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Melgo
Semperviva Fanatic



Registered: 02/11/16
Posts: 698
Loc: Western Europe
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Re: Official Ps. Subtropical/Semperviva Thread [Re: offworld]
#28400498 - 07/19/23 09:13 AM (6 months, 26 days ago) |
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Quote:
offworld said: My first success with semps! I tried for over a year with a vendor syringe I could not clean up but received a print from a kind member here and very excited to see a flush.

Ive enjoyed seeing the timelines folks are providing so here are mine:
day 0: 1qt spawn to CV (bucket prep, slightly below FC) 1:2, placed in water tub day 7: cased with pasteurized cactus soil, just enough to cover surface day 15: pins present day 21: moved from water tub, dub-tubbed and placed on shelf - misted occasionally because I 2nd guessed myself moving them out of the water tub but space was getting tight there day 29 (today): spores present on earliest to pin - expect the other half to show spores in the next day or so
Thank you 0t0l for the spores and to everyone for the valuable info provided on this forum, special callout to Melgo and Moopers for their guides which helped this noob tremendously.

14 days from pins to spores! That's fast!!!
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MojaveMyc
Fractal Farmer



Registered: 05/08/21
Posts: 688
Loc: Colorado
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Melgo] 1
#28400572 - 07/19/23 10:34 AM (6 months, 26 days ago) |
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Congrats, offworld! Looking forward to the trip report
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offworld
Lurker


Registered: 08/07/21
Posts: 71
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Re: Official Ps. Subtropical/Semperviva Thread [Re: MojaveMyc] 1
#28400604 - 07/19/23 11:05 AM (6 months, 26 days ago) |
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Quote:
MojaveMyc said: Congrats, offworld! Looking forward to the trip report 
Cannot wait! So excited from all I've heard here.
-------------------- LAGM v. 2.023
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Adas
Lonely Dreamer



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Re: Official Ps. Subtropical/Semperviva Thread [Re: Melgo]
#28400688 - 07/19/23 12:13 PM (6 months, 25 days ago) |
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Quote:
Melgo said: Last night, I was thinking about one "quick" experiment, that we could pretty much all do:
- inoculate a jar of your grain of your choice with Semp mycelium (grain only, no straw, no coir, nothing but grain).
- Wait for it to fully colonize.
- Case it with pasteurized cactus soil or something close to it.
- Place in: Martha, JCM chamber, water tub, maybe SirPsycho domes, etc.
- Wait for pins to appear, write down how long it takes.
I have a bastard version of a JCM chamber atm. I'm trying to dial it in, so far not great because fruits aren't developing very well. But pinning is constant. And with Semp: again 9 days after casing I get pins. Doesn't matter the print so far.
Doing so, we get down to the "minimum" - I think.
In my old automated FC, I got amazing results such as this (old photos - wheat cased with pasteurized cactus soil):

It's fairly quick because your jar will be colonized in 20 days, cased and maybe 9 days later: pins - who knows? 
Is this to minimize as many variables as possible and see if fruiting times have anything to do with conditions?
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0t0lerance


Registered: 05/01/21
Posts: 962
Loc: The Netherlands
Last seen: 22 days, 3 hours
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Re: Official Ps. Subtropical/Semperviva Thread [Re: offworld] 2
#28400723 - 07/19/23 12:53 PM (6 months, 25 days ago) |
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Quote:
offworld said:
Quote:
MojaveMyc said: Congrats, offworld! Looking forward to the trip report 
Cannot wait! So excited from all I've heard here.
My pleasure to help you out man, hope you encounter the devine the stuff of love nice grow! Do a small dose first to check potency.. these can deceive..
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Melgo
Semperviva Fanatic



Registered: 02/11/16
Posts: 698
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Last seen: 2 hours, 27 minutes
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Adas]
#28400772 - 07/19/23 01:52 PM (6 months, 25 days ago) |
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Quote:
Adas said: Is this to minimize as many variables as possible and see if fruiting times have anything to do with conditions?
Yes indeed. Conditions and supposed (fast? slow?) lineage.
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Adas
Lonely Dreamer



Registered: 12/22/16
Posts: 5,278
Loc: Central EU
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Melgo] 2
#28400775 - 07/19/23 01:55 PM (6 months, 25 days ago) |
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Interesting proposal, although I seriously doubt it'll have any effect on the truly slow variety (Ambrose and similar) that take 4 months to just pin. I gave both pretty much the same conditions.
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,756
Loc: State of Confusion
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Melgo] 2
#28400901 - 07/19/23 03:52 PM (6 months, 25 days ago) |
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Quote:
Melgo said: Last night, I was thinking about one "quick" experiment, that we could pretty much all do:
- inoculate a jar of your grain of your choice with Semp mycelium (grain only, no straw, no coir, nothing but grain).
- Wait for it to fully colonize.
- Case it with pasteurized cactus soil or something close to it.
- Place in: Martha, JCM chamber, water tub, maybe SirPsycho domes, etc.
- Wait for pins to appear, write down how long it takes.
I have a bastard version of a JCM chamber atm. I'm trying to dial it in, so far not great because fruits aren't developing very well. But pinning is constant. And with Semp: again 9 days after casing I get pins. Doesn't matter the print so far.
Doing so, we get down to the "minimum" - I think.
In my old automated FC, I got amazing results such as this (old photos - wheat cased with pasteurized cactus soil):

