Re: Ask Quick Questions, Get Quick Answers [Re: Bunnykinz] * 1
    #28404256 - 07/22/23 05:59 PM (6 months, 3 days ago)

By going back to spore you do have a higher chance at finding the pheno, but you will have to do some digging. This is how we stabilise.

Take spores, find the pheno, take spores from that, grow it out and repeat. Typically you'll go 8 generations or so before it's stable.


--------------------
Being pissed on does not make you a real man.


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OfflineNonagon
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Re: Ask Quick Questions, Get Quick Answers [Re: Bunnykinz]
    #28404264 - 07/22/23 06:05 PM (6 months, 3 days ago)

Quick and quite possibly dumb question - Are vacuum bags 100% airtight? Clearly they are airtight enough to retain their vacuumed formโ€ฆ

But given that you can smell mushrooms through the bag, they must have some amount of permeability? Maybe this is by design and somehow helpful for food storage?

I just opened an ounce and it isnโ€™t quite as crispy as it was. I would usually chalk that up to residual moisture leftover from drying, except it weighs almost a gram more than it used to. Which would lead me to believe that outside air/moisture has entered over a period of months. 


--------------------
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Edited by Nonagon (07/22/23 06:06 PM)


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InvisibleGuerrilla
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Re: Ask Quick Questions, Get Quick Answers [Re: Nonagon] * 1
    #28404274 - 07/22/23 06:16 PM (6 months, 3 days ago)

- you should not be able to smell anything through the bags, sounds improperly sealed. maybe your sealer is not performing how it should or you have a shitty quality vacuum roll.

- are you storing with desiccant packs?


--------------------
Being pissed on does not make you a real man.


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InvisibleWizard_Shames
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Re: Ask Quick Questions, Get Quick Answers [Re: Guerrilla]
    #28404277 - 07/22/23 06:24 PM (6 months, 3 days ago)

Iโ€™ve seen on tiktok using the uncle Ben rice bag knock them up then spawn looks easy but Iโ€™ve been lurking here since 2009 n I never read about that


--------------------
It is never too late to be what you might have been
Every saint has a past, and every sinner has a future You will face many defeats in life, but never let yourself be defeated shames. 


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OfflineSmellyhobbit
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Re: Ask Quick Questions, Get Quick Answers [Re: Wizard_Shames] * 2
    #28404492 - 07/22/23 09:09 PM (6 months, 3 days ago)

Itโ€™s largely regarded as the foolโ€™s method for growing mushrooms. It can work, but thereโ€™s so many better ways of doing it that thereโ€™s no real reason to attempt it


--------------------
A Love Letter to New Growers
A Guide for New Growers
Growth 2023 - A Year In Review

Grow more shrooms. Eat more ass. :mushroom:



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OfflineCocaineBuffet
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Re: Ask Quick Questions, Get Quick Answers [Re: Wizard_Shames] * 2
    #28404519 - 07/22/23 09:35 PM (6 months, 3 days ago)

Quote:

Wizard_Shames said:
Iโ€™ve seen on tiktok using the uncle Ben rice bag knock them up then spawn looks easy but Iโ€™ve been lurking here since 2009 n I never read about that




Please remove yourself from TikTok, Reddit, and any teks less than 3 years old on the Shroomery (in regards to mushroom cultivation).

You are in a safe place.


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OfflineDanetz
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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet]
    #28404570 - 07/22/23 10:10 PM (6 months, 3 days ago)

Wow.  Thirty pages!  Iโ€™ll have to read them all when Iโ€™m feeling ambitious.  But right now I have some questions if I may.

I'm new to the art of growing mushrooms (it is an art, isnโ€™t it?).  And I'm focusing on a post by "EvilMushroom666" found here: https://www.shroomery.org/forums/showflat.php/Number/15223324#15223324

And I'm posting here to specifically ask for some of the experienced people to comment on some steps in the process and help me with my apprehensions.  I.e. confirm the process points I'm goimg to list now.  Thanks.

Under the headings "Spore Syringe" and "Using a Glove Box" it says to assemble the syringe and needle in the Glove Box (SAB) and the take it out, flame-sterilize it, bring it back into the SAB, inject a jar, bring the syringe out, flame-sterilize it, bring it back into the SAB, inject another jar, etc. etc. until the jars are done.

I'm concerned that all this in and out could increase the chances of contamination above what it would be to just move all jars into the SAB and process them there, wiping the needle with Iso between jars.  Is this a bad idea?  The recommendations of in, out, in, out, also increase the chance of poking something or yourself with the needle.  Is this flame procedure necessary rather than wiping with ISO?

Also, it says to remove the jars from the PC and "assemble them in your work area", remove the foil, and wipe them, top and sides, with Iso.  What are the risks of getting Iso in the jars via injection ports by wiping them with โ€œa paper towel soaked with Iso"?

And finally, after saying to remove the foil from the jars and wipe them down with Iso, and then put them in the SAB, it says to spray 10% bleach into the SAB to sterilize the inside.  But you're spraying bleach onto the tops of the jars with the injection ports!  Is this the right way to do it?

Thanks!


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