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OfflineDr. Sporesly
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Registered: 05/20/20
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Last seen: 1 month, 18 days
Tomentose vs mold on multiple transfers JF, Yeti, APE?
    #28403664 - 07/22/23 08:54 AM (6 months, 4 days ago)

Hey all,

I've been toying with a few different varieties on agar and I'm seeing some interesting things occurring, these have all been inoculated from MSS, and I've been using MEA with a bit of charcoal powder. They're all at different stages of transfer right now, and at a high level:
  • Jack Frost - Transfer #3
  • Yeti - Transfer #2
  • APE - Transfer #1


The Jack Frost being the most transferred so far (spore to agar on 5.18), what I thought was originally tomentose growth, I'm now thinking is very enmeshed mold but would love some second opinions. Note: I have original plates left from 5.18 and they are all still white, no green or obvious contam from what I can tell. Pictures below.



The Yeti looks very structured in terms of growth, but seems very cottony as well, maybe tomentose?:


The APE, actually looks really strong and clean to me:



Any help or guidance on any of the plates would be super appreciated. Thank you so much!


Edited by Dr. Sporesly (07/22/23 08:57 AM)


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InvisiblebodhisattaMDiscordReddit
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Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: Dr. Sporesly] * 5
    #28403689 - 07/22/23 09:17 AM (6 months, 4 days ago)

Use half as much malt and see what the morphology looks like on that mix


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OfflineDr. Sporesly
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Registered: 05/20/20
Posts: 72
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Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: bodhisatta]
    #28403702 - 07/22/23 09:26 AM (6 months, 4 days ago)

Quote:

bodhisatta said:
Use half as much malt and see what the morphology looks like on that mix




Thanks Bod, appreciate it. Would your hypothesis be that the agar is too nutritious?


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InvisibleBaba Yaga
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Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
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Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: Dr. Sporesly] * 3
    #28403891 - 07/22/23 01:18 PM (6 months, 4 days ago)

I would also try leaving the charcoal out. Some people had similar weakish growth with it in the mix.


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OfflineDr. Sporesly
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Registered: 05/20/20
Posts: 72
Last seen: 1 month, 18 days
Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: Baba Yaga]
    #28403946 - 07/22/23 01:58 PM (6 months, 4 days ago)

Quote:

Baba Yaga said:
I would also try leaving the charcoal out. Some people had similar weakish growth with it in the mix.




Thanks very much, I'll leave it out on the next batch.

Also, this is the MEA mix I've been using because it was recommended from a few  on the discord: https://www.amazon.com/gp/product/B008W7XHSU/ref=ppx_yo_dt_b_search_asin_title?ie=UTF8&psc=1

I'm wondering if I should just make my own for the next cut?

Side note, think it's tomentose or maybe embedded mold?


Edited by Dr. Sporesly (07/22/23 02:05 PM)


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InvisibleBaba Yaga
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Posts: 3,955
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Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: Dr. Sporesly] * 2
    #28404230 - 07/22/23 05:35 PM (6 months, 3 days ago)

Better to do your own agar. 1.5g-2g agar and 1%-2% Malt extract on 100ml water. I have looked at this product and besides malt extract it contains peptone and dextrose, all unnecessary additions IMO. Most people here are doing the simple malt extract recipe, it's just easier if you want to compare yours to others and you can always expand on it later on.

I'd say if your sterile technique is OK then your plates are more likely looking like this because of your agar cause they are all sharing a somewhat same appearance but one can only tell after you have switched to a standard recipe.


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OfflineDr. Sporesly
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Registered: 05/20/20
Posts: 72
Last seen: 1 month, 18 days
Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: Baba Yaga]
    #28405041 - 07/23/23 08:50 AM (6 months, 3 days ago)

Quote:

Baba Yaga said:
Better to do your own agar. 1.5g-2g agar and 1%-2% Malt extract on 100ml water. I have looked at this product and besides malt extract it contains peptone and dextrose, all unnecessary additions IMO. Most people here are doing the simple malt extract recipe, it's just easier if you want to compare yours to others and you can always expand on it later on.

I'd say if your sterile technique is OK then your plates are more likely looking like this because of your agar cause they are all sharing a somewhat same appearance but one can only tell after you have switched to a standard recipe.