It's fairly quick because your jar will be colonized in 20 days, cased and maybe 9 days later: pins - who knows? 
Well Iβm already playing around, and have some grain jars at the ready, and some semperviva LC. You talked me into it. Iβll do two, one in my JCM set up for pans, and one in with the bags in my tent for gourmets.
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Melgo
Semperviva Fanatic



Registered: 02/11/16
Posts: 698
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Last seen: 2 hours, 27 minutes
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Re: Official Ps. Subtropical/Semperviva Thread [Re: RoscoeReturns]
#28401252 - 07/19/23 11:12 PM (6 months, 25 days ago) |
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Quote:
Adas said: Interesting proposal, although I seriously doubt it'll have any effect on the truly slow variety (Ambrose and similar) that take 4 months to just pin. I gave both pretty much the same conditions.
Can you send me a print of the "truly slow variety (Ambrose and similar)" - please please please?? 
Quote:
RoscoeReturns said: Well Iβm already playing around, and have some grain jars at the ready, and some semperviva LC. You talked me into it. Iβll do two, one in my JCM set up for pans, and one in with the bags in my tent for gourmets.
Yeah man!
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0t0lerance


Registered: 05/01/21
Posts: 962
Loc: The Netherlands
Last seen: 22 days, 3 hours
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Melgo] 1
#28402419 - 07/21/23 03:01 AM (6 months, 24 days ago) |
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A man.. I lost the 120l tote to trichoderma... I don't understand.. 12 jars of really clean spawn (6 quart - 2 bricks coir).. everything was pasteurized properly with probes.. I've been really anal about keeping things clean.. clean the container.. lid.. heck even the liner cleaned with isopropyl alcohol why does it contaminate..really really frustrating..
Edited by 0t0lerance (07/21/23 06:12 AM)
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psilocybist
All is One



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Loc: Far
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Re: Official Ps. Subtropical/Semperviva Thread [Re: 0t0lerance] 1
#28402485 - 07/21/23 05:03 AM (6 months, 24 days ago) |
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That was an oversized bet, sorry it went that way.
lol I wish I'd seen trich by now in my buckets, so I could dump them with clear conscience. But now I just have to wait and feel less hope each week. It's been 75 days since spawned to bulk.
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chris77
Archaic Revivalist



Registered: 01/17/22
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Re: Official Ps. Subtropical/Semperviva Thread [Re: psilocybist]
#28402516 - 07/21/23 05:45 AM (6 months, 24 days ago) |
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Quote:
psilocybist said: That was an oversized bet, sorry it went that way.
lol I wish I'd seen trich by now in my buckets, so I could dump them with clear conscience. But now I just have to wait and feel less hope each week. It's been 75 days since spawned to bulk.
-------------------- the observer is the observed j. krishnamurti
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0t0lerance


Registered: 05/01/21
Posts: 962
Loc: The Netherlands
Last seen: 22 days, 3 hours
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Re: Official Ps. Subtropical/Semperviva Thread [Re: psilocybist]
#28402545 - 07/21/23 06:21 AM (6 months, 24 days ago) |
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Quote:
psilocybist said: That was an oversized bet, sorry it went that way.
lol I wish I'd seen trich by now in my buckets, so I could dump them with clear conscience. But now I just have to wait and feel less hope each week. It's been 75 days since spawned to bulk.
Well, I have 7 jars left.. which consolidated for quite some time.. so I'll give it 1 more try spawn that to 1 brick... but this time I will not add such a thick top layer.. (p7 tek) and go Melgo's route..
I'll go for a bucket tek instead of pasteurization in the presto.. maybe the outer layer of the coir got too hot and didn't got recolonized by bacteria.. as I didn't gave it a few days to ripen post pasteurization.
OR
The grain was contaminated.. but didn't show in the jar.. I have it 1 week to consolidate, maybe it did harbor something.. but I highly doubt it.. all 20 jars ended up clean.
But yeah, feel you there 75 days is a long time.. how about scraping the casing, dunk 4h and recase? I recall that used to be something advised in the exotics thread a year back or so.
Edited by 0t0lerance (07/21/23 06:21 AM)
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psilocybist
All is One