Thanks Baba, super appreciate it!

Would you recommend mixing it up a bit and doing a round of BRF agar or something other than MEA, or should I just do MEA?


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OfflineDr. Sporesly
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Registered: 05/20/20
Posts: 72
Last seen: 1 month, 18 days
Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: Dr. Sporesly]
    #28406295 - 07/24/23 10:45 AM (6 months, 2 days ago)

Update: Going to take a stab at Frank's Malt-Extract Yeast recipe and will be doing transfers later this week:
  • Malt-extract yeast: 10g ELME, 10g agar, 1g yeast


Will check back once my transfers are done


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InvisibleTheAtlanteanS
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Registered: 06/25/18
Posts: 1,000
Loc: Milkyway Galaxy, Laniakea Supe...
Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: Dr. Sporesly]
    #28406341 - 07/24/23 11:21 AM (6 months, 2 days ago)

Quote:

Dr. Sporesly said:
Update: Going to take a stab at Frank's Malt-Extract Yeast recipe and will be doing transfers later this week:
  • Malt-extract yeast: 10g ELME, 10g agar, 1g yeast


Will check back once my transfers are done




I would drop your recipe even lower.

Try a few different recipes.

General Recipe is 10g/10/1g

I like to go: LME 7G/Agar 10G/1G Yeast

But if you are looking for structures and sectors, I would reduce even further


--------------------

Meditation is passive learning. Tabernacling with the one infinite Creator is passive learning. It is the learning from listening to the voice one cannot hear which answers questions which one cannot ask. The process cannot be known and cannot come into consciousness in local sense, yet the learning is there. And the strength of spirit and will which comes from this learning is helpful.
.

:egyptian:www.templeofhermes.org:egyptian:


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OfflineDr. Sporesly
Papa Smurf

Registered: 05/20/20
Posts: 72
Last seen: 1 month, 18 days
Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: TheAtlantean]
    #28406434 - 07/24/23 12:54 PM (6 months, 2 days ago)

Quote:

TheAtlantean said:
Quote:

Dr. Sporesly said:
Update: Going to take a stab at Frank's Malt-Extract Yeast recipe and will be doing transfers later this week:
  • Malt-extract yeast: 10g ELME, 10g agar, 1g yeast


Will check back once my transfers are done




I would drop your recipe even lower.

Try a few different recipes.

General Recipe is 10g/10/1g

I like to go: LME 7G/Agar 10G/1G Yeast

But if you are looking for structures and sectors, I would reduce even further




Thanks I'll give that a try. Baba was saying "1.5g-2g agar and 1%-2% Malt extract on 100ml water"

So maybe: LME 6G / Agar 6G / 1G yeast?


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InvisibleTheAtlanteanS
Forever Grateful


Registered: 06/25/18
Posts: 1,000
Loc: Milkyway Galaxy, Laniakea Supe...
Re: Tomentose vs mold on multiple transfers JF, Yeti, APE? [Re: Dr. Sporesly]
    #28406441 - 07/24/23 01:00 PM (6 months, 2 days ago)

Quote:

Dr. Sporesly said:
Quote:

TheAtlantean said:
Quote:

Dr. Sporesly said:
Update: Going to take a stab at Frank's Malt-Extract Yeast recipe and will be doing transfers later this week:
  • Malt-extract yeast: 10g ELME, 10g agar, 1g yeast


Will check back once my transfers are done




I would drop your recipe even lower.

Try a few different recipes.

General Recipe is 10g/10/1g

I like to go: LME 7G/Agar 10G/1G Yeast

But if you are looking for structures and sectors, I would reduce even further




Thanks I'll give that a try. Baba was saying "1.5g-2g agar and 1%-2% Malt extract on 100ml water"

So maybe: LME 6G / Agar 6G / 1G yeast?



  Less yeast on a recipe like that.

Baba wouldn’t lead you astray.

:takingnotes:


--------------------

Meditation is passive learning. Tabernacling with the one infinite Creator is passive learning. It is the learning from listening to the voice one cannot hear which answers questions which one cannot ask. The process cannot be known and cannot come into consciousness in local sense, yet the learning is there. And the strength of spirit and will which comes from this learning is helpful.
.

:egyptian:www.templeofhermes.org:egyptian:


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