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Posts: 531
Loc: Far
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Re: Official Ps. Subtropical/Semperviva Thread [Re: 0t0lerance]
#28402618 - 07/21/23 07:54 AM (6 months, 24 days ago) |
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WellΨ I tried a few tricks, shaked a few buckets and without breaking the sub apart or make it crack, slammed a few on ground. Also, as someone suggested to someone else here, I let the surface of a few buckets to dry up a little, and made them extra wet, (1 or 2 millimeters of standing water). The water got absorbed in a day or two. These were done around 10 days ago, still no pin.
I just might, mix all the buckets together and lay them in a big garden pot, hoping for some miracle by nature conditions, but it's too hot and dry now, and if no pin appears by autumn, will carry them there.
edit: Forgot to mention, also as a desperate try and to experiment with pH shocks, to see if that could trigger pinning, I sparkled some diatomaceous earth on 3 trays (which were colonized and cased earlier, and casing was fully colonized as well, and showed no pins in months). The powder covered all the surface. Then I sprayed water on it. These trays also haven't pinned so far.
Edited by psilocybist (07/21/23 08:01 AM)
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ReverendMyc

Registered: 03/29/19
Posts: 1,580
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Re: Official Ps. Subtropical/Semperviva Thread [Re: 0t0lerance]
#28402765 - 07/21/23 10:51 AM (6 months, 24 days ago) |
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Well that sucks.
I really like running a bunch of smaller tubs because you can isolate and test more variables without the commitment of a single large tub. I have still had to toss all 6 tubs on a different failed project, which sucks. But at least, I know 6 things that didn't work instead of just one.
Sorry for you loss, but you will get it. It took me 9 tries and and over a year to get these buggers to grow. But now I have a nice reliable culture and process for these and am starting a few more from other sources to compare.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more"Psychedelics are powerful substances. Nothing that powerful is completely safe... and nothing completely safe is that powerful!" - Abigail Calder at ALPS 2023 Don't Panic   
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Konbri



Registered: 10/16/17
Posts: 141
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Re: Official Ps. Subtropical/Semperviva Thread [Re: psilocybist]
#28402802 - 07/21/23 11:46 AM (6 months, 23 days ago) |
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Quote:
psilocybist said: That was an oversized bet, sorry it went that way.
lol I wish I'd seen trich by now in my buckets, so I could dump them with clear conscience. But now I just have to wait and feel less hope each week. It's been 75 days since spawned to bulk.
You shouldn't loose hope, I'm telling you, they always pin sooner or later. First wave can be ugly though, with those pins in the corner. All my jars and boxes are fruiting now, some were three or for months till pins
-------------------- Radio Gnome Invisible
   
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Telomere
Unravelling


Registered: 12/26/22
Posts: 183
Last seen: 49 minutes, 57 seconds
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Konbri] 1
#28402963 - 07/21/23 02:30 PM (6 months, 23 days ago) |
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I set my shoebox into a mono tub with a little water in the bottom and a single fogger disk about a week ago. Been dialling it in. The newer fruits caps arenβt curling up and the older fruits arenβt curling up any more than they already were.
However - over the last 2 days some of the older fruits stipes are bluing. Is this a sign that they are ready to take? Or maybe they arenβt as happy as I hoped they were? I dunno.
My question is though, what are the signs to look out for that tell us the fruits are ready to take?


Some of these new clusters are growing waaay faster than previous growth. Itβs amazing actually how resilient these things are. This box has taken quite a beating really.
Edited by Telomere (07/21/23 02:32 PM)
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
Posts: 6,948
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Telomere] 1
#28402976 - 07/21/23 02:37 PM (6 months, 23 days ago) |
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I believe the blue is a sign of too much FAE(drying up). I noticed it around where the holes in my domes are for bottle grows
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Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
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    "Mist your balls and fan your asshole" - Pandaskis, 2023
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Telomere
Unravelling


Registered: 12/26/22
Posts: 183
Last seen: 49 minutes, 57 seconds
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Re: Official Ps. Subtropical/Semperviva Thread [Re: SirPsycho]
#28403004 - 07/21/23 03:10 PM (6 months, 23 days ago) |
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Quote:
SirPsycho said: I believe the blue is a sign of too much FAE(drying up). I noticed it around where the holes in my domes are for bottle grows
That was my first assumption and it makes perfect sense. I took the mp tape off the hole closest to them a few days ago as a bit of an experiment and put it back on a day later.
Sir Psycho, how do you gauge when to pick your fruits? Most of these blue guys are a little over 2 weeks since they popped up.
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SirPsycho
Purple Belt in Google-Fu



Registered: 01/01/20
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Re: Official Ps. Subtropical/Semperviva Thread [Re: Telomere] 1
#28403007 - 07/21/23 03:12 PM (6 months, 23 days ago) |
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Quote:
Telomere said:
Quote:
SirPsycho said: I believe the blue is a sign of too much FAE(drying up). I noticed it around where the holes in my domes are for bottle grows
That was my first assumption and it makes perfect sense. I took the mp tape off the hole closest to them a few days ago as a bit of an experiment and put it back on a day later.
Sir Psycho, how do you gauge when to pick your fruits? Most of these blue guys are a little over 2 weeks since they popped up.
I'm after prints so I let them go until the gills are straight up black
--------------------
Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints Balance in life is like running on ice.
π
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    "Mist your balls and fan your asshole" - Pandaskis, 2023
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